• Journal of Digital Convergence
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• v.16 no.3
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• pp.395-405
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• 2018

The purpose of this study is to investigate the effects of parental rearing attitudes on smartphone addiction and mediation effect of children's aggression and impulse between them. The randomly chosen 390 subjects of the 4th and 5th elementary school students (boy: 199, girl: 191) of J City participated in this research. The main scales were EMBU-Shot, Smartphone Addiction Scale and NEO-PAS. Spss 22.0 was used for its statistical analysis, that is correlation analysis and regression analysis. The main results are as follows. First, parental rearing attitude, smartphone addiction, and aggression and impulse were statistically significantly correlated. Second, it is testified that rejective parental rearing attitudes statistically significantly increasingly influenced smartphone addiction with partial mediation of aggression and impulse. Also overprotective parental rearing attitudes statistically significantly increasingly influenced smartphone addiction with full mediation of them. It suggested the importance of intervention for aggression and impulse of the children with smartphone addiction as well as parental rearing attitude changes in intervening and counseling them. This study tried to supply evidence-based and basic data for the developing preventive education and counseling programs for them. The limit and future tasks were discussed.

• The Journal of Korean Society of Virology
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• v.29 no.1
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• pp.33-38
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• 1999

Retroviral vector provide a highly efficient method for gene transfer into eukaryotic cells. This vector system can be divided into two components; the retroviral vector itself and the retroviral packaging cell line. The key improvement in the design of these two components are, focused on two aspects; the reduction of helper virus production and high titer-virus. We used PA317 for retrovirus packaging cell line, for its high producibility of viral titer. To test the ability of the vectors to generate high titer-virus, we have chosen four different retroviral vectors; LN, LNSX, LNCX and LXSN. To test easily the viral titer, we have made recombinant construction with CD4 and CD8, checked their viral titer and stained their surface expression. LXSN which contain SV40 early promoter in front of neo gene showed best results in viral transient transfection assay, dot blot assay and surface expression. In addition, recombinant containing CD8 generally showed much higher viral titration and surface expression than CD4.

• International Journal of Advanced Culture Technology
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• v.11 no.4
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• pp.385-392
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• 2023

The smart tourism service provides tourists with personal travel planner services and context-awareness-based tour guide services. In this paper, we propose the personal travel planner service that creates my travel itinerary using the smart tourism app and the travel planner system. The smart tourism app provides recommended travel products and POI tourist information used to create my travel itinerary. The smart tourism app also provides the smart tourism chatbot service that allows users to select POI tourist information easily and conveniently. The travel planner system consists of the smart tourism information system and the smart tourism chatbot system. The smart tourism information system provides users with travel planner services, recommended travel products, and POI tourism information through the smart tourism app. The smart tourism chatbot system consists of named entity recognition (NER), dialogue state tracking (DST), and Neo4J servers, and provides chatbot services as a smart tourism app. Users can create their own travel itinerary, modify the travel itinerary while traveling, and then register it as a recommended travel product to users, including acquaintances.

• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.347-351
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• 2002

Transgenes in HSY-TK gene driven by the lck promoter was tested for the expression in immune cells (Jurkat cells) to apply xenotransplantation of human cells into transgenic animals for the potential use of the proliferation or differentiation of human stem cells in the large animal such as an pig. Also, lck-CFP gene was used for transfection experiment into Jurkat cell to confirm the proper regulation of lck promoter for transgene expression in the T cells. Transfection of lck-GFP gene into Jurkat ceils induced CFP expression in transfected cells. The expression of Ick-TK and Ick-CFP genes was confirmed by RT-PCR using RNAs extracted from Jurkat cells, When Jurkat cells transfected with TK and CFP genes were selected against G418 or gancyclovir treatments, Jurkat cells transfected with TK gene were not proliferated in G4i8 and gancyclovir medium while intact cells or cells transfected with CFP gene could grow in gancyclovir medium. However, Jurkat cells transfected with TK or GFP gene were proliferated in G418 medium probably due to Neo$^{r}$ gene in the vector. Gancyclovir treatment destroyed Jurkat cells expressing TK gene indicating that T-cells expressing TK gene can be selectively eliminated by TK gene expression driven by lck promoter.

• International Journal of Internet, Broadcasting and Communication
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• v.14 no.2
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• pp.55-62
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• 2022

A smart tourism chatbot is needed as a user interface to efficiently provide smart tourism services such as recommended travel products, tourist information, my travel itinerary, and tour guide service to tourists. We have been developed a smart tourism app and a smart tourism information system that provide smart tourism services to tourists. We also developed a smart tourism chatbot service consisting of khaiii morpheme analyzer, rule-based intention classification, and tourism information knowledge base using Neo4j graph database. In this paper, we develop the Korean and English smart tourism Name Entity (NE) datasets required for the development of the NER model using the pre-trained language models (PLMs) for the smart tourism chatbot system. We create the tourism information NER datasets by collecting source data through smart tourism app, visitJeju web of Jeju Tourism Organization (JTO), and web search, and preprocessing it using Korean and English tourism information Name Entity dictionaries. We perform training on the KoBERT-CRF NER model using the developed Korean and English tourism information NER datasets. The weight-averaged precision, recall, and f1 scores are 0.94, 0.92 and 0.94 on Korean and English tourism information NER datasets.

• Korean Journal of Animal Reproduction
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• v.27 no.1
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• pp.25-34
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• 2003

Human thrombopoietin (hTPO) is a cytokine that plays a central role in megakaryopoiesis. To direct hTPO expression in the mammary gland, an expression vector was constructed by combining the promoter of bovine beta-casein gene, cDNA of hTPO and neomycin resistance gene (pBT-L neo). Fibroblast cells derived from cow's ear skin tissue were transfected with the expression vector (pBT-L neo) using Lipofectamine. Transfected cells resistant to G418 trea？nt were cultured to form the colonies for more than 2 weeks. The transformed colonies identified by PCR were further expanded prior to nuclear transfer. Reconstructed oocytes with transformed cells were electrofused, activated using calcium ionophore and 6-DMAP, and cultured in vitro for 7 days. Of 35 cell colonies analyzed by PCR, 29 colonies (82.9%) were positive for the hTPO gene. Cleavage and developmental rates to the blastocyst stage of reconstructed embryos with the transformed cells were 65.1% and 23.8%, respectively Of 29 blastocysts that developed from reconstructed embryos with the transformed cells, 27 embryos (93.1%) were transgenic. These results indicate that transgenic bovine embryos can be efficiently produced by somatic cell nuclear transfer using transformed cells.

• Biomedical Science Letters
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• v.16 no.4
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• pp.365-376
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• 2010

The purpose of this study was to clarify the effect of light emitting diode (LED) irradiation on healing of impaired wound and alteration of mast cells in experimental diabetic rats. Twenty-four male Sprague-Dawley rats were divided into four groups: excision (Ex), excision-LED irradiation (Ex-LED), diabetes + excision (DM) and diabetes + excision + LED irradiation (DM-LED). Diabetes was induced in rats by streptozotocin (STZ) injection (70 mg/kg, single dose) and 6 mm punch excision wounds were created on the back after shaving hair. The LED-irradiated rats were treated to a daily dose of $5\;J/cm^2$ LED (630 nm) light for 11 days after surgery, and were killed at day 1, 3, 7 and 11. The lesion and adjacent skin tissues were excised, fixed with 10% buffered formalin and embedded with paraffin. For evaluation of wound healing, hematoxylin-eosin (HE) and Masson trichrome staining were performed. Mast cells (MCs) were stained with toluidine blue (pH 0.5) and quantified using a computerized image analysis system. The proliferation activity of keratinocyte in skin tissues was analyzed on sections immunostained with proliferative cell nuclear antigen (PCNA). The results showed that wound healing rate, collagen density and neo-epidermis length, number of PCNA-positive cells, fibroblasts and mast cells were significantly higher in the LED-irradiated rats than in the DM and Ex rats throughout the periods of experiment. Exceptionally, the number of MCs was significantly lower at day 11 compared with day 7 after surgery in the all groups. These findings suggest that the LED irradiation may promote the tissue repair process by accelerating keratinocyte and fibroblast proliferation and collagen production in normal rats as well as in diabetic rats, and MCs may play an important role at an early stage of skin wound healing in normal and diabetic rats.

• Proceedings of the Korea Information Processing Society Conference
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• 2018.10a
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• pp.984-987
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• 2018

In an Internet of Thing (IoT) environment, entities with different attributes and capacities are going to be connected in a highly connected fashion. Specifically, not only the mechanical and electronic devices but also other entities such as people, locations and applications are connected to each other. Understanding and managing these connections play an important role for businesses, which identify opportunities for new IoT services. Traditional approach for storing and querying IoT data is used of a relational database management system (RDMS) such as MySQL or MSSQL. However, using RDMS is not flexible and sufficient for handling heterogeneous IoT data because these data have deeply complex relationships which require nested queries and complex joins on multiple tables. In this paper, we propose a graph model for constructing a graph database of heterogeneous IoT data. Graph databases are purposely-built to store highly connected data with nodes representing entities and edges representing the relationships between these entities. Our model fuses social graph, spatial graph, and things graph, and incorporates the relationships among them. We then present a case study which applies our model for representing data from a Smart Campus using Neo4J platform. Through the results of querying to answer real questions in Smart Campus management, we show the viability of our model.

• Knowledge Management Research
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• v.25 no.2
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• pp.1-18
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• 2024

With the increasing demand for and utilization of large language models (LLMs), the risk of user sensitive information being inputted and leaked during the use of LLMs also escalates. Typically recognized as a tool for mitigating the hallucination issues of LLMs, knowledge graphs, constructed independently from LLMs, can store and manage sensitive user information separately, thereby minimizing the potential for data breaches. This study, therefore, presents a knowledge graph-based chatbot that transforms user-inputted natural language questions into queries appropriate for the knowledge graph using LLMs, subsequently executing these queries and extracting the results. Furthermore, to evaluate the functional validity of the developed knowledge graph-based chatbot, performance tests are conducted to assess the comprehension and adaptability to existing knowledge graphs, the capability to create new entity classes, and the accessibility of LLMs to the knowledge graph content.

• Korean Journal of Digital Imaging in Medicine
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• v.11 no.2
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• pp.115-119
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• 2009

I think this will be valuable reference for assuring consistency and homogeneity of clarity and managing dental radiation equipment by experimentation of dental radiation equipment permanent which based on KS C IEC 61223-3-4 standard and KS C IEC 61223-2-7. Put a dental radiation generator and experiment equipment as source and film(sensor) length within 30 em, place the step-wedge above the film(sensor). Tie up tube voltage 60 kVp, tube current 7 mA and then get an each image through CCD sensor and film by changing the exposure time as 0.12sec, 0.25sec, 0.4sec. Repeat the test 5times as a same method. Measure the concentration of each stage of film image, which gained by experiment, using photometer. And the image that gained by CCD sensor, analyze the pixel value's change by using image J, which is analyzing image program provided by NIH(National Institutes of Health). In case of film, while 0.12sec and 0.25sec show regular rising pattern of density gap as exposure time's increase, 0.4sec shows low rather than 0.12sec and 0.25sec. In case of CCD sensor density test, the result shows opposite pattern of film. This makes me think that pixels of CCD's sensor can have 0~255 value but it becomes saturation if the value is over 255. The way that getting clear reception during decreasing human's exposed radiation is one of maintaining an equipment as a best condition. So we should keeping a dental radiation equipment's condition steadily through cyclic permanent test after factor examination. Even digital equipment doesn't maintain a permanent, it can maintain a clarity by post processing of image so that hard to set it as standard of permanent test. Therefore it would be more increase the accuracy that compare a film as standard image. Thus I consider it will be an important measurement to care for dental radiation equipment and warrant homogeneity, consistency of dental image's clarity through comparing pattern which is the result from factor test against cyclic permanent test.