• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.80-80
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• 1993

(목적) 본 연구에서 사용된 바이러스는 HIV-1 nef유전자가 일부 삭제되고 대신 Chloramphenicol acetyltransferase(CAT)가 pSVCAT recombinant 바이러스다. 이러한 recombinant 바이러스를 사용하는 이유는 첫째, CAT activity가 매우 민감하므로 바이러스의 복제억제 정도를 정확하게 측정 할 수 있고 둘째, simian immunodeficiency virus(SIV)의 경우 nef 유전가 in vivo에서는 바이러스의 복제에 필수적이므로 HIV가 SIV와 유사한 것으로 미루어 본 연구에서 사용되는 recombinant SVCAT 바이러스가 안전한 것으로 고려되기 때문이다. (방법) 특히 화합물이 HIV-1의 복제에 얼마나 영향이 있는가는 1) 어느정 도의 virus inoculm을 넣었는지 2) 사용하는 cell line 3) 사용한 cell line의 infection kinetics 4) 실험의 지속기간 5) 테스트하는 assay의 sensitivity에 의존한다. 따라서 $10^{5}$ cell의 H9과 sup T1을 24 well plate에 넣고 sup T1 cell line의 경우 3일 후 항 화합물에 의한 syncytia 형성 및 CAT activity의 억제정도를 현재 AIDS drug으로 쓰이고 있는 Zidovudine을 control로 비교 관찰하였다. H9 cell line의 경우 3일 간격으로 media의 3/4을 fresh media로 바꾸어 주고 9일 후 CAT assay를 하였다. 이러한 assay에서 activity를 보이는 화합물을 reverse transcriptase와 P24 ELISA assay를 재확인하였다.다.

• Proceedings of the Microbiological Society of Korea Conference
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• 1998.04a
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• pp.42.1-42.1
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• 1998

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.239.3-240
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• 2000

No Abstract, See Full Text

• Proceedings of the Ginseng society Conference
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• 2005.05a
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• pp.19-19
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• 2005

• Journal of Animal Environmental Science
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• v.13 no.1
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• pp.45-52
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• 2007

To evaluate the feed value of starfish, antimicrobial effects of its extract, nutrients contents, concentration of amino acids and its bioavailability were tested. Steaming and ether processes were applied to obtain the extract from starfish for antimicrobial effects examination. The starfish was dried at $60^{\circ}C$ for 3 days before grinding for processing and fermentation. Ground starfish(GS), extruded starfish(ES), fermented starfish(EFS) were added with enzyme and without enzyme(Non enzyme fermented starfish : NEFS). Then the nutrient composition and bioavailability of those were analyzed. The extract from starfish showed no inhibition of the growth of lactobacillus and pathogenic bacteria. Protein content showed significantly higher 62.86% and 52.82%, respectively in EFS and NEFS than GS and EGS(p<0.05). The Ca content of GS, EGS, EFS and NEFS was 17.26%, 18.26%, 5.37% and 8.55%, respectively. It was low in EFS and NEFS due to measure the Ca content after fermentation. Total amino acid was 17.17 mg/g, 20.28 mg/g, 36.30 mg/g and 29.96 mg/g in GS, EGS, EFS and NEFS, respectively. The ratio of total amino acid to protein tended to show the similar tendency as total amino acid. Both total amino acid and its ratio to protein were increased by the fermentation. Bioavailability of the protein and Ca showed more 80% in EFS and NEFS. The nutrients availability of EFS were significantly higher in laying hens than other treatments. The results of these experiments indicate that starfish would be applied as a feed ingredients if it was properly treated.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.3
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• pp.1341-1349
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• 2011

This research is focused on an analysis on the structure of RAW image file and the development of a RAW image file viewer for an embedded device. Recently, several RAW image file formats are being used for saving and displaying the images created by various DSLR cameras, and the necessity of handing RAW images in mobile multimedia devices is increasing. For the development of RAW image decoding/encoding library applicable to WinCE-based embedded devices viewer, an analysis of RAW image file formats, such as CRW, CR2, PEF, NEF, MRW, have been performed because their formats are not released in public. By using the library, the analysis software which can extract RAW image data, 2~3 JPEG image files and other informations such as the specification of a camera and various photographic parameters from RAW image files, were developped and a RAW image file viewer which can run in WinCE-based embedded devices. The experimental result has shown that the viewer could encode and decode RAW image files successfully and it took approximately 10secs to load them to the screen in S3C6410 based embedded platform. The outcomes of this research cloud be a good information and solution to multimedia application developers.

• Proceedings of the Ginseng society Conference
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• 2003.05a
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• pp.50-51
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• 2003

• Venture DIGEST
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• s.131
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• pp.38-39
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• 2009

페루 요즘 이 낯설고 먼 나라가 심상치 않다. 300년간 스페인의 식민 지배 수탈과 19세기 말 칠레에게 국토의 요충지를 빼앗긴 슬픈 역사를 갖고 있는 페루는 수많은 군사 쿠데타와 독재, 정치적 분쟁, 사회적 경제적 혼돈을 딛고 최근 역사상 최고의 호황기를 맞고 있다. 일인당 국민소득 $3,400, 빈곤비율 40%, 전 인구의 14%가 절대 빈곤 상태이지만, 행복지수 세계 33위(한국 102위, 2008년 영국 NEF 발표)를 기록한 페루. 게다가 세계 35위(2008넌 스위스 IMD)의 국가경쟁력으로 31위의 우리나라를 바짝 쫓아오고 있다. 이처럼 눈부시게 성장하고 있는 페루를 주목해보자.

• Proceedings of the Ginseng society Conference
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• 2004.05a
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• pp.45-47
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• 2004

• Journal of Ginseng Research
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• v.43 no.2
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• pp.312-318
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• 2019

Background: To date, no study has described disease progression in Asian patients infected with HIV-1 subtype D. Methods: To determine whether the disease progression differs in patients infected with subtypes D and B prior to starting combination antiretroviral therapy, the annual decline (AD) in $CD^{4+}$ T cell counts over $96{\pm}59months$ was retrospectively analyzed in 163 patients and compared in subtypes D and B based on the nef gene. Results: $CD^{4+}$ T cell AD was significantly higher in the six subtype D-infected patients than in the 157 subtype B-infected patients irrespective of Korean Red Ginseng (KRG) treatment (p < 0.001). Of these, two subtype D-infected patients and 116 subtype B-infected patients had taken KRG. AD was significantly lower in patient in the KRG-treated group than in those in the $KRG-na{\ddot{i}}ve$ group irrespective of subtype (p < 0.05). To control for the effect of KRG, patients not treated with KRG were analyzed, with AD found to be significantly greater in subtype D-infected patients than in subtype B-infected patients (p < 0.01). KRG treatment had a greater effect on AD in subtype D-infected patients than in subtype B-infected patients (4.5-fold vs. 1.6-fold). Mortality rates were significantly higher in both the 45 $KRG-na{\ddot{i}}ve$ (p < 0.001) and all 163 (p < 0.01) patients infected with subtype D than subtype B. Conclusion: Subtype D infection is associated with a >2-fold higher risk of death and a 2.9-fold greater rate of progression than subtype B, regardless of KRG treatment.