• 한국수산과학회지
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• 제35권2호
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• pp.115-121
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• 2002

R plasmid의 분포와 새로운 conjugation 방법 개발을 위하여, 한국의 남해안과 동해안의 양식현장의 어류질병관련 세균으로부터 항균제 다제 내성균을 분리하였다. 분리균 134종에서 10균주가 chlorarnphenicol, tetracycline, streptomycin, ampicillin, colistin, nalidixic acid, oxolinic acid, kanamycin 등에 대한 다제내성의 특성을 가지고 있었으며 이중 V. damsela JE1 한 균주는 chloram-phenicol과 tetracycline에 대한 내성전달 특성을 갖는 R plasmid 와 ampicillin과 kanamycin에 대한 유전자를 chromosome 등에 함유하고 있었다. 분리된 어병세균의 다제내성 특성이 R plasmid에 기인하는 것인지의 확인은 다양한 장내세균의 선택배지로 최근 개발된 CC 배지에서 각기 다른 균주들이 나타내는 증식여부 또는 집락 색깔의 차이를 이용하여 donor, recipient 그리고 transconjugant를 구별할 수 있는 방법을 적용하였다. Conjugation의 최적화를 위해 여러 가지 조건을 비교한 결과, V. damsela JE1의 R plasmid의 전달은 filter mating법을 사용하는 것이 broth mating법보다 약 100배 이상의 높은 성공률을 보여 주었으며 이때 사용온도는 30$^{\circ}C$ 이상, mating 시간은 24시간에서 가장 높은 전달 빈도를 나타내었다. R plasmid를 함유한 V. damsela JE1이 분리된 4개월 후, 동일 양식장의 동일 그룹의 넙치 장내세균에서 분리한 다제내성균 Sphingomonas sp. 3균주는 V. damsela JE1에서 분리된 것과 같은 크기의 R plasmid를 함유하고 있었으며, 그 특성 또한 V. damsela JE1의 R plasmid와 마찬가지로 다제 내성 중 Cm과 Tc에 대한 유전자만이 recipient로 전달되어 장내 세균총이 R plasmid의 reservoir로서의 역할을 하고 있음을 확인하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.54-58
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• 2016

In natural conditions, the black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), colonizes in warm temperate regions, and is active in Korea from May through October. Information on black soldier fly rearing, which is affected by seasonal factors in Korea, is limited. This species colonizes and oviposits in a wide variety of decomposing vegetable and animal matter. For mass rearing of black soldier flies, the egg deposition methods are dependent on sunlight and oviposition sites. In this study, we investigated the substrates and oviposition sites preferred by black soldier fly in artificial rearing system. Our results showed that as black soldier flies prefer mating under sunlight, they prefer ovipositing at sites where adequate sunlight and food substrate are available. Further detailed research is required to develop methods for artificially rearing black soldier fly throughout the year in Korea.

• 생명과학회지
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• 제15권1호
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• pp.33-37
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• 2005

조릿대(S. borealis)는 다년생 초본으로 한국, 일녈, 중국의 일부에 제한적으로 분포한다. 이 종은 자가 및 타가수분에 의한 혼합번식 형태를 취한다. 전분 전기영동을 이용한 12개의 대립 유전자좌위에서 종의 교배계를 조사하였다. 교배계에서 타가 및 자가 수분, 그리고 줄기의 변형으로 영양번식이 이루어지는 매우 복잡한 혼합 방식의 교배 양식은 제한된 분포, 고산식생대 등의 환경에서 생존할 수 있는 조릿대의 생존전략과도 관련이 있을 것으로 보인다 또한 일부 자생지 파괴에 대한 유효집단의 감소가 일어나 생물자원 확보를 위한 자생지 보존이 필요하다

• 농업과학연구
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• 제48권2호
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• pp.191-199
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• 2021

Salmon oil (SO) could be used as a great source of ω-3 fatty acids in pig diet. The purpose of the study was to investigate the SO effect on production performance of sows and their offspring. 48 lactating sows (Landrace × Yorkshire) from Dankook University experimental farm were used in this study. Sows were conceived using either guided natural mating or by artificial insemination. Pregnancy diagnosis was confirmed by ultrasonography on day 25 after mating. Sows and their offspring were assigned randomly to 1 of 2 treatments. Treatments included: 1), control (CON); 2), control + SO 0.5% (CS). The inclusion of SO did not affect (p > 0.05) the litter size throughout the experiment. No difference (p > 0.05) was observed on the average daily feed intake (ADFI), chest circumference, estrus interval and sow backfat thickness between CON and SO treatments. Dietary SO supplemented diet reduced (p < 0.05) body weight loss during lactation compared with control treatment. Pigs fed SO supplemented diet did not affect (p > 0.05) the fecal scores during lactation compared with those fed control treatments. Sows fed SO supplemented diet led to a higher (p < 0.05) initial weight, weaning weight and average daily gain of piglets than those fed control diets. No difference (p > 0.05) was observed on piglet survival and fecal scores throughout the experiment. Inclusion of SO could reduce the body weight loss of sows and improves piglet growth during lactation, indicating SO has beneficial effects for pigs.

• The Korean Journal of Ecology
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• 제19권5호
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• pp.477-486
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• 1996

The freshwater goby, Micropercops swinhonis ($G\"{u}nther$) was studied on the spawning behavior and egg develeopment at the two areas of Chollabukdo, Korea from March to May 1995. Egg mass attached to a layer under stones and the inner wall of waterplant, Cyperus. Spawning behavior was divided in to 1) premating stage: selecting spawning sites and formation of territory by male, 2) mating stage: enticement with zigzag-like dance and intermittant shaking of head by male and fertilization, 3) postmating stage: fanning, guarding and cleaning the nest by male. The eggs were transparent and spherical in shape, measuring $0.9\times1.4mm$ with yellowish yolk sac and many oil globules. Hatching began in about ten days after fertilization at water temperature $16~20^{\circ}C$. The newly hatched larvae were 3.8~4.0 mm in total length with 30 myomeres and their mouths and anuses were opened. Melanophores appeared at the air-bladder and the ventral side of caudal region.gion.

• Journal of Animal Science and Technology
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• 제45권6호
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• pp.917-922
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• 2003

This study was conducted to investigate effects of farm, type of sow Landrace${\times}$Yorkshire, LY; Yorkshire${\times}$ Landrace, YL; Yorkshire${\times}$Yorkshire, YY and multi-cross bred sow, MBS), parity, farrowing year, farrowing season and mating method on reproductive traits such as total number born per litter, number of born alive per litter, number of weaned per litter, number of mummified per litter, number of stillbirth. The Reproductive Records of 3,387 litters from January, 1999 to September, 2002 were obtained from four pig farms managed by Electronic Data Processing(EDP) system. Reproductive performances for two types of F1 Sows(YL and LY) were estimated as 11.34${\pm}$0.266 and 11.57${\pm}$0.263 heads for total number of born per litter, 10.56${\pm}$0.216 and 11.81${\pm}$0.251 heads for number of born alive per litter, and 10.05${\pm}$0.131 and 9.96${\pm}$0.153 heads for number of weaned per litter, respectively. These records are significantly higher(P〈0.05) than those of YY and MBS. However, number of mummified per litter, number of stillborn per litter, number of dead by diarrhea per litter, number of dead by other reasons during lactating period per litter and estrus interval did not show significant difference between types of sows. There were more total number of born per litter and number of weaned per litter in year 2001 than other year. As year passed, number of dead by crush per litter, number of dead by diarrhea per litter and number of dead by other reasons during lactating period per litter reduced from 0.18${\pm}$0.023 to 0.07${\pm}$0.022 head, 0.12${\pm}$0.21 to 0.02${\pm}$0.020 head and 0.43${\pm}$0.041 to 0.22${\pm}$0.040 head, respectively and weaning rate increased from 0.94${\pm}$0.005% to 0.97${\pm}$0.005%. The total number of born per litter and number of born alive per litter were higher(P〈0.05) in the case of mating twice than mating once. The number of weaned per litter, number of mummified per litter and number of stillborn per litter were not significant(P〈0.05) between mating methods. Estrus interval was shorter on the occasion of twice artificial insemination(5.24${\pm}$0.153days) than twice natural mating(6.51${\pm}$0.466days).

• Animal Bioscience
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• 제34권5호
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• pp.844-854
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• 2021

Objective: The potential of extra virgin olive oil (EVOO), betaine (BET), and ginger (GIN), as natural antioxidants, in reducing negative effects of heat stress on physiological responses, antioxidant capacity, semen quality and fertility of bucks under heat stress were investigated. Methods: Forty adult Animal Production Research Institute line rabbit bucks were distributed randomly into four experimental treatments of ten rabbits each. The first treatment was fed the commercial pellet diet (CPD) without supplementation and served as a control. The other three treatments were fed CPD supplemented with EVOO (300 mg), BET (1,000 mg), and GIN (200 mg) per kg diet for 3 consecutive months during the summer season. Results: Supplementation of EVOO, BET, or GIN improved (p<0.05) the sexual desire, progressive motility, vitality, intact acrosome and membrane integrity, sperm cell concentration, sperm outputs and fertility. Seminal plasma total proteins, globulin, total antioxidant capacity, glutathione and glutathione S-transferase, and initial fructose increased (p<0.05), while total lipids, aspartate and alanine aminotransferases and malondialdehyde decreased (p<0.05) compared with the control. In comparing the natural antioxidants treatments, GIN evoked the largest improvement. Conclusion: The inclusion of GIN (200 mg/kg diet) appeared to improve the sexual desire, semen quality and oxidative stress of bucks. This may be a beneficial supplement for the management of rabbit bucks used in natural mating or artificial insemination.

• 한국어류학회지
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• 제33권1호
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• pp.8-14
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• 2021

멸종위기 담수어류 모래주사의 산란행동을 조사하기 위해 산란시기인 2012년 4월부터 5월까지 전북 임실군의 섬진강 일대에서 연구를 실시하였다. 모래주사의 산란은 무리산란 (group spawning)하는 어류와 달리 암수 한 쌍에 의해 이루어졌으며, 수중으로 대량의 생식세포를 방출하여 산란하는 원시적인 생식모드 (broadcast spawner)로 확인되었다. 자연에서 모래주사의 산란행동은 휴식(resting), 유혹(male chase), 자극(body beating), 평행유영(parallel swim), 암컷의 산란회피(female withdrawal), 경쟁(male competition), 산란(spawning), 산란 후 난을 보호하는 행동은 보이지 않아(not guard) 총 8가지 행동을 보인 반면 수조 내에서는 휴식, 유혹, 자극, 평행유영, 산란, 산란 후 난을 보호하지 않은 6가지 행동이 관찰되었다. 특히 산란전 행동은 수조 내에서 보다 자연상태에서 더 빈번하게 일어났다. 이와 같은 행동적 차이는 작고 좁은 수조에서의 행동적 제약에 의한 결과로 추정된다.

• 한국균학회지
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• 제25권2호통권81호
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• pp.111-120
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• 1997

팽나무버섯[Flammulina velutipes(Curt. ex Fr.) Singer]의 새로운 우량균주를 육성하고자 단핵균주를 분리하여 이들의 특성을 조사하고 계통내 교배 시험한 결과 백색 재배종에서 분리된 단핵균주는 모균주에 비해 균사생장의 변이가 적고, 균총의 색이 모두 백색이며 RAPD 밴드로 본 변이가 적었던 반면, 갈색 자생종에서 분리된 단핵균주는 균총의 색이 백색, 갈색, 연갈색으로 분리되고 RAPD 밴드에 다양한 변이를 보였다. 또한 백색 재배종의 교배형은 A1A2B1B2 교배형이었으나, 갈색 야생종의 교배형은 백색종과 교배형이 다르게 나타나 A3A4B3B4교배형으로 결정하였다. 백색 재배종에서 분리한 단핵균주간 자식교배에 의하여 육성한 이핵균주는 모본보다 자실체 수량이 적고 수량의 변이 폭이 컸다. 갈색 자생종에서 분리한 단핵균주를 서로 교배한 결과 자실체의 색깔에 변이를 나타내었는데 백색 자실체를 형성하는 교배균주 중에는 모본보다 수량이 많은 이핵균주도 있었다. 따라서 갈색 자생종의 자식교배로 현재 재배되는 순백계와는 다른 새로운 백색계통을 육성할 수 있었다.

• 복식
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• 제50권5호
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• pp.131-142
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• 2000

The philological consideration of Korean Buddhist Monk's robe and its reviving have been investigated in traditional way. The configuration and colour of Korean Buddhist monk's robes have been properly adapted for an period, territory, and atmospheric phenomena. In case of Korea, introduced Buddhism from China realize modern robe from under the influence of fusion of Chinese ornament culture and Korean traditional ornament culture. As a result of this modern robe has been consisted of a Buddhist monk's robe and a cope. The modem robes has been significantly affected by industrialization, contrary to ancient time taxed robes as public imposts. At present be familiar in mass production we have a preference for the easier way in making and even color forming. In this paper it is focused on dyeing part, which recognized its important in latest time, at first declined according to appearance of chemical dye, evaluated its convenience in use. That is, it is increased natural dye, people begin with recognition of difference of physical properties for dress dyed with chemical pigment and that with natural pigment. In consequence, I have presented that both making colour of gray using traditional method, and three demolished-color prescribed by Buddhist law. Of course, though it become generally know that dyeing of robes occupied significant part of traditional natural dyeing. But in case of religion, it also is important to know symbolical meaning involved in its colour. Most of Buddhism-believers or even Buddhists who actually be dress in robe are without knowing the meaning of colour, or its revealing method. There, I have considered mating three colour of demolished- color included blue, black, and red, and also represented dyeing method of Buddhist monk's robe using both charcoal and ink-spick. From religious font of view, as robes contains involving of blessing, I have an intention of improvement way in dyeing and succession and development of traditional culture through consideration of traditional dyeing method. And I have also intended to restoration the value of robe's colour, disappeared by the time. moreover, I have shown that dyeing with natural pigment is profitable for both environment and sanitary aspect.