• Journal of Microbiology and Biotechnology
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• v.6 no.3
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• pp.219-220
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• 1996

The pKH2 isolated from the multidrug-resistant Staphylococcus aureus SA2 is a 40.98-kb plasmid and mediates resistance to ampicillin, clindamycin, erythromycin, kanamycin, and streptomycin. The 3.4-kb HindIII fragment conferring kanamycin resistance was cloned from the pKH2 into pBluescriptII $KS^+$ and partial sequence determination of that fragment was carried out. Sequence analysis revealed that the kanamycin resistance gene which encoded aminoglycoside 3'-phosphotransferase was linked to the streptothricin resistance gene. But a nonsense mutation was found in the streptothricin resistance gene and this mutation resulted in a truncated protein of streptothricin acetyltransferase. Homology comparison with nucleotide sequence databases revealed that the 3.4-kb HindIII fragment of pKH2 had been derived not from S. aureus but from Gram-negative Campylobacter coli.

• Journal of the Chungcheong Mathematical Society
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• v.31 no.4
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• pp.487-509
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• 2018

The Truncated Moment Problem (TMP) entails finding a positive Borel measure to represent all moments in a finite sequence as an integral; once the sequence admits one or more such measures, it is known that at least one of the measures must be finitely atomic with positive densities (equivalently, a linear combination of Dirac point masses with positive coefficients). On the contrary, there are more general moment problems for which we aim to find a "signed" measure to represent a sequence; that is, the measure may have some negative densities. This type of problem is referred to as the General Truncated Moment Problem (GTMP). The Jordan Decomposition Theorem states that any (signed) measure can be written as a difference of two positive measures, and hence, in the view of this theorem, we are able to apply results for TMP to study GTMP. In this note we observe differences between TMP and GTMP; for example, we cannot have an analogous to the Flat Extension Theorem for GTMP. We then present concrete solutions to lower-degree problems.

• Proceedings of the KIPE Conference
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• 2015.07a
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• pp.177-178
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• 2015

This paper presents a neutral point deviation compensating control algorithm applied to a 3-level NPC converter. The neutral point deviation is analyzed with a focus on the current flowing out of or into the neutral point of the dc link. Based on the zero sequence components of the reference voltages, this paper analyzes the neutral point deviation and balancing control for 3-level NPC converter. An analytical method is proposed to calculate the injected zero sequence voltage for NP balancing based on average neutral current. This paper also proposes a control scheme compensating for the neutral point deviation under generalized unbalanced grid operating conditions. The positive and negative sequence components of the pole voltages and ac input currents are employed to accurately explain the behavior of 3-level NPC converter. Simulation and experimental results for a test set up of 30kW are shown to verify the validity of the proposed algorithm.

• Bulletin of the Korean Chemical Society
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• v.21 no.11
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• pp.1077-1084
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• 2000

The dipolar effect of neighboring protons that are not directly bonded to the carbon of interest on coupled carbon-13 relaxation in a simple organic molecule has been studied by comparing the relaxation behaviors of labeled carbon-13 in $Br13CH_2COOH$ with those in $BrCH_213COOH.$ Various pulse sequences, such as coupled inversion recovery pulse sequence, J-negative and J-positive pulse sequence, and nonselective and selective proton ${\pi}pulse$ sequence, were employed to perform the required coupled spin relaxation experiments. To gain information on various spectral densities, including that of dipolar-CSA cross correlation, the experiments were performed on two different spectrometers, operating, respectively, at 50.31 and 125.51MHz for 13C. The magnitude of CH dipolar spectral densities for $BrCH_213COOH$ was found to be about 8% of those for $Br13CH_2COOH$, which means the effect due to the protons not directly bonded to the carbon of interest is small but not completely negligible.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.63 no.10
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• pp.1364-1370
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• 2014

Power system fault analysis has been based on symmetrical component method, which describes power system elements by positive, negative and zero sequence impedance. Obtaining accurate line impedances as possible are very important for estimating fault current magnitude and setting distance relay accurately. Especially, accurate calculation of zero sequence impedance is important because most of transmission line faults are line-to-ground faults, not balanced three-phase fault. Since KEPCO has started measuring of transmission line impedance at 2005, it has been revealed that the measured and calculated line impedances are well agreed within reasonable accuracy. In case of underground transmission lines, however, large discrepancies in zero sequence impedance were observed occasionally. Since zero sequence impedance is an important input data for distance relay to locate faulted point correctly, it is urgently required to analyze, detect and consider countermeasures to the source of these discrepancies. In this paper, development of pre/post processor to ATP (Alternative Transient Program) version of EMTP (Electro-Magnetic Transient Program) for sequence impedance calculation was described. With the developed processor ATP-cable, effects of ground resistance and ECC (Earth Continuity Conductor) on sequence impedance were analyzed.

• Honam Mathematical Journal
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• v.29 no.3
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• pp.367-375
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• 2007

For bounded negatively associated random variables we derive almost sure convergence and specify the associated rate of convergence by establishing exponential inequality.

• Interdisciplinary Bio Central
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• v.6 no.4
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• pp.3.1-3.10
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• 2014

Amino acid substitution matrices are essential tools for protein sequence analysis, homology sequence search in protein databases and multiple sequence alignment. The PAM matrix was the first widely used amino acid substitution matrix. The BLOSUM series then succeeded the PAM matrix. Most substitution matrixes were developed by using the statistical frequency of substitution between each amino acid at blocks representing groups of protein families or related proteins. However, substitution of amino acids is based on the similarity of physiochemical properties of each amino acid. In this study, a new approach was used to obtain major physiochemical properties in multiple sequence alignment. Frequency of amino acid substitution in multiple sequence alignment database and selected attributes of amino acids in physiochemical properties database were merged. This merged data showed the major physiochemical properties through principle components analysis. Using factor analysis, these four principle components were interpreted as flexibility of electronic movement, polarity, negative charge and structural flexibility. Applying these four components, BAPS was constructed and validated for accuracy. When comparing receiver operated characteristic ($ROC_{50}$) values, BAPS scored slightly lower than BLOSUM and PAM. However, when evaluating for accuracy by comparing results from multiple sequence alignment with the structural alignment results of two test data sets with known three-dimensional structure in the homologous structure alignment database, the result of the test for BAPS was comparatively equivalent or better than results for prior matrices including PAM, Gonnet, Identity and Genetic code matrix.

• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.457-462
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• 2003

The complete nucleotide sequence of a plasmid, pMG1, isolated from Bifidobacterium longum MG1 has been determined. This plasmid, composed of 3,862 base pairs with 65.1% of G+C content. harbors two major open reading frames (ORF) encoding putative proteins of 29 kDa (ORF I) and 71 kDa (ORF II). ORF I showed relatively high amino acid sequence homology with replication proteins of other plasmids from Gr Im-positive and -negative bacteria. Upstream of ORF I, four sets of tandem repeat sequences resembling the iteron structure of related plasmids were found. S1 endonuclease treatment and Southern blot analysis revealed that pMG1 accumulates single-stranded DNA (ssDNA) intermediate, which indicate i the rolling circle replication (RCR) mechanism of this plasmid. Homology search indicated that ORF II encodes plasmid mobilization protein, and the presence of highly conserved oriT sequence in the upstream of this gene supported this assumption. RT-PCR showed that only ORF I is expressed in vivo. Based on these results, pMG 1 was exploited to construct a shuttle vector, pBES2. It was successfully transformed into Bifidobacterium and maintained stably.

• Journal of applied mathematics & informatics
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• v.30 no.3_4
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• pp.623-633
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• 2012

Let {${\Omega}$, $\mathcal{F}$, P} be a probability space and {$X_n|n{\geq}1$} be a sequence of random variables defined on it. A finite sequence of random variables {$X_n|n{\geq}1$} is said to be conditionally negatively associated given $\mathcal{F}$ if for every pair of disjoint subsets A and B of {1, 2, ${\cdots}$, n}, $Cov^{\mathcal{F}}(f_1(X_i,i{\in}A),\;f_2(X_j,j{\in}B)){\leq}0$ a.s. whenever $f_1$ and $f_2$ are coordinatewise nondecreasing functions. We extend the H$\grave{a}$jek-R$\grave{e}$nyi-type inequality from negative association to conditional negative association of random variables. In addition, some corollaries are given.

• Journal of Power Electronics
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• v.16 no.4
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• pp.1551-1564
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• 2016

The high penetration level of inverter-based distributed generation (DG) power plants is challenging the low-voltage ride-through requirements, especially under unbalanced voltage sags. Recently, a flexible injection of both positive- (PS) and negative-sequence (NS) reactive currents has been suggested for the next generation of grid codes. This can enhance the ancillary services for voltage support at the point of common coupling (PCC). In light of this, considering distant grid faults that occur in a mainly inductive grid, this paper proposes a complete voltage support control scheme for the interface inverters of medium or high-rated DG power plants. The first contribution is the development of a reactive current reference generator combining PS and NS, with a feature to increase the PS voltage and simultaneously decrease the NS voltage, to mitigate voltage imbalance. The second contribution is the design of a voltage support control loop with two flexible PCC voltage set points, which can ensure continuous operation within the limits required in grid codes. In addition, a current saturation strategy is also considered for deep voltage sags to avoid overcurrent protection. Finally, simulation and experimental results are presented to validate the effectiveness of the proposed control scheme.