• Safety and Health at Work
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• 제11권4호
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• pp.479-484
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• 2020

Background: The purpose of this study is to examine the combined effects of organizational climate (OC) with emotional labor (EL) on turnover intention in Korean firefighters. Methods: The data were obtained from the study Firefighters Research: Enhancement of Safety and Health. A total of 4,860 firefighters whose main duty was providing "emergency medical aid" were included. To examine the effects of OC on the relationships between five subscales of EL and turnover intention, four groups were created using various combinations of OC ("good" vs. "bad") and EL ("normal" vs. "risk"): (1) "good" and "normal" (Group I), (2) "bad" and "normal" (Group II), (3) "good" and "risk" (Group III), and (4) "bad" and "risk" (Group IV). Multivariate logistic regression analyses were performed to estimate the risk of turnover intention for the combinations of OC and EL. Results: The results showed turnover intention was significantly higher in the group with "bad" OC (17.7%) than in that with "good" OC (7.6%). Combined effects of OC and EL on turnover intention were found in all five subscales with the exception of Group I for emotional demands and regulation. Groups II, III, and IV were more likely to experience risks of turnover intention than Group I (p for trend <0.001). Conclusions: A positive and cooperative OC plays a role in decreasing the risk of turnover intention and in attenuating the negative effects of EL on turnover intention in firefighters.

• 대한본초학회지
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• 제33권4호
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• pp.35-41
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• 2018

Objectives : Hyeonggaeyeongyotang Gagambang (HYT) is a herbal medicine prescribed for the treatment of inflammatory diseases, but it is necessary to study the exact therapeutic efficacy. This study aims to investigate the antibacterial and anti-inflmmatory activities of HYT. Methods : Antibacterial activity of HYT was confirmed by staining Escherichia coli, a gram negative strain, and Staphylococcus aureus, a gram positive strain, on solid Lysogeny Broth (LB) medium containing HYT. Antioxidant activity of HYT was confirmed by 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha ($I{\kappa}B{\alpha}$) after lipopolysaccharide (LPS) treatment with HYT-treated RAW 264.7 mouse macrophages cells was confirmed by immunoblot analysis and the level of interleukin 1 beta (IL-$1{\beta}$) mRNA expression level was confirmed by quantitative real-time PCR. Results : HYT showed a concentration-dependent antibacterial activity against Escherichia coli and Staphylococcus aureus and also showed excellent antioxidant activity. HYT treatment attenuated the phosphorylation of $I{\kappa}B{\alpha}$induced by LPS treatment in RAW 264.7 mouse macrophages cells. The phosphorylation of $I{\kappa}B{\alpha}$is crucial for the regulation of the expression of various pro-inflammatory cytokines. In addition, IL-$1{\beta}$ mRNA expression level of RAW 264.7 mouse macrophages cells stimulated by LPS treatment was also inhibited by HYT treatment. Conclusions : Through experimental demonstration of the antioxidative, antimicrobial and anti-inflammatory effects of HYT, we demonstrated that HYT is a herbal medicine effective for the treatment of inflammatory diseases caused by various bacterial infections.

• 한국인터넷방송통신학회논문지
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• 제22권3호
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• pp.151-161
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• 2022

글로벌 CP들로 인해 인터넷의 트래픽이 급증하면서 망 투자비용을 분담하여야 한다는 주장이 업계에서 대두하였다. 이로 인해 망 중립성의 원칙이 접근성에서 비용 부담으로 논점이 크게 바뀌었다. 유료화된 트래픽의 우선처리가 각국에서 인정을 받았음에도 불구하고, 학계의 일부에서는 인터넷 공간에서 망 사용료 지불에 대한 부정적인 시각이 있었다. 그러나 업계에서는 사업자들 사이에 분쟁이 다수 발생하였고, 일부는 법정 다툼으로 비화되면서 법원의 판결에 관심이 쏠렸다. 법원이 망 중립성 하에서 유상성을 인정하기 시작하였고, 정부도 이에 대응하여 규제를 정비하는 등 우리나라는 빠르게 대처해 왔다. 그러나 여전히 서비스 안정에 초점을 맞추고 있으며, 직접적으로 망 이용대가의 지불을 규정한 규제는 아직 없다. 본 연구에서는 망 중립성에 대한 사업자간 분쟁 및 법원의 판결, 규제의 개선 사례를 분석하여 망 중립성의 변화를 고찰하였으며, 공유지의 비극 사례를 참고하여 수익자 부담의 원칙에 입각한 올바른 가격신호의 복원이 가장 중요한 해결책임을 제시하였다. CP의 망 사용료 지급도 그 방안의 하나이다.

• 해양환경안전학회지
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• 제29권1호
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• pp.15-23
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• 2023

정부는 행정규제기본법 개정('19.07.17)을 통하여 경제활력 제고를 위하여 규제를 최소화하고 포괄적 네거티브 규제원칙을 적용하였다. 사전규제에서 우선허용-사후규제로의 정책환경이 변화하였다. 하지만 해양오염방제 자재·약제는 법적의무인증제도가 적용되어 제품을 판매할 때마다 검정을 실시하여 제조업체의 자율성을 억제하여 왔다. 또한 방제자재·약제 생산업체는 대부분 영세하여 구입자가 주문 의뢰 시마다 제품을 소량 생산하는 방식을 취하고 있다. 이에 해양오염방제 자재·약제 형식승인, 성능시험, 검정제도도 시대의 흐름에 맞춰 시장 및 업계의 자율성을 보장하고 효율성 향상을 위해 규제완화의 필요성이 제기되었다. 본 연구에서는 현 제도의 문제점을 파악하고 국내·외 제도를 비교·분석을 통한 개선방안을 제안하였다

• 예술인문사회 융합 멀티미디어 논문지
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• 제7권3호
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• pp.401-410
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• 2017

증강현실 게임은 현실 공간에서 발생 가능한 물리적 위험성을 내포하고 있다는 점에서 기존의 게임과는 차이가 있다. 실제로 포켓몬 고 게임과 관련한 사고가 증가하고 있는 추세임에도 이러한 유형의 위험에 대한 학술적 논의는 부족한 상황이다. 따라서 본 연구에서는 증강현실 게임에서 나타나는 인지된 위험 유형에 대한 탐색과 함께 위험 인식 수준이 이용의도에 미치는 영향을 검증한다. 또한 일반적으로 게임 중독성향 및 감각추구 성향이 강하게 나타나는 청소년 그룹과 청장년층의 인식 수준 비교를 통해 연구 시사점을 도출한다. 연구 결과에 따르면 10대의 경우 경제적 위험 인식이 증가할수록 이용 의도가 감소하고 20대 이상의 경우 시간적 위험 인식이 높을수록 이용 의도가 낮아지는 것으로 나타났다. 물리적/신체적 위험의 경우 10대의 경우 이용 의도 감소에 유의미한 영향을 미치지 않는 것으로 나타났고 20대 이상의 경우 오히려 정의 관계가 있는 것으로 나타났다. 이러한 결과는 향후 증강현실 게임의 안전한 이용을 위해서 적절한 규제의 필요성을 시사한다.

• 인간식물환경학회지
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• 제22권6호
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• pp.611-619
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• 2019

This study was conducted to investigate changes in the mental functions of the elderly in nursing facilities affected by a horticultural therapy program based on validation therapy. To meet the purpose, we conducted a horticultural therapy program based on validation therapy with 58 elderly participants (average age 79.12±6.84, men and women) once a week, 50 minutes per session, in a total of 10 sessions. Participants were divided into the control and experimental group with convenience sampling. The program was carried out from March 15 to November 22, 2018. Vibraimage 8 pro(ELSYS, 2014) is a recent, psychologically based, emotional-recognition visual imaging technology that measures pixels microvibration in terms of digital frequency and amplitude parameters. To examine the effects of the horticultural therapy program based on validation therapy, Vibraimage was used to assess aggression, stress, tension, suspect, balance, charm, energy, self-regulation, inhibition, neuroticism and positive, negative, physiological domains of mental functions before and after program. As a result, the mean score of the positive domain in the control group significantly decreased from 63.89±5.09 to 60.74±5.48, but it decreased without statistical significance in the experimental group from 63.98±5.45 to 61.39±6.02. The mean score of neuroticism in the experimental group significantly decreased from 31.64±10.94 to 22.87±13.79. Moreover, the mean score of the physiological domain in the experimental group also significantly decreased from 25.08±6.27 to 19.42±8.80. Accordingly, horticultural therapy program based on validation therapy can be utilized as a program to promote mental health, especially maintaining positive mental health function of the eldery, helping those who live in long-term care facilities enjoy a happier life more happier.

• 한국심리학회지:학교
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• 제8권3호
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• pp.379-403
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• 2011

본 연구는 재한 중국인 유학생들의 문화적응과정에서 그들의 사용하는 대처전략과 스스로 지각하는 변화양상에 대해 알아보았다. 한국에 거주하고 있는 56명의 중국유학생들이 연구에 참여하고, 개념도(concept mapping)를 이용하여 자료를 분석하였다. 그 결과 중국인 유학생들은 '언어적 유능감 향상'과 '사회적 네트워크 활용'을 중요한 대처전략으로 생각하며, '문화적 자기', '개인적 자기'의 변화를 포함하여 광범위한 측면에서의 변화를 인식하고 있음을 확인하였다. 대처전략과 변화양상에 대한 인식은 체류기간에 따라 차이를 보였는데, 장기 체류한 중국인 유학생들은 단기 유학생과는 달리 '기분조절'을 대처전략으로 삼았다. 또한, 단기 유학생들이 긍정적 변화양상을 우세하게 보고하는 반면,장기 유학생들은 긍정적 변화보다는 부정적 변화를 더 인식하는 것으로 나타났다. 이 연구 결과는 추후 대학 관계자들이 체류기간에 따라 다른 경험을 하게 되는 중국 유학생을 이해하고 민감하게 대응할 필요가 있음을 시사한다.

• 치위생과학회지
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• 제23권4호
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• pp.296-301
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• 2023

Background: Periodontal disease is a major cause of tooth loss in adults and is a representative oral disease commonly suffered by most people around the world. Mainly the proliferation of Gram-negative bacteria and secreted virulence factors cause an inflammatory response and destroy periodontal tissue. Gossypetin, isolated from Hibiscus sabdariffa L, is known to have various pharmacological effects, including antibacterial and anticancer activities. We aimed to confirm the anti-inflammatory effect of gossypetin through interleukin-6 (IL-6) regulation in human gingival fibroblasts (HGFs) stimulated with lipopolysaccharide (LPS) of Porphyromonas gingivalis, a major cause of adult periodontitis. Methods: CCK-8 assay was performed to confirm the concentration-dependent cytotoxicity of gossypetin against HGFs. The secretion level and mRNA expression of IL-6, an inflammation-related cytokine, and the effect of gossypetin on these in HGFs stimulated with P. gingivalis LPS were confirmed by ELISA and qRT-PCR analysis, respectively. Results: Up to a concentration of 100 µM gossypetin with or without P. gingivalis LPS, the survival rate for HGFs was maintained at over 95% and showed no toxicity. ELISA and qRT-PCR analysis results showed that P. gingivalis LPS increased IL-6 secretion and mRNA levels in HGFs compared to the control group. However, this increase in IL-6 was significantly down-regulated by gossypetin treatment in a dose-dependent manner. In particular, 80 µM gossypetin inhibited IL-6 production to the level of the control group. Conclusion: These results indicated that gossypetin attenuated IL-6 production in HGFs stimulated by P. gingivalis LPS, which may ultimately suppress the inflammatory response in periodontal tissue. Therefore, gossypetin may have potential as a natural ingredient for the prevention and treatment of periodontal disease.

• International Journal of Molecular Medicine
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• 제44권2호
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• pp.491-502
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• 2019

Although the migration of hepatic stellate cells (HSCs) is important for hepatic fibrosis, the regulation of this migration is poorly understood. Notably, transforming growth factor (TGF)-β1 induces monocyte migration to sites of injury or inflammation during the early phase, but inhibits cell migration during the late phase. In the present study, the role of transforming protein RhoA signaling in TGF-β1-induced HSC migration was investigated. TGF-β1 was found to increase the protein and mRNA levels of smooth muscle actin and collagen type I in HSC-T6 cells. The level of RhoA-GTP in TGF-β1-stimulated cells was significantly higher than that in control cells. Furthermore, the phosphorylation of cofilin and formation of filamentous actin (F-actin) were more marked in TGF-β1-stimulated cells than in control cells. Additionally, TGF-β1 induced the activation of nuclear factor-κB, and the expression of extracellular matrix proteins and several cytokines in HSC-T6 cells. The active form of Rap1 (Rap1 V12) suppressed RhoA-GTP levels, whereas the dominant-negative form of Rap1 (Rap1 N17) augmented RhoA-GTP levels. Therefore, the data confirmed that Rap1 regulated the activation of RhoA in TGF-β1-stimulated HSC-T6 cells. These findings suggest that TGF-β1 regulates Rap1, resulting in the suppression of RhoA, activation of and formation of F-actin during the migration of HSCs.

• 식물병연구
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• 제30권3호
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• pp.268-277
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• 2024

Erwinia pyrifoliae is a gram-negative bacterial pathogen that commonly causes black shoot blight in pear and apple tree. Although the pathogenicity of this bacterial species is very similar to E. amylovora, there is no specific explanation of its pathogenic genes and mechanisms. In this study, our investigation into E. pyrifoliae pathogenicity involved generating seven YKB12327 mutant strains using Tn5 transposon mutagenesis. Observations revealed weakened growth rate and loss of pathogenicity in these mutants. Whole-genome sequencing and alignment analysis identified transposon insertions within the coding sequences of five strains and in the intergenic region of two strains. Annotation analysis elucidated genes directly or indirectly associated with pathogenicity. Notably, mutant strain MT16 displayed a transposon insertion mutation in the cyclic-di-GMP phosphodiesterase (pdeF) gene, a key player in bacterial signaling, governing microbial behavior and adaptation to environmental changes. Our findings provide insights into the genetic regulation of E. pyrifoliae pathogenicity, suggesting potential avenues for further research aimed at understanding and controlling this bacterial pathogen by targeting pdeF to mitigate apple black shoot blight disease.