• Journal of Life Science
• /
• v.30 no.11
• /
• pp.1007-1011
• /
• 2020

This study was conducted to analyze the genetic diversity and relationships that discriminate between Korean native black goat populations (Dangjin, Jangsu, Tongyoung, and Gyeongsang National University strains) and crossbred goats. Monomorphic single nucleotide polymorphisms (SNPs) in each strain were collected, and 133 common SNPs were selected for analysis. These 133 monomorphic SNPs showed differences in the genetic structure of the Korean native black goat and crossbred goats, and results from the principal component analysis (PCA) showed that the two can be clearly separated. Furthermore, analysis of the validation population comprising 70 individuals (Korean native black goats, n = 24; crossbred goats, n = 46) with the reference population showed that Korean native black goat strains and the reference population have the same genetic structure, and the crossbred goats shared only part of the genetic structure with the reference population. The result of the PCA analysis showed that the Korean native black goat strains form one population, whereas the foreign strains form another population which is more widely dispersed than the Korean native black goat strains. Thus, the results from this study can be used as baseline data for the conservation of genetic resources of Korean native black goat communities through utilization of monomorphic SNPs and for the introduction of exotic species for further improvement in genetic diversity. This study can also help reduce unnecessary inbreeding and gene flow between native strains.

• Journal of Life Science
• /
• v.30 no.10
• /
• pp.912-918
• /
• 2020

The Korean native black goat (Capra hircus coreanae) is the goat species to be officially registered in Korea under the Food and Agriculture Organization. The object of this study is to establish a set of microsatellite (MS) markers for the individual identification and parentage verification of goats. In this study, we analyzed alleles of MS markers in crosses between Korean native black goats and crossbred goats (n=304 animals), and, based on the diversity of alleles for each marker, we selected 11 MS markers for individual identification and parentage verification. Using these 11 MS markers, the probabilities of different individuals with the same genotype being found within random and half-sib mating populations were 5.58×10^-10 and 1.15×10^-7, respectively. The parentage verification accuracy was 0.999996 when information about the parents was available and 0.999833 with no information. Thus, even given the total rearing population of 576,150 animals in South Korea, we concluded that these markers could be used for the individual identification and parentage verification of goats. Moreover, by analyzing the genetic relationships between the four lines of Korean native black goats and the crossbred goats, we verified the genetic characteristics of Korean native black goats, confirming their conservation value as a unique genetic resource.

• Korean Journal of Veterinary Service
• /
• v.18 no.1
• /
• pp.1-21
• /
• 1995

To elucidate pathogenesis of bovine leukemia virus(BLV) in Korean native goats, the goats experimentally infected with BLV were studied especially for the aspects of infectivity and hematological changes. The experimental goats were examined for 27 months by agar-gel immunodiffusion(AGID) test and syncytium formation assay. During this period, changes of total leucocyte, absolute Iymphocyte and atypical Iymphocyte were examined, and the distribution of surface immunoglobulin ( sIg ) -bearing cells and rosette forming cell (RFC) in the peripheral Iymphocyte were also investigated. By indirect immunofluorescence (IFA) and complement dependent antibody cytotoxicity (CDAC) assay using monoclonal antibody(Mab) against bovine leukosis tumor-associated anti-gen(BL-TAA), changes of BL-TAA positive Iymphocyte in peripheral blood were measured. The results obtained through the experiment were summarized as follows. 1. Antibody titers were measured by AGID using gP51 and P24 antigens. The animals were serologically converted at 2 months post-inoculation(pi) in gP51 antigen, whereas sero-converted at 4 months pi in P24 antigen. In comparison with antibody titers for gP51, P24 antigen showed lower titers throughout the trial period. 2. The peripheral lymphocytes from all of the infected goats, as co-cultivated with F8l cells manifested syncytial formation at 4 months pi. 3. On counting total leucocyte, Iymphocyte and atypical Iymphocyte, two out of four infected goats showed normal distribution, while No 2 of the remaining two revealed temporal and No 3, Persistant increasing number of the cells. 4. The optimal condition of rosette formation of the peripheral Iymphocyte of normal Korean native goats was shown in the sheep erythrocyte treated with 0.1M AET for 30 nun at $37^{\circ}C$. When the Iymphocytes were treated in nylon wool column, the number of sIg-bear-ing cell were increased in the nylon wool adherent cells, but RFC was increased in the non-adherent cells. Of the infected goats, No 2 and No 3 showed significantly increasing number of sIg-bearing cells at 18 months pi. 5. The Iymphocytes of No 2 and No 3 goats reacted positively in IFA using Mab against BL-TAA at 12 months pi and 18 months pi, respectively. In CDAC test, all of four infected goats revealed positive reaction at 24 months pi. The higher positive rates were observed in No 2 and No 3 as compared with the remainders.

• Journal of Veterinary Clinics
• /
• v.23 no.1
• /
• pp.14-17
• /
• 2006

We investigated possible therapeutic approaches to treat contagious naturally occurring ecthyma in Korean native goats in the Gongju area of Korea. Contagious ecthyma was found in 130 of 150 Korean native goats studied. Sixteen goats, ranged from 2 months to 2.5 years old, were selected for further experiments. The goats were divided into a control group, an ozonated oil group, and an ozonated water group consisting of 5, 5, and 6 goats, respectively. The control animals were not treated during the experimental period. The ozonated oil group was treated with ozonated oil, made by bubbling 200 ppm ozone gas into the oil, dally for 2 weeks. The remaining group of animals were given ozonated drinking water (0.1 ppm) ad libitum for 2 weeks. The ozonated oil treated group (4/5, 80%) had the highest therapeutic rate. The ozonated water group (4/6, 66.7%) had the second highest therapeutic rate, followed by the control group (1/5, 20%). Based on these results, a secondary experiment was performed that included a total of 121 Korean native goats with contagious ecthyma. Ozonated oil was applied daily for 30 days. All of the lesions on the goats with contagious ecthyma were completely cured by application of the ozonated oil. We concluded that ozone therapy was an effective treatment for contagious ecthyma in goats

• Korean Journal of Veterinary Service
• /
• v.17 no.3
• /
• pp.190-197
• /
• 1994

In order to monitor the parasites, 303 fecal samples were taken from Korean native goats (n=279) and sheep(n=24) in Chonbuk area. The identification of the parasites were determimed by the fecal examination using the floatation or sedimentation methods and microscopical examination, respectively. The obtained results were summarized as follows ; 1. The detection rate of the parasites from Korean native goats and sheep was 98.2% and 83.3%, respectively. 2. In Korean native goat, mixed infection rate was single 7.5%(21 heads), double 47.3%(132 heads), triple 40.8%(114 heads), quadraple 5.0%(14 heads) and quintuple 2.5%(7 heads). 3. In sheep, mixed infection rate was single 12.5%(3 heads), double 45.8%(13 heads) and triple 16.6%(4 heads). 4. The parasites isolated were identified as Eimeria spp. from 283 heads, Oesophagostomum spp. from 96 heads, Ostertagia spp. from 97 heads, Strongylodes papillus from 97 heads, Heamonchs spp. from 73 heads, Trichostrongylus spp. from 42 heads, Moniezia expenza from 26 heads, Cooperia spp. from 16 heads, Nematodirus spp. from 9 heads, Bonostomum spp. from 9 heads, Paraphistomum spp. from 7 heads, and Fasciola hepatica and Capillaria spp. In 2 heads, in order.

• Korean Journal of Veterinary Research
• /
• v.39 no.5
• /
• pp.908-920
• /
• 1999

We investigated the cytogenetic characteristics of Korean native goat(Capra hircus). Chromosome slides were prepared from peripheral blood cell cultures. GTG, GBG, RBG and CBG-banding techniques were employed on those slides. The high resolution karyotype of Korean native goat could be made with the incorporation of BrdU. Korean native goat has 60 chromosomes composed of 58 autosomes and XY or XX sex chromosomes. All of autosomes of Korean native goat were acrocentric chromosomes. X chromosome was submetacentric and Y chromosome was metacentric. The GTG, GBG and RBG-band patterns of Korean native goat were similar to those of other goats. CBG-band regions were distinct at the proximal portion of the long arms of all autosomes in Korean native goats. According to our investigation, there was no significant difference in chromosomal band patterns between Korean native goat and other goats. It might be necessary to use molecular genetic markers for clarifying the genetical characteristics of Korean native goat whose biological characteristics are not clearly defined.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.14 no.1
• /
• pp.27-33
• /
• 1994

This study was conducted to determine the nutritional value of native oriental white oak(Quercus aliena Blume) browse by Korean native goats. Oak browse were mixed with grass hay(orchardgrass 60% and red clover 40%), varing in oak browse content. Diets included 30% oak browse and 70% grass hay, 60% oak browse and 40 % grass hay, and a 100% grass hay(contro1). Oak browse diets were higher in NDF, ADF, lignin, and tannin contents, but lower in CP content than the control diet with increased oak browse levels. Gross energy levels showed no difference between the control and oak browse diets. Dry matter intake was the lowest on diet containing oak browse 60%. Digestibility for DM, cellular constituents, NDF, and ADF showed lower for oak browse diets compared to control diet(P<0.05). Oak browse diets furnished significantly lower dietary N than &d the control diet. Fecal N losses generally increased with higher levels of oak browse levels(P<0.05). Apparently digested N and retained N were lower in oak browse diets than in control diet. The higher retained N was obtained in control diet than in oak browse diets. DE and ME declined linearly as oak browse levels increased when compared to control diet(P

• Asian-Australasian Journal of Animal Sciences
• /
• v.11 no.5
• /
• pp.573-579
• /
• 1998

The effects of supplementation with mixed fodder or concentrate were investigated on the performance of a) growth of male lambs and kids grazing extensively under oil palm trees and b) reproduction of ewes and goats stall-fed with native herbage. Supplemented kids and lambs had higher average daily weight gain than controls but the effect was only significant for those which received concentrate (p < 0.05). Final body weight was only significantly different from controls for lambs supplemented with concentrate (p < 0.05). The ages at puberty, first mating, first conception and first kidding of supplemented goats were about 110 days earlier than those for controls (p < 0.05 or better). Supplemented goats had first mating and conceived at lower body weights (p < 0.01) than those in control groups. Different feeding regimes had no effects (p > 0.05) on the reproductive performance of ewes apart from highest body weight of first lambing in animals supplemented with concentrate (p < 0.05). Native herbage available under oil palm trees of 5 years old was sufficient for growth and reproduction of sheep and goats. Concentrate was better than mixed fodder as supplement for enhancing the reproductive performance of goats but both appeared to have limited effects on the performance of reproduction in ewes and growth in male kids.

• Korean Journal of Veterinary Research
• /
• v.58 no.4
• /
• pp.171-175
• /
• 2018

The aim of this study was to investigate morphological development of filiform papillae (FP) in Korean native goats by using scanning electron microscopy. Tongues were removed from goat fetuses (days 60, 90, and 120), neonates, and juveniles (days 30, 60, 90, 120, 150, and 180 after birth). During the prenatal period, primordia of FP appeared at fetal day 60 and were observed to be developed at day 90. At fetal day 120, the FP were observed like flower leaves of a double flower bud. In neonates, FP were shaped like an obliquely sectioned cylinder with secondary papillae irregularly arranged in a saw blade-like manner. In 60-day-old juvenile goats, the FP were densely distributed at the inner base of 1/3-1/2 degrees. In 90-, 120-, and 150-day-old goats, FP were compacted at the inner base of 1/2-2/3, 3/4, and 4/5 degrees, respectively. In 180-day-old goats, FP were found to be completely compacted on the inner surface with complete morphogenesis. Microridges, microplicae, and micropits were well-developed on the epithelial surface of lingual papillae from embryonic day 120 to juvenile day 180. These results indicate that FP of goats have different shapes and sizes during development both before and after birth.

• Korean Journal of Agricultural Science
• /
• v.48 no.4
• /
• pp.693-702
• /
• 2021

The aim of this study was to analyze the genetic diversity and distance of the Korean native black goat line. Thus far, this Korean native black goat line has not been studied intensively, especially in genetic diversity and relationship studies in comparison with other breeds. In total, eleven microsatellite (MS) markers were used to evaluate alleles from 391 Korean native black goats and foreign hybrid animals. The genetic diversity index was evaluated based on the allele distributions. Four Korean native black goat lines showed expected ranges of observed heterozygosity, expected heterozygosity, and polymorphism information content (PIC) values for use in genetic diversity research (0.509 - 0.643, 0.434 - 0.623 and 0.356 - 0.567). Lines from the Korean native black goat and foreign hybrid were clearly separated according to principal coordinates analysis (PCoA), phylogenetic tree and tended to be clustered in each Korean native black goat line. Thus, this study can be used for analyzing the genetic relationships between Korean native black goats and foreign breeds for line preservation and for fundamental information to determine breed improvement strategies.