• Molecules and Cells
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• 제40권4호
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• pp.291-298
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• 2017

MicroRNAs (miRNAs) are short non-coding RNAs that regulate genes posttranscriptionally. Past studies have reported that miR-210 is up-regulated in many cancers including cervical cancer, and plays a pleiotropic role in carcinogenesis. However, its role in regulating response towards anti-cancer agents has not been fully elucidated. We have previously reported that the natural compound 1'S-1'-acetoxychavicol acetate (ACA) is able to induce cytotoxicity in various cancer cells including cervical cancer cells. Hence, this study aims to investigate the mechanistic role of miR-210 in regulating response towards ACA in cervical cancer cells. In the present study, we found that ACA down-regulated miR-210 expression in cervical cancer cells, and suppression of miR-210 expression enhanced sensitivity towards ACA by inhibiting cell proliferation and promoting apoptosis. Western blot analysis showed increased expression of mothers against decapentaplegic homolog 4 (SMAD4), which was predicted as a target of miR-210 by target prediction programs, following treatment with ACA. Luciferase reporter assay confirmed that miR-210 binds to sequences in 3'UTR of SMAD4. Furthermore, decreased in SMAD4 protein expression was observed when miR-210 was overexpressed. Conversely, SMAD4 protein expression increased when miR-210 expression was suppressed. Lastly, we demonstrated that overexpression of SMAD4 augmented the anti-proliferative and apoptosis-inducing effects of ACA. Taken together, our results demonstrated that down-regulation of miR-210 conferred sensitivity towards ACA in cervical cancer cells by targeting SMAD4. These findings suggest that combination of miRNAs and natural compounds could provide new strategies in treating cervical cancer.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권4호
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• pp.235-246
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• 2021

This study investigated the impact of baseflow on fish community in the Ungcheon stream (16.5 km long) located downstream of the Boryeong Dam, Korea. Based on field monitoring, there were five dominant fish species in the Ungcheon Stream accounting for 75% of the total fish community: Zacco platypus, Zacco koreanus, Tridentiger brevispinis, Rhinogobius brunneus, and Pungtungia herzi. These five fish species were selected as target species. HydroGeoSphere (HGS) and River2D models were used for hydrologic and hydraulic simulations, respectively. A habitat suitability index model was used to simulate fish habitat. To assess the impact of baseflow, each representative discharge was examined with or without baseflow. The HGS model was used to calculate baseflow within the study reach. This baseflow was observed to increase gradually with longitudinal distance. Validation of the hydraulic model dem onstrated that computed water surface elevated when baseflow was included, which was in good agreement with measured data, as opposed to the result when baseflow was excluded. Composite suitability index distributions and weighted usable area in the study reach were presented for target species. Simulations indicated that the baseflow significantly increased habitat suitability for the entire fish community. These results demonstrate that there should be a substantial focus on the baseflow for physical habitat simulation.

• 한국산림과학회지
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• 제86권1호
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• pp.17-24
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• 1997

지구(地球)의 잠재자연식생분포(潛在自然植生分布)를 추정(推定)하기 위한 첫단계로서 자연식생분포(自然植生分布)와 밀접(密接)한 관계가 있는 기온(氣溫)과 강수량(降水量)에 대한 최적구면보간방법(最適球面補間方法)을 개발하기 위해, Light Climatic Dataset로 명명(命名)한 전세계에 분포(分布)된 1,060점의 측후소(測候所)의 실측자료(實測資料)를 보간용(補間用)과 검정용(檢定用)으로 무작위(無作爲) 2등분(等分)하고, 검정용(檢定用) 측후소(測候所)에 있어서 관측치(觀測値)와 보간용(補間用)에 의한 추정치(推定値)와의 오차(誤差)를 보간방법(補間方法)과 파라메타가 틀린 조합(組合)에 대해 상호비교(相互比較)하였다. 보간용(補間用)에 의한 추정치(推定値)는, 측후소(測候所)의 선택법(選擇法)으로 전점(全点), 정점(定点), 안반경(安半徑)의 3가지 방법, 거리에 대한 하중(荷重)으로 n승 반비례(反比例), 부(負)의 지수함수(指數函數)의 2가지 방법, 방위(方位)에 관한 하중(荷重)으로 타원하중법(楕圓荷重法), 각각에대해 상호조합(相互組合)하여 산출(算出)되었다. 그 결과, 측후소(測候所)의 선택법(選擇法)은 정반경법(定半徑法), 거리(距離)에 관한 하중식(荷重式)은 부(負)의 지수함수(指數函數), 방위(方位)에 관한 하중법(荷重法)은 타원하중법(楕圓荷重法)을 조합(組合)하는 것이 기온(氣溫)과 강수량(降水量)의 보간(補間)에 적합(適合)하였으며, 그에 관한 파라메타 값은 기온(氣溫)에 있어서 정반경(定半徑) $30^{\circ}$, -0.78의 지수함수(指數函數), 타원하중(楕圓荷重) 0.4로, 강수량(降水量)에 있어서는 각각 $35^{\circ}$, -0.53, 1.2로 나타났다.

• Archives of Pharmacal Research
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• 제20권5호
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• pp.425-431
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• 1997

To examine components of Ganoderma lucidum for anti-human immunodeficiency virus (HIV) activity, the aqueous extracts of its basidiocarps were separated into high-molecular-weight (HMF) and low-molecular-weight (LMF) fractions. These fractions were used in XTT [2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide] antiviral assay which can quantitatively measure cytopathic effects of HIV-1 on CEM, human T lymphoblastoid cell line. The CEM cell line added with serial diluted HMF or LMF was cultured in the absence or presence of HIV-1. The results showed that the LMF of the aqueous extract strongly inhibited cytopathic effect of the target cell induced by HIV-1. When two-fold serially diluted LMF ranging from $40.97{\mu}g/ml$4 to 125.00 .mu.g/ml was added to the virus-free culture system, no toxicity on the target cells was detected in all the concentrations tested. However, when it was added to the HIV-infected culture system, the viabilities of the target cell reached a plateau recovering its viabilities to 71.7% and 82.5% in experiment-1 and -2 at 15.60 .mu.g/ml, respectively. The cell viabilities were then gradually decreased but maintained at more than 50% above 31.20 .mu.g/ml concentration. On the contrary, HMF did not prevent any HIV-induced cytopathic effect at any concentrations tested on this cell line. From these results, negligible toxicities were observed by both HMF and LMF of G. luciolum, and recovery of cell viability in HIV infected target cell was induced only by LMF of the carpophores.

• 한국태양에너지학회 논문집
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• 제36권5호
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• pp.31-50
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• 2016

To develope a greenhouse fog cooling system to control the temperature and relative humidity simultaneously to the target value, a theoretical analysis and experiments were done. The control process includes the measuring of environmental variables, setting and coding of the water and heat balance equations to maintain the target temperature and relative humidity in greenhouse, calculating of the open level of the greenhouse roof window that governs the natural ventilation and spray water quantity, and operating of the motor to open/close the roof window and pump to spray for water. The study results were shown to be very good because the average air temperature in the greenhouse was kept to be about $28.2^{\circ}C$ with the standard deviation of about $0.37^{\circ}C$ compared to the target temperature of $28^{\circ}C$ and the average relative humidity was about 75.2% compared to the target relative humidity was 75% during the experiments. The average outside relative humidity was about 41.0% and the average outside temperature was $27.2^{\circ}C$ with the standard deviation of about $0.54^{\circ}C$. The average solar intensity in the greenhouse was 712.9 W. The wind velocity of outside greenhouse was 0.558 m/s with the standard deviation of 0.46 m/s.

• 대한가정학회지
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• 제31권3호
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• pp.175-186
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• 1993

The present study was designed to find out the construction conditions of tight skirt for sale in relation to its brand, target age and length. The 15 tight skirts each from 3 brand groups(designer character, national and minor brand) of 2 target age groups(youth and middle age) and the 15 tight skirts each from 3 skirt length groups(mini, natural line and maxi) of only the minor brand-a total of the 120 tight skirts on the market-were measured. The data were analyzed with use of SAS package. The statistics were based on frequency, percentage, average, standard deviation, one-way ANOVA, two-way ANOVA, Pearson's correlation and linear regression analysis. The main results were as follows. 1. There were significant differences in the construction conditions of tight skirt according to its brand and target age. There was a significant difference particurarly in the difference between hip girth and hem girth according to brand target age. The difference between hip girth and hem girth of designer's character brand skirt was larger than that of national brand & minor brand skirt, and that of skirt targeting the youth was larger than that of skirt targeting the middle aged. 2. There were significant difference particurarly in the differences in the construction conditions of tight skirt according to its length. There was a significant difference particurarly in the difference between hip girth and he girth according to skirt length, and that of maxi skirt was larger than that of mini & natural line skirt. There was a good correlation between skirt length and slit length and between skirt length and skirt length above the slit. The longer the skirt length was, the longer the slit length and the skirt length above the slit were.

• 한국환경복원기술학회지
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• 제13권5호
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• pp.38-50
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• 2010

Expressways are often upgraded by widening the lanes and/or by reshaping the roads to be more linear for faster travel time. However, during the process of improving the route of the expressways, the areas where the old expressways used to be are often unused and abandoned. When these neglected sites are left alone, they often become dump sites causing pollution and impacting the surrounding environment. Therefore, it is important to restore the abandoned expressway sites to its full natural beauty. In this study, the abandoned expressway at the Soksa interchange in Pyungchang county, located in Kangwon province was studied for establishing the model of ecological restoration project. Considering the characteristics of the site, the target flora species was chosen to be Quercus species and the target fauna species as amphibians. After the target species were carefully chosen, each species' habitat requirements were studied in order to figure out the appropriate methods toward habitat restoration specifically for these species. In addition, to determine the most efficient method toward restoration of abandoned expressways, the study utilized the planting hole techniques, the crack techniques, and the colonization techniques. In terms of the spatial organization, public education program is incorporated at the main entrance area and the programs for experimenting, and developing vegetation and habitat restoration techniques are placed in the vicinity. In the master plan-to provide natural ecosystem at the site-ASCON (asphalt concrete) was removed first, then plans for restoration including species' habitat restoration were established. Furthermore, the project included plans for improving water quality polluted through non-point source considering the surrounding nearby road and farm lands. Finally, the study established a planning process that will experimentally apply to the abandoned expressway restoration method. In the future, there will be a continuous monitoring of the methods applied to verify if the restoration methods are effective. Also, new restoration techniques should be available according to a variety of abandoned expressways' characteristics.

• Bulletin of the Korean Chemical Society
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• 제34권11호
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• pp.3345-3349
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• 2013

Hsp90 shows great promise as a therapeutic target due to its potential to disable multiple signaling pathways simultaneously. In this study, we discovered that a natural product, butein moderately inhibited the growth of drug-resistant cancer cells (A2780cis and H1975), and brought about the degradation of oncogenic Hsp90 client proteins. The study demonstrated that butein would be a therapeutic lead to circumvent drug-resistance in cancer chemotherapy. The structure-based screening, synthesis, and biological evaluation of butein are described herein.

• 대한약침학회지
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• 제25권2호
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• pp.145-147
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• 2022

• 공업화학
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• 제18권1호
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• pp.1-9
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• 2007

천연가스는 가솔린에 비하여 에너지 밀도가 매우 낮아 천연가스 차량의 경우 약 24.8 MPa로 압축된 압축천연가스(CNG)를 이용하기 때문에 고압을 얻기 위하여 다단계 압축이 요구될 뿐 아니라 안정성에 문제가 많다. 이런 이유로 비교적 낮은 압력에서 저장할 수 있는 흡착천연가스에 관심을 갖게 되었다. 천연가스의 저장에 용이한 매질을 개발하여 3.5 MPa에서 CNG와 에너지 밀도가 유사하고, 같은 용적의 가솔린에 비하여 1/4 수준의 운전거리를 확보하는 것이 현재의 목표이다. 본 총설에서는 흡착천연가스(ANG) 저장을 위한 탄소매질의 개발현황, 매질의 특성 및 실용화를 위하여 진행되고 있는 내용을 간략하게 소개하고 몇 가지 필요한 제언을 한다.