• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.392-395
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• 2005

A three-year old intact female German shepherd dog weighing 32kg was presented with the history of regurgitation and vomiting. Physical examination revealed a thin body condition, hoarse voice, hypersalivation, and pelvic limb weakness. In radiograph, meagaesophagus was identified. The neostigmine challenge test was positive. The titer of acetylcholine receptor (AChR) antibody was 1.58 nmol/L (reference range, <0.6nmol). Therefore, the dog was diagnosed to be suffering with chronic generalized form of acquired myasthenia gravis and followed by treatment with pyridostigmine bromide, 1mg/kg, q12 hrs. Treatment improved the pelvic limb weakness, however intermittent regurgitation and vomiting persisted. This resulted in aspiration pneumonia f3r which antibiotics were administered However, the dog was euthanized due to poor management and owner's request.

• Integrative Medicine Research
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• v.6 no.2
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• pp.149-155
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• 2017

Background: Naringenin (NRG) is a common dietary polyphenolic constituent of fruits. NRG has diverse pharmacological activities, and is used in traditional medicine to treat various diseases including gastrointestinal (GI) disorders. Interstitial cells of Cajal (ICCs) are pacemaker cells of the GI tract. In this study, the authors investigated the effects of NRG on ICCs and on GI motility in vitro and in vivo. Methods: ICCs were dissociated from mouse small intestines by enzymatic digestion. The whole-cell patch clamp configuration was used to record pacemaker potentials in cultured ICC clusters. The effects of NRG on GI motility were investigated by calculating percent intestinal transit rates (ITR) using Evans blue in normal mice. Results: NRG inhibited ICC pacemaker potentials in a dose-dependent manner. In the presence of tetraethylammonium chloride or iberiotoxin, NRG had no effect on pacemaker potentials, but it continued to block pacemaker potentials in the presence of glibenclamide. Preincubation with SQ-22536 had no effect on pacemaker potentials or on their inhibition by NRG. However, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one blocked pacemaker potential inhibition by NRG. In addition, L-NG-nitroarginine methyl ester blocked pacemaker potential inhibition by NRG. Furthermore, NRG significantly suppressed murine ITR enhancement by neostigmine in vivo. Conclusion: This study shows NRG dose-dependently inhibits ICC pacemaker potentials via a cyclic guanosine monophosphate/nitric oxide-dependent pathway and $Ca^{2+}$-activated $K^+$ channels in vitro. In addition, NRG suppressed neostigmine enhancement of ITR in vivo.

• Journal of Chest Surgery
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• v.13 no.3
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• pp.301-305
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• 1980

Myasthenia gravis is a disorder that affects neuromuscular transmission in a way that is still poorly understood. some think that myasthenia gravis results from a reduction of available acetylcholine receptors in neuromuscular junctions, consequent to some form of autoimmune injury. Surgical interest in this disease was first aroused in 1939 when Blalock observed that some patients with thymic tumors and myasthenia gravis improved following thymectomy. This report represents two cases of myasthenia gravis. The 14-year-old girl was admitted to Korea Universtiy Hospital with chief complaintment of bilateral ptosis, diplopia, swallowing difficulty, and mastication difficulty, which were relieved by administration of edrophonium (Tensilon) chloride, given intravenously. Myasthenica gravis was confirmed and thymectomy was given. After thymectomy, symptoms were relieved but the administration of neostigmine was contijued to be needed till following 3 months. After that period, she was free from this symptoms without anticholinesterase drugs. Second case is 57 year old male who has the symptoms of diplopia, bilatreal ptosis, walking disturbance, and speech difficulty. He had thymectomy too but in thymic tissue, malignant thymoma was included. He has subjective improvement only, with no major reduction of medication requirements after thymectomy.

• The Journal of Korean Medicine
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• v.32 no.6
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• pp.54-66
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• 2011

Objectives: The purpose of the present study was to investigate the antidiarrheal effects of Anjang-san in mice and rats. Methods: We measured the content of condensed tannin in Anjang-san extract, and observed the effects of Anjang-san on the small intestinal and colonic transport of mice, as well as on mice models of diarrhea induced by castor oil and $MgSO_4$, and on rat models of castor oil-induced enteropooling. Results: Anjang-san showed significant inhibitory effects on abnormally increased small intestinal transit induced by pyridostigmine and neostigmine, and inhibitory effects on large intestinal transit. Anjang-san also exhibited antidiarrheal effects on diarrhea induced by $MgSO_4$, and inhibitory effects on castor oil-induced enteropooling. Anjang-san also improved castor oil-induced diarrhea based on simple numbers without statistical significance. Conclusion: These results demonstrate that Anjang-san has significant antidiarrheal properties and attests to its possible utility in functional diarrheas, irritable bowel syndrome and other gastrointestinal disorders based upon further studies.

• Korean Journal of Veterinary Research
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• v.54 no.1
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• pp.59-62
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• 2014

A 12-year-old, castrated male Yorkshire terrier dog presented with frequent regurgitations that had begun 45 days earlier and become more progressive. Radiographs revealed an air-trap region behind the cranial esophageal sphincter muscle in the esophagus and esophagographies with barium contrast showed mild esophageal dilation with decreased motility. Esophageal motility increased within 5 min of neostigmine methylsulfate administration and acetylcholine receptor antibodies titer increased to beyond the normal range. Based on these findings, acquired myasthenia gravis with focal form was diagnosed, making this the first such case diagnosed by an acetylcholine receptor antibody test in Korea.

• Journal of Dental Anesthesia and Pain Medicine
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• v.23 no.5
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• pp.287-291
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• 2023

Sugammadex has shown faster reversal of steroidal neuromuscular blockade (NMB) than neostigmine, a traditional reversal agent for NMB, even in the intense block phase. This efficiency is possible because of the unique mechanism of action by encapsulating the NMB molecules. Therefore, with the use of sugammadex, we can also expect to avoid direct interactions with the cholinergic system and its subsequent side effects, which are disadvantages of traditional drugs. However, despite these benefits and US Food and Drug Administration (FDA) approval in 2015, rare adverse events associated with sugammadex have been reported. Herein, we report a case of bronchospasm that developed immediately after sugammadex administration.

• The Journal of Internal Korean Medicine
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• v.27 no.2
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• pp.356-370
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• 2006

Objectives: The aim of this experimental study is to investigate effects of Dolichoris Semen, Terminaliae Fructus and Bojanggunbi-tangon the anti-cathartic action of mice and guinea pigs to develop effective herbal medicine and prescription to cure diarrhea early and to prevent side effect of chronic diarrhea. Methods: To compare effects of Dolichoris Semen, Terminaliae Fructus and Bojanggunbi-tangon the anti-cathartic action of mice and guinea pigs, we evaluated the actions on isolated gastrointestinal smooth muscles. on the transportability of small and large intestines, onthe neostigmine-induced transportability of small intestine and on diarrhea induced by castor oil, pilocarpine, barium chloride. Results: (1) Dolichoris Semen, Terminaliae Fructus and Bojanggunbi-tang showed the strong alleviation on the contraction of mice's isolated gastrointestinal smooth muscles induced by acetylcholine chloride and barium chloride. while Terminaliae Fructus, strong, Bojanggunbi-tang, intermediate and Dolichoris Semenshowed the minor alleviation on the contraction of guinea pigs' isolated gastrointestinal smooth muscles induced by acetylcholine chloride and histamine. All effects depended on the density in each medicine. (2) The tranbportability of small intestine was contrained in the groups controlled by 600 mg/kg of Dolichoris Semen, Terminaliae Fructus and Bojanggunbi-tang, and 200 mg/kg of D olichoris Semen and Terminaliae Fructus. However, the enhancement of neostigmine-induced transportability of small intestine was constrained only in the groups controlled by 600 mg/kg of Dolichoris Semen, Terminaliae Fructus and Boianggunbi-tang. (3) The transportability of large intestine was constrained by Dolichoris Semen and Bojanggunbi-tang. They showed substantial results. which depended on the amount of each medicine. (4) Terminaliae Fructus showed substantial anti-cathartic effects on diarrhea induced by caster oil in both groups controlled by 200 and 600 mg/kg of it. However, Bojanggunbi-tangshowed the effects only in the group controlled by 600 mg/kg. in diarrhea induced by pilocarpine, the groups controlled by 600 mg/kg or Terminaliae Fructus and 200 and 600 mg/kg of Boianggnbi-tangshowed substantial results. In diarrhea induced by barium chloride. the groups controlled by 200 and 600 mg/kg of Terminaliae Fructus and 600 mg/kg of Boianggunbi-tangshowed substantial results in anti-cathartic effects. Conclusions: These results show that Terminaliae Fructus is more effective on the contraction of mice and guinea pigs' isolated gastrointestinal smooth muscles, Dolichoris Semen is more effective on the transportability of small intestine, Dolichoris Semen and Bojanggunbi-tang are more effective on the transportability of large intestine. Terminaliae Fructus and Bojanggunbi-tang show substantial results in anti-cathartic actions.

• Journal of Veterinary Clinics
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• v.29 no.1
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• pp.107-111
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• 2012

Three dogs (An 8 years-old intact female Poodle, a 7 years-old intact male Schunauzer, and an 8 yearsold Golden Retriever) were presented due to acute vomiting, dyspnea, and generalized weakness. Megaesophagus was confirmed through radiographic examination in all 3 dogs. Relative oesophageal diameter (ROD) was measured and results of ROD measurements showed the possibility of megaesophagus secondary to myasthenia gravis in three dogs. Thus we performed anticholinesterase test as screening test for myasthenia gravis. In all three dogs, esophageal diameter was reduced after neostigmine methylsulfate administration. For definite diagnosis of acquired myasthenia gravis, serum acetylcholine receptor antibody titer was measured, but definite diagnosis was confirmed only in one case. However, based on history, radiographic findings, anticholinesterase test, ROD measurement, other two cases were still suspected as megaesophagus secondary to myasthenia gravis. Treatment with pyridostigmine bromide was initiated in all dogs, and improvement of esophageal diameter was shown in all dogs. One dog was successfully managed for 15 months after initial treatment and, is still alive, but other two dogs were died shortly after initial treatment, because of severe aspiration pneumonia.

• The Journal of Internal Korean Medicine
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• v.27 no.2
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• pp.459-470
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• 2006

Objectives: The aim of this experimental study is to investigate the effects of MYRISTICAE SEMEN, GRANATI PERICARPIUM and ALPINIAE KATSUMADAI SEMEN on the anticatharsis by using mice and Guinea pigs. Methods : We evaluated the effect of herbs on the extracted organs contraction, the barium sulfate transport, and the anticathartic action induced by acetylcholine chloride, barium chloride, histamine chloride, neostigmine, castor oil, pilocarpine in the ileums, large intestines, small intestines of animals. Results : In the extracted organs, all herbs showed concentration-dependent inhibitory effect on the constriction of extracted ileum. The transport of barium sulfate in the large and small intestine was increased in comparing with the control group. And every three herbs showed the anticathartic action. Conclusions : These results demonstrate that MYRISTICAE SEMEN, GRANATI PERICARPIUM and ALPINIAE KATSUMADAI SEMEN extracts have an advantageous effects on diarrhea induced by drugs and will contribute to the development of diarrhea treatment through further related studies.

• Korean Journal of Veterinary Research
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• v.46 no.1
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• pp.71-73
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• 2006

A 7-month-old female Shih-tzu dog was presented with intermittent trembling, dyspnea, generalized muscle weakness, and unconsciousness after exercise. No remarkable findings were shown in the complete blood counts and the radiographic examination. On serum biochemical profiles, alkaline phosphatase and creatine phosphokinase were mildly elevated. Based on history takings, physical examination, and neurological findings, presumptive diagnosis was made as a myasthenia gravis (MG). Clinical signs of this patient were dramatically improved after administration of neostigmine. The result of acetylcholine receptor antibody test in serum was 0.89 nmol/L and the histopathology of muscle were normal. Clinical sign of the patient evaluated in this study is stabilized with long-term administration of pyridostigmine at this time. This case report here describes clinical and clinicopathological findings of a juvenile onset acquired MG in a Shih-tzu dog.