• Journal of Embryo Transfer
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• 제14권1호
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• pp.1-8
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• 1999

The efficiency of transgenic livestock animal production may be improved by early selection of transgenci preimplantation embryos. To examine the possibility of GFP gene as a non-invasive marker for the early screening of transgenic embryo, the GFP gene was microinjected into rabbit zygotes and the later stages of preimplantation embryos were examined for the expression of GFP. The presence of injected DNA was detected by PCR analysis and the expression of GFP was detected by observing green fluorescence in embryos under a fluorescent microscope. Out of 108 GFP gene-injected rabbit zygotes, seventy three(67.6%) were fluorescence-positive. When 11 fluroresecence-positive blastocysts were analyzed for the presence of GFP gene by PCR, 6(54.5%) were positive, and all of the 8 flrouescence-negative blastocysts were also negative by PCR. The results indicate that the screening of transgene in rabbit embryos by PCR analysis and GFP detection could be a promising method for the preselection of transgenic embryos.

• Proceedings of the Technology Innovation Conference
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• 기술경영경제학회 1997년도 제11회 하계학술발표회 논문집
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• pp.57-82
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• 1997

This paper empirically examines various personal characteristics and preferences of HRM (Human Resorce Mamagement) systems using data from 968 R&D professionals in 9 private R&D organizations. It identifies different patterns of reward and career development needs associated with 4 types of R&D professionals, who are categorized by personal characteristics: personality and attitude. A productive leader type shows active personal traits (which encompass high scores of need for achievement, risk-taking propensity, tolerance for ambiguity, and self-efficacy) and positive attitudes (which include high scores of job satisfaction, organizational commitment, and low scores of turnover intention), while a cynical bystander type exhibits passive personal traits and negative attitudes toward a job. A passive adapter, who has passive personal characteristics but positive attitudes, and a frustrated champion, who is active in personality but negative in current job attitude, lie in between them. The results reveal that: 1) These four different types of R&D professionals show differences in their education, age and tenure level, and technical performance. 2) They have different career orientations and problem solving styles. 3) They also exhibit different preferences in performance appraisal criteria and rater, and reward structures. Based on these findings, this study suggests a contingent type of human resource management in terms of selection and staffing of R&D professionals, performance appraisal, incentive systems, and career development plans.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.872-880
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• 2003

Three vectors, pSG1, 2, and 3, which facilitate in vivo expression technology (IVET) in Gram-negative bacteria, were developed. Vectors pSG1and 2 are derivatives of ColE1, and pSG3 is a derivative of an R6K replicon. These vectors contain oriT sites that allow mobilization when the RK2 Tra functions are provided in trans. These vectors contain promoterless lacZ (pl-lacZ) and promoterless aph (pl-aph) transcriptionally fused together, which allow qualitative and quantitative measurements of the expression of genes in the genome of bacterial cells. pSG1 and 3 contain gentamicin-resistance genes, and pSG2 carries a streptomycin-/spectinomycin-resistance gene, allowing for selection of recombinants generated by a single crossover between a library fragment cloned into a pSG vector and the identical region in the genome of a bacterial species from which the library fragment originated. These vectors were successfully applied to the generation of random fusions at high rates in the genomes of four representative Gram-negative bacteria. In addition, the expression level of ${\beta}-galactosidase$ and the degree of resistance to kanamycin in cells with fusions generated by these vectors were found to be linearly correlated, proving that these vectors can be used for IVET.

• Knowledge Management Research
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• 제12권3호
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• pp.1-25
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• 2011

This research empirically examined the determinants of competitive advantages and performance of Korean film industry from 1996 to 2003 over a sample of 396 movies. Especially, the effects of knowledge-based resources, social capital, product differentiation strategy, and market entry strategy on a film performance were hypothesized and empirically tested. Findings of this study are as follows; First, confirming the importance of knowledge-based resources in film industry, both the superior business track records of producers and the superior artistic track records of directors and actors had positive effects on film performance. Second, supporting social capital hypotheses, while the strong ties between directors and producers had positive effects, the strong ties between directors and actors had negative effects on film performance. Third, confirming the importance of product differentiation strategy, genre differentiation against competing films had positive effects on film performance. Finally, confirming the importance of market entry timing, the number of competing films released following a film opening within a week had negative effects on film performance. The findings of this research suggest that producers, directors, and actors are not only the critical knowledge-based resources to create competitive advantages indeed, but the pattern of relationships among them also systematically influences the film performance. Moreover, it is suggested that film performance may be improved by the proper genre differentiation and opening day selection.

• International Journal of Industrial Entomology and Biomaterials
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• 제26권1호
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• pp.13-21
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• 2013

Boil-off loss ratio is one of the most important economic traits to be considered during the course of silkworm breeding. The boil-off loss ratio varies among the breeds and seasons. The present study was undertaken with 5 oval and 5 peanut breeds and twenty five hybrids involving these parents to select the promising hybrids with desired boil-off loss ratio. Accordingly, studies were undertaken to estimate the heterosis and heterobeltiosis. It was observed that majority of the hybrids revealed negative heterosis for boil-off loss ratio which is desirable. Based on the heterosis and heterobeltiosis, among all the hybrids, the hybrid APBRO5 ${\times}$ APBRD5 exhibited highest negative heterosis for boil-off loss ratio and also exhibited highest EI value (64.5) for the quantitative and qualitative traits. Based on the hybrids performance, boil-off loss ratio, heterosis and heterobeltiosis and evaluation index, the hybrids viz. APBRO5 ${\times}$ APBRD5, APBRO1 ${\times}$ APBRD1 and APBRO3 ${\times}$ APBRD4 were identified for commercial exploitation.

• Korean Journal of Child Studies
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• 제8권1호
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• pp.49-64
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• 1987

The purpose of this study was to investigate the effect of information of appearance, peer preference, and personality traits on children's attraction to unacquainted same sex peers, and to determine the relative importance of the three types of information for the selection of a friend. The subjects of this study were 160 (80 boys and 80 girls) second grade children in Seoul. A Preference Questionnaire was administered in Session I to determine the likes of each subjects. The modified Preference Questionnaire (Reaves, 1981) was used. The subjects were then randomly assigned to one of eight experimental conditions. In Session II, subjects were shown pictures of attractive and unacttractive children, described as having a positive or negative character, with preference similar Of dissimilar to their own. Then they were administered the Interpersonal Attraction Scale (Reaves, 1981). The data were analyzed by three-way ANOVA, $Scheff\acute{e}$ test, and $X^2$. The results of this study were as a follows: The physically attractive stimulus child was preferred to the physically unattractive stimulus child. The stimulus child with preferences similar to the subjects' was preferred to the stimulus with dissimilar preferences. The stimulus with positive personality traits was preferred to the stimulus ehild with a negative personality. A difference in order of importance among the variables with regard to their influence on attraction ratings was found. Personality had stronger influence on attraction ratings than appearance or preference.

• Steel and Composite Structures
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• 제18권3호
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• pp.659-672
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• 2015

This research deals with the analytical solution of a curved beam with different shapes made of functionally graded materials (FGM's). It was assumed that modulus of elasticity is graded along the thickness direction of curved beam based on a power function. The beam was loaded under pure bending. Using the linear theory of elasticity, the general relation for radial distribution of radial and circumferential stresses of arbitrary cross section was derived. The effect of nonhomogeneity was considered on the radial distribution of circumferential stress. This behavior can be investigated for positive and negative values of nonhomogeneity index. The novelty of this study is application of the obtained results for different combination of material properties and cross sections. Achieved results indicate that employing different nonhomogeneity index and selection of various types of cross sections (rectangular, triangular or circular) can control the distribution of radial and circumferential stresses as designer want and propose new solutions by these options. Increasing the nonhomogeneity index for positive or negative values of nonhomogeneity index and for various cross sections presents different behaviors along the thickness direction. In order to validate the present research, the results of this research can be compared with previous result for reachable cross sections and non homogeneity index.

• Research in Community and Public Health Nursing
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• 제20권3호
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• pp.316-326
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• 2009

Purpose: The purpose of this study was to examine teenagers' Internet addiction and to find factors influencing it. Methods: Data were collected from 596 middle and high school students between June 1 and June 20, 2009 with a self-rating questionnaire. The data were processed with SPSS/WIN 12.0. Results: (1) 20.3% of the students were spending over three hours a day in Internet use. The percentages of mild and serious Internet addiction were 22.3 and 2.1, respectively. (2) The stronger Internet addiction was, the higher stress was and the lower social support was. There was a negative correlation between stress and social support (r=-.296, p<.01). There was also a positive correlation between Internet addiction and stress (r=.264, p<.01) while a negative correlation between Internet addiction and social support (r=-.127, p<.01). (3) Among the subjects' characteristics, sex, school and family type, frequency of processed food intake, average daily Internet use hour and sleeping hour, subjective health perception, and satisfaction level with parents and family made difference in the Internet addiction level. Conclusion: The result of this study will provide useful background data for the selection of target population, stress management and social support promotion programs to prevent teenagers' Internet addiction.

• Communications for Statistical Applications and Methods
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• 제23권3호
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• pp.203-213
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• 2016

We investigate volatility spillover aspects of realized volatilities (RVs) for the log returns of the Korea Composite Stock Price Index (KOSPI) and the Hang Seng Index (HSI) from 2009-2013. For all RVs, significant long memories and asymmetries are identified. For a model selection, we consider three commonly used time series models as well as three models that incorporate long memory and asymmetry. Taking into account of goodness-of-fit and forecasting ability, Leverage heteroskedastic autoregressive realized volatility (LHAR) model is selected for the given data. The LHAR model finds significant decompositions of the spillover effect from the HSI to the KOSPI into moderate negative daily spillover, positive weekly spillover and positive monthly spillover, and from the KOSPI to the HSI into substantial negative weekly spillover and positive monthly spillover. An interesting result from the analysis is that the daily volatility spillover from the HSI to the KOSPI is significant versus the insignificant daily volatility spillover of the KOSPI to HSI. The daily volatility in Hong Kong affects next day volatility in Korea but the daily volatility in Korea does not affect next day volatility in Hong Kong.

• Journal of Power Electronics
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• 제19권1호
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• pp.89-98
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• 2019

This paper analyzes the input side performance of a conventional three-phase to single-phase matrix converter (3-1MC). It also presents the input-side waveform quality under this topology. The suppression of low-frequency input current harmonics is studied using the 3-1MC plus capacitance compensation unit. The constraint between the modulation function of the output and compensation sides is analyzed, and the relations among the voltage utilization ratio and the output compensation capacitance, filter capacitors and other system parameters are deduced. For a 3-1MC without large-capacity energy storage, the system performance is susceptible to input voltage imbalance. This paper decouples the inner current of the 3-1MC using a Lyapunov function in the input positive and negative sequence bi-coordinate axes. Meanwhile, the outer loop adopts a voltage-weighted synthesis of the output and compensation sides as a cascade of control objects. Experiments show that this strategy suppresses the low-frequency input current harmonics caused by input voltage imbalance, and ensures that the system maintains good static and dynamic performances under input-unbalanced conditions. At the same time, the parameter selection and debugging methods are simple.