• 생명과학회지
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• 제30권11호
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• pp.1007-1011
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• 2020

본 연구는 우리나라 고유의 재래흑염소 집단인 당진, 장수, 통영 및 경상대 계통과 교잡종 염소 계통 또는 해외품종의 개체 식별을 위한 유전적 다양성과 관계 조사 및 검증을 위해 수행하였다. 각 염소 집단에 존재하는 Monomorphic SNP를 수집한 이후 공통적으로 존재하는 SNP 133개를 선발하여 분석에 이용하였다. Monomorphic SNP 133개를 통한 재래흑염소와 교잡종 염소의 유전적 구조 차이를 나타냈으며, 주성분 분석 결과 재래흑염소와 교잡종 염소가 명확히 구분되는 것으로 나타났다. 또한, 참조집단 이외의 70두(Native Korean goat = 24, Cross breed = 46)로 구성된 검증집단을 분석한 결과 국내 재래흑염소 계통의 참조집단과 동일한 유전적 구조를 나타냈으며, 교잡종 염소의 경우 참조집단의 일부 유전적 구성을 공유하는 것으로 나타났다. 국내 재래흑염소의 경우는 하나의 군집을 형성한 반면 해외 품종 및 교잡종 계통의 경우 재래흑염소 계통에 비해 넓게 퍼져 군집을 형성하는 것으로 나타났다. 따라서, 본 연구 결과는 국내 재래흑염소 유전자원 집단을 보존을 위한 기초자료로 활용하고 추후 유전적 다양성을 고려한 염소의 개량을 위한 기초자료로 유용하게 활용 할 수 있을 것으로 판단된다.

• 생명과학회지
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• 제30권10호
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• pp.912-918
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• 2020

본 연구는 재래흑염소와 교잡종 염소 총 304두를 대상으로 Microsatellite (MS) marker의 대립유전자형 분석을 통해 염소의 개체식별과 친자확인을 목적으로 실시하였다. 각 MS marker 별 대립유전자형의 다형성을 토대로 11종의 MS marker를 선발하였다. 선발된 MS marker를 사용할 경우 동일한 유전자형을 가진 개체가 출현할 확률이 무작위, 반형매 교배집단에서 각각 5.58×10^-10, 1.15×10^-7으로 분석되었다. 또한 친자감정 확률은 부모의 정보가 있을 경우 0.999996, 부모의 정보가 없을 경우 0.999833으로 분석되어 국내에서 사육하고 있는 염소들의 개체식별 및 친자확인이 가능할 것으로 사료된다. 또한 국내 재래흑염소 4 계통과 교잡종 염소들 간의 혈연관계 분석을 통해 국내 재래흑염소의 유전적 특성을 확인하였다. 본 연구의 결과는 염소의 개량 기반 구축에 필요한 개체관리와 친자감별 및 향후 염소고기의 생산 이력 구축에 유용하게 활용 할 수 있을 것으로 판단된다.

• 한국동물위생학회지
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• 제18권1호
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• pp.1-21
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• 1995

To elucidate pathogenesis of bovine leukemia virus(BLV) in Korean native goats, the goats experimentally infected with BLV were studied especially for the aspects of infectivity and hematological changes. The experimental goats were examined for 27 months by agar-gel immunodiffusion(AGID) test and syncytium formation assay. During this period, changes of total leucocyte, absolute Iymphocyte and atypical Iymphocyte were examined, and the distribution of surface immunoglobulin ( sIg ) -bearing cells and rosette forming cell (RFC) in the peripheral Iymphocyte were also investigated. By indirect immunofluorescence (IFA) and complement dependent antibody cytotoxicity (CDAC) assay using monoclonal antibody(Mab) against bovine leukosis tumor-associated anti-gen(BL-TAA), changes of BL-TAA positive Iymphocyte in peripheral blood were measured. The results obtained through the experiment were summarized as follows. 1. Antibody titers were measured by AGID using gP51 and P24 antigens. The animals were serologically converted at 2 months post-inoculation(pi) in gP51 antigen, whereas sero-converted at 4 months pi in P24 antigen. In comparison with antibody titers for gP51, P24 antigen showed lower titers throughout the trial period. 2. The peripheral lymphocytes from all of the infected goats, as co-cultivated with F8l cells manifested syncytial formation at 4 months pi. 3. On counting total leucocyte, Iymphocyte and atypical Iymphocyte, two out of four infected goats showed normal distribution, while No 2 of the remaining two revealed temporal and No 3, Persistant increasing number of the cells. 4. The optimal condition of rosette formation of the peripheral Iymphocyte of normal Korean native goats was shown in the sheep erythrocyte treated with 0.1M AET for 30 nun at $37^{\circ}C$. When the Iymphocytes were treated in nylon wool column, the number of sIg-bear-ing cell were increased in the nylon wool adherent cells, but RFC was increased in the non-adherent cells. Of the infected goats, No 2 and No 3 showed significantly increasing number of sIg-bearing cells at 18 months pi. 5. The Iymphocytes of No 2 and No 3 goats reacted positively in IFA using Mab against BL-TAA at 12 months pi and 18 months pi, respectively. In CDAC test, all of four infected goats revealed positive reaction at 24 months pi. The higher positive rates were observed in No 2 and No 3 as compared with the remainders.

• 한국임상수의학회지
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• 제23권1호
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• pp.14-17
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• 2006

본 연구에서는 충남 공주근교에서 사육중인 한국재래산양에서 자연 발생한 전염성 농포성 피부염에 대하여 오존의 치료효과를 조사하였다. 전염성 농포성 피부염은 한국재래산양 150두 중 130두에서 발생하였다. 16두(2개월-2년)를 선발하여 대조군(5두), ozonated oil군(5두) 및 ozonated water군(6두)으로 구분하였다. 대조군에서는 실험기간 중 아무런 치료를 하지 않았으며, ozonated oil군은 식물성유에 오존가스(200ppm)로 3일간 bubbling한 ozonated oil을 환부에 1일 1회 2주간 도포하였고, ozonated water군은 수도물에 오존가스(200ppm)로 1시간 동안 bubbling한 ozonated water(0.1ppm)를 2주간 자유로이 음수토록 하였다. 그 결과, 치료율은 ozonated oil군이 80%(4/5두), ozonated water군이 66.7%(4/6두)로, 대조군(20%, 1/5두)보다 양호하였다. 이 결과를 근거로 전염성 농포성 피부염에 이환된 한국재래 산양 121두를 대상으로 ozonated oil을 1일 1회, 30일간 환부에 도포하였다. 그 결과, 환축의 모든 병변부는 완치되었다. 이상의 결과를 종합해 볼 때, 오존요법은 산양의 전염선 농포성 피부염의 치료에 유효한 것으로 판단되었다.

• 한국동물위생학회지
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• 제17권3호
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• pp.190-197
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• 1994

In order to monitor the parasites, 303 fecal samples were taken from Korean native goats (n=279) and sheep(n=24) in Chonbuk area. The identification of the parasites were determimed by the fecal examination using the floatation or sedimentation methods and microscopical examination, respectively. The obtained results were summarized as follows ; 1. The detection rate of the parasites from Korean native goats and sheep was 98.2% and 83.3%, respectively. 2. In Korean native goat, mixed infection rate was single 7.5%(21 heads), double 47.3%(132 heads), triple 40.8%(114 heads), quadraple 5.0%(14 heads) and quintuple 2.5%(7 heads). 3. In sheep, mixed infection rate was single 12.5%(3 heads), double 45.8%(13 heads) and triple 16.6%(4 heads). 4. The parasites isolated were identified as Eimeria spp. from 283 heads, Oesophagostomum spp. from 96 heads, Ostertagia spp. from 97 heads, Strongylodes papillus from 97 heads, Heamonchs spp. from 73 heads, Trichostrongylus spp. from 42 heads, Moniezia expenza from 26 heads, Cooperia spp. from 16 heads, Nematodirus spp. from 9 heads, Bonostomum spp. from 9 heads, Paraphistomum spp. from 7 heads, and Fasciola hepatica and Capillaria spp. In 2 heads, in order.

• 대한수의학회지
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• 제39권5호
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• pp.908-920
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• 1999

We investigated the cytogenetic characteristics of Korean native goat(Capra hircus). Chromosome slides were prepared from peripheral blood cell cultures. GTG, GBG, RBG and CBG-banding techniques were employed on those slides. The high resolution karyotype of Korean native goat could be made with the incorporation of BrdU. Korean native goat has 60 chromosomes composed of 58 autosomes and XY or XX sex chromosomes. All of autosomes of Korean native goat were acrocentric chromosomes. X chromosome was submetacentric and Y chromosome was metacentric. The GTG, GBG and RBG-band patterns of Korean native goat were similar to those of other goats. CBG-band regions were distinct at the proximal portion of the long arms of all autosomes in Korean native goats. According to our investigation, there was no significant difference in chromosomal band patterns between Korean native goat and other goats. It might be necessary to use molecular genetic markers for clarifying the genetical characteristics of Korean native goat whose biological characteristics are not clearly defined.

• 한국초지조사료학회지
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• 제14권1호
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• pp.27-33
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• 1994

This study was conducted to determine the nutritional value of native oriental white oak(Quercus aliena Blume) browse by Korean native goats. Oak browse were mixed with grass hay(orchardgrass 60% and red clover 40%), varing in oak browse content. Diets included 30% oak browse and 70% grass hay, 60% oak browse and 40 % grass hay, and a 100% grass hay(contro1). Oak browse diets were higher in NDF, ADF, lignin, and tannin contents, but lower in CP content than the control diet with increased oak browse levels. Gross energy levels showed no difference between the control and oak browse diets. Dry matter intake was the lowest on diet containing oak browse 60%. Digestibility for DM, cellular constituents, NDF, and ADF showed lower for oak browse diets compared to control diet(P<0.05). Oak browse diets furnished significantly lower dietary N than &d the control diet. Fecal N losses generally increased with higher levels of oak browse levels(P<0.05). Apparently digested N and retained N were lower in oak browse diets than in control diet. The higher retained N was obtained in control diet than in oak browse diets. DE and ME declined linearly as oak browse levels increased when compared to control diet(P

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.573-579
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• 1998

The effects of supplementation with mixed fodder or concentrate were investigated on the performance of a) growth of male lambs and kids grazing extensively under oil palm trees and b) reproduction of ewes and goats stall-fed with native herbage. Supplemented kids and lambs had higher average daily weight gain than controls but the effect was only significant for those which received concentrate (p < 0.05). Final body weight was only significantly different from controls for lambs supplemented with concentrate (p < 0.05). The ages at puberty, first mating, first conception and first kidding of supplemented goats were about 110 days earlier than those for controls (p < 0.05 or better). Supplemented goats had first mating and conceived at lower body weights (p < 0.01) than those in control groups. Different feeding regimes had no effects (p > 0.05) on the reproductive performance of ewes apart from highest body weight of first lambing in animals supplemented with concentrate (p < 0.05). Native herbage available under oil palm trees of 5 years old was sufficient for growth and reproduction of sheep and goats. Concentrate was better than mixed fodder as supplement for enhancing the reproductive performance of goats but both appeared to have limited effects on the performance of reproduction in ewes and growth in male kids.

• 대한수의학회지
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• 제58권4호
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• pp.171-175
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• 2018

The aim of this study was to investigate morphological development of filiform papillae (FP) in Korean native goats by using scanning electron microscopy. Tongues were removed from goat fetuses (days 60, 90, and 120), neonates, and juveniles (days 30, 60, 90, 120, 150, and 180 after birth). During the prenatal period, primordia of FP appeared at fetal day 60 and were observed to be developed at day 90. At fetal day 120, the FP were observed like flower leaves of a double flower bud. In neonates, FP were shaped like an obliquely sectioned cylinder with secondary papillae irregularly arranged in a saw blade-like manner. In 60-day-old juvenile goats, the FP were densely distributed at the inner base of 1/3-1/2 degrees. In 90-, 120-, and 150-day-old goats, FP were compacted at the inner base of 1/2-2/3, 3/4, and 4/5 degrees, respectively. In 180-day-old goats, FP were found to be completely compacted on the inner surface with complete morphogenesis. Microridges, microplicae, and micropits were well-developed on the epithelial surface of lingual papillae from embryonic day 120 to juvenile day 180. These results indicate that FP of goats have different shapes and sizes during development both before and after birth.

• 농업과학연구
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• 제48권4호
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• pp.693-702
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• 2021

The aim of this study was to analyze the genetic diversity and distance of the Korean native black goat line. Thus far, this Korean native black goat line has not been studied intensively, especially in genetic diversity and relationship studies in comparison with other breeds. In total, eleven microsatellite (MS) markers were used to evaluate alleles from 391 Korean native black goats and foreign hybrid animals. The genetic diversity index was evaluated based on the allele distributions. Four Korean native black goat lines showed expected ranges of observed heterozygosity, expected heterozygosity, and polymorphism information content (PIC) values for use in genetic diversity research (0.509 - 0.643, 0.434 - 0.623 and 0.356 - 0.567). Lines from the Korean native black goat and foreign hybrid were clearly separated according to principal coordinates analysis (PCoA), phylogenetic tree and tended to be clustered in each Korean native black goat line. Thus, this study can be used for analyzing the genetic relationships between Korean native black goats and foreign breeds for line preservation and for fundamental information to determine breed improvement strategies.