• Bulletin of the Korean Chemical Society
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• 제25권11호
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• pp.1714-1716
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• 2004

• 식물병연구
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• 제19권4호
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• pp.281-287
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• 2013

2006년에서 2007년까지 콩연구센터 육종포장 주변에서 잡초 서식상황을 조사한 결과 31과 74속 96종의 식물이 확인되었다. SYMMV의 자연기주 또는 중간기주를 동정하기 위하여 26과 84종의 식물체가 포함되는 495점의 시료를 RT-PC R로 분석한 결과, 단지 콩과 식물에서(돌콩, 새팥, 토끼풀, 비수리)만 SYMMV가 검출되었다. SYMMV를 가지고 있는 콩과 식물 중에서 분석한 돌콩의 3분의 1이상이 SYMMV를 보독하고 있는 것으로 보아 돌콩은 SYMMV의 감염원으로써 중요한 역할을 담당하는 것으로 생각된다. 돌콩은 SYMMV에 7월 중순경에 감염되는 것으로 추정되었다. 돌콩에서 SYMMV의 빠른 감염시기, 높은 종자감염율 그리고 종자전염과 같은 결과로 볼 때, 돌콩은 SYMMV의 생활사 완성에 큰 역할을 담당하고 있는 것으로 판단된다.

• The Plant Pathology Journal
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• 제36권1호
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• pp.67-75
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• 2020

Tomato spotted wilt virus (TSWV) is one of the plant viruses transmitted by thrips and causes severe economic damage to various crops. From 2008 to 2011, to identify natural host species of TSWV in South Korea, weeds and crops were collected from 5 regions (Seosan, Yesan, Yeonggwang, Naju, and Suncheon) where TSWV occurred and were identified as 1,104 samples that belong to 144 species from 40 families. According to reverse transcription-polymerase chain reaction, TSWV was detected from 73 samples from 23 crop species, 5 of which belonged to family Solanaceae. Additionally, 42 weed species were confirmed as natural hosts of TSWV with three different life cycles, indicating that these weed species could play an important role as virus reservoirs during no cultivation periods of crops. This study provides up-to-date comprehensive information for TSWV natural hosts in South Korea.

• Parasites, Hosts and Diseases
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• 제23권2호
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• pp.197-202
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• 1985

Family Heterophyidae에 속하는 흡충류 3종의 성충을 고양이에서 검출함으로써 자연감염을 확인하였다. 1983년 4월 8일부터 1985년 4월 9일 사이에 서을 중앙시장에서 구입한 고양이 181마리의 소장 및 내부장기를 부검한 결과 3마리의 고양이에서 Heterophyopsis continua Onji et Nishio, 1916 성충 5마리 (1∼3마리), 7마리의 고양이에서 Pygidiopsis summa Onji et Nishio, 1916 성충 223마리 (1∼200마리), 3마리의 고양이에서 Heterophyes heterophyes nocens Onji et Nishio, 1916 성충 26마리 (7∼11마리)를 각각 얻을 수 있었다. 이들 중 H. heterophyes nocens를 제외한 2종의 이형흡충류 H. continua 및 P. summa는 국내에서 고양이의 자연감염으로 처음 보고되는 것이다.

• Parasites, Hosts and Diseases
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• 제59권1호
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• pp.1-8
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• 2021

Leptotrombidium scutellare is one of the 6 main vectors of scrub typhus in China. It has been found in more than 15 provinces of China. Especially in Yunnan, it was found to be mainly distributed in some mountainous areas with high altitude, low temperature and low precipitation. Rodents and some other small mammals were the most common hosts of L. scutellare. To date, more than 40 host species of L. scutellare have been recorded with very low host specificity, and the main hosts varied in different geographical regions. L. scutellare had a strong resistance against the cold environment, and the temperature and humidity were 2 important factors affecting its growth and development. Among different individuals of their rodent hosts, L. scutellare mites often showed an aggregated distribution pattern, which reflected the interspecific cooperation of the mites. The chromosome karyotype of L. scutellare was 2n=16 and all the 8 pairs of chromosomes were short rod-shaped with metacentric or sub-metacentric types. The isozyme spectrum supported that L. scutellare, L. deliense and L. rubellum were in the same species group. Based on the natural infection, experimental transmission and epidemiological evidence, L. scutellare has been eventually confirmed as the second major vector of scrub typhus in China, which is second only to L. deliense.

• 한국자기공명학회논문지
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• 제24권4호
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• pp.123-128
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• 2020

All plants in natural ecosystems are living in symbiosis with endophytes. Recently, there has been an increasing interest in endophytes since these organisms can interact with the hosts and produce various structurally or biologically interesting molecules. This study aimed to identify these molecules from endophytes. Chemical investigation of Climacium dendroides-derived fungus Talaromyces sp. resulted in the isolation of two diphenyl ether derivatives, purpactin A (1) and penicillide (2), and two steroids, dankasterone A (3) and calvasterol B (4). The structures of the compounds were identified via extensive spectroscopic and spectrometric methods. Four compounds did not show any antioxidative activities in the on-line antioxidant activity screening system.

• 식물병연구
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• 제10권2호
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• pp.130-134
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• 2004

다른 기주에 발생하는 탄저병(C. gloeosporioides)이 딸기에 전염되는지를 알아보기 위해 사과, 고추, 대추, 감의 이병된 과실에서 탄저병균을 분리동정하고 딸기에서의 병원성을 비교하였다. 각 기주별로 분리된 C. gloeosporioides의 배양적 특성과 포자의 크기는 서로 유사하였으나 말기에 접종을 하였을 경우에는 감에서 분리된 균주가 병원성이 가장 강하였고, 대추에서 분리된 균주는 약한 병원성을 보였다. 각 기주별로 병원성을 나타내는 균주를 선발하여 포장에서 병원성을 검정하였을 때 모두 탄저병이 발병되었으므로 다른 기주에서 발생한 탄저병도 자연상태에서 딸기로 전염이 가능한 것을 확인할 수 있었다.

• Animal cells and systems
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• 제15권4호
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• pp.315-324
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• 2011

The actiniarian sea anemone, Entacmaea quadricolor, and the scleractinian coral, Alveopora japonica, host symbiotic dinoflagellates belonging to the genus Symbiodinium (Freudenthal). We studied the host-symbiont specificity of these two anthozoan hosts in the northwestern Pacific Ocean. Symbionts within the two hosts were identified using partial large subunit (LSU) ribosomal DNA (rDNA) and complete internal transcribed spacers (ITS) 1 rDNA regions. The host, E. quadricolor, was identified using the partial LSU rDNA molecular marker. Genetic analysis showed that E. quadricolor only harbors dinoflagellates belonging to subclade C1/3 of the genus Symbiodinium. Moreover, no genetic variation was detected among the symbionts of E. quadricolor within the study region (Korea and Japan), even though the two distant sites were separated by more than 1000 km, at collection depths of 1 m in shallow and 13-16 m in deep water. Whilst scleractinian corals host multiple Symbiodinium clades in tropical waters, A. japonica, sampled over a wide geographical range (800 km) within the study region, only hosts Symbiodinium sp. clade F3. The high specificity of endosymbionts in E. quadricolor and A. japonica within the northwestern Pacific Ocean could be accounted for because symbiotic dinoflagellates within the host anemones appear to be acquired maternally, and the Kuroshio Current might affect the marine biota of the northwestern Pacific. However, the consistency of the symbiotic relationships between these two anthozoan hosts and their endosymbionts could change after climate change, so this symbiotic specificity should be monitored.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.122-123
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• 2003

Apple stem grooving virus (ASGV) belongs to Capillovirus and infects pome fruits. Transmission mode of ASGV is known by grafting and mechanical inoculation into susceptible hosts, not by any other natural vectors. But we have observed the spread of ASGV in the field without mechanical inoculation or grafting. Transmission seems to be occurred from tree-to-tree and tree-to-susceptible herbaceous plants along but not across ditches in the field. In order to ascertain this possibility, various fungi were isolated and cultured from ASGV-infected plants and 69 isolates were characterized. By means of RNA dot-blot hybridization and PCR analysis, 3 isolates were sorted out for further studies. The isolates were identified to Tataromyces sp. and belonged to Phenicillium by morphological characteristics and molecular markers. As an experimental host, 10 kidney beans (Phaseolus vulgaris) were screened and Kyunggi-5 was selected for virus amplification and symptom development. Kyunggj-5 infected by fungi which seemed to carry ASGV showed the typical disease symptoms and viral coat protein genes were detected from all tested plants. To confirm the Koch's rule, fungi cultured from inoculation origins of kidney bean were grown on PDA media and re-inoculated to hosts. The fungi isolated from inoculation origins induced the typical disease symptoms on hosts. However virus free fungi did not induce any symptom on the experimental hosts. This bioassay showed that these typical symptoms were caused by virus, not fungi.

• 한국미생물·생명공학회지
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• 제50권2호
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• pp.240-244
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• 2022

Precisely reliable and quantitative reporters can provide phenotypes that are consistent with research goals in protein expression. Here, we developed an improved reporter mATglu III 5 by directed evolution using a versatile β-glucosidase ATglu derived from Agrobacterium tumefaciens. When expressed in hosts, a vector containing this mutant distinctly showed a colored or fluorescent phenotype, according to the supplemented substrate, without any inducer. Analysis of mATglu III 5 showed it to be fully functional in fusion state with oligomeric proteins, especially under non-induction conditions, thereby offering an alternative to conventional reporters.