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Evaluation of Enzyme-linked Immunosorbent Assay in Serological Diagnosis of Human Neurocysticercosis using paired Samples of Serum and Cerebrospinal Fluid (면역효소측정법을 이용한 뇌낭미충증의 혈청학적 진단의 평가)

  • 조승열;김석일
    • Parasites, Hosts and Diseases
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    • v.24 no.1
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    • pp.25-41
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    • 1986
  • The applicability of micro-ELISA was evaluated in human neuro-cysticercosis using paired samples of serum and CSF. A total of 355 cases who were mostly neurologic patients was subjected. Cystic fluid of C. cellulosae was used as antigen in protein concentration of $2.5{\;}{\mu}g/ml$. Serum was diluted to 1 : 100 and CSF was undiluted in the assay for the specific IgG antibody level. The differential criterion of the positive reaction was the abs. of o. 18 in both samples. The results were summarized as follows: 1. The overall sensitivity of the micro-ELISA in 71 confirmed neurocysticercosis was 90.1% ; the sensitivity by serum was 77.5% and that by CSF was 83.1%. CSF was a more sensitive and valuable material. Most of the false negative cases of neuro-cysticercosis showed far lower level of abs. rather than marginal. 2. The overall specificity of the micro-ELISA in 52 confirmed other neurologic diseases was 88.5%; the specificities by serum and by CSF were 94.2% respectively. Cases of other neurologic diseases did not show false positive reactions in both samples. 3. When serum was assayed, taeniasis(2/18), sparganosis(2/20), paragonimiasis(1/56), clonorchiasis(1/15) and fascioliasis(1/1) cases showed cross reactions. When CSF was assayed, 2 ot 10 neuro-sparganosis showed cross reactions while none of 9 neuro-paragonimiasis showed it. Out of 71 confirmed neuro-cysticercosis cases, 6 and 11 showed cross reactions by serum and CSF to crude extract antigen of sparganum; but no case did show it to crude extract antigen of Paragonimus westermani. 4. Ventricular CSF showed low or negative levels of IgG antibody than lumbar CSF unless the lesion was at the lateral ventricle itself. 5. Out of 4 racemose cysticercosis cases, 3 showed positive reaction in serum while all of 3 examined CSF were positive. The above results indicated that the serological test for detecting the specific IgG antibody by micro-ELISA using paired samples of serum and CSF was very helpful for clinical differentiation of neuro-cysticercosis from neurologic diseases of other causes.

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Heterosis Effects on Jumping Height and Body Weight in Three-Way Rotational Crossing in Mice

  • Kurnianto, E.;Shinjo, A.;Suga, D.;Nakada, T.;Sunagawa, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.10
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    • pp.1353-1358
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    • 2000
  • The three-way rotational crossing experiment has been conducted to evaluate heterosis effects on jumping height and body weight. Yonakuni wild mice (Y) and two genetic groups of $CF_{{\sharp}1}$ (C) and C3H/HeNCrj (H) laboratory mice were used as materials. Reciprocal rotational crossing was made by crossing C male${\times}$Y female and Y male${\times}$C female to produce basic group designated $G_0$ and ${G_0}^{\prime}$, respectively. The females of the $G_0$ and ${G_0}^{\prime}$ were mated to the H sire to produce second generation ($G_1$ and ${G_1}^{\prime}$), and at the following generation the replacement females were mated to Y or C sire according to the basic group to produce $G_2$ to $G_3$ and ${G_2}^{\prime}$ to ${G_3}^{\prime}$. Individual jumping height data at Wk6 and body weight data at 1 (Wk1), 3 (Wk3), 6 (Wk6) and 10 (Wk10) weeks of age were analyzed. The results showed that effects of genetic group, sex and interaction of genetic group by sex were significant (p<0.01) for jumping height. For males, 55.34%~79.17% and 54.46%~78.29% of heterosis were reached at $G_1$ to $G_3$ and ${G_1}^{\prime}$ to ${G_3}^{\prime}$, respectively. While for females at $G_1$ to $G_3$ and at ${G_1}^{\prime}$ to ${G_3}^{\prime}$, heterosis effects were 61.53%~80.42% and 47.79%~85.86%, respectively. For body weight, genetic group was a significant source of variation at all ages studied. Sex effect was significant at Wk3, Wk6 and Wk10, and interaction between genetic group and sex was significant at Wk6 and Wk10 (p<0.01). C sires resulted in the highest body weight of offspring, while H sires were the intermediate and Y sires were the lightest. The significant positive and negative heterosis effects for body weight were exhibited. Crossing involved the Y sires in addition to smaller maternal effects of Y dams tended to result in small heterosis.

Seroprevalence of Dengue Virus Antibody in Korea (한국인에서 뎅기바이러스 항체의 혈청 유병률 연구)

  • Lee, Ji Hyen;Kim, Han Wool;Kim, Kyung-Hyo
    • Pediatric Infection and Vaccine
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    • v.25 no.3
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    • pp.132-140
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    • 2018
  • Purpose: The number of dengue fever cases is rising due to increasing overseas travel. Vaccination makes severe dengue fever in seronegative individuals after vaccination when they exposure to wild-type dengue virus. We investigated the seroepidemiology of the dengue virus for monitoring of Korean dengue virus immunity and establishing the prevention of dengue infection. Methods: The study was based on 446 residual sera collected from 98 infants (2 months to 1 year old), 152 adolescents (13 to 19 years old), 90 adults (20 to 50 years old), and 106 elderly participants (more than 65 years old) for other studies. Antibody levels for dengue virus immunoglobulin G (IgG) in each age group were measured using an enzyme-linked immunosorbent assay (ELISA). For each dengue virus IgG positive or equivocal result, an IgG ELISA was performed for Japanese encephalitis virus. Results: Of the 446 serum samples, only 1 (0.2%) adolescent had a positive result from the dengue IgG antibody test. In the dengue virus IgG antibody test, 14 (3.1%) samples showed equivocal results (10 adolescents and 4 elderly). In the 1 positive case of dengue virus IgG, the Japanese encephalitis IgG test was also positive. In the 14 equivocal cases of dengue virus IgG, there were 6 positive, 3 equivocal, and 5 negative of Japanese encephalitis IgG. Conclusions: The seroprevalence rate of dengue virus was very low in Koreans. This study provides important data for establishing the policy for preventive measures of dengue fever. It will be necessary to continuously monitor for dengue virus immunity.

Relationships Between Growth Characteristics as well as Mineral Consents of Ginseng Seedlings and Yield of Ginseng Roots (묘삼의 생육특성 및 무기성분과 인삼생산과의 관계)

  • 이종철;안대진
    • Journal of Ginseng Research
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    • v.22 no.4
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    • pp.294-298
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    • 1998
  • This study was conducted to investigate the effects of growth characteristics and mineral contents of ginseng seedling grown in different locations on ginseng growth and root yield. Ginseng seedling produced in 16 farmers' field at the semi-Yangjik seedbed were planted and cultivated in a field with same cultural practices. Missing plant was increased with increased year of ginseng age, especially severe at 5-year-old ginseng. Rate of missing plant was different among the origins of the seedling, even with the same weights. Negative correlation was noted between the missing rate of 3-year-old ginseng plants and NH4-N content of the seedling, but positive correlation between the missing rate and K2O and Ca contents of the seedling. Root yield of 6-year-old ginseng was also affected by the origins of ginseng seedlings. Root yield was high in ginseng plant from 0.6∼0.9 g see dings compared to those from over 0.9 g seedlings.

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OPTIMAL RADIOCOLORING OF TREES

  • Zhang, Xiaoling
    • Korean Journal of Mathematics
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    • v.27 no.4
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    • pp.831-841
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    • 2019
  • A Radiocoloring (RC) of a graph G is a function f from the vertex set V (G) to the set of all non-negative integers (labels) such that |f(u) - f(v)| ≥ 2 if d(u, v) = 1 and |f(u) - f(v)| ≥ 1 if d(u, v) = 2. The number of discrete labels and the range of labels used are called order and span, respectively. In this paper, we concentrate on the minimum order span Radiocoloring Problem (RCP) of trees. The optimization version of the minimum order span RCP that tries to find, from all minimum order assignments, one that uses the minimum span. We provide attainable lower and upper bounds for trees. Moreover, a complete characterization of caterpillars (as a subclass of trees) with the minimum order span is given.

The Effects of Failed Airline Services on the Complaint and Switching Behavior (항공서비스 실패가 불평행동과 전환행동에 미치는 영향)

  • Tran, Quang Thai;Kang, Hyunmo;Jeong, Eui Hyeon
    • Knowledge Management Research
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    • v.18 no.2
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    • pp.103-127
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    • 2017
  • This study examines the effects of failed airline services on the consumers' psychological process and their behavioral responses. Having different classifications from the previous studies, this study focuses on dividing important factors of airline services into search attributes(e.g. sale and procedure services, services concerning flight attendant, in-flight services) and experience attributes(e.g. flight services, collateral services). Using structural equation modeling, this study shows that the failure of service factors of the experience attributes provokes the feeling of disappointment with mediation effect of external attribution while the failure of service factors of search attributes provokes the feeling of regret with mediation effect of internal attribution. Finally, disappointment leads to consumers' complaint behavior while regret leads to consumers' switching behavior. Unlike previous studies, we show that when a service failure occurs, depending on each attribute, consumers feel negative emotions of disappointment or regret through different attribution processes and finally show different behavioral responses with an empirical analysis.

Multifunctional Indium Tin Oxide Thin Films

  • Jang, Jin-Nyeong;Yun, Jang-Won;Lee, Seung-Jun;Hong, Mun-Pyo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.186-186
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    • 2015
  • We have introduced multifunctional ITO single thin films formed by normal sputtering system equipped with a plasma limiter which effectively blocks the bombardment of energetic negative oxygen ions. MFSS ITO also possesses high gas diffusion barrier properties simultaneously low resistivity even it deposited at room temperature without post annealing on plastic substrate. Nano-crystalline enhancement by Ar energy has energy window from 20 to 30 eV under blocking NOI condition. Effect of blocking NOI and optimal Ar energy window enhancement facilitate that resistivity is minimized to $3.61{\times}10^{-4}{\Omega}cm$ and the WVTR of 100 nm thick MFSS ITO is $3.9{\times}10^{-3}g/(m^2day)$ which is measured under environmental conditions of 90% relative humidity and 50oC that corresponds to a value of ${\sim}10^{-5}g/(m^2day)$ at room temperature. The multifunctional MFSS ITO with low resistivity, and low gas permeability will be highly valuable for plastic electronics applications.

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Production of Dye-decolorizing Enzyme using Molasses-containing Medium

  • Lee, Tae-Ho;Shoda, Makoto
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.57-58
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    • 2000
  • Production of dye-decolorizing enzyme was investigated with the cultivation of Geotrichum candidum Dec1 (abbreviated to Dec1) using molasses as a cheap raw materials. Molasses was found to stimulate the enzyme production as well as a role of carbon source, because the production increased with molasses content up to 50 g/L. However, the severe inhibition of dye-decolorizing enzyme activity was observed even at low concentration of molasses 10 g/L when purified decolorizing peroxidase was used. Its inhibitory effect was reduced through the cultivation of Dec1. The fractions of molasses separated by a gel chromatography showed the different degrees of inhibition. As a way to reduce the inhibitory effect, the dilution of culture broth was examined, and the total decolorizing activity for Reactive blue 5 increased 7 times as much as that of original culture broth by 30 times dilution. On the basis of result, we proposed a process scheme which can fully utilize both positive and negative effect of molasses in dye-decolorizing process using molasses.

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Fabrication of High-Density Nickel Hydroxide Powder-I (고밀도 수산화니켈 분말의 제조에 관한 연구-I)

  • 신동엽;조원일;신치범;조병원;강탁;윤경석
    • Journal of the Korean institute of surface engineering
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    • v.28 no.2
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    • pp.92-100
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    • 1995
  • To increase the capacity of positive electrode materials for matching the high capacity negative electrode materials in alkaline rechargeable batteries, high-density nickel hydroxide powders were made through a continuous process from nickel sulfate reacted with ammonia and sodium hydroxidc. The effect of operating conditions on structure, shape, size distribution, apparent density and tap density of powders were investigated. Crystal structure of nickel hydroxide powder was hcp according to Bravais Lattice. The increase of mean residence time promoted the growth of (101) plane. The shape of powder was nearly spherical. Their size was in the range of $2~50\mu\textrm{m}$. The size distribution of the powders prepared was narrower than that of commercially obtained nickel hydroxide. Apparent density and tap density were 1.6~1.7g/cc and 2.0~2.1g/cc, respectively.

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Capacitance-Voltage (C-V) Characteristics of Cu/n-type InP Schottky Diodes

  • Kim, Hogyoung
    • Transactions on Electrical and Electronic Materials
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    • v.17 no.5
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    • pp.293-296
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    • 2016
  • Using capacitance-voltage (C-V) and conductance-voltage (G/ω-V) measurements, the electrical properties of Cu/n-InP Schottky diodes were investigated. The values of C and G/ω were found to decrease with increasing frequency. The presence of interface states might cause excess capacitance, leading to frequency dispersion. The negative capacitance was observed under a forward bias voltage, which may be due to contact injection, interface states or minority-carrier injection. The barrier heights from C-V measurements were found to depend on the frequency. In particular, the barrier height at 200 kHz was found to be 0.65 eV, which was similar to the flat band barrier height of 0.66 eV.