• Biomolecules & Therapeutics
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• 제25권6호
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• pp.559-568
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• 2017

Metabolomics has been used as a powerful tool for the analysis and quality assessment of the natural product (NP)-derived medicines. It is increasingly being used in the quality control and standardization of NP-derived medicines because they are composed of hundreds of natural compounds. The most common techniques that are used in metabolomics consist of NMR, GC-MS, and LC-MS in combination with multivariate statistical analyses including principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). Currently, the quality control of the NP-derived medicines is usually conducted using HPLC and is specified by one or two indicators. To create a superior quality control framework and avoid adulterated drugs, it is necessary to be able to determine and establish standards based on multiple ingredients using metabolic profiling and fingerprinting. Therefore, the application of various analytical tools in the quality control of NP-derived medicines forms the major part of this review. $Veregen^{(R)}$ (Medigene AG, Planegg/Martinsried, Germany), which is the first botanical prescription drug approved by US Food and Drug Administration, is reviewed as an example that will hopefully provide future directions and perspectives on metabolomics technologies available for the quality control of NP-derived medicines.

• Journal of Periodontal and Implant Science
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• 제29권1호
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• pp.173-192
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• 1999

The purpose of this study was to evaluate the effects of bioactive glass and natural coral on the human periodontal ligament fibroblast(HPLF) behaviors during the regeneration process of peridontium. To determine the cellular events occuring in the presence of the particles of bioactive glass and natural coral, HPLF were isolated from healthy premolar teeth extracted for orthodontic treatment. Cells were cultured in ${\alpha}$MEM at 37$^{\circ}C$, 5% $CO_2$, 95% humidity incubator. Bioactive glass and natural coral were powdered, and each particles(<40${\mu}$m) were placed on the cultured cells at the concentration of 0.3mg/ml, and 1,0mg/ml for experimental group. In control group no particles were added. And each group was evaluated by examining the cell morphology under phase-contrast micrograph at 4 day and transmission electron micrograph(TEM) and scanning electron micrograph(SEM) at 14 day, alkaline phosphatase activity at 5 and 9 day, protain synthesis at 4 day, DNA synthesis at 1, 2, 3 and 4 day, cell proliferation at 1, 3, 5,7 and 9 day and the formation of bone nodule at 30 day after culturing all groups in mineralizing supplemented mediun, No significant changes in cell morphology by adding these two matirials were found under phase contrast microscopy and TEM. HPLF phagocytocized each particles suggesting that HPLF is involved in the process of resorbing each particles and that bioactive glass were more biocompatible than natural coral. The ALPase activity of bioactive glass 0.3 mg/ml was similar with control groups and all the rests of control groups were significantly low(P<0.01) indicating a transient dedifferentiation of HPLF in the presence of bioactive glass and natural coral particles. There were no significant differences of protein synthesis between all groups. The DNA synthesis in experimental groups were significantly lower than control groups at 1, 2 and 3 day (P<0.01) but became similar to control groups at 4 day. Between control groups, the DNA synthesis in bioactive glass O.3mglml group was significantly higher than other groups(P<0.01). Cell proliferation in natural coral 1.0mg/ml and bioactive glass 1.0mglml groups were significantly lower than control group at 3 day(P<0.05) and there were no differences at 5, 7, 9 day. There were more bone nodule formation in experimental groups than in control groups. In conclusion, these results indicated that bioactive glass and natural coral have some effects of a transient dedifferentiation on HPLF and regeneration of periodontal tissues, however any significant cytotoxic effect on HPLF by these two particles were not found.

• Journal of the Korean Wood Science and Technology
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• 제52권3호
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• pp.262-275
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• 2024

In Indonesia, Coptotermes curvignathus Holmgren and Cryptotermes cyanocephalus Light termite attacks can damage wood and causing losses of 8.7 trillion rupiah per year. Wood treatment and soil barrier are very important to protect structures and their components from termite infestation. The application of synthetic chemicals that pose risks to the environment and human health. The growing movement to replace these chemicals with new termiticides that are safe for the environment in place of persistent organic pollutants. Efficacy performance in entomopathogenic fungi spores such as that produce decanoic acid, hexadecanoic acid, palmitic acid, and octadecadienoic acid are potential compounds that provide for ecofriendly termite control. Entomopathogenic fungal spores from Metarhizium anisopliae, Trichoderma harzianum, and Beauveria bassiana, as the active ingredient were formulated with nanocellulose was added as an inert ingredient to which helped to deliver the active ingredients on controlling the target pest, and enhance the utility ability of the product fungi to control termites. The mortality of these termites successively entomopathogenic fungi was the main cause of death. The higher concentrations being associated with of nanocellulose used affect increased the number of termites mortality. M. anisopliae paired with 60% nanocellulose concentrate was the best percentage for yielded the greatest C. curvignathus and C. cynocephalus termite control. In the field testing, M. anisopliae provided better, it showed the finest result of termite control rather than B. bassiana and T. harzianum. The results of the research indicate that entomopathogenic fungi can be used for Pest Control Management as the subterranean termite and drywood termite control.

• 한국유기농업학회지
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• 제20권2호
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• pp.231-241
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• 2012

천일염의 농업적 활용은 관행농업에서 뿐만 아니라 친환경농산물 생산을 위해 농업인들 사이에서 증가하는 추세이다. 그러나 다량의 천일염을 지속적으로 살포할 경우 토양에 염류가 집적될 소지가 크고, 이로 인해 작물의 생육장해가 초래될 수 있다. 따라서 본 연구는 천일염을 토양에 살포하였을 때의 토양 이화학성과 마늘의 무기성분 함량과 수량에 미치는 영향을 조사하고자 수행하였다. 천일염을 살포하는 양이 증가할수록 토양의 전기전도도, 치환성 나트륨, 염소이온, 황산이온은 표토에서 높아졌다. 그러나, 수확기에는 천일염의 성분이 강우에 의해 심토로 용탈되어 작물이 생육하는 근권에서는 전기전도도, 치환성 나트륨, 염소이온, 황산이온은 감소하였고, 염소이온은 다른 성분보다 더욱 빠르게 심토로 용탈되었다. 토양의 나트륨 흡착비, 치환성 나트륨 백분율, 토양 분산율은 천일염이 투입량이 증가할수록 높아지는 경향이었다. 천일염을 토양에 살포하였을 때 마늘의 무기성분 중에 질소, 칼슘, 마그네슘, 미량원소인 철, 망간, 아연 등의 함량은 줄어들었고, 바닷물에 많은 성분인 염소 성분의 흡수는 증가하였으나, 마늘의 수량에는 큰 영향을 미치지 못하였다.

• 한국임상수의학회지
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• 제16권1호
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• pp.145-149
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• 1999

This study was designed to examine therapeutic effect of natural honeybee (Apis mellifera) venom in pigs with arthritis. Pigs were assigned to treated and nontreated control groups. In treated group, 51 pigs were given natural honeybee venom once a day for 5 consecutive days. Acupoints of Sam-li, Jok-su and Pal-ja were stung by the natural honey bees. In control group, 46 pigs were intramuscularly injected with a standard dosage of penicillin G (400,000 IU/kg of body weight) once a day for 5 consecutive days. On day 5 following bee venom therapy, 73.9% of control pigs and 86.2% of pigs in treated group recovered from arthritis. Bee acupuncture therapy did not show any side effects such as allergy, intoxication, hemorrhage, or infection. It might be concluded that honeybee venom therapy was effective in controlling of pig with arthritis.

• 한국방재학회:학술대회논문집
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• 한국방재학회 2007년도 정기총회 및 학술발표대회
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• pp.94-97
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• 2007

This study aims to find the characteristics of damage and states of natural disasters at Gimhae, Gyeongsangnam-do from 1985 to 2004. Using the data of Statistical yearbook of calamities issued by the National Emergency Management Agency and Annual Climatological Report issued by the Korea Meteorological Administration. we have analyzed the cause, elements, and vulnerable regions for natural disasters. Major causes of natural disaster at Gimhae are four, such as a heavy rain, heavy rain typhoon, typhoon, storm snow, and storm. The cause of disaster recorded the most amount of damage is typhoon. The areas of Hallim-myeon, Sangdong-myeon, and Saengnim-myeon are classified the vulnerable region for the natural disasters in Gimhae. Therefore, it seems necessary to build natural disaster mitigation plan each cause of disaster to control water resources and to reduce damage for these areas.

• 제어로봇시스템학회논문지
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• 제18권11호
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• pp.1059-1064
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• 2012

In this paper, some applications of natural language processing techniques for information retrieval have been introduced, but the results are known not to be satisfied. In order to find the roles of some classical natural language processing techniques in information retrieval and to find which one is better we compared the effects with the various natural language techniques for information retrieval precision, and the experiment results show that basic natural language processing techniques with small calculated consumption and simple implementation help a small for information retrieval. Senior high complexity of natural language processing techniques with high calculated consumption and low precision can not help the information retrieval precision even harmful to it, so the role of natural language understanding may be larger in the question answering system, automatic abstract and information extraction.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 2004년도 춘계학술대회 논문집
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• pp.176-183
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• 2004

• 한국환경보건학회지
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• 제43권2호
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• pp.122-129
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• 2017

Objectives: Most of the $PM_{10}$ in subway stations is spread by the train-induced wind from the tunnels. Therefore, in order to improve air quality in subway stations, it is important to remove the $PM_{10}$ from the tunnels. After the installation of PSD (platform screen doors), the influence of train-induced wind and $PM_{10}$ at the platform has decreased, but is estimated to have increased in subway tunnels. This study was conducted to investigate the control of $PM_{10}$ in subway tunnels by applying a 500-fold diluted solution mixed with a humectant using a natural polymer. Methods: For this purpose, we tested the dust reduction effect in a laboratory and corrosion test and water pollution using fish and aquatic plants for the natural dust collecting agent. In the tunnel of a subway station, we used the natural dust collecting agent over 15 days. The study was carried out on $PM_{10}$ control during operation, which accounts for more than 70% of subway dust. Results: As results, the natural dust collecting agent exhibited an excellent dust control effect, and it was safe for water quality and soil. It showed the effect of controlling $PM_{10}$ in the subway tunnel by 49.5- 64.7% over 15 days. The use of the dust collecting agent for the control of $PM_{10}$ could be confirmed in the subway. Conclusion: It is necessary to clearly explain the major portions of chemical components contained in $PM_{10}$ to figure out the characteristics of $PM_{10}$ and to develop effective reduction measures to decrease the adverse effects of $PM_{10}$ in the subway.

• 한국유기농업학회지
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• 제10권3호
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• pp.79-86
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• 2002

GB$_{10}$, Chitofarm 및 왕겨숯과 왕겨숯 液 등 天然物質을 가을 무 栽培圃場에 種子處理, 土壤處理 및 葉面撒布를 實施하여 收量과 成分分析으로 遂行한 實驗 成績은 다음과 같다. 1. 무 生育特性 중 株重, 根重, 根長 및 根莖에 있어서 Chitofarm 등 天然物質處理區가 無處理區에 대하여 大部分 有意差가 있었으나 天然物質處理區 사이에는 有意性이 없었다. 2. 무 收量에 있어서는 GB$_{10}$ 1%葉面撒布區가 1.7kg으로 check區 보다 75% 增收되었다. 3. 무 作物體 成分分析結果 T-N, $P_2O_5,\;K_2O$,CaO, MgO, Fe, Mn 및 Zn 등의 含量에서 天然物質 無處理區보다 多量이었으므로 品質向上에 效果的이었다.