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http://dx.doi.org/10.5322/JES.2009.18.12.1331

Temporal Changes in Abundances of the Toxic Dinoflagellate Alexandrium minutum (Dinophyceae) in Chinhae Bay, Korea  

Park, Tae-Gyu (Ecology and Oceanography Division, National Fisheries Research and Development Institute (NFRDI))
Kang, Yang-Soon (Ecology and Oceanography Division, National Fisheries Research and Development Institute (NFRDI))
Publication Information
Journal of Environmental Science International / v.18, no.12, 2009 , pp. 1331-1338 More about this Journal
Abstract
Marine dinoflagellate Alexandrium minutum producing paralytic shellfish toxins is responsible for paralytic shellfish poisoning (PSP). To investigate its temporal distributions in Chinhae Bay where PSP occurs annually, SYBR Green I based A. minutum-specific real-time PCR probe was developed on the LSU rDNA region. Assay specificity and sensitivity were tested against related species, and its specificity was further confirmed by sequencing of field-derived samples. Ten months field survey in 2008 (a total 100 surface water samples) by using the real-time PCR probe showed that A. minutum was detected at very low densities of 1-4 cells $L^{-1}$ in May and June being spring in Chinhae Bay, Korea.
Keywords
Alexandrium minutum; Dinoflagellate; PSP toxins; Real-time PCR;
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