• Clean Technology
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• v.19 no.4
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• pp.459-463
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• 2013

Recent rapidly growth in nanotechnolgies is promised novel benefits through the exploitation of their unique industrial and biomedical applications. In addition, these nanomaterials and nano-consumer products have increased in quantity per year, and thus their uncontrolled release into the environment is anticipated to grow dramatically in future. Many papers for cytotoxicity of nanomaterials have been already reported, and thus government supports has funded to various research topics for nanosafety. Herein, we analyzed the importance and performance of nanosafety researches and tried to show the research direction where we have to go.

• Bulletin of the Korean Chemical Society
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• v.33 no.12
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• pp.4023-4027
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• 2012

Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (${\mu}FIC$). The $LC_{50}$ value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/L). These results confirm that the ${\mu}FIC$ approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This ${\mu}FIC$ method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty-toxicity relationships.

• Journal of the Optical Society of Korea
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• v.18 no.5
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• pp.551-557
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• 2014

In this study, we demonstrated multimodal nonlinear optical (NLO) microscopy integrated simultaneously with two-photon excitation fluorescence (TPEF), second-harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS) in order to obtain targeted cellular and label-free images in an immunofluorescence assay of the atherosclerotic aorta from apolipoprotein E-deficient mice. The multimodal NLO microscope used two laser systems: picosecond (ps) and femtosecond (fs) pulsed lasers. A pair of ps-pulsed lights served for CARS (817 nm and 1064 nm) and SHG (817 nm) images; light from the fs-pulsed laser with the center wavelength of 720 nm was incident into the sample to obtain autofluorescence and targeted molecular TPEF images for high efficiency of fluorescence intensity without cross-talk. For multicolor-targeted TPEF imaging, we stained smooth-muscle cells and macrophages with fluorescent dyes (Alexa Fluor 350 and Alexa Fluor 594) for an immunofluorescence assay. Each depth-sectioned image consisted of $512{\times}512$ pixels with a field of view of $250{\times}250{\mu}m^2$, a lateral resolution of $0.4{\mu}m$, and an axial resolution of $1.3{\mu}m$. We obtained composite multicolor images with conventional label-free NLO images and targeted TPEF images in atherosclerotic-plaque samples. Multicolor 3-D imaging of atherosclerotic-plaque structural and functional composition will be helpful for understanding the pathogenesis of cardiovascular disease.

• Clean Technology
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• v.28 no.2
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• pp.123-130
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• 2022

Nanoparticles are used in various fields such as chemistry, medicine, the environment, and information and communication. With the increasing use of engineered nanomaterials, exposure to nanoparticles is expected to increase in the workplace and the environmental media. However, while nanotechnology industries are expanding, research on the exposure assessment of nanomaterials to humans and the environment is only at a beginning stage. Especially, if nanoparticles with a size of 100 nm or less that are contained in nano-products are released unintentionally, they may pose potential risks to the human body through breathing or skin exposure. Therefore, in this work, the possibility of potential exposure of nanoparticles moving from the laboratory to the office was confirmed, and nanoparticle safety guidelines are proposed. A nano-collector was used to detect nanoparticles in the atmosphere, and through use of a scanning mobility particle sizer it was found that nanoparticle concentrations in the laboratory and the office tended to be similar. On the assumption that nanoparticles attached to a lab-coat move out of the laboratory, a lab-coat to which nanocarbon black was attached was shaken and the concentration of the remaining particles on the lab-coat determined. The results confirmed that sufficient amounts of nanoparticles attached to the lab-coat could move from the laboratory to the office along the path of a researcher; thus, safety guidelines for the handling of lab-coat nanoparticles are required.