• Korean journal of applied entomology
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• v.53 no.1
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• pp.85-91
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• 2014

In this study, we analyzed the phylogenetic characterization of root-knot nematodes (Meloidogyne spp.) in soils from fruits and vegetables greenhouses in Korea. Soil samples were collected from 12 greenhouse fields in which tomato, cucumber, watermelon, and Oriental melon were being cultivated. Meloidogyne spp. were detected in all the soil samples at an average number of $72{\pm}6$ nematodes/300 g of soil to $2,898{\pm}468$ nematodes/300 g of soil. Phylogenetic analysis using polymerase chain reaction-restriction fragment length polymorphism was attempted for the second-stage juveniles (J2) of Meloidogyne spp. collected from the greenhouse soils. Twelve Meloidogyne spp. from the greenhouse soils were classified into two groups by using HinfI digestion of mitochondrial DNA, resulting in 900, 410, 290, and 170 bp fragments (group A) and 900, 700, and 170 bp fragments (group B). Phylogenetic analysis based on mitochondrial DNA sequences (1,483-1,521 bp) showed that nine group A isolates were identified as Meloidogyne incognita (99.73-99.93%) and three group B isolates showed 99.54-99.73% similarity to Meloidogyne arenaria.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.2
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• pp.183-189
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• 2018

Blue light is the highest energy wavelengths in the visible light region and induces skin aging and active oxygen. Studies on harmful mechanism of skin are under way. Research on blue light blocking materials in cosmetics and formulation studies are in the early stage, and the test methods related to blue light blocking measurement are not established. The blue light blocking efficacy was established by referring to the test method of the sunscreen in vitro test(COLIPA guideline, ISO 24443, FDA Final Rule on Sunscreen Testing and Labeling). The blue light blocking effect was evaluated for 17 kinds out of 27 kinds of sunscreen raw materials suggested in KFDA guideline. The Effect was 14.97% for zinc oxide and 16.32% for bishexyloxyphenol methoxyphenyl triazine, 35.47% for methylene bis-benzotriazolyltetramethylbutylphenol, and 65.96% for titanium dioxide. In addition, the effect of micro-titanium dioxide was twice as high as that of the nano-titanium dioxide. The results suggested that the light blocking effect test method can be used to search for blue light blocking materials and study cosmetic formulations.

• Microbiology and Biotechnology Letters
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• v.40 no.3
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• pp.274-277
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• 2012

Cytokinesis is the final stage of cell division, dividing one mother cell into two daughter cells. For the cutting of a plasma membrane during bacterial cytokinesis, a tubulin homolog FtsZ protein is recruited from the cytoplasm to the division site. FtsZ protein polymerizes in a GTP-dependent manner and its N-terminal domain has a GTPase activity. In this study, we have begun to characterize FtsZ from Staphylococcus aureus (SA). Full-length SA FtsZ was cloned into pRSFDuet-1 vector and the clone was transformed into a BL21 (DE3) star cell. The recombinant SA FtsZ protein was purified using Ni-NTA affinity chromatography and dialysis. Using a spectrofluorometer, we showed that SA FtsZ undergoes a GTP-dependant polymerization in vitro. The polymer of the SA FtsZ protein disappeared after a few minutes, suggesting that the polymer is degraded as the GTP is consumed. This assay system may well be applied for inhibitor screening targeting S. aureus FtsZ.

• Clean Technology
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• v.28 no.2
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• pp.123-130
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• 2022

Nanoparticles are used in various fields such as chemistry, medicine, the environment, and information and communication. With the increasing use of engineered nanomaterials, exposure to nanoparticles is expected to increase in the workplace and the environmental media. However, while nanotechnology industries are expanding, research on the exposure assessment of nanomaterials to humans and the environment is only at a beginning stage. Especially, if nanoparticles with a size of 100 nm or less that are contained in nano-products are released unintentionally, they may pose potential risks to the human body through breathing or skin exposure. Therefore, in this work, the possibility of potential exposure of nanoparticles moving from the laboratory to the office was confirmed, and nanoparticle safety guidelines are proposed. A nano-collector was used to detect nanoparticles in the atmosphere, and through use of a scanning mobility particle sizer it was found that nanoparticle concentrations in the laboratory and the office tended to be similar. On the assumption that nanoparticles attached to a lab-coat move out of the laboratory, a lab-coat to which nanocarbon black was attached was shaken and the concentration of the remaining particles on the lab-coat determined. The results confirmed that sufficient amounts of nanoparticles attached to the lab-coat could move from the laboratory to the office along the path of a researcher; thus, safety guidelines for the handling of lab-coat nanoparticles are required.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.4
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• pp.705-713
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• 2016

For the soft ground construction, the factors not considered in the design stage occurs in the construction stage so that they cause the increase of the construction cost due to the structural stability and the design change. The subject of the study is the construction section of the industrial complex access road made in the Ochang region of Chungcheongbuk-do. The study is concerned with selecting the soft ground handling method such as the replacement method using rock debris and the surcharge reflecting the service load as the soft ground handling measure and analyzing the effect of reducing the construction cost with the stability of structures and the reduction of the construction period. The soft ground in the study section consists of sandy and cohesive soil and is 2.4m to 5.5m deep. It is distributed unevenly between the 1.5m to 5.9m stratums under the ground surface. Settlement is not serious, but the future uneven settlement and difference are expected so that the future settlement behavior is estimated by analyzing the site measurement results after the soft ground treatment. Moreover, in consideration of the regional characteristics and economic efficiency, soil with good quality is replaced with rock debris as the replacement material so that 29% of the construction cost is reduced due to the increase of stability and the reduction of duration. If the estimation of the dispersion of the pore water pressure within the dam body and the change of the underground water level and the relation of the actually measured soft ground with consolidation is studied further on the basis of the study, it is expected that the behavior of the soft ground will be correctly estimated in various site conditions.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.228.2-228.2
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• 2015

We have developed a TOF-MEIS system using 70~100 keV He+. A TOF-MEIS system was designed and constructed to minimize the ion beam damage effect by utilizing a pulsed ion beam with a pulse width < 1 ns and a TOF delay-line-detector with an 120 mm diameter and a time resolution of 180 ps. The TOF-MEIS is an useful tool for interfacial analysis of the composition and structure of nano and bio systems. Our recent applications are reported. We investigated the effect with Polyaspartic Acid (pAsp) and Osteocalcin on the initial bone growth of calcium hydroxyl appatite on a carboxyl terminated surface. When pAsp is not added to the self-assembled monolayers of Ca 2mM with Phosphate 1.2 mM, the growth procedure of calcium hydroxyl appatite cannot be monitored due to its rapid growth. When pAsp is added to the SAMs, the initial grow stage of the Ca-P can be monitored so that the chemical composition and their nucleus size can be analyzed. Firstly discovered the existence of 1-nm-sized abnormal calcium-rich clusters (Ca/P ~ 3) comprised of three calcium ions and one phosphate ion. First-principles studies demonstrated that the clusters can be stabilized through the passivation of the non-collagenous-protein mimicking carboxyl-ligands, and it progressively changes their compositional ratio toward that of a bulk phase (Ca/P~1.67) with a concurrent increase in their size to ~2 nm. Moreover, we found that the stoichiometry of the clusters and their growth behavior can be directed by the surrounding proteins, such as osteocalcin.

• Korean journal of applied entomology
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• v.14 no.1 s.22
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• pp.29-33
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• 1975

The experiment It·as conducted to find out the distribution of fenitrothion in rice plants at the preheading stage and its residue in harvested grains. The results obtained were as follows; 1. Distribution of fenitrothion in rice plants were measured 15.32 ppm attached to the leaf surface immediately after spray and 6.73 ppm was penetrated into tissues after 24 hours. 2. Residual amounts of fenitrothion in rice grains was 0.04 ppm at chaff and 0.01 ppm at unpolished rice, respectively.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.5
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• pp.45-49
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• 2010

The automotive industry has shown a growing interest in tube hydroforming during the past years. The advantages of hydroforming (less thinning, a more efficient manufacturing process, etc.) can, for instance, be combined with the high strength of extra high strength steels, which are usually less formable, to produce structural automotive components which exhibit lower weight and improved service performance. Design and production of tubular components require knowledge about tube material and forming behavior during hydroforming and how the hydroforming operation itself should be controlled. These issues are studied analytically in the present paper. In this study, the whole process of rear sub-frame parts development by tube hydroforming using AA6061 material is presented. At the part design stage, it requires feasibility study and process design aided by CAE (Computer Aided Engineering) to confirm hydroformability in details. Effects of parameters such as internal pressure, axial feeding and geometry shape in automotive rear sub-frame by hydroforming process were carefully investigated. Overall possibility of hydroformable rear sub-frame parts could be examined by cross sectional analyses. Moreover, it is essential to ensure the formability of tube material on every forming step such as pre-bending and hydroforming. In addition, all the components of prototyping tool are designed and interference with press is examined from the point of geometry and thinning.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.3
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• pp.1051-1057
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• 2011

By the recently developed GMP(Glass Molding Press) process, aspheric glass lenses are widely used in many optical applications such as digital cameras, optical data storages and electrical devices etc. The GMP process can economically produce complex shaped glass lenses with high precision and good repeatability. This study deals the optimization of molding conditions for aspheric glass lenses in progressive GMP process through Design Of Experiment(Taguchi method). Tree main factors for molding conditions were selected based on pressure, temperature and cooling time at 1st cooling stage. From the analysis of experiments which were preformed with 3-cavity glass mold, it was revealed that the cooling time was the most sensitive parameter for form accuracy(PV) in progressive GMP process.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.10
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• pp.1521-1527
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• 2010

Since inkjet printing is being employed in production lines of electronics and display industries, the tack time for inspection of jetting failure has become very important because the throughput of the inkjet printing system can be extended to the maximum limit by adopting a shorter jetting inspection time. The most popular method for inspecting jetting failure involves the use of a linear stage, a high magnification lens, and a charge coupled devicecamera. However, this conventional approach requires approximately 60 s to complete the jetting inspection and might not be suitable for a high-speed reciprocating jetting inspection in endurance tests due to the unwanted mechanical vibration. In this study, a novel concept of an inkjet monitoring module is introduced, which has an overall inspection time of 18 s. For the shorter tack time of jetting inspection, the parameters affecting the tack time are discussed in this paper.