• Title/Summary/Keyword: Natural components

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Comparison of the Volatile Components of Korean Ginger (Zingiber officinal Roscoe) by Different Extraction Methods (추출방법에 따른 생강의 휘발성 성분 조성 비교)

  • 이재곤;장희진;곽재진;이동욱
    • The Korean Journal of Food And Nutrition
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    • v.13 no.1
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    • pp.66-70
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    • 2000
  • The volatile components of Korea ginger were compared by using different isolation methods, head-space sampling procedure(HSSP), simultaneous distillation extrction(SDE) and soild pahse micro-extractions(SPME). Sixty-one components were identified by GC-MSD in the extracts obtained from each extraction methods. However, the components identified showed a difference in their composition wit the extraction methods. In the extract by HSSP, fifty-five components including a high volatile compound such as acetaldehyde, ethylacetate, 2,3-butandione were detected, and thirty-one components were identified in the extract by SPME. While, the low volatile components such as elemol, zingiberenol and ${\beta}$-eudesmol were detected only in the extract by SDE method. The results suggest that SDE method is the best for the analysis of low volatile components, whereas HSSP is a proper method for the analysis of high volatile components from natural resources.

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Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean

  • Kim, Hye Jeong;Cho, Hyun Suk;Pak, Jun Hun;Kim, Kook Jin;Lee, Dong Hee;Chung, Young-Soo
    • Plant Breeding and Biotechnology
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    • v.5 no.3
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    • pp.237-242
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    • 2017
  • AT-hook proteins of plant have shown to be involved in growth and development through the modification of chromatin architecture to co-regulate transcription of genes. Recently, many genes encoding AT-hook protein have been identified and their involvement in senescence delay is investigated. In this study, soybean transgenic plants overexpressing chromatin architecture-controlling ATPG7 gene was produced by Agrobacterium-mediated transformation and investigated for the positive effect on the important agronomic traits mainly focusing on yield-related components. A total of 27 transgenic soybean plants were produced from about 400 explants. $T_1$ seeds were harvested from all transgenic plants. In the analysis of genomic DNAs from soybean transformants, ATPG7 and Bar fragments were amplified as expected, 975 bp and 408 bp in size, respectively. And also exact gene expression was confirmed by reverse transcriptase-PCR (RT-PCR) from transgenic line #6, #7 and #8. In a field evaluation of yield components of ATPG7 transgenic plants ($T_3$), higher plant height, more of pod number and greater average total seed weight were observed with statistical significance. The results of this study indicate that the introduction of ATPG7 gene in soybean may have the positive effect on yield components.

Effects of the Essential Oil Components from Ligusticum chuanxiong on Proinflammatory Mediators of RAW264.7 Macrophage Cells

  • Lim, Hye-Rim;Shin, Seung-Won
    • Natural Product Sciences
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    • v.16 no.4
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    • pp.259-264
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    • 2010
  • The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural anti-inflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-$1{\beta}$ (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-$\alpha$) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).

Evaluation of High-Resolution Hydrologic Components Based on TOPLATS Land Surface Model (TOPLATS 지표해석모형 기반의 고해상도 수문성분 평가)

  • Lee, Byong-Ju;Choi, Young-Jean
    • Atmosphere
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    • v.22 no.3
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    • pp.357-365
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    • 2012
  • High spatio-temporal resolution hydrologic components can give important information to monitor natural disaster. The objective of this study is to create high spatial-temporal resolution gridded hydrologic components using TOPLATS distributed land surface model and evaluate their accuracy. For this, Andong dam basin is selected as study area and TOPLATS model is constructed to create hourly simulated values in every $1{\times}1km^2$ cell size. The observed inflow at Andong dam and soil moisture at Andong AWS site are collected to directly evaluate the simulated one. RMSEs of monthly simulated flow for calibration (2003~2006) and verification (2007~2009) periods show 36.87 mm and 32.41 mm, respectively. The hourly simulated soil moisture in the cell located Andong observation site for 2009 is well fitted with observed one at -50 cm. From this results, the cell based hydrologic components using TOPLATS distributed land surface model show to reasonably represent the real hydrologic condition in the field. Therefore the model driven hydrologic information can be used to analyze local water balance and monitor natural disaster caused by the severe weather.

Antibacterial Activities of Phenolic Components from Camellia sinensis L. on Pathogenic Microorganisms

  • Shin, Jung-Sook;Chung, Ha-Sook
    • Preventive Nutrition and Food Science
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    • v.12 no.3
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    • pp.135-140
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    • 2007
  • Antibacterial activities of the major phenolic components from Camellia sinensis L. were investigated against several pathogenic microorganisms including Gram-positive strains like Staphylococcus aureus ATCC 29213 and Streptococcus pyogens 308A; and Gram-negative strains like Escherichia coli ATCC 25922, Escherichia coli 078, Pseudomonas aeruginosa 9027, and Enterobacter cloacae 1321E. The MIC values demonstrate that both (-)-epicatechin and (-)-epigallocatechin were more considerably toxic against Staphylococcus aureus ATCC 29213 than the other two catechins like (-)-epicatechingallate and (-)-epigallocatechin-3-gallate. (-)-Epicatechingallate and (-)-epigallocatechin-3-gallate were most inhibitory against Escherichia coli ATCC 25922. As a result, (-)-epicatechin showed predominant antibacterial activities among tea varieties. The contents of major polyphenolic components such as four catechins, theaflavin, and quercetin were different according to fermentation processes. The total contents of four catechins were ranged from 13.81 to 1.33%, with (-)-epigallocatechin-3-gallate being dominant among tea varieties; theaflavin was found the characteristic pigment in fully-fermented black tea.

Comparison of water quality of domestic natural mineral water (국내 먹는샘물의 수질특성비교)

  • Doo, Yong-Kyoon;Kim, Jun-Hwan;Kim, Chang-Su;Chang, Duk
    • Journal of environmental and Sanitary engineering
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    • v.15 no.1
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    • pp.88-94
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    • 2000
  • The purpose of this study was to analyze and compare the contents of minerals and ionic components in domestic commercial natural mineral waters, and to investigate the relationship between water quality and characteristics of suspended and rocks there of its source. All mineral waters tested in this study met the korean mineral water quality guideline, although chemical components varied widely depending upon the geological characteristics and degree of urbanization of the source area or physical water treatment method. The water quality of commercial bottled water was different from that of source water, especially in cationic components, and showed a tendency to become worse with time elapsed. The water quality on the bottle was also different from the measured quality.

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Nucleotide Binding Component of the Respiratory Burst Oxidase of Human Neutrophils

  • Park, Jeen-Woo;Ahn, Soo-Mi
    • BMB Reports
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    • v.28 no.3
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    • pp.275-280
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    • 1995
  • The respiratory burst oxidase of neutrophils is a multicomponent enzyme, domant in resting cells, that catalyzes the reduction of oxygen to $O_{2}^{-}$ at the expense of NADPH. In the resting neutrophil, some of the components of the oxidase, including proteins p47 and p67, are in the cytosol, while the rest are in the plasma membrane. Recent evidence has suggested that at least some of the cytosolic oxidase components exist as a complex. The cytosolic complex with a molecular weight of ~240 kDa was found to bind to blue-agarose and 2',5'-ADP-agarose, which recognize nucleotide requiring enzymes. In order to identify the nucleotide binding component of the cytosolic complex we purified recombinant p47 and p67 fusion proteins using the pGEX system. Pure recombinant p47 was retained completely on 2',5'-ADP-agarose, whereas pure recombinant p67 did not bind to these affinity beads. On the basis of these results, we infer that p47 may contain the nucleotide binding site.

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Alternative Confidence Intervals on the Sum of Variance Components in a Simple Regression Model with Unbalanced Nested Error Structure

  • Park Dong Joon;Lee Soo Jin
    • Communications for Statistical Applications and Methods
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    • v.12 no.1
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    • pp.87-100
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    • 2005
  • In order to construct confidence intervals on the sum of variance components in a simple regression model with unbalanced nested error structure, alternative confidence intervals using Graybill and Wang(1980) and generalized inference concept introduced by Tsui and Weerahandi(1989) are proposed. Computer simulation programmed by SAS/IML is performed to compare the simulated confidence coefficients and average interval lengths of the proposed confidence intervals. A numerical example is provided to demonstrate the confidence intervals and to show consistency between the example and simulation results.

Experimental Study of the Phase Equilibria for $CO_2$ in Liquified Natural Gas Components at 77-219K

  • Yun, Sang-Kook
    • International Journal of Air-Conditioning and Refrigeration
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    • v.11 no.2
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    • pp.61-66
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    • 2003
  • In order to prevent roll-over and a rapid boil-off of LNG in tanks, the phase equilibria of carbon dioxide in liquefied natural gas components as binary mixtures at cryogenic temperatures have been experimentally measured using Fourier transform infrared spectroscopy in conjunction with a specially designed variable pressure/temperature cryostat cell (pathlength 2 mm; pressures up to 30 bar). Solid carbon dioxide has been found to be comparatively soluble in liquid nitrogen (3.25$\times$${10}^{-6}$ mole fraction), liquid methane (1.04$\times$${10}^{-4}$ mole fraction), liquid ethane (3.1$\times$${10}^{-2}$ mole fraction) and liquid propane (6.11$\times$${10}^{-2}$ mole fraction) at their normal boiling temperatures. The solubilities of carbon dioxide in various cryogens, which increased with increasing temperature, are much lower than those obtained by others using gas chromatography. The differences are attributed to infrared spectroscopy selectively measuring dissolved solute in situ whereas gas chromatography measures microscopic particulate solid in addition to dissolved solute.