• Title/Summary/Keyword: National Growth

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Cultures of Ginkgo biloba, Effect of Nutritional and Hormonal Factors on the Growth of Cultured Cells Derived from Ginkgo biloba

  • Jeon, Mee-Hee;Sung, Sang-Hyun;Jeon, Soon-wha;Huh, Hoon;Kim, Jin-woong;Kim, Young-Choong
    • Archives of Pharmacal Research
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    • v.16 no.3
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    • pp.244-250
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    • 1993
  • Calli and suspension cultures were obtained following inoculation of the explant from leaves of Ginkgo biloba L on the supplemented MS basal medium. The obtained calli and suspension cultured cells were able to produce detectable amounts of ginkgolides which are known as natural specific PAF antagonists. The production of ginkgolides in the calli and suspension cultured celles were identified using GC/MS, GC and HPLC with authentic ocmpounds. Since the production of ginkgolides A and B the calli and suspension cultured cells had been confirmed, effects of types and concentration of plant growth regulators, media and illumination on the induction and growth of the callus were studied. The concentrations of growth regulators for optimal callus were studied. The concentrations of growth regulators for optimal callus induction were studied. The concentrations of growth regulators for optimal callus induction were 1.0 to 2.0 mg/L for NAA and o.1 mg/L for kinetin. The growth of the Callus seemed to be more simnultaed with the combination of NAA and kinetin than NAA and BA with illumination at all concentration ranges of 1.0 to 4.0 mg/l for NAA and o.1 to 1.0 mg/L for kinetin or BA studied. Amogn 8 different media used, the induction rate of callus on Anderson, Eriksson, and Shenk and Hildebrant at 4 weeks after the innoculation was almost the same as that of MS. However, callus was rarely induced on Heller or White medium. Suspension cultures were easily initiated with 3 g of callus (fresh weight) derived from ginkgo leaves on supplemented MS medium. A typical growth curve of suspension cultured cells could be obtained by measuring the fresh weight of the suspension cultured cells at every 3 days. To improve the growth of suspension cultured cells of ginkgo, effects of concentrations of NAA, sucrose, phosphate ions and molar ratio of $NH_{4}^+\;to\;NO_{3}^-$ ions in the culture medium were studied. The maximum growth of the cells was achieved when the culture medium contained 1.0 mg/L of NAA, 30 g/L sucrose, 1.75 mM phosphate ions and 1:5 molar ratio of $NH_{4}\;to\;NO_{3}^-$ ions.

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A Study on the Effect of Plants Growth on Eco Wood Pots (국산 낙엽송으로 제조한 에코우드포트(Eco Wood pots)의 식물 생장 효과에 관한 연구)

  • Oh, Geun Hye;Kim, Hee-jin;Yang, Seong-min;Nam, Jeong Bin;Kang, Seog-goo
    • Journal of the Korea Furniture Society
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    • v.29 no.1
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    • pp.18-23
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    • 2018
  • The purpose of this study was to develop wood pots for create Optimum environment of plant growth using unused wood. to prove this, we examined the effects of cycle of water supply and nutrient concentration in wood pots on plant germination rate and growth factors (leaf number, stem diameter and length). The results are as follows. 1) The growth rate was higher at once of 2 days watering period. This suggests that the growth of the plants was better than that of the less water because the larch pots itself has the water retention capacity inside. 2) Germination rate and growth rate were better than other treatment groups when the concentration of nutrient solution was 0.5%. 3) Nitrogen, available phosphoric acid, and potassium showed higher contents than the nutrient - treated soil at 0.5% concentration of nutrient solution. This indicates that the nutrient solution absorbed from the larch affected the soil and plant growth in the inside.

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Effects of Soil Moisture Stress at Different Growth Stage on Growth, Yield and Quality in Rice

  • Park, Hong-Kyu;Choi, Weon-Young;Kang, Si-Yong;Kim, Young-Doo;Choi, Won-Yul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.2
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    • pp.143-148
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    • 1999
  • Soil moisture condition is an important limiting factor in growth and yield in rice culture. The purpose of this study was to compare the influence on the growth, yield and Quality of rice subjected to soil moisture stress (SMS) at different growth stages. Ajaponica rice cultivar, Dongjinbyeo, was cultured under flooded conditions in a plastic container filled with silty loam soil. The container was subjected to SMS until the initial wilting point (IWP) coincided with about 10% in soil moisture content and about-200 kPa in soil matric potential, and was then irrigated again, at 6 and 5 of main growth stage in 1996 and 1997, respectively. At maturity, the plant height, tiller number, leaf area and top dry weight were decreased more in SMS treatments at the early stage than the late stage. The averaged yield index of SMS to control in both years was lowest at meiosis (62.5%), which primarily resulted from lower percent ripened grain and 1,000 grain weight, and second' reduced the spikelet number per panicle and panicle number per hill, and followed at tillering stage (68.5%) which resulted from the lower production in tiller number and top dry matter during and after SMS treatment. The percent-age of read rice in SMS plants varied with the treatment stage as order of lower at meiosis (44.0%), heading (53.9%), panicle initiation (70.1%), tillering (72.1%), ripening (75.8%) and 5 days after transplanting (DAT) (79.0%). Protein content in brown rice was slightly larger in SMS at late growth stage than the control, while the contents of fat and ash differed very little between SMS and control. Contents of Mg and K and Mg/K in brown rice with SMS were lower at some treatment stages such as at ripening or panicle initiation.

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In Vitro Growth-inhibiting Effects of Leaf Extracts from Pinus Species on Human Intestinal Bacteria

  • Cho, Seok-Hwan;Jeon, Ho-Joung;Han, Yu-Kyung;Yeon, Seong-Hum;Ahn, Young-Joon
    • Journal of Applied Biological Chemistry
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    • v.42 no.4
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    • pp.202-204
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    • 1999
  • Methanol extracts of leaves from 15 Pinus species belonging to the family Pinaceae were tested for their in vitro growth-inhibiting activities against 10 bacteria commonly found in the gastrointestinal tracts of human, using impregnated paper disk methods. The inhibitory activities varied with both bacterial strain and Pinus species used. At a concentration of 10 mg/disk, a clear growth inhibition was produced from the extracts of Pinus armandii, P. banksiana, P. bungeana, P. densiflora, P. rigida, and P. thunbergii against Clostridium perfringens, whereas all Pinus samples revealed weak or little growth-inhibiting activity against Escherichia coli, Bacteroides fragilis, and Staphylococcus aureus. At 5 mg/disk, the extracts of P. banksiana and P. thunbergii exhibited potent growth inhibition toward C. perfringens. All the extracts except the one from P. densiflora did not adversely affect growth of Bifidobacterium adolescentis, B. longum, B. bifidum, B. breve, B. animalis, and Lactobacillus casei. The growth-inhibiting activity was more pronounced in C. perfringens, as compared to the lactic acid-producing bacteria. These results may be an indication of at least one of the pharmacological activities of these Pinus species.

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Estimation of Highland Kimchi Cabbage Growth using UAV NDVI and Agro-meteorological Factors

  • Na, Sang-Il;Hong, Suk-Young;Park, Chan-Won;Kim, Ki-Deog;Lee, Kyung-Do
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.5
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    • pp.420-428
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    • 2016
  • For more than 50 years, satellite images have been used to monitor crop growth. Currently, unmanned aerial vehicle (UAV) imagery is being assessed for analyzing within field spatial variability for agricultural precision management, because UAV imagery may be acquired quickly during critical periods of rapid crop growth. This study refers to the derivation of growth estimating equation for highland Kimchi cabbage using UAV derived normalized difference vegetation index (NDVI) and agro-meteorological factors. Anbandeok area in Gangneung, Gangwon-do, Korea is one of main districts producing highland Kimchi cabbage. UAV imagery was taken in the Anbandeok ten times from early June to early September. Meanwhile, three plant growth parameters, plant height (P.H.), leaf length (L.L.) and outer leaf number (L.N.), were measured for about 40 plants (ten plants per plot) for each ground survey. Six agro-meteorological factors include average temperature; maximum temperature; minimum temperature; accumulated temperature; rainfall and irradiation during growth period. The multiple linear regression models were suggested by using stepwise regression in the extraction of independent variables. As a result, $NDVI_{UAV}$ and rainfall in the model explain 93% of the P.H. and L.L. with a root mean square error (RMSE) of 2.22, 1.90 cm. And $NDVI_{UAV}$ and accumulated temperature in the model explain 86% of the L.N. with a RMSE of 4.29. These lead to the result that the characteristics of variations in highland Kimchi cabbage growth according to $NDVI_{UAV}$ and other agro-meteorological factors were well reflected in the model.

Transprt Phenomena in Bulk Crystal Growth Processes

  • Lan, C.W.
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1998.06a
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    • pp.29-33
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    • 1998
  • Transport phenomena paly an inmportant role in bulk crystal growth processes. During crystal growth, the control of the growth front and the dopant concentration is crucial to crystal quality. The growth feasibility in thus determined by the heat transfer controlling the interface convexity and by the mass transfer controlling the constitutional supercooling. Through numerical modeling, a thorough understanding of the growth processes is possible, which in turn is a key to process improvenment. In this paper, we will summarize some work dine in my laboratory, both numerical and experimental, to illustrate the importance of understanding the transport phenomena during crystal growth processes.

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Effects of Arbuscular Mycorrhizal Fungi and Soil Conditions on Crop Plant Growth

  • Kim, Sang Joon;Eo, Ju-Kyeong;Lee, Eun-Hwa;Park, Hyeok;Eom, Ahn-Heum
    • Mycobiology
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    • v.45 no.1
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    • pp.20-24
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    • 2017
  • We investigated the effects on various crops of inoculation with species of arbuscular mycorrhizal fungi (AMF) in soils from different sources and selected AMF species suitable for domestic environment-friendly farming. Effects on plants varied with the AMF species used. In carrot, Scutellospora heterogama, Acaulospora longula, and Funneliformis mosseae had a positive effect on growth of the host, whereas AMF had only weak effects on the growth of red pepper and leek. AMF inoculation had positive effects on the growth of carrot and sorghum. The results of this study indicate the nature of the relationship between soil, plants, and AMF; this study therefore has important implications for the future use of AMF in environment-friendly agriculture.

Optimal Culture Conditions for in vitro Propagation of Orostachys japonicus and Enhancement of Polysaccharide Production

  • Kim, Won-Jung;Kang, Young-Min;Park, Dong-Jin;Huh, Gyung-Hye;Lee, Byung-Hyun;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.129-134
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    • 2004
  • Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. The highest growth yield was achieved in 1/2 MS medium, while the lowest growth yield was obtained in 4 MS medium. The patterns of polysaccharide formation were a little similar in all cases, but on MB5 medium, the po]ysaccharide contents of plant were higher than others. Among the modified nitrate levels, effective growth level were obtained in 1/4 N and 1/2 N. High contents of polysaccharide were obtained in 4 N. Different concentration of potassium and calcium did not improve the growth and polysaccharide production. The micropropagated shoots were successfully acclimatized artificial soils.

Up-regulation of Early Growth Response-1 Expression by Endoplasmic Reticulum Stress

  • Han, Song-Yi;Kwon, Ki-Sang;Yun, Eun-Young;Goo, Tae-Won;Kwon, O-Yu
    • Biomedical Science Letters
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    • v.13 no.2
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    • pp.157-160
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    • 2007
  • Endoplasmic reticulum (ER) plays formation of disulfide bonds and proper folding of secretory proteins. Cellular responses to ER stress enhances the stress-activated kinase pathway and the induces a lot of immediate-early genes. Among of them, the early growth response-1 (Egr-1), a transcription factor, which plays an important role in cell growth, development, differentiation, apoptosis and various types of injury. For that reason, we have tested the expression of Egr-1 against ER stress inducible drugs (tunicamycin, DTT, A23187 and BFA) to understand what kind of aspect occurred by ER stresses.

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Asymbiotic seed germination and in vitro propagation condition in Calanthe discolor Lindl.

  • Kwon, Huyk Joon;Shin, So Lim;Bae, Kee Hwa;Kim, Soo-Young
    • Journal of Species Research
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    • v.6 no.1
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    • pp.68-75
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    • 2017
  • We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LEDs lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LEDs light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.