• Molecules and Cells
• /
• 제37권3호
• /
• pp.241-247
• /
• 2014

Sudden cardiac death (SCD), which is primarily caused by lethal heart disorders resulting in structural and arrhythmogenic abnormalities, is one of the prevalent modes of death in most developed countries. Myocardial ischemia, mainly due to coronary artery disease, is the most common type of heart disease leading to SCD. However, postmortem diagnosis of SCD is frequently complicated by obscure histological evidence. Here, we show that certain mRNA species, namely those encoding hemoglobin A1/2 and B (Hba1/2 and Hbb, respectively) as well as pyruvate dehydrogenase kinase 4 (Pdk4), exhibit distinct postmortem expression patterns in the left ventricular free wall of SCD subjects when compared with their expression patterns in the corresponding tissues from control subjects with non-cardiac causes of death. Hba1/2 and Hbb mRNA expression levels were higher in ischemic SCD cases with acute myocardial infarction or ischemic heart disease without recent infarction, and even in cardiac death subjects without apparent pathological signs of heart injuries, than control subjects. By contrast, Pdk4 mRNA was expressed at lower levels in SCD subjects. In conclusion, we found that altered myocardial Hba1/2, Hbb, and Pdk4 mRNA expression patterns can be employed as molecular signatures of fatal cardiac dysfunction to forensically implicate SCD as the primary cause of death.

• 한국치위생학회지
• /
• 제19권3호
• /
• pp.449-449
• /
• 2019

• 분석과학
• /
• 제35권2호
• /
• pp.82-91
• /
• 2022

Grayanotoxin-contaminated honey exhibits toxicity. In this study, a reliable and sensitive liquid-chromatography-tandem-mass-spectrometric method (LC-MS/MS) was developed and validated for the quantitation of grayanotoxin I and grayanotoxin III in honey. The grayanotoxins were extracted from honey via solid phase extraction and separated on a biphenyl column with a mobile phase consisting of 0.5 % acetic acid in water and methanol. Mass spectrometric detection was performed in the multiple-reaction monitoring mode with positive electrospray ionization. The calibration curve covered the range 0.25 to 100 ㎍/g. The intra- and inter-day deviations were less than 10.6 %, and the accuracy was between 94.3 and 114.0 %. The validated method was successfully applied to the determination of grayanotoxins in mad honey from Nepal. The concentrations of grayanotoxin I and grayanotoxin III in 33 out of 60 mad honey samples were 0.75 - 64.86 ㎍/g and 0.25 - 63.99 ㎍/g, respectively. The method established herein would help in preventing and confirming grayanotoxin poisoning.

• 대한수의학회지
• /
• 제63권3호
• /
• pp.21.1-21.6
• /
• 2023

The increasing prevalence of toxic substance-exposure in pets in South Korea endangers the health and safety of numerous companion animals, and has become a cause for concern. Notably, the annual incidence of forensic analysis in pets has increased by more than 150% in South Korea, mainly in populous regions such as Seoul, Incheon, and Gyeonggi. In response to this growing issue, veterinary forensic examinations were conducted on 549 dogs and cats from 2019 to 2022. This study revealed the presence of various toxic substances, including pesticides, insecticides, and drugs such as analgesics, anesthetics, antidepressants, and muscle relaxants, in pets. Among the 38 different toxins identified in pets, coumatetralyl, methomyl, terbufos, and buprofezin were the most frequently detected. In this study, toxic substances for pets were identified based on the "toxic agent list for humans," developed by the National Forensic Services, because no list of toxic agents for animals currently exists and data regarding potentially toxic substances for dogs and cats is limited. This is one of the limitations of this study, and necessitates the establishment of a toxic agent list for animals. Continued monitoring and research is also recommended to reveal the incidence, causes, and solutions of toxicity in animals.

• 한국안전학회지
• /
• 제27권4호
• /
• pp.38-42
• /
• 2012

This paper describes electrical fire on residential environment such as apartment and detached house caused by defect of interior wiring. We carried out experimental study on short circuit characteristics by the interior wiring length. We were measured arc current, arc energy and interrupting time of earth leakage current circuit breaker(ELB), when an interior wiring break out short circuit in residential environment. From the experiment results, the longer of the interior wiring, the magnitude of arc current decreased and the interrupting time of ELB increased. When applied the A maker's ELB, the strength of arc current and interrupting time of ELB was 254 A and 245 ms respectively at 30 m interior wiring length. In 3 m interior wiring length, arc current and interrupting time was 716 A and 4.24 ms respectively. Arc energy was dependent on the magnitude of arc current and the interrupting time of ELB, the longer the interrupting time, arc energy increasing. In this paper, minimum arc energy was 277 J using C maker's ELB and 3 m interior wiring length(arc current 283 A, interrupting time of breaker 6.28 ms). Therefore in the residential environment, short circuit caused by defect of the interior wiring lead to electrical fire.

• 한국안전학회지
• /
• 제26권2호
• /
• pp.42-47
• /
• 2011

Going underwater is supposed to begin with the history of human beings. At first it was confined to relatively shallow level, less than several meters by holding breath. Recently, deep level diving has been necessary for such purpose as construction, maritime salvage, military operations, research and sports by using SCUBA(self-contained underwater breathing apparatus) equipment. As one goes down into water, the pressure on the diver is increased due to water pressure with depth, usually 1 atm for each 10 m water level. In deep water, mixed gas or nitrox(EAN, enriched air nitrox) could be applied for the divers lest they should get disease due to high pressure. Of these, the former is usually composed of oxygen and inert gas like helium or hydrogen, the latter contains higher oxygen content than that in normal air in which the oxygen concentration is designated by the character "EAN" followed by vol. % of oxygen, for example, "EAN 40" contains 40% of oxygen. In this case, a victim was found at the 39 m below the sea surface breathing air and nitrox in cylinder wrongly marked as EAN 36, which was analyzed to contain 63% of oxygen by GC/TCD. The cause of death could not be exactly related with the oxygen content in the nitrox cylinder, because the accurate depth for the victim to dive was not known, even though the victim was just found at the depth of 39 m. However, the wrongly marked nitrox could be believed to be the main cause of the death at the depth unless there happened any other accident except that during diving.

• 대한기계학회논문집A
• /
• 제38권4호
• /
• pp.451-457
• /
• 2014

최근에는 법공학 분야에 구조해석 및 구조-유동 연성해석을 이용한 다양한 시뮬레이션 기법을 활용하여 안전사고 및 재난사고에 대한 법적 책임문제를 해명하고, 보다 정확한 원인분석을 통해 원인을 규명하고 있는 추세이다. 본 연구에서 다룬 플레이트 거더교 사고는 교각과 교대 사이의 기존 교각에 대하여 확장 교각을 신설하던 중 방호벽 콘크리트 타설이 완료되는 시점에 전도 추락되어 발생되었다. 본 사고는 설계와 다른 시공 작업과 설계 시 고려되지 않은 추가 하중 등으로 인해 확장 교량에 부반력이 발생하여 구조적 불안정을 가져와 붕괴된 사고로 MIDAS 구조 해석을 통해 설계와 실제 시공 시 작업 하중에 대한 구조적 안정성을 비교 평가하여 정확한 공학적 사고의 원인을 규명하고자 한다.

• 대한기계학회논문집A
• /
• 제37권11호
• /
• pp.1415-1421
• /
• 2013

최근에는 이러한 법공학 분야에 유한요소법 및 유한체적법을 이용한 다양한 시뮬레이션 기법을 활용하여 안전사고 및 재난사고에 대한 법적 책임문제를 해명하고 보다 정확한 원인분석을 통해 원인을 규명하고 있는 추세이다. 본 연구에서는 ADINA 구조해석을 이용하여 단경교 거더교 붕괴사고에 관한 좌굴 원인분석을 수행하였다. 본 연구에서 다루는 사고는 단경교 거더교 신축 중 상현 슬래브 구성을 위한 콘크리트 타설 중 교량이 붕괴된 사고로 원설계 공법에서 가설 공법이 변경되었고 상현 콘크리트 블록 및 격벽 콘크리트 등을 미시공한 상태에서 그 다음 단계인 상현 슬래브 콘크리트 타설을 진행함으로써 구조적 불안정을 가져와 거더가 좌굴이 발생하여 붕괴된 사고이다. 사고 재현 실험이 불가한 경우 F.E.M 을 이용한 구조안정성 비교 평가는 정확한 공학적 사고의 원인을 규명하는데 효과적인 방법이다.

• 대한수사과학회지
• /
• 제2권2호
• /
• pp.50-66
• /
• 2007

사건 사고현장에서 가장 먼저 접근하는 초동조치 활동은 원인을 규명하는데 결정적인 역할을 한다. 법의학적 지식이 부족한 자가 최초현장에 접근하여 결정적인 사건해결 증거를 멸실케 한다면 억울한 죽음은 물론이고 무고한 혐의자를 발생시키며 유사범죄의 발생을 막을 수 없을 것이다. 본 연구는 119구급대 응급구조사에 대한 법의학교육의 필요성을 제시하고자 전국 대학(교) 응급구조학과 및 2급 양성기관의 법의학교육 실태분석을 위한 조사를 시행하였으며, 119구급대원 중 1 2급 응급구조사 307명(1급 43%, 2급 57%)을 대상으로 설문조사를 실시하였다. 그 결과 업무 중 범죄와 관련된 경험 94.8%, 죽음(변사체)의 경험이 91.9%였고, 이러한 현장에 92.8%가 경찰보다 먼저 현장에 도착하였다. 응답자의 72%는 법의학지식이 부족하여 업무 중에 어려움을 겪은 경험이 있었고, 수사경찰관과 문제, 법적증언, 유가족과의 문제 등을 경험하였다. 90.9%는 응급구조사의 업무수행에 법의학관련 지식이 도움이 된다고 긍정적으로 밝혔으며, 법의학교수에 의해 정기적인 교육을 희망하고 있었다. 응급구조사들의 법의학적 검시관련 지식을 알아보기 위해 국가 및 지방자치단체가 억울한 죽음이 없도록 감시하고, 정책적 차원에서 법의학적인 검시가 필요한 것에 대한 질문에 1급 2급 응급구조사 두 군 모두 20개 항목 중 각각 8.86개와 7.99개만 인식하고 있어, 법의학교육이 절실히 필요한 것으로 나타났다. 따라서 법의학은 응급구조사에게 단순히 범죄와 관련된 상황에서만 필요한 것이 아니라 각종 대량재해 교통사고 보험관련 사고 산업재해 등의 죽음과 관련된 법률적 문제를 해결하는 데에도 연관되어 있다. 신고체계가 다원화 되어 있는 우리나라 현실에서 119구급대의 응급구조사들은 의학적 판단에 기초한 신속하고 정확한 응급처치와 더불어 현장을 보존하고 결정적 사건해결의 물적 증거 보존을 위한 법의학적 지식을 가지기 위해서는 체계적인 법의학교육이 시행되어져야 할 것이다.

• 한국동물위생학회지
• /
• 제40권4호
• /
• pp.277-280
• /
• 2017

A 3 year-old black goat was presented to Animal and Plant Quarantine agency for diagnosis in June, 2017. She was intaken feed with Rhododendron schlippenbachii the day before death. The clinical signs included loss of appetite, lethargy, hypersalivation, astasia, yelling. At necropsy, foamy discharge were observed in the airway. Histologically, foreign body, eosinphil and macrophges was observed in alveolar lumen of lung. Grayanotoxin derived from Rhododendrons was detected in ruminal contents. Based on the pathological and toxine examination, we diagnosed this case as grayanotoxin poisoning in a black goat.