• 한국잠사곤충학회지
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• 제50권2호
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• pp.122-125
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• 2012

본 연구는 누에 배자 발생 초기 특이 발현 유전자 프로모터를 개발하기 위한 연구의 일환으로 추진하였다. 누에 초기 및 후기 배자로 부터 분리한 mRNA를 사용하여 subtractive hybridization 분석법으로 누에 배자 발생 초기 특이 발현 유전자 4종을 선발할 수 있었다. 선발된 4종 유전자는 각각 BmNanos protein mRNA, BmNanos-P protein mRNA, BmNanos-O protein mRNA 및 BmVasa protein mRNA 유전자와 매우 높은 상동성을 보였다. 또한, 본 연구에서는 Northern hybridization 분석 및 real time PCR 분석을 통하여 배자 초기에 특이적으로 고발현하는 BmNanos-like 등 4개 선발 유전자의 발현 특성을 확인하였다. 이러한 결과는 추후 추진 할 누에 형질전환용 전이벡터의 효율성 제고를 위한 연구에 활용될 것으로 기대된다.

• 식품보건융합연구
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• 제4권4호
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• pp.18-25
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• 2018

Proteins play a key role in many functions such as metabolic activity, differentiation, as cargos and cell fate regulators. It is necessary to know about the markers involved in male fertility in order to develop remedies for the treatment of male infertility. But, the role of the proteins is not limited to particular function in the biological systems. Some of the proteins act as ion channels such as catsper and proteins like Nanos acts as a translational repressor in germ cells and expressed in prenatal period whose role in male fertility is uncertain. Rbm5 is a pre mRNA splicing factor necessary for sperm differentiation whose loss of function results deficit in sperm production. DEFB114 is a beta defensin family protein necessary for sperm motility in LPS challenged mice where as TEX 101 is a plasma membrane specific germ cell protein whose function is not clearly known u to now. Gpr56 is another adhesion protein whose null mutation leads to arrest of production of pups in rats. Amyloid precursor protein role in Alzheimer's disease is already known but it plays an important role in male fertility also but its function is uncertain and has to be considered while targeting APP during the treatment of Alzheimer's disease. The study on amyloid precursor protein in male fertility is a novel thing but requires further study in correlation to alzheimer's disease.

• 세라미스트
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• 제9권6호
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• pp.24-29
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• 2006

Electron beam physical vapor deposition (EB-PVD) process has currently been applied to thermal barrier coatings (TBCs) for aircraft engines. Due to unique columnar structure, EB-PVD TBCs have advantages in resistances to thermal shock and thermal cycle for their applications, compared to films prepared by plasma spray By the EB-PVD equipment, we successfully obtained yttria-stabilized zirconia (YSZ) layer which has columnar and feather like structure including a large amount of nano size pores and gaps. The EB-PVD technique has been developed for coating functional perovskite type oxides such as (La, Sr)MnO3. Electrode properties have been improved by interface and structural control.

• 한국재료학회지
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• 제19권12호
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• pp.657-660
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• 2009

Optical characteristics and structural changes depending on CuO content in phosphate glasses that are used in near-infrared (near-IR) filters were investigated. With phosphate glasses that contain 1-9 mol% CuO, changes in optical transmittance, optical absorption, and color coordinate were measured with a UV-VIS spectrophotometer. An XPS (X-ray photoelectron spectroscopy) analysis was performed to determine valence of copper ion that influences optical characteristics in near-IR filter glasses. Structural changes in glasses depending on CuO content were also analyzed by FT-IR (Fourier transform infrared) and Raman spectrophotometers. From the UV-VIS spectrophotometer results, strong absorption peaks at 220 & 900 nm were found and transmittance was decreased. The color coordinates of the glasses were shifted to the green color direction with CuO addition for increasing absorption of long wavelength range spectra, in spite of the amount of $Cu^{2+}$, which gives a blue color to glasses, and which was increased in XPS results. Also, structural de-polymerization of glasses with CuO addition were found by FT-IR and Raman results.

• 한국기계가공학회지
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• 제14권4호
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• pp.8-13
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• 2015

In this study, we fabricated and characterized a nanostructured surface based on a plastic injection molding with a local mold heating (LMH) system. A metal mold core with a closed packed nano convex array (CVA) was achieved by integrated engineering procedures: (1) master template fabrication by anodic aluminum oxidation (AAO), (2) nickel electroforming (NE) process, and (3) post-processing by precision machining. The nickel mold core was utilized to replicate a surface with a closed packed nano concave-array (CCA) based on injection molding using cyclic olefin copolymer (COC) as a plastic material. In particular, an LMH system was introduced to enhance transcription quality of the nano structures by delaying solidification of molten polymer near the surface of the mold core.

• Structural Engineering and Mechanics
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• 제53권2호
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• pp.355-372
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• 2015

This paper identifies the effects of infill wall existence and arrangement in the seismic response of steel frame structures. The methodology followed was based on the utilisation of overall seismic response indicators that distil the complexity of structural response in a single value hence enabling their straightforward comparative and statistical post process. The overall structure damage index after Park/Ang ($OSDI_{PA}$) and the maximum inter-story drift ratio (MISDR) have been selected as widely utilized structural seismic response parameters in contemporary state of art. In this respect a set of 225 Greek antiseismic code (EAK) spectrum compatible artificial accelerograms have been created and a series of non-linear dynamic analyses have been executed. Data were obtained through nonlinear dynamic analyses carried on an indicative steel frame structure with 5 different infill wall topologies. Results indicated the significant overall contribution of infill walls with a reduction that ranged 35-47% of the maximum and 74-81% of the average recorded $OSDI_{PA}$ values followed by an overall reduction of 64-67% and 58-61% for the respective maximum and average recorded MISDR values demonstrating the relative benefits of infill walls presence overall as well as localised with similar reductions observed in 1st level damage indicators.

• 한국진공학회지
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• 제16권1호
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• pp.74-78
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• 2007

전자 산업의 발달과 더불어 더욱 더 작은 구조물의 제작을 위한 새로운 물질공정 기술에 대한 수요가 증가하고 있다. 물질 고유의 물리적인 성질을 유지하면서 초미세 공정이 필용한 시점에서 기존의 전통적으로 사용하여 왔던 기계적인 방법과 레이저 공정은 공정 분해능의 한계와 물리화학적인 변형 때문에 최종 부품의 성능저하와 불량률을 증가시켜 많은 문제점을 내포하고 있다. 반면에 고출력 펨토초 레이저 기반물질 공정 기술은 기존 레이저 공정에 비해 열적 손상이 매우 작기 때문에 최근 많은 분야에서 큰 관심을 불러일으키고 있다. 본 해설 논문에서는 박형 실리콘 기판의 마이크로 패키징 및 게르마늄 표면 공정을 통한 나노구조체 형성에 대해 본 연구진에서 발표한 최근의 개발 내용을 중심으로 관련 연구 분야를 소개하고자 한다.

• Journal of Veterinary Science
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• 제21권1호
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• pp.13.1-13.14
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• 2020

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.