• Journal of fish pathology
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• v.14 no.2
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• pp.91-95
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• 2001

Mass mortalities occurred among red drum larvae, 20 to 30 days old, culturing at hatcheries on southern costal area. No specific external signs were observed except abnormal swimming and spinal deformity. It was, however, suspected as a viral etiology due to high mass mortalities so that histopathological and molecular biological study was performed to evaluate the agent. Both vacuoles and necrosis were observed on nerve cells of brain and eye by H-E staining, and viral particles were observed on electronmicroscopic examination. On the other hand, DNA fragment, approximately 426 bps, was amplified with RT-PCR. The above results were able to diagnosis the etiological agent of mass moralities in red drum larvae as VNN(viral nervous necrosis)virus.

• The Korea Journal of Herbology
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• v.20 no.2
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• pp.115-125
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• 2005

Objectives : Polygalae Radix (PR) from Polygalae tenuifolia (Polygalaceae) has been clinically used as a sedative, anti-inflammatory, and anti-bacterial agent. To extend pharmacological effects of PR in the central nervous system (CNS) on the basis of its CNS protective effect, the present study was conducted to identify the effect of PR, whether it shows the neuroprotective action against excitatory neurotoxicity. Methods : To identify the protective effect of PR to excitatory neuro-toxic agent, the present study was focused on the PR effect on cell death, that was caused by applying NMDA to nerve cell, elevation of $(Ca^{2+})_i$, releasement of glutamate, and ROS generation. Result : 1. PR methanol extract, at the concentration range of 0.05 to 5 g/ml, significantly inhibited NMDA (1 mM)-induced neuronal cell death as well as MK-801 (non competitive NMDA antagonist). 2. PR methanol extract $(0.5\;{\mu}g/ml)$ inhibited NMDA (1 mM)-induced elevation of cytosolic calcium concentration $[Ca^{2+}]_i$. NMDA application in the presence of MK-801 $(10\;{\mu}M)$ failed to produce the increase of $[Ca^{2+}]_i$ through all the measurement time. 3. PR methanol extract $(0.5\;{\mu}g/ml)$ inhibited the NMDA-induced elevation of glutamate release. Also, MK-801 showed similar protective effects. 4. PR methanol extract $(0.5\;{\mu}g/ml)$ inhibited the NMDA-induced elevation of ROS generation. Also, MK-801 showed similar protective effects. Conclusion : The present study provides the availability of PR to exert its protective effect on the neuronal cell death in various neurodegenerative pathophysiological conditions.

• Journal of the Korea Institute of Military Science and Technology
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• v.25 no.4
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• pp.434-442
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• 2022

Highly toxic chemical warfare agents(CWA) could be used in chemical warfare and terrorism. The protection of skin is crucial for civilians and soldiers, because the primary routes of exposure to CWA are inhalation and skin absorption. Thus, topical skin protectants(TSP) have been studied and developed in many countries to complement protective equipments. In this study, in-vitro test procedure was optimized and established using a flow-through diffusion cell system containing excised hairless mouse skin in an attempt to assess the effectiveness of various TSP formulations against nerve agent simulants. In addition, the test results on the formulations including the ingredients used in SERPACWA(Skin Exposure Reduction Paste Against Chemical Warfare Agent) and IB-1(TSP of Israel) were included, indicating that the formulations with perfluorinated compounds were more effective than the glycerin-based formulations.

• Korean Chemical Engineering Research
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• v.45 no.2
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• pp.172-177
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• 2007

The hydrolysis reaction of sarin(GB), one of the nerve agents was studied in aqueous sodium hydroxide(NaOH) solutions to find the experimental conditions which can convert GB into the less toxic compounds. 10 wt% of GB was added into the aqueous NaOH(2.05 eq) in a small-scale jacket-attached reactor connected to a circulator. The reaction rate constants were measured at three temperatures(50, 70 and $90^{\circ}C$) and the reaction times required to degrade the material to > 99% were calculated at different temperatures. In this study, 10 wt% of GB was degraded to 99.99% in 1.2hr at $90^{\circ}C$ by the aqueous NaOH solution. The major hydrolysate of GB was isopropyl methylphosphonate.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.13 no.2
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• pp.164-172
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• 2002

The role of botulinum toxin as a therapeutic agent is expanding rapidly in otolaryngology. Botulinum toxin is a protease that blocks the release of acetylcholine from nerve terminals. Its effects are transient and nondestructive, and largely limited to the area in which it is administered These effects are also graded according to the dose, allowing for individualized treatment of patients and disorders. Botulinum toxin has been used primarily to treat disorders of excessive or inappropriate muscle contraction. In the field of otolaryngology, these include spasmodic dysphonia, oromandibular dystonia, and blepharospasm, vocal tics and stuttering, cricopharyngeal achalasia, various tremors and tics, hemifacial spasm, temporomandibular joint disorders and a number of cosmetic applications. Botulinum toxin treatment has recently begun to show some benefit in the control of pain from migraine and tension headache. It may also prove useful in the control of autonomic dysfunction, as in Frey syndrome, sialorrhea, and rhinorrhea. In over 20 yews of use in humans, botulinum toxin has accumulated a considerable safety record, and in many cases represents relief for thousands of patients unaided by other therapy.

• Archives of Plastic Surgery
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• v.33 no.3
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• pp.388-391
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• 2006

Hemangioma is one of the most common congenital tumors in the region of the face and neck. Although histologically benign, these facial masses are clinically malignant for their deforming and inexorable growth, especially in so-called 'cavernous hemangioma'. Carvenous hemangioma is the most common primary tumor occurring in the adult orbit. This tumor has symptoms that characteristically develop over several years with slowly progressive proptosis, eyeball deviation, hyperopia, diplopia and optic nerve compression. Today, hemangiomas are being treated by various methods; steroids, electrocoagulation, injection of sclerosing agent, cryotherapy, radiation therapy, laser therapy, and surgical treatment, etc. In principle, surgical approaches to the orbit must provide maximum safety and optimal visualization. We have experienced a case of large cavernous hemangioma in the orbit inferolaterally. The surgical treatment of tumor was achieved by the bicoronal approach combined with inferomedial and inferolateral orbitotomy. This surgical approach allows better visualization of the tumor and greater protection of essential anatomic structures. We obtained satisfactory results in terms of aesthetic and functional consideration. We present our case with a brief review of the literature related to orbital cavernous hemangioma.

• BMB Reports
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• v.49 no.5
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• pp.288-292
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• 2016

Cisplatin is a platinum-based chemotherapeutic drug for treating various types of cancers. However, the use of cisplatin is limited by its negative effect on normal tissues, particularly nephrotoxicity. Various mechanisms such as DNA adduct formation, mitochondrial dysfunction, oxidative stress, and apoptosis are involved in the adverse effect induced by cisplatin treatment. Several studies have suggested that neuropeptide Y (NPY) is involved in neuroprotection as well as restoration of bone marrow dysfunction from chemotherapy induced nerve injury. However, the role of NPY in chemotherapy-induced nephrotoxicity has not been studied. Here, we show that NPY rescues renal dysfunction by reducing the expression of pro-apoptotic proteins in cisplatin induced nephrotoxicity through Y1 receptor, suggesting that NPY can protect kidney against cisplatin nephrotoxicity as a possible useful agent to prevent and treat cisplatin-induced nephrotoxicity.

• Proceedings of the KAIS Fall Conference
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• 2009.05a
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• pp.13-17
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• 2009

In this thesis, we made a sheet to improve the concentration of study. To demonstrate the improvement in the concentration of study, we obtained functional magnetic resonance imaging (fMRI), which has superior time resolution and measures brain noninvasively by using intrinsic contrast agent. As a result of Brainwave measurement, we could verify the blood flow's activate in the nearby frontal lobe related to memory process and noticeable ratio change in absolute alpha wave and beta wave after the analysis of Brainwave measurement. fMRI ascertains the physiological function of the brain and is being used to prevent the trouble medically that can be caused before and after the operation. For the visibility of cranial nerve network, many researches will be carried out to develope the product which is related to brain like concentration of study.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.191-191
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• 2013

금속 산화물 촉매 입자는 특정한 파장에 의해서 활성화되면서 전자-정공 쌍을 생성한다. 광촉매원리를 이용하면 전자 정공 제공을 통해 기존의 물질 주위에 활성 라디칼을 생성하고 물질의 특성을 변화시킬 수 있다. 이런 독특한 특성을 이용한 금속산화물의 다양한 연구가 물리, 화학, 재료, 생명 분야에서 이루어지고 있다. 본 연구에서는 광촉매 입자와 대기압 플라즈마와의 특성을 활용하여 발생되는 물리적 특성과 재료적인 특성을 이용한 응용 연구에 대한 내용을 다루고 있다. 특히 광촉매로 가장 많이 사용되는 $TiO_2$가 $200^{\circ}C$ 이하 저온 플라즈마 방전가스에 의해 상변화되는 현상을 다루고 이에 대한 구체적인 재료 분석을 실시 하였다. 즉, 저온의 알곤과 알곤/산소 대기압 플라즈마에 의해 처리된 $TiO_2$의 결정성 변화에 대해서 조사하였고 이를 이용하여 유사 작용제의 분해에 대한 연구를 하였다. 신경작용제(VX: nerve agent)의 유사작용제인 말라치온(Malathion)뿐만 아니라 셀룰로우즈(cellulose) 계의 복잡한 구조의 화학유기물 등을 대기압 플라즈마를 이용해 분해시킬 수 있음을 알 수 있었다. 본 연구에서는 대기압 플라즈마와 금속산화물의 결정성 변화에 대한 분석을 통해 기능성화된 촉매입자를 이용한 효과적인 화학물질의 분해를 소개하고, 대기압 플라즈마의 나노 소재기술로의 높은 응용가능성도 함께 살펴보았다.

• Biomedical Science Letters
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• v.26 no.2
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• pp.57-65
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• 2020

Excessive drinking of alcohol is known to be one of the main causes of various neurological diseases, such as Alzheimer's disease. Scopoletin is known to have anti-inflammatory and antioxidative properties, and to protect nerve cells. This study examined whether scopoletin inhibits the alcohol-induced apoptosis of primary hippocampal neurons, and how scopoletin regulates several factors associated with the caspase-mediated pathway. To achieve this, the cell viability and apoptosis rate of primary hippocampal neurons were measured by Cell Counting Kit-8 and flow cytometry, respectively. Apoptosis-related protein expressions (Bax, Bid, caspase-3, caspase-9, and Poly (ADP-ribose) polymerase (PARP)) were analyzed by Western blotting, and the ANOVA method was used to confirm the significance of the measured results. As a result, scopoletin inhibited the expressions of alcohol-induced apoptosis and apoptosis-related proteins in primary hippocampal neurons. These results suggest that down-regulation of Bid, Bax, and cleaved caspase-9 expression induced by scopoletin down-regulates the expression of cleaved caspase-3, inhibits the expression of cleaved PARP, and finally, inhibits mitochondrial apoptotic pathways. The study suggests that scopoletin is worth developing as a candidate for neuroprotective agent.