• Title/Summary/Keyword: National Research and Development Information

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cDNA Cloning and Expression of a Cytochrome P450 1A (CYP1A) from the Pale Chub, Zacco platypus

  • Jeon, Hyoung-Joo;Park, Young-Chul;Lee, Wan-Ok;Lee, Jong-Ha;Kim, Jin-Hyoung
    • Korean Journal of Ecology and Environment
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    • v.44 no.4
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    • pp.364-372
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    • 2011
  • The pale chub (Zacco platypus) is generally found in Asian countries, such as Korea, Japan, and China. Nevertheless, very little information exists about the genes involved in the metabolism of xenobiotics in this species. This species is useful in monitoring the environmental impact on various pollutants in freshwater as a sentinel fish species. We cloned the full-length cDNA sequence of xenobiotic metabolizing cytochrome P450 1A (CYP1A) gene from Z. platypus and characterized it. Tissue distribution and timedependent induction of CYP1A were studied by real-time RT-PCR. Induction pattern of CYP1A was studied by exposing the fish to an arylhydrocarbon receptor agonist, ${\beta}$-naphthoflavone (BNF). The liver showed the highest level of expression in basal state as well as BNF- treated fish. However, appreciable levels of expression were also recorded in Gill and kidney and the least level of expression was observed in the eye. The results of the time-course study revealed an induction in the liver, brain, and gills after 6 h and 12 h in most of the tissues. This study provides an insight into the xenobiotics metabolizing system of Z. platypus and offers baseline information for further research related to biomarker, stress, and adaptive response of this ecologically important fish species in the freshwater environment.

A Study on the National Spatial Data Infrastructure of U.S.A

  • Koh, June-Hwan
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.25 no.6_1
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    • pp.485-497
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    • 2007
  • By the rapid development of Information Communication Technology (ICT) and Geo-spatial Technology (GT) and the increased usage of spatial data for planning and infrastructure management, the National Geographic Information System (NGIS) for more efficient and effective utilization of spatial information has been developed by the central government in Korea since 1995. NGIS is the base of Spatial Data Infrastructure (SDI). SDI is developed as one of National Information Infrastructures (NII). Among the hierarchy of SDI, National Spatial Data Infrastructure (NSDI) has very important role in the success of SDI development. Many research articles show that the USA's NSDI initiatives, development strategy have been strongly influenced all over the world. In these viewpoints, to propose the future directions of Korean NGIS, the development of NSDI strategy of USA is reviewed by literature through published book and internet resources. The conclusions of this study are as follow: 1) top-down and bottom-up approach are needed for integrated data sharing and standardization. 2) the creative and evolutionary vision and strategy has to be suggested. 3) the training program and lecture material has to be developed and diffused to the users and providers of spatial data. 4) governance system has to be built for NSDI evaluation. 5) the formation of geo-spatial forum to discuss the spatial-related problems and make research agenda, etc.

Transcriptome analysis, microsatellite marker information, and orthologous analysis of Capsicum annuum varieties

  • Ahn, Yul-Kyun;Karna, Sandeep;Kim, Jeong-Ho;Lee, Hye-Eun;Kim, Jin-Hee;Kim, Do-Sun
    • Journal of Plant Biotechnology
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    • v.43 no.3
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    • pp.311-316
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    • 2016
  • The efficacy of plant breeding has been enhanced by application of molecular markers in population screening and selection. Pepper (Capsicum annuum L.) is a major staple crop that is economically important with worldwide distribution. It is valued for its spicy taste and medicinal effect. The aim of this study was to discover single nucleotide polymorphisms (SNPs), microsatellite markers information, and percentage sharing through orthologous analysis of pepper-specific pungency-related genes. Here, we report the results of transcriptome analysis and microsatellite markers for four pepper varieties that possess a pungency-related gene. Orthologous analyses was performed to identify species-specific pungency-related genes in pepper, Arabidopsis thaliana L., potato (Solanum tuberosum L.), and tomato (Solanum lycopersicum L.). Advancements in next-generation sequencing technologies enabled us to quickly and cost-effectively assemble and characterize genes to select molecular markers in various organisms, including pepper. We identified a total of 9762, 7302, 8596, and 6886 SNPs for the four pepper cultivars Blackcluster, Mandarine, Saengryeg 211, and Saengryeg 213, respectively. We used 454 GS-FLX pyrosequencing to identify microsatellite markers and tri-nucleotide repeats (54.4%), the most common repeats, followed by di-, hexa-, tetra-, and penta-nucleotide repeats. A total of 5156 (15.9%) pepper-specific pungency-related genes were discovered as a result of orthologous analysis.

Optimization of Conditions for Two-Dimensional Electrophoresis of the Flounder (Paralichthys olivaceus) Serum (넙치(Paralichthys olivaceus) 혈청단백질의 이차원전기영동분석 조건확립)

  • Han, Yoon Hee;Nam, Bo-Hye;Kim, Young-Ok;Kim, Woo Jin;Kong, Hee Jeong;Lee, Sang Jun;Choi, Tae-Jin
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.1
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    • pp.68-72
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    • 2007
  • Flounder is one of the major aquacultured fish and of an economically important item in Korean fisheries. Recently, there are trends of research worldwide that aim to analyze and characterize a whole genome or a whole proteome of interesting species. The data are utilized for the understanding and development of preventive and curative technologies for the serious diseases. However, there are very limited information of proteome for marine organisms, we optimized first the conditions for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with serum form a marine fish, flounder (Paralichthys olivaceus). A pre-treatment of serum and an optimization of protein concentration analyzed were surveyed for enhancing the separation for proteins. A statistical analysis was performed on the overall 1,820 protein spots to overcome the variability among individual fishes.

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Analysis of the Librarian Certification System in Korea and Measures for Improvement

  • Yoon, Hee-Yoon;Kim, Sin-Young
    • International Journal of Knowledge Content Development & Technology
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    • v.11 no.3
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    • pp.45-62
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    • 2021
  • Half a century has passed since the academic field of library and information science, which was born at the end of the 19th Century in the Western world, was introduced to Korea. Despite years of quantitative growth and qualitative maturation, the field has been facing the inconvenient truths of discrepancies in education levels arising from varying education providers, and weakened public confidence in librarian certification. Based on analysis of librarian training and education programs and the certification system, this research seeks to point out the limitations of the national librarian certification system, which is losing public confidence, and present a model for improvement. Library and information science education has expanded from universities to the academy of librarian education and lifelong education centers, and the librarian certification system has emerged as a target of reform. This research presents measures to improve the librarian certification system to government bodies and the Committee on Library and Information Policy with the purpose of providing grounds for policy enforcement.

Analysis of Patent Portfolio for Intellectual Property R&D Strategy of Beauty Instruments (미용기기 분야 IP R&D 전략을 위한 특허 포트폴리오 분석)

  • Koh, Chang-In;Lee, Young-Seok
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.10 no.1
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    • pp.117-124
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    • 2017
  • In this paper, we analyzed the technology competitiveness of main competitors based on the patent trend analysis for beauty instruments fields, and drew up the patent reserve strategy and patent portfolio for project related technologies according to technical trends analysis. Using the analysis result of patent portfolio to establish the strategic IP based research and development plans, the patent wall and gap areas are suggested, And, based on the basic information to set up the direction of research and development, we intend to provide objective patent information for the feasibility of conducting research and development tasks and for the avoidance of duplication research.

Development of the Slurry Type Diet for the Growing Leptocephalus, Eel Larvae (Anguilla japonica) (뱀장어 자어용 액상사료 개발)

  • Kim, Shin-Kwon;Lee, Bae-Ik;Kim, Dae-Jung;Lee, Nam-Sil
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.6
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    • pp.1209-1216
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    • 2014
  • This feeding experiment was conducted to investigate the effects of slurry type diet on growth performance and survival rate of growing leptocephalus, eel larva. We need to find new materials of diets for rearing eel larvae. Test diets were formulated with the eggs of the shark, fish soluble concentrate, soybean peptide and fish protein hydrolysate. Fish (average length 6 mm) were fed 3 slurry type diet(A, B and C) based on shark egg for 5 times per day. During feeding experiment, survival rates were significantly different among 3 slurry type diets. Total protein, lipid, moisture, ash and free amino acids contents were analyzed for slurry type diets. Leptocephalus fed the C slurry type diet was grown up to $38.0{\pm}9mm$ at 150 days. But all leptocephalus fed B slurry type diet were died at 100 days, reaching $16.4{\pm}8mm$. This results suggest that basic information for diet development of eel leptocephalus.

DESIGN OF STANDARD GRIDDED METADATA FOR INTEGRATED MANAGEMENT OF SATELLITE IMAGERY INFORMATION

  • Han, Eun, Young;Chae, Gee-Ju
    • Proceedings of the KSRS Conference
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    • 2005.10a
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    • pp.286-289
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    • 2005
  • Recently, in Korea, recognizing the importance of satellite imagery and a national project, the development of satellite providing satellite imagery information of 1m high resolution has been carried out. As the application of satellite. imagery information is expanded to the national land, the environment and geographical information, etc, the necessity of integrated management of satellite imagery information increases. Unfortunately, in case of Korea, currently, the results that institutes for satellite imagery processing produce with satellite imagery have been individually managed. Integrated Management of Satellite Imagery Information project which is being promoted by ETRI (Electronics and Telecommunication Research Institute) in Korea will provide the solutions for the above mentioned problems. In this research work, we designed standard metadata for integrated management of satellite imagery information in consideration of international and national standard.

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Systematic Embedded Subsystem Development Methodology for POP System (POP 시스템 개발에 있어서의 체계적인 임베디드 서브시스템 개발방법론)

  • Jo, Young-Hyo;Han, Kwan-Hee;Choi, Sang-Hyun
    • IE interfaces
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    • v.23 no.1
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    • pp.35-47
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    • 2010
  • This paper presents a structured framework for developing the embedded subsystem, called ESDMP (Embedded Subsystem Development Methodology for POP), which is one of core components at the development of POP (Point Of Production) system. It is essential that embedded subsystem development methodology must be closely related to the general information system development methodology from the early stage of system development. Therefore, this paper investigates the PDSM (Production System Development Methodology) that is developed by Korea Technology and Information Promotion Agency for SMEs and widely utilized at the fields of POP system development, and proposes the embedded subsystem development methodology aligned with each step of PSDM. The main characteristics of proposed methodology are as follows : First, it is developed to link each step of embedded subsystem development with relating steps of PSDM from the early stage of feasibility study. Second, it provides the procedure for designing and implementing hardware and software simultaneously. Third, it includes the method of reusability for developed products and modules.

A Study on Improving Laws and Regulations for Open Access of Research Papers from National Research and Development Projects (국가R&D 논문성과물의 오픈액세스를 위한 법규 개선방안)

  • Cha, Mikyeong;Song, Kyeong-Jin;Kim, Na-Young
    • Journal of the Korean Society for Library and Information Science
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    • v.51 no.1
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    • pp.147-174
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    • 2017
  • Open Access (OA) policy to scholarly publications from publicly funded research goes toward making a law at the national level. The purpose of this study is to suggest the ways of improving laws and regulations for OA of scientific publications from national R&D projects. For that purpose, this study analyzed cases of USA, Spain, Germany, and France which have already legislated OA related laws and regulations. Based on the results, the followings were proposed: 1) to unify definitions of research outcomes and to include research papers to the definition. 2) to strengthen OA obligations by amending "Act On The Performance Evaluation And Management Of National Research And Development Projects, Etc." by to specify related articles about submission, making public, enrolling, deposition, and possession of research papers, and 3) to revise administrative rules and "Regulations on Management of National Research and Development Projects" which common to all administrative according to amending the law.