• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.04a
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• pp.158-158
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• 2003

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1557-1564
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• 2009

Human CDX2 is known as a caudal-related homeodomain transcription factor that is expressed in the intestinal epithelium and is important in differentiation and maintenance of the intestinal epithelial cells. The caudal-related homeobox proteins bind DNA according to a helix-turn-helix structure, thereby increasing the structural stability of DNA. A cancer-tumor suppressor role for Cdx2 has been shown by a decrease in the level of the expression of Cdx2 in colorectal cancer, but the mechanism of transcriptional regulation has not been examined at the molecular level. We developed a large-scale system for expression of the recombinant, novel CDX2, in Escherichia coli. A highly purified and soluble CDX2 protein was obtained in E. coli strain BL21(DE3)RIL and a hexahistidine fusion system using Ni-NTA affinity column, anion exchange, and gel filtration chromatographies. The identity and secondary structure of the purified CDX2 protein were confirmed by MALDI-TOF MS, Western blot, and a circular dichroism analyses. In addition, we studied the DNA-binding activity of recombinant CDX2 by ELISA experiment and isolated human CDX2-binding proteins derived from rat cells by an immobilized GST-fusion method. Three CDX2-binding proteins were found in the gastric tissue, and those proteins were identified to the homeobox protein Hox-D8, LIM homeobox protein 6, and SMC1L1 protein.

• Journal of the Korean Society of Industry Convergence
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• v.27 no.2_2
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• pp.475-485
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• 2024

In many regions of Korea, including the Four Major Rivers, the seriousness of the problem of algal blooms due to eutrophication of water quality is being raised.In this study, in order to solve these social problems, we manufactured a filter using natural mineral fusion (red illite, zeolite, germanium ceramic, selenium ceramic, carbon ceramic) and independently developed a tank system for green algae experiments to observe and determine the stages of change in water quality. In order to study ways to improve water quality through quantitative analysis, 1 ton of severely polluted green algae water from the Nak dong River region was stored in a water tank and exposed to ultraviolet rays in the same environment as the Nak dong River. Then, the same environment as the Nak dong River was created. The results were derived from a 5-week water quality test. The results of this experiment confirmed that green-colored cyano bacteria were significantly reduced just by the turbidity results. The results were obtained through a request to the Korea Testing & Research Institute located in Changwon-si, Gyeong sang nam-do. CI-(chlorine ion) and NH3-N(ammonia nitrogen) had the effect of saving every week. The device used in this study was made of natural minerals free of heavy metals that are harmless to the human body and nature through long-term consideration and exploration to kill and prevent various strains living in water. Green purification system using natural hydrogen generating mineral filter were effective a non-chemical and physical methods. The results of this study are one way to contribute to the serious problems caused by green algae in many countries, and will contribute to the water quality environment by preventing the waste of environmental resources, improving the health of the people, and increasing the ability to purify environmental water quality at home and abroad.

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.240.1-240
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• 1997

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 2003.08a
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• pp.179.2-179
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• 2003

No Abstract, See Full Text

• YAKHAK HOEJI
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• v.38 no.6
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• pp.687-695
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• 1994

Lectin from mistletoe(Viscum coloratum) fermented by Lactobacillus plantarum for 1,2,3 days were obtained by salt fractionation, gel filtration, anion exchange chromatography and SDS-PAGE, and compared with the lectin from unfermented mistletoe. The new lectin of molecular weight of about 18,500D from fermented mistletoe was identified.

• Archives of Pharmacal Research
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• v.10 no.2
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• pp.115-120
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• 1987

Methanol extract of Ilex pubescens roots prolonged bleeding time threefold, and inhibited the generation of malondialdehyde released during platelet aggregation inducted by thrombin. Through several purification procedures, its saponin, named ilexoside, was proved to be responsible for the antithrombotic activities of the plant. Ilexosides A, -D and -J and 24-carboxypomolic acid showed strong inhibitory activities on platelet aggregation induced by thrombin.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2004.05a
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• pp.129-129
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• 2004

• KIEAE Journal
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• v.5 no.1
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• pp.3-10
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• 2005

Indoor air quality (IAQ) is recognized as a byproduct of industrialization that took place in the 20th century. The great attention to IAQ has been gained since 1980s, and led to numerous research studies that have mainly made in the developed countries. As the development of residential environment in Korea enters in the process of qualitative growth, IAQ has viewed as one of the core issues in the society, and research in relation to IAQ has been implemented until now in the fields of engineering and medical science. It is widely known that the sources and effects of indoor air pollution have been various, and IAQ research with multidisciplinary approaches is required. Nevertheless, few research embedded into a socio-behavioral approach to identifying interventive measures of residents and analyzing their choices is made. The purpose of this study is to examine the use of an interactive measure of residents in apartments that is air purification with phytoncide available in the market, and to investigate their self-reported effects of the air purifying substance on sick building syndrome symptoms. This study utilizes a field experiment design with pre-test and post-test, and the experiment is conducted in a natural field setting. A total of 87 test subjects from 32 households with already sick building syndrome are drawn via internet, and the participation is solely voluntary. The participants receive and use the air purifying substance, and are asked to use it continuously for two weeks and identify their self-reported symptoms of before- and after-use. The research findings indicate that air purification using phytoncide is effective to alleviate their symptoms in relation to sick building syndrome. Also it is noted that the participants report that the use of the air purification causes the positive impacts on their daily life. Further research is needed that compares the group with only humidifier and evaluates the mid-term and long-term use of the air purification and its effects.

• Journal of the Institute of Convergence Signal Processing
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• v.19 no.4
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• pp.167-173
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• 2018

We propose an air purification system that utilizes aerial plant parts and root zone of indoor plants where light is insufficient and air circulation is bad. In order to maximize the air purification effect of the plant, the aerial plant parts illuminates mixed light combining blue and green LED and CRI(Color Rendering Index) LEDs close to natural light, respectively. And the root zone was forcibly circulated by the fan to use the soil as a filter. The indoor air purification system combined with the light source and the fan removed most polluted air in the shortest. In the case of mixed light and CRI LEDs of indoor air purification system, fine dust decreased by 14%, 14.2%, and TVOC(Total volatile organic compounds) decreased by 7.5% and 9.4%, respectively. In the experiment in which the fan was operated for 15 minutes, the TVOC decreased to 97.8%. The photosynthesis of the plant and the use of soil as a filter were able to purify polluted air in a short time. And the fan's temporary operation gave the similar effect of continuous operation.