• Journal of the Korean Magnetic Resonance Society
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• v.25 no.1
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• pp.1-7
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• 2021

Employing of 13C stable-isotopes in NMR metabolomics can give unique splitting patterns and coupling constants information originated from 13C-13C coupling interaction that provide an important structural information regarding the cellular metabolic process. But it has been known that an undesirable signal distortion in 2D heteronuclear correlation study, due to 13C-13C interaction, hampers an analysis of the coupling information. Recently, we proposed J-scaled distortion-free heteronuclear single-quantum coherence (HSQC) sequence which provides a distortion-free 13C-13C coupling information with a selective resolution enhancement of JCC splitting. In this paper, we dicuss theoretical aspect and practical feature of J-scaled HSQC pulse sequence. The conceptual explanation of orgin of the signal distortion by 13C-13C coupling interaction and design of J-scaled HSQC through exemplified results are provided in brief.

• Structural Engineering and Mechanics
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• v.82 no.2
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• pp.191-204
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• 2022

A three-mass vehicle model including one rigid mass and two unsprung masses is adopted to predict the vehicle-bridge interaction (VBI) and to establish the nonlinear coupled governing equations. To overcome the numerical instability and large computation problems concerning the vehicle-bridge system, the perturbation method is used to convert the nonlinear coupled governing equations into a set of linear uncoupled equations. Formulas for bridge's natural frequencies considering both the VBI and the dynamic responses of bridge and vehicle are proposed. Compared with the numerical results obtained by the Newmark-β method, the theoretical solutions for natural frequencies and dynamic responses are validated. The effects of the important factors of unsprung mass, vehicle damping, surface irregularity on the natural frequencies and dynamic responses of bridge and vehicle are discussed, based on the theoretical solutions.

• Journal of the Korean Magnetics Society
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• v.21 no.5
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• pp.151-156
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• 2011

The influence of thickness on magnetic interaction and magnetic properties in electrodeposited CoPt magnetic films was investigated from the analysis of the magnetic remanence curves and the magnetic hysteresis loops. As the thickness of the CoPt film is increased, the perpendicular coercivity and the saturation magnetization are increased but the squareness is considerably decreased. The analysis results of the magnetic remanence curves and the magnetic hysteresis loops exhibited that the dipolar interaction is the main interaction mechanism for all samples, but the strength of the dipolar interaction gradually increased with increasing sample thickness.

• Steel and Composite Structures
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• v.21 no.1
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• pp.195-216
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• 2016

The dynamic characteristics of continuous steel-concrete composite beams considering the effect of interlayer slip were investigated based on Euler Bernoulli's beam theory. A simplified calculation model was presented, in which the Mode Stiffness Matrix (MSM) was developed. The natural frequencies and modes of partial-interaction composite continuous beams can be calculated accurately and easily by the use of MSM. Proceeding from the present method, the natural frequencies of two-span steel-concrete composite continuous beams with different span-ratios (0.53, 0.73, 0.85, 1) and different shear connection stiffnesses on the interface are calculated. The influence pattern of interfacial stiffness on bending vibration frequency was found. With the decrease of shear connection stiffness on the interface, the flexural vibration frequencies decrease obviously. And the influence on low order modes is more obvious while the reduction degree of high order is more sizeable. The real natural frequencies of partial-interaction continuous beams commonly used could have a 20% to 40% reduction compared with the fully-interaction ones. Furthermore, the reduction-ratios of natural frequencies for different span-ratios two-span composite beams with uniform shear connection stiffnesses are totally the same. The span-ratio mainly impacts on the mode shape. Four kinds of shear connection stiffnesses of steel-concrete composite continuous beams are calculated and compared with the experimental data and the FEM results. The calculated results using the proposed method agree well with the experimental and FEM ones on the low order modes which mainly determine the vibration properties.

• Journal of the Korean GEO-environmental Society
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• v.10 no.6
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• pp.135-141
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• 2009

Fracturing technique using fluid injection into the borehole is widely used technology in the industry for the geothermal heat, oil, and gas extraction. Dealing with fluid-driven natural fractures such as dike and vein indirectly, design technology would be improved by adapting their principles. In this paper, mechanical interaction between the segments is evaluated by modeling highly segmented and closely spaced fluid-driven natural fractures. The number of segments is 71 with 3,339 measured apertures in which the interaction is considerably predicted. To evaluate mechanical interaction, boundary collocation method is used and the net pressure is calculated by using least square method to fit measured apertures. As a result, in case that mechanical interaction is considered, two pressures as fitting parameters are sufficient to capture measured apertures.

• Journal of the Korean Society of Safety
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• v.33 no.1
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• pp.81-87
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• 2018

The seismic response, the natural frequencies and the mode shapes of an offshore wind turbine with twisted tripod substructure subject to various pile-ground interactions are discussed in this paper. The acceleration responses of the tower head by four historical earthquakes are presented as the seismic response, while the other loads are assumed as ambient loads. For the pile-ground interactions, the fixed, linear and nonlinear models are employed to simulate the interactions and the p-y, t-z and Q-z curves are utilized for the linear and nonlinear models. The curves are designed for stiff, medium and soft clays, and thus, the seven types of the pile-ground interactions are used to compare the seismic response, the acceleration of the tower head. The mode shapes are similar to each other for all types of pile-ground interactions. The natural frequencies, however, are almost same for the three clay types of the linear model, while the natural frequency of the fixed support model is quite different from that of the linear interaction model. The wind turbine with the fixed support model has the biggest magnitude of acceleration. In addition, the nonlinear model is more sensitive to the stiffness of clay than the linear pile-ground interaction model.

• Korean Journal of Computational Design and Engineering
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• v.20 no.4
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• pp.337-347
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• 2015

This paper presents a dynamic association and natural interaction method for multi-displays composed of smart devices. Users can intuitively associate relations among smart devices by shake gestures, flexibly modify the layout of the display by tilt gestures, and naturally interact with the multi-display by multi-touch interactions. First of all, users shake their smart devices to create and bind a group for a multi-display with a matrix configuration in an ad-hoc and collaborative situation. After the creation of the group, if needed, their display layout can be flexibly changed by tilt gestures that move the tilted device to the nearest vacant cell in the matrix configuration. During the tilt gestures, the system automatically modifies the relation, view, and configuration of the multi-display. Finally, users can interact with the multi-display through multi-touch interactions just as they interact with a single large display. Furthermore, depending on the context or role, synchronous or asynchronous mode is available to them for providing a split view or another UI. We will show the effectiveness and advantages of the proposed approach by demonstrating implementation results and evaluating the method by the usability study.

• BMB Reports
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• v.43 no.3
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• pp.205-211
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• 2010

Sprouty (Spry) proteins have previously been suggested as negative regulators of the MAPK pathway through interaction with Raf-1. However, the molecular basis of this inhibition has not been elucidated. In this study, we used cells expressing FLAGtagged Raf-1 with point mutations at known phosphorylation sites to reveal that activation of Raf-1 mutants does not correlate with their degree of interaction with Spry2. The association of Raf-1 with Spry2 in intact cells was further corroborated by immunofluorescence colocalization. Additionally, there was no significant change observed in the strength of interaction between Raf-1 mutants and Spry2 after paclitaxel treatment despite differences in the activation levels of these mutants. Thus, our study provides the evidence that Spry2 does not directly regulate Raf-1 kinase activity, but instead acts as a scaffolding protein that assists interactions between Raf-1 kinase and its direct regulators.

• Journal of Power System Engineering
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• v.9 no.4
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• pp.71-76
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• 2005

Marine structures are often in contact with inner or outer fluid as stern, ballast and oil tanks. The effect of interaction between fluid and structure has to be taken into consideration when we estimate the dynamic response of the structure appropriately. Fatigue damages can also be sometimes observed in these tanks which seem to be caused by resonance. Thin walled tank structures in ships which are in contact with water and located near engine or propeller where vibration characteristics are strongly affected by the added mass of containing water. Therefore it is essentially important to estimate the added mass effect to predict vibration characteristics of tank structures. But it is difficult to estimate exactly the magnitude of the added mass because this is a fluid-structure interaction problem and is affected by the free surface, vibration modes of structural panels and the depth of water. I have developed a numerical tool of vibration analysis of 3-dimensional tank structure using finite elements for plates and boundary elements for fluid region. In the present study, the effect of added mass of containing water, the effect of structural constraint between panels on the vibration characteristics are investigated numerically and discussed. Especially a natural frequencies by the fluid interaction between 2 panels and a breath mode of the water tank are focused on.

• Bulletin of the Korean Chemical Society
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• v.31 no.11
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• pp.3241-3246
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• 2010

NHERF ($Na^+/H^+$ exchanger regulatory factor) and E3KARP (NHE3 kinase A regulatory protein) play important roles in membrane targeting, trafficking and sorting of ion channels, transmembrane receptors and signaling proteins in many tissues. Each of these proteins contains two PDZ (PSD-95/Dlg-1/ZO-1) domains, which mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes. The interaction between NHERF and E3KARP was investigated by surface plasmon resonance spectroscopy (BIAcore), fluorescence measurement, His-tagged pull-down experiment, and size-exclusion column (SEC) chromatography. BIAcore experiments revealed that NHERF bound to E3KARP with an apparent $K_D$ of 7 nM. Fluorescence emission spectra of the NHERF-E3KARP complex suggested that the tight interaction between these proteins was accompanied by significant conformational changes in one or both. The CD spectra of NHERF and E3KARP show that the conformational changes of these proteins were dependent on pH and temperature. These results implicate that the NHERF-E3KARP complex allows intracellular signaling complexes to form through PDZ-PDZ interactions.