• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1096-1103
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• 2019

UCB-1 is the commercial rootstock of pistachio. Reproduction of this rootstock by tissue culture is limited by low levels of proliferation rate. Therefore, any compound that improves the proliferation rate and the quality of the shoots can be used in the process of commercial reproduction of this rootstock. Use of plant growth-promoting bacteria is one of the best ideas. Given the beneficial effects of nanoparticles in enhancement of the growth in plant tissue cultures, the aim of the present study was to investigate the effects of nanoencapsulation of plant growth-promoting rhizobacteria (using silica nanoparticles and carbon nanotubes) and their metabolites in improving UCB1 pistachio micropropagation. The experiment was conducted in a completely randomized design with three replications. Before planting, treatments on the DKW medium were added. The results showed that the use of Pseudomonas fluorescens VUPF5 and Bacillus subtilis VRU1 nanocapsules significantly enhanced the root length and proliferation. The nanoformulation of the VUPF5 metabolite led to the highest root length (6.26 cm) and the largest shoot (3.34 cm). Inoculation of explants with the formulation of the metabolites (both bacterial strains) significantly elevated the average shoot length and the fresh weight of plant compared to the control. The explants were dried completely using both bacterial strains directly and with capsule coating after the three days.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1373-1382
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• 2021

Plant growth promoting rhizobacteria (PGPR) are a group of bacteria that can increase plant growth; but due to unfavorable environmental conditions, PGPR are biologically unstable and their survival rates in soil are limited. Therefore, the suitable application of PGPR as a plant growth stimulation is one of the significant challenges in agriculture. This study presents an intelligent formulation based on Bacillus velezensis VRU1 encapsulation enriched with nanoparticles that was able to control Rhizoctonia solani on the bean. The spherical structure of the capsule was observed based on the Scanning Electron Microscope image. Results indicated that with increasing gelatin concentration, the swelling ratio and moisture content were increased; and since the highest encapsulation efficiency and bacterial release were observed at a gelatin concentration of 1.5%, this concentration was considered in mixture with alginate for encapsulation. The application of this formulation which is based on encapsulation and nanotechnology appears to be a promising technique to deliver PGPR in soil and is more effective for plants.

• KSBB Journal
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• 제22권1호
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• pp.7-15
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• 2007

진균류 추출물로부터 신규 멜라닌 분해효소의 분리 및 분해능 test는 멜라닌 분해효소의 균사체 배양 상등액으로 분비 생산됨을 분해능 테스트를 통하여 확인하였고, 이 분해 효소에 의한 멜라닌 분해반응은 pH 7에서 가장 높고, 30$^{\circ}C$에서 가장 안정함을 확인하였다. 멜라닌 분해 효소의 characterization은 2D-gel을 이용하여 멜라닌 분해효소 중 한 가지 효소의 pI값이 약 6.5이고 Molecular weight는 약 54-57 kDa임을 알 수 있었고, 정제된 멜라닌 분해 효소의 전체적인 단백질 및 유전자 서열을 분석하여 degenerate primer를 합성하였으며, RT-PCR 법을 이용하여 유전자를 확보하였다. 멜라닌 분해효소 생산을 위한 진균류의 배양방법 확립은 멜라닌 분해효소가 최대 활성을 갖는 배지 조건은 ammonium tartrate 0.4% : glucose 2% 배지에 yeast extract를 0.1%를 첨가한 배지임을 확인하였고, 진군류의 최적 배양온도는 24-26$^{\circ}C$였으며, 이 때 건조 균체 중량으로 약 6 g/L의 균체를 얻을 수 있었다. 또한 진균류 성장의 최적 pH는 약 5.5로서 이 경우 건조균체중량이 약 6.6 g/L였다. 정제공정은 최적화를 통하여 멜라닌 분해효소의 정제 순도 90% 이상의 정제공정을 확립하였다. Scale-up은 5 L fermenter를 이용한 기초 배양공정을 확립하여 500 L fermenter로의 경제성 있는 scale up에 성공하였고, 이 때 건조균체중량 약 14.5 g/L의 진균류를 얻었으며, 배지 중에 분비 생산된 melanin 분해효소의 양이 300 mg/L에 달하였다. 멜라닌 분해효소의 formulation은 멜라닌 분해효소를 효율적으로 피부로 전달시키기 위하여 50-100 nm 크기로 encapsulation을 실시하여 70 nm, 100 nm size의 nano capsule을 얻었다. 본 연구는 tyrosinase 저해제가 갖는 부작용이 없어 생체 친화적 물질에 의한 부작용 감소 효과가 기대되며, 독자적 기술에 의한 고부가 미백용 화장품 원료 확보로 새로운 기능에 의한 신규시장 창출이 가능하여 미백용 기능성 화장품 원료로서 고부가 화장품에 사용되어 수입대체효과 및 기업의 매출증대 효과가 있을 것으로 기대되어진다.

• Journal of Biosystems Engineering
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• 제42권4호
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• pp.323-329
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• 2017

Purpose: Biocompatible capsules have recently been highlighted as a novel platform for delivering various components, such as drug, food, and agriculture pesticides, to overcome the current limitations of living systems, such as those in agriculture, biology, the environment, and foods. However, few active targeting systems using biocompatible capsules and physical forces simultaneously have been developed in the agricultural engineering field. Methods: Here, we developed an active targeting delivery platform that uses biocompatible alginate capsules and controls movements by magnetic forces for agricultural and biological engineering applications. We designed and fabricated large-scale biocompatible capsules, using custom-made nozzles ejecting alginate solutions for encapsulation. Results: To develop the active target delivery platforms, we incorporated iron oxide nanoparticles in the large-scale alginate capsules. The sizes of alginate capsules were controlled by regulating the working conditions, such as concentrations of alginate solutions and iron oxide nanoparticles. Conclusions: We confirmed that the iron oxide particle-incorporated large-scale alginate capsules moved actively in response to magnetic fields, which will be a good strategy for active targeted delivery platforms for agriculture and biological engineering applications, such as for the controlled delivery of agriculture pesticides and biocontrol agents.

• Journal of Nutrition and Health
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• 제42권8호
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• pp.750-758
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• 2009

건강한 성인 남자 48명을 대상으로 각각 당근즙 섭취군과 ${\beta}$-carotene 섭취군, placebo 약 섭취군으로 나누어 8주동안 실시하였으며, 당근즙은 매일 300 mL을 주었고 정제된 항산화 비타민은 당근즙에 함유된 ${\beta}$-carotene과 같은 양을 주어 항산화 영양 상태에 대한 개선 효과를 비교하고자 하였다. 그 결과, 혈장 비타민 C, 비타민 E 및 ${\beta}$-carotene 함량은 당근즙 및 항산화 비타민 섭취 전보다 유의적으로 높게 나타났고, 그 증가 정도가 비슷하게 나타나 식품으로부터 항산화 영양소를 충분히 섭취하는 것이 흡연자의 산화적 손상과 관련된 질병을 예방하는 좋은 방법이 될 수 있을 것이라 사료된다.

• 폴리머
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• 제28권5호
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• pp.404-411
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• 2004

인계 난연제인 트리페닐 포스페이트 (TPP)와 열적, 기계적 성질이 우수하고 네트워크 구조를 형성하는 노블락 형의 에폭시 수지를 이용하여 마이크로캡슐을 제조하였다. 유용성 인계 난연제인 TPP는 고분자압출 공정 가공 시 고분자 수지에서 기화 및 방출로 인하여 난연제의 손실과 고분자 복합재의 젖음성 문제들을 야기 시킨다. 이를 해결하기 위해 TPP를 마이크로캡슐화하였다. 즉, 본 공정은 캡슐의 심물질인 TPP와 벽막 물질인 노블락 형의 에폭시 레진을 혼합된 유화제와 함께 수중유형 (O/W) 상태로 역상유화시키고 제조된 유화액을 인시츄 중합법으로 가교반응을 진행하였다. 혼합된 유화제의 비율과 양 그리고 TPP 함량에 따른 실험을 진행하였으며 마이크로캡슐의 형성 및 열적 특성의 확인을 위해 DSC와 TGA에 의해 분석하였다. 또한 캡슐 입자의 형태학적 고찰을 위해 SEM과 TEM을 이용하여 캡슐의 크기 및 모폴로지 등을 분석하였다. 혼합된 유화제의 비율이 플로닉 Fl27과 소디움 도데실벤젠 설포네이트 (SDBS)가 1:1 일 때, 그리고 유화제의 도입량이 증가할수록 캡슐의 입자가 구형이며 좀 더 균일한 입자 형태를 보였다.

• 한국응용과학기술학회지
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• 제36권2호
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• pp.592-599
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• 2019

This study is to stabilize insoluble and unstable active ingredient which is Idebenone (INCI name: hydroxydecyl ubiquinone) in a multi-lamellar vesicle (MLV) and to stabilize it in the skin care cosmetics. Idebenone is good effective raw material in the treatment of Alzheimer's disease in the medical field and a powerful antioxidant in dermatology. It is well known as a substance that inhibits the formation of melanin and cleans the skin pigment. However, it did not dissolve in any solvent and it was difficult to apply in cosmetic applications. Niosome vesicle was able to develop a nano-particle by making a multi-layer of idebenone encapsulated with a nonionic surfactant, hydrogenated lecithin and glycine soja (soybean) sterols and passing it through a high pressure microfluidizer. Idebenone niosome vesicle (INV) has been developed to have the ability to dissolve transparently in water and to promote transdermal penetration. The appearance of the INV was a yellowish liquid having specific odor, and the particle size distribution of INV was about 10~80 nm. The pH was 5~8 (mean=6.8). This capsulation with idebenone was stored in a $45^{\circ}C$ incubator for 3 months and its stability was observed and quantitatively measured by HPLC. As a result, the stability of the sample encapsulated in the niosome vesicle (97.5%) was about 66.3% higher than that of the non-capsule sample of 32.5%. Idebenone 1% INV was used for the efficacy test and clinical trial evaluation as follows. The anti-oxidative activity of INV was 38.2%, which was superior to that of 12.8% tocopherol (control). The melanin-reducing effect of B16 melanoma cells was better than INV (17.4%) and Albutin (control) (9.6%). Pro-collagen synthesis rate was 128.2% for INV and 89.3% for tocopherol (control). The skin moisturizing effect was 15.5% better than the placebo sample. The elasticity effect was 9.7% better than the placebo sample. As an application field, INV containing 1% of idebenone is expected to be able to develop various functional cosmetic formulations such as skin toner, ampoule essence, cream, eye cream and sunblock cream. In addition, it is expected that this encapsulated material will be widely applicable to emulsifying agents for skin use in the pharmaceutical industry as well as the cosmetics industry.