• 대한한방내과학회지
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• 제33권2호
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• pp.197-208
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• 2012

Objectives : Gleditsiae Spina has the effects of expelling toxins, draining pus, invigorating blood and resolving abscesses. Some clinicians apply the herb for patients suffering from cancer. However, its anti-cancer activities are not well understood. In the present study, anti-tumor agents from Gleditsiae Spina were purified. Methods : The viability of the PC-3 cell line was determined using MTT assay, and the induction of apoptosis by Gleditsiae Spina extract in PC-3 cells was measured by Annexin-V/propidium iodide double staining assay detected by flow cytometry. TLC and HPLC analysis were used to separate and identify the anti-cancer agents. Results : Treatment of the extract resulted in significant decreased cell viability of PC-3 cells in a dose- and time-dependent manner. Dose-dependent apoptotic cell death was also measured by flow cytometry analysis. The anti-cancer agents were successfully separated and identified by using TLC and HPLC analysis and the most potential agent among them was separated from EtOAC fraction. Conclusions : These results might be applied in developing new drugs from natural resources like Korean traditional medicine, and also support the clinical usefulness of herbal medicine.

• 환경영향평가
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• 제23권3호
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• pp.197-207
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• 2014

In this study, the functional priority of wetlands was determined through analysis of previous research. To determine relative importance, three processes were performed. First, quantitative values from the case studies were normalized. Second, non-quantitative values were prioritized based on standard criteria. Third, equal weight was applied as long as there was no special consideration regarding a particular value's disproportionate priority in the research. Finally, results were grouped into large, medium, and small classes.In this study, the functions of the medium class were found to be the most significant, in the following order of priority: water supply and ground water recharge; culture and recreation; biodiversity; product; water quality control; flood control; erosion control; moderation of climate change; and provision of biochemical matter. To verify these results, we compared our findings with those of an assessment that used the Rapid Assessment Method (RAM) on the same type of study area. Whereas this comparison indicated some correlations by the culture, water storage, and genetic sustainability functions, it suggested a lack of such relationship by the water purification and flood prevention functions.

• 한국응용과학기술학회지
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• 제32권4호
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• pp.685-693
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• 2015

본 연구에서 개발 예정인 해조류 펄프 분리 가공은 해조류자체의 배당체 및 다당류의 복합체로서 개발상 많은 어려움이 있다. 그러나 정제 공정을 개발하고 응용기술을 도입하여 아직 미개척분야인 펄프 응용기술을 확립할 수 있도록 한다. 따라서 본 제품이 개발 대량 생산되어지면 수출향상에 기여함은 물론 생분해성 기능성소재 개발의 실용화로 어민 소득증대 향상에 기여할 수 있다. 따라서 연구의 최종 목표는 1,2차년도의 기술적 데이터를 종합하여 해조류 펄프를 적용하여 생분해성이 향상되고, 고분자의 특수성질을 갖는 새로운 기능성 생분해성 플라스틱, 포장재료 등의 원재료 제품 및 시제품개발을 목표로 한다.

• Molecules and Cells
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• 제28권1호
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• pp.19-24
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• 2009

The goal of this study was to create a novel baculovirus expression system that does not require recombinant virus purification steps. Transfection of insect cells with transfer vectors containing barnase under control of the Cotesia plutellae bracovirus (CpBV) promoters ORF3004 or ORF3005 reduced cell growth. Co-transfection with bApGOZA DNA yielded no recombinant viruses and nonrecombinant backgrounds. To further investigate the detrimental effects of barnase on insect cells, two recombinant bacmids harboring the barnase gene under control of the CpBV promoters, namely bAcFast-3004ProBarnase and bAcFast-3005ProBarnase, were constructed. While no viral replication was observed when only the recombinant bacmids were transfected, recombinant viruses were generated when the bacmids were co-transfected with the transfer vector, pAcUWPolh, through substitution of the barnase gene with the native polyhedrin gene by homologous recombination. Moreover, no non-recombinant backgrounds were detected from unpurified recombinant stocks using PCR analysis. These results indicate that CpBV promoters can be used to improve baculovirus expression vectors by means of lethal gene expression under the control of these promoters.

• Applied Biological Chemistry
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• 제43권2호
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• pp.81-85
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• 2000

미생물로부터 고분자 물질을 생산하여 기능성 식품보조제로 사용하기 위한 일환으로 고점성의 biopolymer를 생산하는 균주를 분리하고 이 분리균에 대한 분류학적 성질 및 생성 biopolymer의 이화학적 성질을 조사하였다. 분리 균주는 Bacillus coagulans로 동정되었으며 Bacillus cuagulans CE-74라 명명하었다. Acetone과 ethanol 처리를 하여 crude biopolymer를 얻었으며, crude biopolymer를 DEAE-cellulose ion exchange cromatography, Sephadex G-100 및 Sepharose CL-2B를 사용하여 fraction I과 fraction II 두 분획을 분리 정제하였다. 분리된 분획의 구성 성분을 분석한 결과 당 성분은 검출되지 않았고, 아미노산 분석을 실시한 결과 fraction I은 lysine만으로 구성되어 있었으며, fraction II는 glutamic acid만이 검출되었다. 분자량은 fraction I은 약 42kD, fraction II는 약 $1.6{\times}10^3\;KD$로 추정되었다.

• 한국습지학회지
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• 제12권3호
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• pp.39-47
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• 2010

최근 우리나라 농경지 면적은 해마다 감소하고 있는 추세이나 이러한 농업지역으로부터 발생되는 오염부하량은 오히려 증가하고 있는 실정이다. 농업지역으로부터 발생되는 오염물질 중 대부분은 비점오염원에 의해 배출되며 이는 주로 저농도이면서 넓은 지역에서 대량으로 배출되는 특성을 보인다. 본 연구의 목적은 농업지역 내 비점오염원을 관리하기 위한 방안으로 인공습지를 적용하여 수질정화효율을 분석함으로써 인공습지 적용가능성과 오염물질 제거기작, 설계인자 등을 제공함에 있다. 모니터링 결과를 토대로 수질분석을 실시하였으며 분석 결과, 유입수의 평균 농도 제거율은 TSS가 26 %, BOD가 28 %, TN이 16 %, TP가 39 %로 나타났다. 또한 국내 하천수질기준에 준하여 인공습지 유입수과 유출수의 수질을 비교해 본 결과 대부분의 항목에서 수질등급이 향상되었다.

• 한국축산식품학회지
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• 제28권1호
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• pp.1-8
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• 2008

최근까지 박테리오신 생산균주의 분리, 분자생물학적 메커니즘, 정제, 구조 및 작용기작, 산업적인 적용 등의 논문 등이 보고되고 있다. 또한 50개국 이상에서 치즈, 통조림식품 등에서 식품보존제로서 승인되어 있다. 박테리오신의 여러 장점으로 인해 현재 추진되고 있는 축산업 분야의 HACCP의 범위인, 사육장에서부터 식품제조에 이르기까지 항생물질, 인공적인 방부제나 식품첨가물이 적게 들어가거나 첨가되지 않은 자연식품을 선호하고 있는 실정에 적합하다. 향후 축산업에서 항생제 대체방안의 하나로서 박테리오신의 사용은 확대되리라 기대된다. 경제적인 가격 경쟁력을 갖기 위해, 대량생산에 대한 연구가 이루어져야 하며, 적절한 제형으로 보다 구체적인 적용실험이 수행되어 산업화를 앞당겨야 한다.

• 한국농공학회논문집
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• 제49권4호
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• pp.51-59
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• 2007

Recently, concrete has been made porous and used for sound absorption, water permeation, vegetation and water purification according to void characteristics. Many studies are carried out on the utilization of sewage sludge, fly ash and waste concrete to reduce the environmental load. This study was performed to evaluate the void, strength, relationship between void and strength, permeability and chemical resistance properties of porous polymer concrete for pavement with different fillers. An unsaturated polyester resin was used as a binder, crushed stone and natural sand were used as an aggregate and bottom ash, fly ash and blast furnace slag were used as fillers. The mix proportions were determined to satisfy the requirement for the permeability coefficient, $1{\times}10^{-2}$ cm/s for general permeable cement concrete pavement in Korea. The void ratios of porous polymer concrete with fillers were in the range of $18{\sim}23%$. The compressive strength and flexural load of porous polymer concrete with fillers were in the range of $19{\sim}22$ MPa and $18{\sim}24$ KN, respectively. The permeability coefficients of porous polymer concrete with fillers were in the range of $5.5{\times}10^{-1}{\sim}9.7{\times}10^{-2}$ cm/s. At the sulfuric acid resistance, the weight reduction ratios of porous polymer concrete immersed during 8-week in 5% $H_{2}SO_{4}$ were in the range of $1.08{\sim}3.56%$.

• 한국축산식품학회지
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• 제39권6호
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• pp.966-979
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• 2019

Muscle-based by-products are often undervalued although commonly reported having a high amount of natural bioactive peptides. In this study, elastin was isolated from the protein of broiler hen skin while its hydrolysate was prepared using Elastase. Assessment of antioxidative properties of elastin-based hydrolysate (EBH) was based on three different assays; 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical, 2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical and metal chelating ability. The EBH was purified further using ultrafiltration, gel filtration and Reverse- Phase High-Performance Liquid Chromatography (RP-HPLC). The IC₅₀ of ABTS radical activities for EBH were decreased as EBH further purified using ultrafiltration (EBH III; 0.66 mg/mL)>gel filtration (EB-II; 0.42 mg/mL)>RP-HPLC (EB-II4; 0.12 mg/mL). The sequential identification of the peptide was done by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/ TOF-MS) of the potent fractions obtained from RP-HPLC (EB-II4). The presence of hydrophobic amino acids (Val and Pro) in the peptide sequences could potentially contribute to the high antioxidant activity of EBH. The sequences GAHTGPRKPFKPR, GMPGFDVR and ADASVLPK were identified as antioxidant peptides. In conclusion, the antioxidative potential from poultry skin specifically from elastin is evident and can be explored to be used in many applications such as health and pharmaceutical purposes.

• 수산경영론집
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• 제49권1호
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• pp.17-35
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• 2018

This study is to evaluate economic value of the ecosystem service benefits of the marine forests provided to our society on the basis of the global standard valuation manual. The main results of this study are summarized as follows: First, the ecosystem service benefits of marine forests are worth 771,121,551 won per hectare for a year in Korea. Second, when evaluating value based on the services benefits of the marine forests, as resource provisioning service, the annual value of the fishery potential production amounts to 58,512,271 won per hectare. The annual value of the environmental and regulatory services is estimated to total 29,574,000 won per hectare including 546,488 won per hectare for climate regulation, 85,342 won per hectare for pollution purification, and 28,942,170 won per hectare for erosion protection. The annual value of the culture and tourinsm services is estimated to total 15,317,647 won per hectare including 5,011,765 won per hectare for skin scuba service and 10,305,882 won per hectare for sea fishing. Also, the aunnual non-use value is estimate to 637,800,000 won per hectare. Third, assuming that the value of the sea forests increases proportionally to the unit area, the marine forests can be valued at 12.7 trillion won per year based on 12,208.2 hectares of marine forests creation area and 4,272.6 hectares of natural seaweed beds. Fourth, the total economic value of the marine forests can be estimated at 244 trillion won in 2016, if the value of the marine forests permanently continues in the future by applying 5.5% of the social discount rate. The results of this study are expected to serve as a valuable information for improving awareness of the value of marine forests ecosystem and ensuring the validity of the marine forests creation policy by converting the value of the marine forests's ecosystem service into monetary units.