• Journal of Plant Biotechnology
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• v.33 no.4
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• pp.233-236
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• 2006

Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.

• Physical Therapy Korea
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• v.22 no.2
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• pp.30-40
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• 2015

Robot-assisted rehabilitation therapy has been used to increase physical function in post-stroke patients. The aim of this meta-analysis was to identify whether robot-assisted gait training can improve patients' functional abilities. A comprehensive search was performed of PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), Physiotherapy Evidence Database (PEDro), Academic Search Premier (ASP), ScienceDirect, Korean Studies Information Service System (KISS), Research Information Sharing Service (RISS), Korea National Library, and the Korean Medical Database up to April, 2014. Fifteen eligible studies researched the effects of robot-assisted gait training to a control group. All outcome measures were classified by International Classification of Functioning, Disability, and Health (ICF) domains (body function and structures, activity, and participation) and were pooled for calculating the effect size. The overall effect size of the robot-assisted gait training was .356 [95% confidence interval (CI): .186~.526]. When the effect was compared by the type of electromechanical robot, Gait Trainer (GT) (.471, 95% CI: .320~.621) showed more effective than Lokomat (.169, 95% CI: .063~.275). In addition, acute stroke patients showed more improvement than others. Although robot-assisted gait training may improve function, but there is no scientific evidence about the appropriate treatment time for one session or the appropriate duration of treatment. Additional researchers are needed to include more well-designed trials in order to resolve these uncertainties.

• Journal of the Ergonomics Society of Korea
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• v.31 no.1
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• pp.77-83
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• 2012

Objective: The aim of this paper is to introduce a method that can measure and analyze the judgment gaps between service providers and customers. Background: It is important to understand the good service that service providers and customers are thinking. If there is judgment gap between service providers and customers, it would cause an unsatisfactory service. The judgment gap should be thoroughly investigated for a good service design. Method: Lens model is a human decision making model that was proposed by Brunswick(1952). This study indicates whether the Lens model can be applied to analyze judgment gaps between service providers and customers. As a case study, a library lending service was selected. 5 librarians and 15 customers participated in the experiment that investigates their judgments on a good service. The obtained data were analyzed by a modified lens model. Results: Cue weighting policies of consumers and service providers were similar, except that consumers gave higher weight on tangibility than service providers. Service providers and consumers had a good knowledge on the service quality, but they could not well apply the knowledge to judge it. Conclusion: The lens model may be used to analyze judgment gaps between service providers and consumers in the other service areas. The decision cues that were used in this study can be changed, depending on the characteristics of the target service. Application: The method that is proposed in this study may help to investigate and analyze both consumers' and service providers' judgments on a variety of services.

• Journal of Digital Convergence
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• v.13 no.8
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• pp.51-57
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• 2015

Digital braille music is an indispensible instrument for the blind persons to enjoy music and to proceed the more professional music education. Producing braille music requires professionals such as braille translators thereby it costs money for an individual to order braille music. Therefore, public libraries involve in producing and distributing braille music. Current status of digital braille music in Korea has been far from the fulfillment with the unmet needs of the blind people for diversity in their music preferences. This paper analyses the present condition of braille music possession in Korea National Library. Based on the analysis, policy proposals has been proposed.

• Journal of Korea Game Society
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• v.15 no.6
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• pp.77-88
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• 2015

This paper made a comparative study on domestic and international game archives that preserve and study the history of games. Based on that, it suggests the structure to classify and build a database to establish a history museum of games. The objective to gather and preserve history of games is mainly in order to build a baseline for ludology studies, but not solely for that reason. This study proposes the series of procedures of gathering, preserving, classifying, displaying and referencing digital games according to the academic field of Media Archaeology.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.5
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• pp.932-940
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• 2011

This study describes computational simulation results in 2-dimensional and 3-dimensional space concerning large scale gap test(LSGT) by using commercial hydrocode such as AUTODYN and LS-DYNA to analyze the detonation phenomenons of high explosives. To consider the possibilities of LSGT simulation, we used Lee - Tarver reaction rate model of PBX-9404 and Comp-B which were implemented AUTODYN's material library. Also we have tried the diverse numerical schemes such as Lagrangian, Eulerian and ALE(Arbitary Lagrangian Eulerian), SPH(Smoothed Particle Hydrodynamics) in LSGT simulations. After LSGT simulations, we compared the simulation results with published results to verify the LSGT simulations. According to the LSGT simulations, we have concluded as follows. In 2-dimensional and 3-dimensional space, Lagrangian solver provided the most reliable results based on analysis time and accuracy. When using two hydrocodes in 2-dimensional space, the simulation results are almost same except one explosive model. We have verified the modeling method and simulation results of the LSGT by using the commenrcial hydrocode in this study.

• Journal of Information Management
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• v.20 no.2
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• pp.1-29
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• 1989

BLAISE, a information communication system England, has been developed steadily and expanded a network to the European countries, which reflects recognition of library at national level. In Japan, NACSIS has been established by a financial support from Ministry of Education and very active in networking throughout the nation. The development policy of their countries. In this study it is introduced a new concept, bibliographical information network and also suggested that bibliographical information system in Korea should be developed by expansion of KOR-MARC database.

• The Journal of the Korea institute of electronic communication sciences
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• v.19 no.2
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• pp.467-472
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• 2024

Multiple sequence alignment (MSA) is a method of collecting and aligning multiple protein sequences or nucleic acid sequences that perform the same function in various organisms at once. clustalW, a representative multiple sequence alignment algorithm using BioPython, compares the degree of alignment by column position. In addition, a web logo and phylogenetic tree are created to visualize conserved sequences in order to improve understanding. An example was given to confirm the differences between humans and other species, and applications of BioPython are presented.

• Journal of Information Management
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• v.40 no.1
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• pp.157-181
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• 2009

As the information technology is evolved and the human genome project is finalized over the world, the Bioinformatics - the integration of abundant Biological science and information technology - has shown up and is continuously being advanced. Together with the evolution of Bioinformatics, the websites dealing with Bioinformation have been set up to provide relevant information to the Bioscientists. Among the numerous global websites, the preferred websites by the majority of domestic Bioscientists are BRIC (Biological Research Information Center) of POSTECH(Pohang University of Science and Technology) in Korea, CCBB(Center for Computational Biology and Bioinformatics) of KISTI(Korea Institute of Science and Technology Information), KOBIC(Korean Bioinformation Center) of KRIBB(Korea Research Institute of Bioscience and Biotechnology), NCBI(National Center for Biotechnology Information) in USA, EBI(European Bioinformatics Institute) in Europe and DDBJ(DNA Data Bank of Japan) in Japan. In this paper, the comparative analysis was executed by investigating contents status and functions of the above-mentioned 6 websites. In addition, questionnaire survey of Bioscience Researchers' utilization status and their needs to those 6 websites was conducted.

• Journal of The Korea Institute of Healthcare Architecture
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• v.15 no.4
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• pp.13-21
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• 2009

Healthcare design is always challenged to come up with the rapid changes in healthcare system and industry as well as complicated planning requirements. This thesis aims to grab good instructions from Louis I Kahn who designed more than 15 healthcare projects during his entire life, from the winning project of the student competition in 1920 to Ayub hospital in Dhaka, Bangladesh after his death. His many hospital projects are deeply related to his famous projects such as Yale Art gallery, Philips Exeter Library, and Sher-E-Bangla Nagar national capital of assembly hall. But, it is not easy to find the researches about his hospital projects among many books and theses about him. Thus, this thesis investigates them and their contextual and historical relationships in order to find the way to apply his knowledge and philosophy about healthcare design to the modern hospital projects. We expect that it will promote more following researches for his healthcare projects.