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http://dx.doi.org/10.48022/mbl.2203.03008

Directed Evolution of a β-Glucosidase for Improved Functions as a Reporter in Protein Expression  

Lim, Ho-Dong (Department of Biological Sciences and Research Center of Ecomimetics, College of Natural Sciences, Chonnam National University)
Han, So-Young (Department of Biological Sciences and Research Center of Ecomimetics, College of Natural Sciences, Chonnam National University)
Park, Gi-Hye (Department of Biological Sciences and Research Center of Ecomimetics, College of Natural Sciences, Chonnam National University)
Cheong, Dae-Eun (Department of Biological Sciences and Research Center of Ecomimetics, College of Natural Sciences, Chonnam National University)
Kim, Geun-Joong (Department of Biological Sciences and Research Center of Ecomimetics, College of Natural Sciences, Chonnam National University)
Publication Information
Microbiology and Biotechnology Letters / v.50, no.2, 2022 , pp. 240-244 More about this Journal
Abstract
Precisely reliable and quantitative reporters can provide phenotypes that are consistent with research goals in protein expression. Here, we developed an improved reporter mATglu III 5 by directed evolution using a versatile β-glucosidase ATglu derived from Agrobacterium tumefaciens. When expressed in hosts, a vector containing this mutant distinctly showed a colored or fluorescent phenotype, according to the supplemented substrate, without any inducer. Analysis of mATglu III 5 showed it to be fully functional in fusion state with oligomeric proteins, especially under non-induction conditions, thereby offering an alternative to conventional reporters.
Keywords
Versatile reporter; ${\beta}$-glucosidase; directed evolution; activity staining;
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