• Journal of Ginseng Research
• /
• 제47권4호
• /
• pp.543-551
• /
• 2023

Background: Panax ginseng Meyer is a representative Chinese herbal medicine with antioxidant and anti-inflammatory activity. 20(S)-Protopanaxadiol (PPD) has been isolated from ginseng and shown to have promising pharmacological activities. However, effects of PDD on pulmonary fibrosis (PF) have not been reported. We hypothesize that PDD may reverse inflammation-induced PF and be a novel therapeutic strategy. Methods: Adult male C57BL/6 mice were used to establish a model of PF induced by bleomycin (BLM). The pulmonary index was measured, and histological and immunohistochemical examinations were made. Cell cultures of mouse alveolar epithelial cells were analyzed with Western blotting, coimmunoprecipitation, immunofluorescence, immunohistochemistry, siRNA transfection, cellular thermal shift assay and qRT-PCR. Results: The survival rate of PPD-treated mice was higher than that of untreated BLM-challenged mice. Expression of fibrotic hallmarks, including α-SMA, TGF-β1 and collagen I, was reduced by PPD treatment, indicating attenuation of PF. Mice exposed to BLM had higher STING levels in lung tissue, and this was reduced by phosphorylated AMPK after activation by PPD. The role of phosphorylated AMPK in suppressing STING was confirmed in TGF-b1-incubated cells. Both in vivo and in vitro analyses indicated that PPD treatment attenuated BLM-induced PF by modulating the AMPK/STING signaling pathway. Conclusion: PPD ameliorated BLM-induced PF by multi-target regulation. The current study may help develop new therapeutic strategies for preventing PF.

• Smart Structures and Systems
• /
• 제4권1호
• /
• pp.19-34
• /
• 2008

Nowadays developments in the field of laminated composite structures with piezoelectric have attracted significant attention of researchers due to their wide range of applications in engineering such as sensors, actuators, vibration suppression, shape control, noise attenuation and precision positioning. Due to large number of parameters associated with its manufacturing and fabrication, composite structures with piezoelectric display a considerable amount of uncertainty in their material properties. The present work investigates the effect of the uncertainty on the free vibration response of piezoelectric laminated composite plate. The lamina material properties have been modeled as independent random variables for accurate prediction of the system behavior. System equations have been derived using higher order shear deformation theory. A finite element method in conjunction with Monte Carlo simulation is employed to obtain the secondorder statistics of the natural frequencies. Typical results are presented for all edges simply supported piezoelectric laminated composite plates to show the influence of scattering in material properties on the second order statistics of the natural frequencies. The results have been compared with those available in literature.

• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.144.1-144
• /
• 2003

Attenuated viruses can protect their hosts against challenge to their related viruses. Increasing evidence shows that mutations of the tobamoviral 126/183 kDa protein play a major role in the viral attenuation and contribute to the cross protection mechanism. In this study, four mutants of Pepper mild mottle virus (PMMoV) have been constructed by mutagenesis; two mutants, pTPpoly348 and pTPpoly762, were substituted in the middle of replicase gene, and the others, pTPL3D:: $\Delta$6207 and pTPL3D:: $\Delta$6219, were deletion mutants made by deleting some parts of pseudoknot structures of the 3' noncoding region (NCR) of the virus. Progeny viruses generated from the four mutants were infectious on N. benthamiana plants with symptomless or mild mosaic symptom. Replication efficiency and viral product accumulations of four mutants were assessed by Northern and Western blot analyses on BY-2 protoplast cells. Accumulation of CP for the pTPL3D:: $\Delta$6207 and pTPL3D:: $\Delta$6219 were lower than that of other mutants and wild type virus. These data suggest that the 3'-NCR mutations contribute to the viral gene expression in host tissues, while mutants of replicase gene rather govern the symptom expression.

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2009년도 하계학술발표대회 논문집
• /
• pp.284-292
• /
• 2009

The stack-effect in high-rise buildings in winter causes many problems such as difficulties in opening or closing doors, infiltration, energy loss, noise and fire protection. Stack effect is influenced by temperature difference between the interior and exterior of building and the height of building. As an attenuation method for stack effect, the architectural methods are generally used. However, as though architectural methods were fully adopted, the problems are reported as ever in tall building. In this study, a new method to reduce stack effect will be suggested. As an active control method against the stack effect, E/V shaft natural cooling method is suggested. In this paper, the concept of E/V shaft natural cooling system and its reduction performance of stack effect by simulation and field measurement will be reported.

• Plant Biotechnology Reports
• /
• 제4권4호
• /
• pp.243-251
• /
• 2010

Nitric oxide (NO) has been shown to be involved in diverse physiological processes in microbes, animals and plants. In this study, the involvement of NO in the development and possible roles in oxidative stress protection of Chinese cabbage (Brassica rapa subsp. pekinensis cv. Samrack-ulgari) seedlings were investigated. Exogenous application of sodium nitroprusside (SNP) retarded root elongation, while increasing lateral root formation of Chinese cabbage. Plants showed no signs of external stress due to SNP application in true leaves. Cotyledons of 3-week-old Chinese cabbage plants were found to be highly sensitive to SNP application. Treated cotyledons displayed rapid tissue collapse and associated cell death. Although SNP application reduced root growth under normal growth conditions, it also enhanced methyl viologen (MV)-mediated oxidative stress tolerance. Analysis of SNP application to Chinese cabbage leaf disks, revealed SNP-induced tolerance against oxidative stresses by MV and $H_2O_2$, and evidence includes prevention of chlorophyll loss, superoxide anion (${O_2}^-$) accumulation and lipid peroxidation. This report supports a role for nitric oxide in modulating early seedling development, programmed cell death and stress tolerance in Chinese cabbage.

• Clinical and Experimental Reproductive Medicine
• /
• 제42권1호
• /
• pp.1-7
• /
• 2015

Stress coping mechanisms are critical to minimize or overcome damage caused by ever changing environmental conditions. They are designed to promote cell survival. The unfolded protein response (UPR) pathway is mobilized in response to the accumulation of unfolded proteins, ultimately in order to regain endoplasmic reticulum (ER) homeostasis. Various elements of coping responses to ER stress including Perk, Ask1, Bip, Chop, Gadd34, Ire1, Atf4, Atf6, and Xbp1 have been identified and were found to be inducible in oocytes and preimplantation embryos, suggesting that, as a normal part of the cellular adaptive mechanism, these coping responses, including the UPR, play a pivotal role in the development of preimplantation embryos. As such, the UPR-associated molecules and pathways may become useful markers for the potential diagnosis of stress conditions for preimplantation embryos. After implantation, ER stress-induced coping responses become physiologically important for a normal decidual response, placentation, and early organogenesis. Attenuation of ER stress coping responses by tauroursodeoxycholate and salubrinal was effective for prevention of cell death of cultured embryos. Further elucidation of new and relevant ER stress coping responses in periimplantation embryos might contribute to a comprehensive understanding of the regulation of normal development of embryonic development and potentiation of embryonic development in vitro.

• Nuclear Engineering and Technology
• /
• 제51권7호
• /
• pp.1835-1841
• /
• 2019

The mass attenuation coefficient ${\mu}_m$ for eight rock samples having different chemical composition was simulated using the MCNP 5 code in energy range($0.002MeV{\leq}E{\leq}10MeV$). Moreover, the ${\mu}_m$ for the studied rock samples was computed theoretically using XCOM database. The comparison between simulated and computed data for all selected rock samples showed a good agreement with differences varied between 0.01 and 8%. The highest ${\mu}_m$ was found for basalt rocks M2 and M1 and the lowest one is reported for limestone rocks Dike. The simulated values of the ${\mu}_m$ then were used to calculate other important shielding parameters such as the mean free path, effective electron density and effective atomic number. The exposure buildup factor EBF was also computed for the selected rocks with the contribution of G-P fitting parameters and the highest EBF attended by the basalt sample Sill and varied between 1.022 and 744 in the energy range between ($0.015MeV{\leq}E{\leq}15MeV$) but the lowest EBF achieved by basalt sample M2 and varied between 1.017 and 491 in the same energy range.

• Biomolecules & Therapeutics
• /
• 제27권2호
• /
• pp.210-215
• /
• 2019

Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.

• 방송공학회논문지
• /
• 제21권6호
• /
• pp.889-898
• /
• 2016

가상 저음 시스템은 기본 주파수 성분의 배음을 생성하여 스피커의 저역 재생 대역을 확장하는 방법으로 소형 스피커에 널리 사용된다. 가상 저음 시스템의 주관적인 성능은 배음의 가중치 부여 방법과 관련이 높기 때문에, 기존 연구에서는 지수 감쇠 가중치 부여 방법과 음색 매칭 방법 등 다양한 가중치 부여 방법이 제안되었다. 그러나 생성한 배음과 기존 신호간의 위상을 맞추지 않을 경우 정확한 가중치 부여가 불가능하다. 본 논문에서는 기존 가중치 부여 방법의 한계점을 분석하고 이를 개선한 가중치 부여 방법을 제안하였다. 제안한 방법은 생성한 배음의 위상을 기존신호의 위상과 일치시키고, 기본 주파수에 따라 배음 가중치를 가변적으로 부여하는 방법이다. 기존 가상 저음 시스템과 객관 및 주관 비교 평가를 수행한 결과, 위상 일치 방법은 자연스럽고 효과적인 저역강화에 필수적임을 확인하였으며, 제안한 배음 가중치 부여 방법은 제한된 상황에서 기존 가중치 부여 방법보다 효과적임을 확인하였다.

• 한국해양공학회지
• /
• 제32권6호
• /
• pp.485-491
• /
• 2018

Because of the increase in coastal erosion problems, many studies have been conducted to prevent coastline retreat by developing low-cost, highly effective countermeasures. We developed the artificial coral reefs (ACRs) method as part of this research trend. To verify its coastal protection performance, we carried out performance tests on its wave attenuation and beach sand capture ability, which are the key barometers for this newly developed technology. In this study, three different types of methods, including natural beach, TTP, and ACRs, were used to determine the coastal protection efficiency under both ordinary and storm wave conditions. Based on the results of this study, ACRs were found to have the best wave attenuation performance and captured more than 20% of the total erosion area. This means the ACR method can be applied as a reliable countermeasure to protect a coastal zone.