• 대한화장품학회지
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• 제41권2호
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• pp.151-157
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• 2015

본 연구에서는 기능성 화장품 소재 개발을 위하여 비파엽 추출물을 제조하고 효소 및 세포 활성평가를 진행하였다. 활성평가는 tyrosinase 측정법을 이용한 미백 효능, NO 측정법을 이용한 항염 효능에 대한 활성등을 분석하였다. 미백 효능 관련 tyrosinase 활성 저해는 10% 농도의 비파엽 초임계 추출물에서 미약한 활성을 나타내었다. 항염 효능 관련 NO 활성 저해 분석 결과, 초임계 추출물, 에탄올 환류 추출물, 열수 추출물의 순서로 NO 억제 활성을 나타내었다. MTT assay를 실시한 결과, 70% 에탄올 환류 추출물 5% 처리를 제외하고는 모든 농도에서 세포독성이 관찰되지 않았다. 미백 효능 관련 멜라닌 생성 저해는 5% 처리 농도에서 30%, 70% 에탄올 환류 추출물이 강하게 멜라닌 생성을 억제하는 결과를 보였다. 이상의 연구결과들로부터 비파엽 추출물 제조를 통한 우수한 항염 효능 및 미백 효능 관련 활성을 나타내는 추출조건을 도출하였으며, 향후 기능성 화장품 소재 개발을 위한 기초 연구용 자료로서의 역할을 제시할 수 있을 것이다.

• Korean Chemical Engineering Research
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• 제52권6호
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• pp.701-705
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• 2014

본 연구에서는 꾸지뽕나무의 뿌리, 줄기, 열매 및 잎으로부터 유효성분을 열수 추출하고 다양한 화장품 소재 시험을 실시하여 화장품 소재로서의 개발 가능성을 검토하였다. 세포독성시험에서 꾸지뽕나무의 뿌리, 줄기 그리고 열매 추출물은 독성을 나타내지 않았지만 잎 추출물은 $200{\mu}g/ml$ 이상의 농도에서 다소의 독성을 나타내었다. 꾸지뽕나무 뿌리추출물은 $500{\mu}g/ml$의 농도에서 81%의 tyrosinase 억제효과(미백효과)와 58%의 elastase 억제효과(주름개선효과)를 나타내었고, UVB 자외선 흡수능도 다소 보여주었다. 21일간의 온도 안정성 시험 결과, 꾸지뽕 뿌리 추출물을 포함한 액제와 로션제형은 $25^{\circ}C$에서는 안정하였으나 $47^{\circ}C$에서 점도와 입자크기가 불안정하였다. 본 연구 결과 꾸지뽕 뿌리추출물은 높은 폴리페놀 및 플라보노이드의 함량, 우수한 미백 및 주름개선 효과를 나타내어 천연 기능성 화장품 소재로서 높은 가능성을 보여주었다.

• 약학회지
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• 제45권5호
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• pp.522-528
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• 2001

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidase activity of tyrosinase. For the inhibitory effect against DOPA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and water, respective The methylene chloride fractions of Angelica tenuissima, Nardostachys chinensis, Bombyx mop and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

• 대한화학회지
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• 제67권2호
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• pp.137-144
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• 2023

In this study were evaluated the whitening and anti-oxidative activities from the extracts of Sorbus alnifolia branches, and identified the chemical structures of the active ingredients. In the whitening tests using α-MSH stimulated B16F10 melanoma cells, the 70% ethanol extract and n-butanol (n-BuOH) fractions concentration-dependently inhibited cellular melanogenesis and intracellular tyrosinase activities without causing cell toxicity. The total polyphenol content of n-BuOH and ethyl acetate (EtOAc) fractions were measured to be respectively 241.1 ± 1.1 and 222.9 ± 2.4 (mg/g GAE), and the total flavonoid content of EtOAc fraction was 75.3 ± 2.0 (mg/g QE). Upon anti-oxidant studies with DPPH and ABTS⁺ radicals, potent radical scavenging activities were observed in the EtOAc and n-BuOH fractions. Moreover, in the study of cell protection efficacy using HaCaT keratinocytes damaged by H₂O₂, the EtOAc and n-BuOH fractions showed a very positive results on prevention of oxidative stress. Phytochemical studies for this extract resulted in the isolation of four compounds; 2-oxopomolic acid (1), euscaphic acid (2), epi-catechin (3), prunasin (4). These results suggested that the extract of S. alnifolia branches containing compounds 1-4 as natural ingredients could be used as whitening and anti-oxidant ingredients in cosmetic formulations.

• 한국미생물·생명공학회지
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• 제52권1호
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• pp.65-75
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• 2024

The growing demand for functional cosmetics, driven by modern individuals' quest for healthy and aesthetically pleasing skin, is being challenged by reports of side effects and toxicity associated with these products. This underscores the importance of exploring natural plant-based materials for functional cosmetics. This research focuses on the assessment of antioxidant and whitening properties of mixed extracts from Ipomoea nil, Arctostaphylos uva-ursi, and Angelica gigas Nakai, all of which have proven pharmacological benefits. The study evaluated the extracts' total polyphenol and flavonoid contents, ABTS and DPPH radical scavenging activities, SOD-like activity, and xanthine oxidase inhibition to determine their antioxidant capabilities. Moreover, the whitening potential was investigated through tyrosinase activity and melanin production assays, alongside a cytotoxicity evaluation via a cell viability test. The findings revealed that the extracts, IAA-1 to IAA-4, demonstrated both antioxidative and whitening capabilities without exhibiting cytotoxic effects. Notably, the extract IAA-4, processed through ultrasonic and ultrahigh pressure extraction, exhibited superior effectiveness. These results indicate that the cavitation formed during ultrasonic irradiation effectively destroys the plant cell wall by creating high pressure, and as a result, it is judged that useful components are easily extracted. As a result of the study, it was confirmed that the IAA-4 extract could be applied as a material for functional cosmetics.

• 한국응용과학기술학회지
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• 제28권2호
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• pp.161-169
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• 2011

To develope new natural substances for whitening agent, Baeckji(Angelica dahuria;BK), Seasin(Asarum sieboldii;SS). Baecjgangjam(Bombycis corpus;BGJ) and Mynkanbang(MKB) were selected and extracted by hot water and 70% EtOH, respectively. Hydrothermal and ethanolic extracts of BK, SS, BGJ and MKB were evaluated for anti-oxidative effect, tyrosinase activity, melanogenesis of B16 melanoma cells and changes in level of tyrosinase expression by using Western blotting. All hydrothermal and ethanolic extracts showed scavenging activities of free radicals against 1,1-diphenyl-2-picrylhydrazyl(DPPH) but no inhibitory effect on tyrosinase activity. Ethanolic extracts of BK, SS, and BGJ and especially highly, those of MKB inhibited production of melanin in B16 melanoma cells and were able to reduce the level of tyrosinase expression in B16 melanoma cells. These results suggest that ethanolic extracts of BK, SS, BGJ and MKB can be an effective whitening agent from natural plant.

• 한국미생물·생명공학회지
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• 제52권1호
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• pp.55-64
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• 2024

The desire of modern people to maintain a healthy and beautiful appearance is increasing day by day along with the increasing interest in skin health and the demand for functional cosmetics. Accordingly, research on functional cosmetic materials with few side effects and excellent efficacy is being actively conducted. Therefore, this study tried to verify the antioxidant and whitening effects of the mixed extracts of Bixa orellana, Ammi majus, and Glycyrrhiza glabra, whose efficacy has been individually verified. Extracts (BAG-1~4) with different extraction methods such as steaming, fermentation, and ultrasonication were prepared for 3 types of natural plants, and antioxidant and whitening effects of these extracts were confirmed. For this purpose, antioxidant, tyrosinase activity, melanin production and stability experiments were conducted. Extracts (BAG-1~4) had no cytotoxicity, and antioxidant and whitening effects were confirmed. BAG-4 extracted by steaming and fermentation showed the best efficacy. It seems that enzymes such as lipase, protease, and amylase increase phenol components by various yeasts involved in the fermentation process, thereby improving antioxidant and melanin production inhibitory effects. It was confirmed that the three types of natural plant extracts could be used as safe and functional cosmetic materials.

• 대한음성학회지:말소리
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• 제67호
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• pp.135-147
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• 2008

In this paper, we propose a method for source localization in reverberant environments based on an adaptive eigenvalue decomposition (AED) algorithm which directly estimates channel impulse responses from a speaker to microphones. Unfortunately, the AED algorithm may suffer from whitening effects on channels estimated from temporally correlated natural sounds. The proposed method which applies sparse priors to the estimated channels can avoid the temporal whitening and improve the performance of source localization in reverberant environments. Experimental results show the effectiveness of the proposed method.

• The Journal of the Acoustical Society of Korea
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• 제25권4E호
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• pp.148-153
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• 2006

A new normalized MBD algorithm is presented for nonstationary convolutive mixtures and its properties/modifications are discussed in details. The proposed algorithm normalizes the signal spectrum in the frequency domain to provide faster stable convergence and improved separation without whitening effect. Modifications such as nonholonomic constraints and off-diagonal learning to the proposed algorithm are also discussed. Simulation results using a real-world recording confirm superior performanceof the proposed algorithm and its usefulness in real world applications.

• The Journal of the Acoustical Society of Korea
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• 제24권2E호
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• pp.54-60
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• 2005

A new multichannel blind deconvolution algorithm is proposed for speech mixtures. It employs unidirectional filters and normalization of gradient terms in the frequency domain. The proposed algorithm is shown to be approximately nonholonomic. Thus it provides improved convergence and separation performances without whitening effect for nonstationary sources such as speech and audio signals. Simulations using real world recordings confirm superior performances over existing algorithms and its usefulness for real applications.