• Journal of fish pathology
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• v.21 no.3
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• pp.209-217
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• 2008

A disease with mass mortality characterized exophthalmia and abdominal distension by ascites occurred in farmed starry flounder, Platichthys stellatus in Pohang since October of 2007. Severe necrosis and infiltration of inflammatory cells were observed in tissue histopathological sections from liver, kidney, spleen and heart of the diseased fish. The catalase-negative and gram positive cocci with -hemolysis were isolated from moribund fish. Analysis of the API profiles and the multiplex PCR of the isolates showed that strains were identified as Streptococcus parauberis. In API profiles, S. parauberis isolated from starry flounder differed from those of olive flounder, Paralichthys olivaceus to the test of arginine dihydrolase, D-mannitol, D-sorbitol and α-lactose. And the strains, PH0710 and PH0711, were strongly lethal for starry flounder (LD50 ranging from 4.2×103~4.2×102 cfu/fish at 7 days after intraperitoneally inoculation). This is the first report on a S. parauberis infection in starry flounder.

• Journal of Environmental Science International
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• v.22 no.6
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• pp.761-771
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• 2013

The species composition and variation of marine plants at Saguenjin in the east coast of Korea were investigated monthly from October 2008 to December 2009. As a result, 92 species of marine plants identified, 91 species were seaweeds (7 green algae, 22 brown algae, 62 red algae) and 1 were sea grass. Dominant species in importance value were melobesioidean algae, Ulva pertusa and Phyllospadix iwatensis. The vertical distribution of algae were characterized by melobesioidean algae, U. pertusa at 3 m depth, melobesioidean algae, P. iwatensis and U. pertusa at 5 m depth and melobesioidean algae, Chondrus ocellatus and Prionitis cornea at 10 m depth.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.1
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• pp.56-64
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• 2012

Polychaete community structure and its spatial distribution was investigated in 2008 from inshore and offshore of Lake Shihwa, Korea, in order to evaluate status of the benthic environment according to construction of the dike and the water gate. In the present study, the number of species, density, and diversity of polychaete community in inshore was significantly different from those in offshore. The density of polychaete community in offshore increased with the number of species whereas the diversity in inshore increased with the number of species. Dominant species in offshore were 13 species, higher than 1% of the total polychaete individuals. Heteromastus filiformis, known as the most dominant species before the construction of the dike, was the most dominant species in offshore, which collectively account for 54% of the total polychaete individuals. In inshore, the seven species were dominant, higher than 1% of the total individuals. Lumbrineris longifolia, Polydora sp., Capitella capitata, Pseudopolydora paucibranchiata, known as pollution tolerant species, contributed to higher than 75% of the total individuals in inshore. Multivariate statistical analyses, non-metric multidimensional scaling, showed apparent difference in polychaete community structure between inshore and offshore, and also difference between inner sites and outer sites of inshore around the water gate. Sediment characteristics (total organic carbon and nitrogen, ignition loss, and acid volatile sulfide) measured in this study also supported to this result. Therefore, this indicates that the offshore provides better benthic environments for polychaete habitation than the inshore, and the inshore around the water gate shows improving benthic environment, compared to the inner inshore.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.6
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• pp.471-477
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• 2008

Development of egg, larvae and juveniles for the Pacific mackerel, Scomber japonicus are described following natural fertilization in the indoor tank of $25^{\circ}C$ water temperature. Following a routine hormone treatment technique for the brood stock, male and female mackerels were artificially matured by intramuscular injections of LHRHa at a dosage of $400{\mu}g/kg$ body weight (BW)+Domperidone at a dosage of $4{\mu}g/kg$ body weight (BW) to induce maturation in a separate aquarium and induced natural spawning. Fertilized eggs were ca. 1.0 mm in diameter; spherical in shape with a single oil globule; pelagic and non-adhesive. Hatching occurs 41 hours after fertilization at $23-24^{\circ}C$. The newly hatched larvae was 3.03 mm in average total length (ATL), the mouth and anus were not open, oil globule located in posterior end of yolk sac, and preanal length was 42.8% of TL. The larvae measuring 2.89 mm ATL, almost absorbed yolk sac and oil globule material in 2 days after hatching, in which the mouth and anus were open. Melanophores, branch or star in shape were observed on the top of head, peritoneal region and along the ventral contour. In 13 days after hatching, the larvae was 6.88 mm ATL, its posterior end of notochord began to flex upward, finfold of caudal fin appeared, jaw teeth were already formed. In 19 days after hatching, the larvae was 7.71 mm ATL completed only caudal fin rays (9+8), and preanal length was 49.4% of TL. In 37 days after hatching, the larvae was 27.4 mm ATL already completed all the fins, and preanal length was 59.9% of TL.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.917-922
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• 2013

Changes in fishing ground of the large pair trawler were investigated by year, month and species off Korean waters. We used catch data by sea-block lat.${\times}$long.:$30^{\prime}{\times}30$) of the National Fisheries Research and Development Institute (NFRDI) and monthly fishing production statistics of Korean statistic information service from 1990 to 2007. In the 1990s, the demersal fish like Larimichthys polyactis, Collichthys sp, Portunus trituberculatus and Muraenesox cinereus were the dominant species, and in the 2000s, they were changed from demersal fish into pelagic fish like Scomberomorus niphonius, Scomber japonicus, Engraulis japonicus and Todarodes pacificus. Species composition of catch showed temporal variation by cluster analysis deviding into 4 period, 1990-1994, 1995-2000, 2001-2003 and 2004-2007. Fishing ground in the 2000s was decreased up to 2/3 in the 1990s, while the density of catch in the fishing ground in 2000s was higher than the 1990s.

• Journal of fish pathology
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• v.26 no.3
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• pp.129-137
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• 2013

The study surveyed infection rate of Kudoa septempunctata parasitized in the trunk muscle of olive flounder, Paralichthys olivaceus, cultured in Jeju Island and wild fish species caught in the coastal area around Jeju Island during 2012. Among 143 olive flounder that were randomly sampled from 26 different culture farms, K. septempunctata was detected in 7 fish samples (4.9%) from 4 different culture farms, showing no typical Kudoa infestation. However, K. septempunctata was not detected in olive flounder fry sampled from hatcheries and 8 species of wild fish. In addition, we compared 3 different sampling sites on trunk muscle of 7 Kudoa positive fish that included head part, tail part and entire muscle. Among 7 fish, K. septempunctata was detected in 3 fish that were sampled from head part; while 4 fish from tail part of trunk muscle. However, all 7 fish were positive when sampled from entire muscle. Thus, we suggest that it will be more efficient to use entire muscle sample than sampling partial muscle parts for detection of K. septempunctata.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.1
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• pp.67-82
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• 2008

This study was performed to investigate changes in benthic environment and macrobenthic polychaete communities in Gamak Bay where various environmental quality improvement projects have been implemented in recent years. Field surveys were carried out in February, 2005 and February, 2006 and twenty stations were selected to explore whether or not there were between-year differences in biotic and abiotic variables. Of 10 environmental variables measured, only three variables including dissolved oxygen (DO), total ignition loss (IL), acid volatile sulfide (AVS) showed significant between-year differences. Specifically, IL and AVS were, on average, 1.5 and 3 times lower in 2006 compared to those in 2005, respectively, which was more pronounced in the northern part of the bay. A total of 95 polychaete species was sampled from the two sampling occasions. Between-year differences in the number of species, abundance, and diversity were varied from place to place. In the northern part of the bay, fewer species were found in 2006 rather than in 2005, but diversity increased in 2006 due to the reduction in dominance of a few species. On the contrary, in the central part of the bay, the number of species, abundance and diversity prominently increased in 2006. In the southern part of the bay, all the biological indices maintained similarly during the two years. Dominant species in 2005 were such opportunistic or organic pollution indicator species as Lumbrineris longifolia, Capitella capitata, Mediomastus californiensis, Pseudopolydora paucibranchiata, etc. and most of them were mainly distributed in the northern part of the bay and in the proximity of it. In 2006, however, Euchone alicaudata, L. longifolia, Paraprionospio pinnata, Flabelligeridae sp., etc. were dominant and distributed mainly in the central part of the bay. Multivariate analyses showed that the whole polychaete community could be divided into 5 groups reflecting the geographical positions of the sampling stations and temporal variation particularly in the northern part of the bay. According to the results of BIO-ENV procedure, TOC (${\rho}=0.52$) and AVS (${\rho}=0.49$) as a single variable best explained the polychaete community structure. The best combination was made by such variables as TOC, AVS, sorting coefficient, and water temperature (${\rho}=0.60$). In conclusion, between-year differences in biotic and abiotic variables imply that recent efforts for the environmental improvement produced positive influences on the benthic environment of Gamak Bay, particularly the northern part of the bay.

• Development and Reproduction
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• v.14 no.2
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• pp.51-58
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• 2010

This research investigated that the effect of water temperature on egg developmental stages of largescale blackfish Girella punctata and smallscale blackfish Girella melanichthys. The required times from fertilized embryos to hatching for the G. punctata were 67.8~37.5 hrs from 15 to $21^{\circ}C$, shorter as the water temperature increased. But fertilized embryos were dead after Kuffer's vesicle appearance stage at $24^{\circ}C$ and morula stage at $27^{\circ}C$. Biological minimum temperature (starting point for embryonic development) of the egg development was estimated to be $7.6^{\circ}C$ in average. In G. melanichthys, fertilized embryos were hatched within 61.2~38.3 hr from 15 to $21^{\circ}C$, the times were shorter as the water temperature increased to hatching, but its were dead on above $24^{\circ}C$. Biological minimum temperature of the egg development was estimated to be $6.5^{\circ}C$ in average. Based upon these results, it is recommended that the range of optimum water temperature for embryonic development of G. punctata and G. melanichthys is $15{\sim}21^{\circ}C$.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.41 no.3
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• pp.188-198
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• 2005

This study was conducted to survey the catches of vertical bottom longline fisheries in the sea mount of central northern Pacific($30^{\circ}-42^{\circ}N$, $170^{\circ}-175^{\circ}E$), during the period of July 1 to August 25,2004 by commercial fishing vessel. The number of 57 test fishing was carried out in the central northern Pacific during 43 days and the total catches were 21,092.4kg as 19 fish species, CPUE/day and catches/day were 185 baskets and 490.5kg, respectively. Main fish species caught from the experimental fishing were Squalus mitsukurii (66.3%), Coelorhyrchus asperocephaius (11.7%) and Helicolenus avius (9.8%) and, average inside diameter for fish mouth was 4.0cm over. Catch ratio according to each fishing ground was the order of F, D, J, B and C. Catch ratio fur water depth was the order of 450-500m, 350-400m, 300-350m, 400-450m, 1000-1100m and 500-550m and, main species by water depth was Squalus mitsukurii for 300-400m, Etmopterus lucifer for 300-550m, Coelorhyrchus asperocephaius far 1,000m over. Catch ratio according to the kind of hooks was higher at the hook no.6 for Squalus mitsukurii and no. 5 for Etmopterus lucifer and, catch ratio by baits was higher at squid for Squalus mitsukurii, saury and eel for Helicolemus avius and saury for Etmopterus lucifer. Accordingly, it is thought that the extension of fishing hours is needed with the reduction of damage and loss for fishing gears during fishing operation.

• Journal of Environmental Science International
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• v.18 no.12
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• pp.1331-1338
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• 2009

Marine dinoflagellate Alexandrium minutum producing paralytic shellfish toxins is responsible for paralytic shellfish poisoning (PSP). To investigate its temporal distributions in Chinhae Bay where PSP occurs annually, SYBR Green I based A. minutum-specific real-time PCR probe was developed on the LSU rDNA region. Assay specificity and sensitivity were tested against related species, and its specificity was further confirmed by sequencing of field-derived samples. Ten months field survey in 2008 (a total 100 surface water samples) by using the real-time PCR probe showed that A. minutum was detected at very low densities of 1-4 cells $L^{-1}$ in May and June being spring in Chinhae Bay, Korea.