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http://dx.doi.org/10.7852/jses.2012.50.2.122

A Study on the Development of an Early Embryonic Gene of the Silkworm, Bombyx mori  

Choi, Kwang-Ho (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Goo, Tae-Won (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kim, Seong-Ryul (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Park, Seung-Won (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kim, Sung-Wan (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kang, Seok-Woo (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Sericultural and Entomological Science / v.50, no.2, 2012 , pp. 122-125 More about this Journal
Abstract
This study was aimed for a development of a useful gene promoter which has a transcript expressional specificity in the early embryonic period of the silkworm, Bombyx mori. To select a useful gene expressed in the early embryonic stage, we constructed and analyzed a PCR-base subtraction cDNA library. In subtractive hybridization analysis, we confirmed four clones as differently expressed genes(BmNanos-like, BmNanos-P, BmNanos-O, BmVasa mRNAs). Northern hybridization and real time PCR results reveled that the BmNanos-like gene promoter is suitable for the silkworm transgenic vector system. Further defined studies on molecular functions and biological roles of their promoters will give us well-fined information and its application.
Keywords
Silkworm; Embryo; Substraction cDNA library;
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