• 제목/요약/키워드: Nested Factor

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Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

  • Qiao, Tian-Min;Zhang, Jing;Li, Shu-Jiang;Han, Shan;Zhu, Tian-Hui
    • The Plant Pathology Journal
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    • 제32권5호
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    • pp.414-422
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    • 2016
  • Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

Bayesian Hypothesis Testing for Intraclass Correlation Coefficient

  • Lee, Seung-A;Kim, Dal-Ho
    • Communications for Statistical Applications and Methods
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    • 제13권3호
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    • pp.551-566
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    • 2006
  • In this paper, we consider a Bayesian model selection for the intraclass correlation coefficient in familiar data. In particular, we compare two nested models such as the independence and intraclass models using the reference prior. A criterion for testing is the Bayesian Reference Criterion by Bernardo (1999) and the Intrinsic Bayes Factor by Berger and Pericchi (1996). We provide numerical examples using simulation data sets for illustration.

Bayesian Test for the Intraclass Correlation Coefficient in the One-Way Random Effect Model

  • Kang, Sang-Gil;Lee, Hee-Choon
    • Journal of the Korean Data and Information Science Society
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    • 제15권3호
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    • pp.645-654
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    • 2004
  • In this paper, we develop the Bayesian test procedure for the intraclass correlation coefficient in the unbalanced one-way random effect model based on the reference priors. That is, the objective is to compare two nested model such as the independent and intraclass models using the factional Bayes factor. Thus the model comparison problem in this case amounts to testing the hypotheses $H_1:\rho=0$ versus $H_2:{\rho}{\neq}0$. Some real data examples are provided.

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On Flexible Bayesian Test Criteria for Nested Point Null Hypotheses of Multiple Regression Coefficients

  • Jae-Hyun Kim;Hea-Jung Kim
    • Communications for Statistical Applications and Methods
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    • 제3권3호
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    • pp.205-214
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    • 1996
  • As flexible Bayesian test criteria for nested point null hypotheses of multiple regression coefficients, partial and overall Bayes factors are introduced under a class of intuitively meaningful prior. The criteria lead to a simple method for considering different prior beliefs on the subspaces that constitute a partition of the coefficient parameter space. A couple of tests are suggested based on the criteria. It is shown that they enable us to obtain pairwise comparisons of hypotheses of the partitioned subspaces. Through a Monte Carlo simulation, performance of the tests based on the criteria are compared with the usual Bayesian test (based on Bayes factor)in terms of their respective powers.

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중년 남성에서 고정상혈압에 의한 고혈압발생 위험 규명을 위한 코호트내 환자-대조군 연구 (A Nested Case-Control Study on the High Normal Blood Pressure as a Risk Factor of Hypertension in Korean Middle-aged Men)

  • 안윤옥;배종면
    • Journal of Preventive Medicine and Public Health
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    • 제32권4호
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    • pp.513-525
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    • 1999
  • Objectives : High-normal blood pressure' is a factor influencing decision to initiate targeted intensive intervention strategy in westernized populations. JNC-VI offered the vigorous lifestyle modification for persons with 'high-normal blood pressure', who could be early detected. As a hypertension seems to be the result of multiple genetic factors operating in concert with associated environmental factors, it will be necessary to identify the high-normal blood pressure as a risk factor of hypertension for applying primary prevention strategy in Korean people. Methods : Although cohort study design might be adequate to recruit incidence cases, to keep time sequence of events, and to prevent information bias, nested case-control study was chosen for avoiding measurement errors because hypertension is a benign disease. Source population was the 'Seoul Cohort' participants and follow-up was done by using Korea Medical Insurance Corporation's database on the utilization of health services from 1 Jan93 to 30Jun97. Incidence cases were ascertained through the chart review, telephone contacts, and direct blood pressure measurements. Controls included the pairing of 4 individuals to each case on the basis of age. Results : As 75% of 247 incident cases had high-normal blood pressure, the crude odds ratio for hypertension was 2.04 (95% CI 1.47-2.83). Another statistically significant risk factors of hypertension were body mass index, dietary fiber, alcohol consumption, weekly activity and history of quitting smoking. The multivariate odds ratio of high-normal blood pressure adjusted for all risk factors was 1.84 (95% CI 1.31-2.56). Among high-normal blood pressure group, body mass index, weekly ethanol amounts, weekly physical activity, and dietary fiber except history of quitting smoking were still risk factors of hypertension. Conclusion : 'High-normal blood pressure' is a risk factor for hypertension in Korean middle-aged men, which represents that the vigorous lifestyle modification for persons with 'high-normal blood pressure' is need.

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A Marginal Probability Model for Repeated Polytomous Response Data

  • Choi, Jae-Sung
    • Journal of the Korean Data and Information Science Society
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    • 제19권2호
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    • pp.577-585
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    • 2008
  • This paper suggests a marginal probability model for analyzing repeated polytomous response data when some factors are nested in others in treatment structures on a larger experimental unit. As a repeated measures factor, time is considered on a smaller experimental unit. So, two different experiment sizes are considered. Each size of experimental unit has its own design structure and treatment structure, and the marginal probability model can be constructed from the structures for each size of experimental unit. Weighted least squares(WLS) methods are used for estimating fixed effects in the suggested model.

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Helicobacter pylori 감염와 위암과의 관련성에 대한 식이요인의 교호작용: 코호트내 환자-대조군 연구 (Effect modification of association between Helicobacter pylori infection and gastric cancer by dietary factor : Result from a nested case-control study within cohort)

  • 곽진;신애선;신해림;강대희;박수경;김청식;유근영
    • 대한예방의학회:학술대회논문집
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    • 대한예방의학회 2004년도 제56차 추계 학술대회 연제집
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    • pp.81.2-81.2
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    • 2004
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한국 웩슬러 유아지능검사 4판(K-WPPSI-IV)의 지능구조에 관한 연구 (A Study on the Structure of Intelligence Measured by the K-WPPSI-IV)

  • 이경옥;박혜원;이상희
    • 아동학회지
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    • 제37권6호
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    • pp.107-117
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    • 2016
  • Objective: This study examined the construct validity of K-WPPSI-IV. Factor structures of the structures of the K-WPPSI-IV full scale as well as primary index scales for two age ranges (2 years, 6 months to 3 years, 11 months; 4 years to 7 years, 7 months) were examined. Methods: Data were collected from 1,700 children aged 2 years, 6 months to 7 years, 7 months during the K-WPPSI-IV standardization. Confirmatory factor analyses were conducted using the K-WPPSI-IV subtest performances with maximum likelihood estimation using Amos 18. Results: First, the three-factor model (verbal comprehension, visual spatial, and working memory) fitted best for the younger age range. However, the five-factor model (verbal comprehension, visual spatial, fluid reasoning, working memory, and processing speed) fitted best for the older age range. Residuals suggest the presence of two nested subfactors within the verbal comprehension factor (broad/expressive and focused/simple). Second, the confirmatory factor analysis on primary index subtests identified factors that account for the intercorrelations among the reduced sets of primary index subtests. Conclusion: The findings showed that the theoretical structures of WPPSI-IV subtests were confirmed within K-WPPSI-IV.

랜덤화 순서와 기하학적 특성을 고려한 분산분석과 실험계획의 응용방안 (Application of ANOVA and DOE by Using Randomized Orders and Geometrical Properties)

  • 최성운
    • 대한안전경영과학회:학술대회논문집
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    • 대한안전경영과학회 2012년 춘계학술대회
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    • pp.277-292
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    • 2012
  • The research presents an application of Balanced ANOVA (BANOVA) by utilizing randomized orders for various Split-Plot Designs (SPDs) which include two cell designs, split-plot with one-way HTC (Hard to Control) factor, split-plot with two-way HTC factor, split-split-plot design and nested design. In addition, four MINITAB examples of 2-level split-plot designs based on the number of blocks and the type of whole-plots are presented for practitioners to obtain comprehensive understanding. Furthermore, the geometrical interrelated properties among three typical Designs of Experiments (DOE), such as Factorial Design (FD), Response Surface Design (RSD), and Mixture Design (MD) are discussed in this paper.

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