• Proceedings of the Korea Contents Association Conference
• /
• 2006.11a
• /
• pp.682-686
• /
• 2006

There have been increasing interests in 3D mobile content service with emergence of a terminal equipping with a mobile 3D engine and growth of mobile content market. Mobile 3D Avatar is the most effective product displaying the character of a personalized mobile device user. However, previous studies on the method of expressing 3D Avatar have been mainly focused on natural and realistic expressions according to the change in facial expressions and lip shape of a character in PC based virtual environments. In this paper, we propose a method of synchronizing the lip shape with voice by applying a SMS message received in mobile environments to 3D mobile Avatar. The proposed method enables to realize a natural and effective SMS message reading service of mobile Avatar by disassembling a received message sentence into units of a syllable and then synchronizing the lip shape of 3D Avatar with the corresponding voice.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.2
• /
• pp.258-263
• /
• 2000

2-Hydroxy-6-oxo-6phenylhexa-2,4-dienoate (HOPDA) hydrolase catalyzes the hydrolytic cleavage of HOPDA to bemzpate and 2-hydroxypenta-2, 4-dienoate (HPD) during microbial catabolism of biphenyl and polychlorinated biphenyls. A HOPDA hydrolase gene (pcbD) was isolated from the genomic library of Pseudomonas sp. P20 and designated as pCNUO1201; a 7.5-kb XbaI DNA fragment from Pseudomonas sp. P20 was inserted into the pBluescript SK(+) XbaI site. E. coli HB101 harboring pCNU1201 exhibited HOPDA hydrolase activity. The open reading frame (ORF) corresponding to the pcbD gene consisted of 855 base pairs with an ATG initiation codon and a TGA termination codon. The ORF was preceded by a rebosome-binding sequence of 5'-TGGAGC-3' and its G+C content was 55 mol%. The pcbD gene of Pseudomonas sp. P20 was located immedeately downstream of the pcbC gene encoding 2,3- dihydroxybiphenyl 1,2-dioxygenase, and approximately 4-kb upstream of the pcbE gene encoding HPD hydratase. The pcbK gene was able to encode a polypeptide with a molecular weight of 31,732 containing 284 amino acid residues. The deduced amino acid sequence of the HOPDA hydrolase of Pseudomonas sp. P20 exhibited high identity (62%) with those of the HOPDA hydrolases of P. putida KF715, P. pseudoalcaligenes KF707, and Burkholderia cepacia LB400, and also significant homology with those of other hydrolytic enzymes including esterase, transferase, and peptidase.

• Journal of the Korean School Mathematics Society
• /
• v.8 no.4
• /
• pp.481-494
• /
• 2005

In this study, the goal is to spread profound knowledge and theory through providing with accumulated methods in mathematics education to the students who are relatively neglected in educational benefits. The process is divided into 3 categories: mathematics for obtaining common sense and intelligence, practical math for application, and math as a liberal art to elevate their characters. Furthermore, it includes the reasons for studying math, improving problem-solving skills, machinery application learning, introduction to code(cipher) theory and game theory, utilizing GSP to geometry learning, and mathematical relations to sports and art. Based on these materials, the next step(goal) is to train graduate students to conduct researches in teaching according to the teaching plan, as well as developing interesting and effective teaching plan for the remote high school learners.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.12
• /
• pp.1653-1663
• /
• 2010

The first gene (${\alpha}$-gal1) encoding an extracellular ${\alpha}$-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The ${\alpha}$-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a 24 amino acid secretory signal sequence. The translated protein had highest identity with other fungal ${\alpha}$-galactosidases belonging to glycosyl hydrolase family 27. The ${\alpha}$-gal1 gene was overexpressed as a secretory protein with an N-terminal histidine tag in the methylotrophic yeast Pichia pastoris. Recombinant ${\alpha}$-Gal1 was secreted into the culture medium as a monomeric glycoprotein with a maximal yield of 10.75 mg/l and purified to homogeneity using Hisbinding nickel-agarose affinity chromatography. The purified enzyme was maximally active at $70^{\circ}C$, pH 4.5, and lost no activity over 10 days at $50^{\circ}C$. ${\alpha}$-Gal1 followed Michaelis-Menten kinetics ($V_{max}\;of\;240.3{\mu}M/min/mg,\;K_m\;of\;0.294 mM$) and was inhibited competitively by galactose ($K_m{^{obs}}$ of 0.57 mM, $K_i$ of 2.77 mM). The recombinant T. emersonii ${\alpha}$-galactosidase displayed broad substrate preference, being active on both oligo- and polymeric substrates, yet had strict specificity for the ${\alpha}$-galactosidic linkage. Owing to its substrate preference and noteworthy stability, ${\alpha}$-Gal1 is of particular interest for possible biotechnological applications involving the processing of plant materials.

• Journal of Elementary Mathematics Education in Korea
• /
• v.16 no.1
• /
• pp.21-38
• /
• 2012

In this study, elementary mathematics textbooks from grade 1 to grade 6 of Korea and Yanbian Korean Autonomous Prefecture are compared. This study is limited to number area. In this study, textbooks of Korea are developed according to the 2007 curriculum and published between 2009 and 2011 and textbooks of Yanbian are published between 2009 and 2010. Seven implications for developing Korean textbooks can be drew from Yanbian textbooks as conclusions. First, it is necessary to consider using counters to teach place values. Second, it is necessary to consider reading inequalities in style of one to one correspondence between signs and words. Third, it is necessary to consider mentioning explicitly that it is possible to express the concrete whole which has a continuous quantity as natural number 1. Fourth, it is necessary to consider introducing term of fraction line that separates the numerator and denominator. Fifth, it is necessary to consider mentioning explicitly the properties of the fraction. Sixth, it is necessary to consider broadening examples to use decimals. Seventh, it is necessary to consider stating clearly that it is possible to make an additional place of decimals by adding a zero at the right end of the decimals.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.10
• /
• pp.1570-1576
• /
• 2006

An encephalomyocarditis virus (EMCV-CBNU) was isolated from an aborted swine fetus in October 2005. To investigate the genetic origin and virulence of the EMCV-CBNU strain, we determined the complete sequence of the virus and tested its virulence in mice. Genetic characterization revealed that the RNA genome was composed of 7,713 nucleotides with a single open reading frame (2,292 amino acids), coding 12 proteins. The EMCV-CBNU had the shortest poly(C) tract, consisting of 10 C's ($C_{10}$), compared with all the other EMCV strains reported in GenBank. Amino acid and phylogenetic analyses showed that EMCV-CBNU had the highest genetic identity with strain 2887A (99.7%), which was originally isolated from a fetus in a pig breeding farm that had a history of reproductive failure. Because rodents are the natural host of EMCV, we investigated the virulence of EMCV-CBNU in mice. Surprisingly, all mice inoculated with more than $1{\times}10^2\;TCID_{50}/0.1ml$ of EMCV-CBNU showed symptoms of hind limb paralysis and eventually died during 3 and 8 days postinoculation (DPI). Furthermore, when we inoculated the virus into pregnant mice, all dams and their fetuses died in 6 DPI. This is the first report on a full genomic analysis of swine EMCV in Korea, which exhibits high virulence in mice.

• Journal of Korean Philosophical Society
• /
• no.85
• /
• pp.53-76
• /
• 2009

Plato's Laws, Bk. 10 is made up of cryptically concise expressions and complex arguments, so that it is not simple to understand it. In this essay I would like to make use of Stoic perspective as an interpretative instrument, which would enable me, I hope, to reconstruct the main arguments of the Laws, Bk. 10 into an intelligible form. Through this approach we would have an opportunity to meet with some important philosophical ideas of Plato which did not reveal themselves clearly in other, especially early and middle dialogues. These ideas comprise the inseparability of the soul from the body, the intellectualization of nature, the human being as a part of the cosmos and the extensional overlapping of the moral law and the natural law. And at the same time my study would result in making a suggestion for history of reception. That is, in this paper I will find some grounds in the tenth book of the Laws that would show how great the influence of Plato upon the Stoics was. For we have but little knowledge about the possibility of the power of Plato's influence upon the Stoics, even though the scholarship on the Stoics until now shed some light on the features and range of Aristotle's influence upon them.

• Ocean Science Journal
• /
• v.40 no.1
• /
• pp.55-59
• /
• 2005

We cloned the complete cDNA of the ${\beta}-bubulin$ from the soft coral, Scleronephthya gracillimum $(K\ddot{u}kenthal)$ (Alcyonacea, Octocorallia, Anthozoa, Cnidaria), via the random sequencing of a cDNA library and the 5'-rapid amplification of cDNA end (RACE) technique. The full-length cDNA of the S. gracillimum ${\beta}-tubulin$ comprised 1541 bp, not including the poly $A^+$ stretch, also contained a complete open reading frame, which codes for a total of 445 amino acids. The amino acid residues 16402 appeared to be in a state of conservation in a variety of animals. Northern blot analysis clearly demonstrated that the sequence we have obtained is, indeed, the full-length cDNA of the ${\beta}-bubulin$ gene in S. gracillimum.

• The Journal of the Acoustical Society of Korea
• /
• v.18 no.3
• /
• pp.17-26
• /
• 1999

We present the first speech coarticulation database of Korean, English and Konglish/sup 3)/ named "SORIDA"/sup 4)/, which is designed to cover the maximum number of representations of coarticulation in these languages [1]. SORIDA features a compact database which is designed to contain a maximum number of triphones in a minimum number of prompts. SORIDA contains all consonantal triphones and vowel allophones in 682 Korean prompts of word length and in 717 English prompt words, spoken five times by speakers of balanced genders, dialects and ages. Korean prompts are synthesized lexicons which maximize their coarticulation variation disregarding any stress phenomena, while English prompts are natural words that fully reflect their stress effects with respect to the coarticulation variation. The prompts are designed differently because English phonology has stress while Korean does not. An intermediate language, Konglish has also been modeled by two Korean speakers reading 717 English prompt words. Recording was done in a controlled laboratory environment with an AKG Model C-100 microphone and a Fostex D-5 digital-audio-tape (DAT) recorder. The total recording time lasted four hours. SORIDA CD-ROM is available in one disk of 22.05 kHz sampling rate with a 16 bit sample size. SORIDA digital audio-tapes are available in four 124-minute-tapes of 48 kHz sampling rate. SORIDA′s list of phonetically-rich-words is also available in English and Korean.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.6
• /
• pp.823-828
• /
• 2000

The complete nucleotide sequence of hepatitis B virus DNA isolated from Korean patient serum was determined and characterized, and its phylogenetic relation was then investigated. The viral genome was 3,215 base pairs long and included four well known open reading frames (i.e. surface antigens, core antigens, X protein and DNA polymerase). The sequence of the surface antigen showed that the HBV genome under investigation, designated HBV 315, was characteristic of subtype adr. A phylogenetic analysis using the total genome sequence revealed that HBV315 was grouped into genomic group C together with isolates from Japan, China, Thailand, Polynesia, and New Caledonia. The mean percent similarity between HBV315 and other HBV isolates in genomic group C was 97.25%, and that with other genomic groups ranged from 86.16% to 91.25%. The predicted amino acid sequences of HBV315 were compared with two closely related subtype adr isolates, M38636 and D12980. The results showed that the X gene product was identical in the three strains, while there were significant amino acid sequence differences between HBV315 and M38636 in the Pre-S1 and Pre-S2 regions.