• Korean journal of applied entomology
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• v.52 no.4
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• pp.437-448
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• 2013

Hemipteran bugs, which were previously considered as secondary pests, have currently become important pests of numerous crops. Among them, Riptortus pedestris (Fabricius) is a major species that occurs in Korea, Japan, China, and South Asian countries. Riptortus pedestris infests leguminous crops like soybean, vetches, and red clover; fruit trees like persimmon and yuju; and grains like barley, foxtail millet, broomcorn, and sorghum. Riptortus pedestris causes the greatest damage to soybean, as it is the most suitable host for the bug. Feeding damage during pod formation significantly reduces the yield of soybean. Currently, 17 insecticides, including diazinon and etofenprox, are registered for the control of hemipteran bugs in Korea, and growers apply insecticides two to three times on a regular basis. Aggregation pheromone traps are widely used as a monitoring tool and partial control measure. The aggregation pheromone of R. pedestris attracts conspecific adults and nymphs and is used for food exploitation rather than sexual attraction. In addition, the pheromone serves as a kairomone for egg parasitoids such as Gryon japonicum (Ashmead) and Ooencyrtus nezarae Ishii. As a new method of pest management, nonviable host eggs were included in the pheromone trap to catch R. pedestris and propagate parasitoids. As a part of cultural practices, resistant soybean varieties with specific color and size of pod and control of flowering time through the alteration of planting date can be used. For the effective management of R. pedestris in the near future, development of cultural practices that can support natural control factors and the use of multiple control tactics are needed.

• Korean journal of applied entomology
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• v.41 no.3
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• pp.199-204
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• 2002

Population fluctuation of O. kashmirica benefica, a major natural enemy of citrus red mite (Panonychus citri), was surveyed from 1997 to 2000 in yuzu orchards in Goheung region and, developmental periods, developmental threshold temperature (DT) and effective cumulative temperature (ET) were estimated. Under four constant temperatures of 15, 20, 25 and 30$\^{C}$, developmental periods from egg to adult of the beetles were 67.3, 31.4, 19.4 and 15.3 days, respectively. The period of each stage was long in the order of pupa> egg > 3rd larva> 2nd larva> 1st larva. Mean numbers of eggs oviposited by a female per day were 3.3, 5.6, 7.3 and 7.7 under the temperatures, respectively. The highest hatchability (92%) and adult emergence (60%) were shown under 25$\^{C}$. Adult longevity was 54.3 days for females, and 58.6 days for males. In natural condition, the sex ratio of the beetle was 0.58. Developmental thresholds (DT) for egg, 1st, 2nd, 3rd larva, pupa, and egg to adult were 12.7, 10.0, 10.8, 7.9, 10.1 and 10.6$\^{C}$, respectively. The effective cumulative temperatures (ET) were 41.4, 22.9, 22.7, 46.6, 165.3, 292.9 day-degree at the same development stage, respectively. The beetle occurred from early April to early December in yuzu orchards. The population peak of the beetles was dependent on the amount of prey mite (Panonychus citri), and the ratio of the predator to prey mite was 1 to 100-200 at predator peak stage in the field.

• Korean journal of applied entomology
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• v.45 no.2 s.143
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• pp.195-200
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• 2006

This study was carried out to clarify the biologies and morphological characteristics of Rhynchaenus sanguinipes. Also some chemicals were tested to screen the effective insecticide for the control of the species. Up to date, Zelkova serrata has been hewn as host plant of Rhynchaenus sanguinipes, which shows serious damage in this country. In the present study, Ulmus pumila was first found as host plant in this study. Body lengths of larvae, pupa and adult were 4.53$\pm$0.30 mm, 3.30$\pm$0.42 mm and 2.96$\pm$0.12 mm, respectively. The overwintered adult of the species emerged on early April to late April, and adult of nekt generation emerged on early May to late May, Pupal periods were 10, 7.2, 5.1 and 4 days on 16, 20, 24 and 28$^{\circ}C$, respectively. The lower developmental threshold temperature was 5.8$^{\circ}C$. Four braconid parasitoids were found as natural enemies, which emerged mainly on late April to early June. Insecticidal activities with treatments of fenitrothion 50% EC, indoxacarb 30% WG, ethofenprox 20% EC and thiacloprid 10% SC was investigated against adult of R. sanguinpes, and they showed >90% mortality.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.211-217
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• 2001

Field survey for Orius spp., predacious natural enemies of thrips pests, on several plants by beating or sweeping method revealed that O. sauteri, O. strigicollis, O. minutus, and O. nagaii habituated in open fields or in greenhouses of Korea. Among them, O. sauteri was the most dominant species at all areas (83.0~92.5%), followed by O. strigicollis and O. minutus. Especially, O. sauteri exclusively dominated on the economically important crops such as soybean, red pepper, and watermelon. On the other hand, O. strigicallis and O. nagaii occurred mainly on ornamental plants including chrysanthemum and rose and on rice, respectively, during summer-autumn season. The seasonal occurrence varied depending on the plant. Initial population of Orius spp. was first observed on white clover in late May, followed by on soybean, red pepper, an buck wheat in early June, indcating that movement of Orius spp. relates to the flowering time of the plant. Peak of Orius spp. abundance was found first in July and secondly in August or September. On white clover and buck wheat the first peak of abundance was one week earlier than on soybean and red pepper. Therefore, we suppose that wild plants such as white clover can serve as a reservoir for Orius spp. after overwintering.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.379-386
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• 2021

Asian gypsy moth, Lymantria dispar asiatica (Lepidoptera: Erebidae), which is an indigenous pest in Korea, a large outbreak has been reported in Gyeonggi-do, Chungcheong-do, and northern Gyeongsangbuk-do from 2019 in forest and nearby downtown areas, causing emotional damage to forests and city dwellers. During the indoor culture of gypsy moth eggs collected in Yecheon, Gyeongbuk in 2021 we found that 79.65% (321/403 of first instar larvae) were died due to Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) infection. Sequence analyses of 36 gypsy moths collected from 12 regions in Korea using LdMNPV late expression factor-8 (lef-8) and polyhedrin (polh) genes showed a genetic variation of 0.80% and 0.86%, respectively. Comparison to GenBank data showed that the Korean samples were most similar to LdMNPV in Japan, whereas most different to those of Turkey. These results showed a high infection rate of LdMNPV in Korea and LdMNPV is one of the important population regulators of the gypsy moth.

• Korean Journal of Breeding Science
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• v.42 no.2
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• pp.157-162
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• 2010

This study was carried out to develop of macro-protocol and the biosafety guide for 'Nakdong', and 'Golden Rice' (genetically modified vitamin A rice) by large scale field trial of GM crops. Typically, when a new GM crop is created, breeders should conduct field test to make sure the GM crop is safe, and provide some information on GM crops for approval. A total of isolated 4,700 $m^2$ field for trial of GM crops were prepared, and 'Nakdong' and 'Golden rice' were cultivated by standard method of RDA (Rural Development Administration, Korea). Field studies indicated that the population densities of insect pests and natural enemies have no difference between two varieties. While insect pest density on 'Nakdong' was slightly higher than on Golden Rice, but natural enemy density on Golden Rice was a little higher. These results provided the insect diversity for risk assessment analysis of Golden Rice and suggested that the macro-protocol could be useful to detect GM plants.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.209-218
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• 2019

This study was conducted to develop a technology for environmentally friendly control of sweet-potato whitefly, Bemisia tabaci, by controlling their behavior using a push-pull strategy in a tomato greenhouse. B. tabaci was attracted the most by yellow color, light source of 520 nm, whereas it avoided the complex light treatment of 450 + 660 nm. The two natural enemies of B. tabaci, Cyrtopeltis tenuis and Orius laevigatus, were attracted the most by 520 nm light source. B. tabaci was repelled by the volatile organic compounds ocimene and carvacrol and was the most attracted by methyl isonicotinate. When buckwheat was added into the tomato greenhouse, the density of C. tenuis was maintained at about 16 times higher than when untreated for 15 days. As a result of the combined treatment of push-pull strategy, the density per trap of B. tabaci was three times lower than when no treatment was applied, and the control of this pest increased with time and reached up to 68.7%.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.615-622
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• 2022

In this study, we sought to determine an appropriate LED light source that can be applied for the quality control of Aphidius colemani. Four LED light sources (385, 405, 450, and 660 nm) were evaluated in a Y-tube experiment (choice test) using a 5,000-k white LED as a control. The 385 nm LED light source was found to have attractant effect on A. colemani. In a no-choice test (Y-tube experiment) evaluating the remaining three LED light sources (385, 405, and 450 nm), the attractiveness of these light sources for A. colemani was high in the order 450 nm > 385 nm > 405 nm light, with no statistically significant differences between 450 nm and 385 nm. Given that 385 nm LED illumination may cause skin damage, we selected 450 nm LEDs for application in a device used for the quality control of A. colemani, which we subsequently demonstrated to have an attraction rate of 87.4%. We believe the findings of this study will make a valuable contribution to the quality control of natural enemies.

• Korean journal of applied entomology
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• v.51 no.1
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• pp.47-58
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• 2012

Effect of a new crop protectant 'Bt-Plus' on natural enemies was analyzed in this study. Tested natural enemies included two parasitic species of $Aphidius$ $colemani$ and $Eretmocerus$ $eremicus$, and four predatory species of $Harmonia$ $axyridis$, $Orius$ $laevigatus$, $Amblyseius$ $swirskii$, and $Phytoseiulus$ $persimilis$. 'Bt-Plus' was formulated by combination of three entomopathogenic bacteria ($Xenorhabdus$ $nematophila$ (Xn), $Photorhabdus$ $temperata$ subsp. $temperata$ (Ptt), $Bacillus$ $thuringiensis$ (Bt)) and bacterial metabolite (BM). All three types of 'Bt-Plus' showed significantly higher toxicities against fourth instar $Plutella$ $xylostella$ larvae than Bt single treatment. Two types of bacterial mixtures ('Xn+Bt' and 'Ptt+Bt') showed little toxicity to all natural enemies in both contact and oral feeding assays. However, 'BM+Bt' showed significant toxicities especially to two predatory mites of $A.$ $swirskii$ and $P.$ $persimilis$. The acaricidal effects of different bacterial metabolites were evaluated against two spotted spider mite, $Tetranychus$ $urticae$. All six BM chemicals showed significant acaricidal effects. The BM mixture used to prepare 'Bt-Plus' showed a high acaricidal activity with a median lethal concentration at 218.7 ppm (95% confidence interval: 163.2 - 262.3). These toxic effects of bacterial metabolites were also proved by cytotoxicity test against Sf9 cells. Especially, benzylideneacetone, which was used as a main ingredient of 'BM+Bt', showed high cytotoxicity at its low micromolar concentration.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.283-289
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• 2013

This study was performed to investigate the biological characteristics of the brown lacewing Micromus angulatus (Stephens) at four different temperatures (15, 20, 25, and $30^{\circ}C$) and $70{\pm}10%$ relative humidity under a photoperiodic regime of 16:8(L:D) h. The developmental threshold and effective accumulative temperature from egg to pupa for M. angulatus were $9.6^{\circ}C$ and 270.3 day-degrees, respectively. The developmental period of egg, larva, and pupa at $25^{\circ}C$ was 4.4, 5.5, and 6.9 days. respectively. The longevity of an adult female M. angulatus was 34.9 days. The oviposition period for M. angulatus was 28.7 days, in which it laid a total number of 515.2 eggs during its life span. The maximum number of eggs laid by a female in a day was 54.8. Daily consumption by M. angulatus at $25^{\circ}C$ was 18.9 $1^{st}$ instars, 47.2 $2^{nd}$ instars, 57.7 $3^{rd}$ instars, and 91.0 adults of the glasshouse potato aphid, Aulacorthum solani. Therefore, M. angulatus could be a promising biological control agent against aphids.