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Attenuation Effects of Herbal Mixture Extract on Stress and Sleep Disturbance in Rats (구속 스트레스 및 수면장애를 유도한 흰쥐에서 허브 복합추출물에 의한 개선 효과)

  • Jung, An Na;Lee, Bo Kyung;Lee, Doo Yi;Lee, Ji In;Jung, Yi-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.3
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    • pp.313-320
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    • 2016
  • In the present study, we examined whether or not an herbal mixture extract (HME) has attenuation effects on immobilization stress and sleep disturbance in rats. Immobilization stress was induced by restricting movement using a constraint box for 2 h, and sleep disturbance was induced by exposure to 300 lx of constant light for 24 h and injection of caffeine (1 mg/kg, i.p.). Rats were orally treated with distilled water (vehicle) or HME for 3 weeks at different doses of 10, 30, and 100 mg/kg/d (HME I, II, and III, respectively). In the immobilization model, HME III treatment significantly decreased adrenal gland weight, whereas HME II and III treatment reduced plasma levels of cortisol. HME II and III also reduced the level of IL-6. In the sleep disturbance model, HME II and III meaningfully reduced the plasma level of cortisol, and the increased plasma level of melatonin. HME III significantly increased body weight. HME reduced immobilization stress and ameliorated disturbance in rats. These findings suggest that HME may have beneficial potential for attenuation of sleep disturbance and stress.

Prevalence of Salmonella Species Isolated from Old Hen Delivery Trucks in Korea and Application of Disinfectant for the Reduction of Salmonella Contamination (국내 노계 운반 전용 차량에서의 살모넬라 오염율 및 차량소독에 따른 오염도 감소 효과)

  • Na, Deok-Hwan;Hong, Young-Ho;Yoon, Mi-Young;Park, Eun-Jin;Lim, Tae-Hyun;Jang, Jun-Hyuk;Kim, Byoung-Youn;Lee, Dong-Hun;Song, Chang-Seon
    • Korean Journal of Poultry Science
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    • v.40 no.1
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    • pp.11-16
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    • 2013
  • Salmonella is closely related with human health of modern society which has concern increased in livestock goods consumption as well as give economic damage throughout the chicken industry such as farm, hatchery, slaughter house and processing plant. From 2007 to 2011, this study investigated Salmonella prevalence from 200 old hen delivery trucks which deliver old egg-laying hens and broiler breeders in Korea. The prevalence of Salmonella species was 38.0% in old hen delivery truck. Serogroup C1 was the most frequently detected serogroup of Salmonella, followed by the serogroups D1, C2 and B. A total of 25 serotypes were identified and Salmonella infantis was the most frequently isolated serotype. In addition, we applied disinfectant to old hen delivery truck for the reduction of Salmonella contamination. The disinfectant consists of formaldehyde, glutaladehyde and quaternary ammonium compound was applied to the trucks. Salmonella isolation rate was significantly decreased after disinfection from 38.0% to 7.5%. Disinfectant could not effectively reduce Salmonella contamination at a dilution of 1:200 which is recommended by manufacturer, but Salmonella isolation rate was significantly decreased at a dilution of 1:50. Since old hen delivery truck could be a potential vector to carry Salmonella into farms and abattoirs, chicken delivery truck should be disinfected thoroughly and sufficiently to control contamination of farms and abattoirs.

Effect of Phosphate Bio fertilizer Produced by Enterobacter intermedium on Rhizosphere Soil Properties and Lettuce Growth (Enterobacter intermedium으로 제조된 인산생물비료가 토양 특성 및 상추의 생육에 미치는 영향)

  • Park, Bum-Ki;Na, Jung-Heang;Hwang-Bo, Hoon;Lee, In-Jung;Kim, Kil-Yong;Kim, Yong-Woong
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.1
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    • pp.15-24
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    • 2005
  • Enterobacter intermedium oxidizes glucose to gluconic acid and sequentially converts gluconic acid to 2-ketogluconic acid (2-KGA) under aerobic condition. Shaking incubation of E. intermedium in a broth medium containing 22.5 g glucose, 8.2 g gluconic acid and 40 g rock phosphate per liter resulted in $1028mg\;L^{-1}$ soluble phosphate. The culture broth was used as phosphate bio-fertilizer (PBF) in this experiment. To evaluate PBF produced by E. intermedium on lettuce growth, five treatments (PBF1/3, PBF2/3, PBF3/3, BP, and MF) were used. In MF and BP treatments, $P_2O_5$ 5.9 kg of mineral fertilizer per 10a was added, while in PBF1/3, PBF2/3, and PBF3/3 treatments, culture broth containing one third, two third, and same amount of soluble $P_2O_5$ 5.9 kg per 10a was applied, respectively. At 20, 35, and 50 days after transplanting of lettuce, plant growth components, biomass, enzyme activities and soil chemical properties were analyzed. Dehydrogenase activity and available phosphate concentration of rhizosphere in phosphate bio fertilizer treatments (PBF1/3, PBF2/3, PBF3/3) were generally higher compared to MF and BP treatments. Soil biomass in PBF3/3 treatment was significantly higher than MF and BP treatments at early growth stage. However, there was no significant difference among all treatments with time. Plant fresh weights in PBF1/3, PBF2/3, and MF treatments were better than those in BP and PBF3/3 treatments. However, in PBF2/3 treatment the highest fresh weight was discovered where alkaline phosphatase activity was generally higher than other treatments at 35 and 50 days. Enhancement of lettuce growth at 35 and 50 days in PBF2/3 treatment was associated with greater phosphate uptake in lettuce tissue. As regarding all results, PBF showed better lettuce growth compared to mineral phosphate fertilizer where PBF2/3 treatment resulted in increase of lettuce fresh weight by 23% and phosphate uptake by 50%.

Geochemistry and Genesis of Hydrothermal Cu Deposits in the Gyeongsang Basin, Korea : Hwacheon-ri Mineralized Area (경상분지내 열수동광상의 지화학 및 성인연구 : 화천리지역 광화대)

  • So, Chil-Sup;Choi, Sang-Hoon;Yun, Seong-Taek
    • Economic and Environmental Geology
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    • v.28 no.4
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    • pp.337-350
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    • 1995
  • The Hwacheon-ri mineralized area is located within the Cretaceous Gyeongsang Basin of the Korean peninsula. The mineralized area includes the Hwacheon, Daeweon, Kuryong and Cheongryong mines. Each of these mines occurs along copper-bearing hydrothermal quartz veins that crosscut late Cretaceous volcanic rocks, although some disseminated ores in host rocks also exist locally. Mineralization can be separated into three distinct stages (I, II, and III) which developed along preexisting fracture zones. Stage I is ore-bearing, whereas stages II and III are barren. The main phase of ore mineralization, stage I, can be classified into three substages (Ia, Ib and Ic) based on ore mineral assemblages and textures. Substage Ia is characterized by pyrite-arsenopyrite-molybdenite-pyrrhotite assemblage and is most common at the Hwacheon deposit. Substage Ib is represented by main precipitation of Cu, Zn, and Pb minerals. Substage Ic is characteristic of hematite occurrence and is shown only at the Kuryong and Cheongryong deposits. Some differences in the ore mineralization at each mine in the area suggest that the evolution of hydrothermal fluids in the area varied in space (both vertically and horizontally) with respect to igneous rocks relating the ore mineralization. Fluid inclusion data show that stage I ore mineralization mainly occurred at temperatures between ${\approx}350^{\circ}$ and ${\approx}200^{\circ}C$ from fluids with salinities between 9.2 and 0.5 wt.% eq. NaCl. In the waning period of substage Ia, the high temperature and salinity fluid gave way to progressively cooler, more dilute fluids of later substage Ib and Ic (down to $200^{\circ}C$, 0 wt.% NaCl). There is a systematic decrease in the calculated ${\delta}^{18}O_{H2O}$ values with paragenetic time in the Hwacheon-ri hydrothermal system from values of ${\approx}2.7$‰ for substage Ia, through ${\approx}-2.8$‰ for substage Ib, to ${\approx}-9.9$‰ for substage Ic. The ${\delta}D$ values of fluid inclusion water also decrease with decreasing temperature (except for the Daeweon deposit) from -62‰ (substage Ia) to -80‰ (substage Ic and stage III). These trends are interpreted to indicate the progressive cooler, more oxidizing unexchanged meteoric water inundation of an initial hydrothermal system which is composed of highly exchanged meteoric water. Equilibrium thermodynamic interpretation of the mineral assemblages with the variation in amounts of chalcopyrite through the paragenetic time, and the evolution of the Hwacheon-ri hydrothermal fluids indicate that the solubility of copper chloride complexes in the hydrothermal system was mainly controlled by the variation of temperature and $fo_2$ conditions.

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Effects of Organic Salts Fortified with Seaweed Components on Blood Pressure, Serum Minerals, and Hematochemicals in Spontaneously Hypertensive and Normotensive Rats (해조성분 강화염이 본태성 고혈압쥐와 정상혈압쥐의 혈압, 혈청 중 미네랄 함량 및 생화학적 특성에 미치는 영향)

  • Kim, Young-Myoung;Byun, Jee-Young;Han, Chan-Kyu;Sung, Ki-Seung;Namgung, Bae
    • Korean Journal of Food Science and Technology
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    • v.41 no.2
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    • pp.196-202
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    • 2009
  • The effects of the salts fortified with seaweed functional components on blood pressure, serum minerals, and hematochemicals in spontaneously hypertensive rats (SHR/NCrj) and normotensive rats (WKY/NCrj) were investigated. SHR and WKY rats were assigned to four groups, with 8 and 6 rats in each group: laver salt (A), fucoidan+laver high salt (B), fucoidan + laver low salt (C) and refined salt as a control (D). The final blood pressure (BP) of SHR and WKY species in contrast with reference BP were low in groups A and C as compared with control group. In terms of serum mineral content, $Na^+$ levels were similar in SHR and WKY, but $K^+$ levels were higher in the group B in SHR. Serum triglyceride levels were lower in groups A and C, but the levels of HDL- and LDL- cholesterol were significantly higher and lower in group A than those of controls, respectively (p<0.05). These results demonstrated that the salts fortified with laver might suppress blood pressure in rats, and also may improve mineral and lipid metabolism.

Data Mining Approaches for DDoS Attack Detection (분산 서비스거부 공격 탐지를 위한 데이터 마이닝 기법)

  • Kim, Mi-Hui;Na, Hyun-Jung;Chae, Ki-Joon;Bang, Hyo-Chan;Na, Jung-Chan
    • Journal of KIISE:Information Networking
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    • v.32 no.3
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    • pp.279-290
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    • 2005
  • Recently, as the serious damage caused by DDoS attacks increases, the rapid detection and the proper response mechanisms are urgent. However, existing security mechanisms do not effectively defend against these attacks, or the defense capability of some mechanisms is only limited to specific DDoS attacks. In this paper, we propose a detection architecture against DDoS attack using data mining technology that can classify the latest types of DDoS attack, and can detect the modification of existing attacks as well as the novel attacks. This architecture consists of a Misuse Detection Module modeling to classify the existing attacks, and an Anomaly Detection Module modeling to detect the novel attacks. And it utilizes the off-line generated models in order to detect the DDoS attack using the real-time traffic. We gathered the NetFlow data generated at an access router of our network in order to model the real network traffic and test it. The NetFlow provides the useful flow-based statistical information without tremendous preprocessing. Also, we mounted the well-known DDoS attack tools to gather the attack traffic. And then, our experimental results show that our approach can provide the outstanding performance against existing attacks, and provide the possibility of detection against the novel attack.

Changes in Mineral Uptake and Hormone Concentrations in Rice Plants Treated with Silicon, Nitrogen and Calcium Independently or in Combination (규소, 질소, 칼슘 단독 및 혼합처리가 벼 식물체 내 무기성분 흡수 및 식물호르몬 함량 변화에 미치는 영향)

  • Jang, Soo-Won;Kim, Yoon-Ha;Na, Chae-In;Lee, In-Jung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.4
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    • pp.293-303
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    • 2017
  • To elucidate the physiological responses of rice plants to the essential mineral silicon (Si), we assessed the effects of treatments with Si, nitrogen ($NH_4NO_3$; ammonium nitrate), and calcium ($CaCl_2$; calcium chloride), independently or in combination on mineral uptake rates and levels of the hormones abscisic acid (ABA), gibberellin ($GA_1$) and jasmonic acid (JA). We found that nitrogen and calcium uptake was inhibited by Si application. However, solo application of nitrogen or calcium did not affect Si uptake. Compared to the untreated plants, the application of Si, $NH_4NO_3$ or $CaCl_2$ increased the endogenous hormone levels in treated plants. In particular, the concentrations of $GA_1$ and JA increased significantly after the application of Si or $NH_4NO_3$. The level of $GA_1$ observed after a treatment (solo or combine) with Si, and $NH_4NO_3$ was higher than that of the control. By contrast, independent application of $CaCl_2$ or a combined treatment with Si and $CaCl_2$ did not alter $GA_1$ levels. The highest level of $GA_1$ was present in plants given a combination treatment of Si and $NH_4NO_3$. This effect was observed at all time points (6 h, 12 h and 24 h). Endogenous JA contents were higher in all treatments than the control. In particular, a combination treatment with Si and $NH_4NO_3$ significantly increased the JA levels in plants compared to other treatments at all time points. A small increase in JA levels was observed after 6 h in plants given the $CaCl_2$ treatment. However, JA levels did not differ between plants given a $CaCl_2$ treatment and controls after 12 h or 24 h of exposure. We conclude that treatment with $CaCl_2$ alone does not affect endogenous JA levels in the short term. Endogenous ABA contents did not show any differences among the various treatments.

Bonding efficacy of cured or uncured dentin adhesives in indirect resin (간접 레진수복시 상아질 접착제의 중합 여부에 따른 결합 효능)

  • Jang, Ji-Hyun;Lee, Bin-Na;Chang, Hoon-Sang;Hwang, Yun-Chan;Oh, Won-Mann;Hwang, In-Nam
    • Restorative Dentistry and Endodontics
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    • v.36 no.6
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    • pp.490-497
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    • 2011
  • Objectives: This study examined the effect of the uncured dentin adhesives on the bond interface between the resin inlay and dentin. Materials and Methods: Dentin surface was exposed in 24 extracted human molars and the teeth were assigned to indirect and direct resin restoration group. For indirect resin groups, exposed dentin surfaces were temporized with provisional resin. The provisional restoration was removed after 1 wk and the teeth were divided further into 4 groups which used dentin adhesives (OptiBond FL, Kerr; One-Step, Bisco) with or without light-curing, respectively (Group OB-C, OB-NC, OS-C and OS-NC). Pre-fabricated resin blocks were cemented on the entire surfaces with resin cement. For the direct resin restoration groups, the dentin surfaces were treated with dentin adhesives (Group OB-D and OS-D), followed by restoring composite resin. After 24 hr, the teeth were assigned to microtensile bond strength (${\mu}TBS$) and confocal laser scanning microscopy (CLSM), respectively. Results: The indirect resin restoration groups showed a lower ${\mu}TBS$ than the direct resin restoration groups. The ${\mu}TBS$ values of the light cured dentin adhesive groups were higher than those of the uncured dentin adhesive groups (p < 0.05). CLSM analysis of the light cured dentin adhesive groups revealed definite and homogenous hybrid layers. However, the uncured dentin adhesive groups showed uncertain or even no hybrid layer. Conclusions: Light-curing of the dentin adhesive prior to the application of the cementing material in luting a resin inlay to dentin resulted in definite, homogenous hybrid layer formation, which may improve the bond strength.

Effects of Dietary Levels of Corn Distiller's Dried Grains with Solubles and Phytase on Performance and Nutrient Utilization of Broilers (옥수수 주정박의 수준과 Phytase의 첨가 유무가 육계의 생산성과 영양소 이용률에 미치는 영향)

  • Hong, E.C.;Na, S.H.;Yu, D.C.;Kim, H.K.;Park, M.N.;Jung, K.C.;Choo, H.J.;Park, H.D.;Chung, W.T.;HwangBo, J.
    • Korean Journal of Poultry Science
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    • v.36 no.3
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    • pp.247-255
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    • 2009
  • This work was conducted to investigate the effects of the addition level of Corn distiller's dried grains with solubles (CDDGS) and phytase in broiler diets on the broiler performance and the ileal amino acid digestibility. Three eighty four Ross broiler (one-day-old) were assigned randomly to 8 treatments groups (12 birds/pen, 4 pens/treatment) as $4{\times}2$ complex factors of CDDGS 4 treatments (0, 5, 10 and 15%) and phytase 2 treatments (0, 500 FTU/kg), and fed experimental diets for 6 weeks (starter, 0~2 wk; grower, 2~4 wk; finisher, 4~6 wk). After the feeding trial was finished, 12 broilers (6 weeks) with similar weights were selected from all treatments to investigate the digestibility of nutrients and amino acids for the first week. The experimental diets contained similar ME and CP of all treatments. Body weight gains and feed intakes were high at phytase treatments (P>0.05), but there was no difference in feed intake of the chicks. The amino acid digestibilities of diets were improved for iso-leucine, lysine, threonine, valine, aspartic acid, proline, serine and tyrosine (P<0.05). Finally, this work showed that growth performance and amino acid digestibility were improved by the addition of CDDGS and phytase in broiler diets.

Effects of Feeding Induced Molting on the Performance, Egg Quality, and Visceral Organs in Laying Hens (산란계에서 급이환우가 산란계의 생산성과 계란의 품질 및 주요 장기에 미치는 영향)

  • Hong, E.C.;Na, J.C.;You, D.C.;Jang, B.G.;Kim, H.K.;Choi, Y.H.;Park, H.D.;HwangBo, J.
    • Korean Journal of Poultry Science
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    • v.34 no.3
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    • pp.197-205
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    • 2007
  • This study was conducted to compare the effect of feeding molting and fasting molting on the performance, egg quality, and visceral organs in laying hens for animal welfare. Eighty one 62-wk-old White Leghorn hens that egg production was over 80% and average weight was $1.6{\pm}0.3\;kg$ were used in this study. Treatments were control (non-molt treatment), feeding molt treatment, and fasting molt treatment. The three treatments were administered to three replicate group of nine hens wherein each group. All treatment groups were fed the basal diet (CP 15%, ME 2,700 kal/kg) for two weeks as the adaptation period. Induced molt diets contains low CP (6.7%) and low energy (2,200 kal/kg). Test periods were 14 days for feeding molting and 10 days for fasting molting. Egg production decreased to be 0% at 10 days of feeding molting treatment, but at 2 or 3 days of fasting molting treatment. Egg production restarted after 19 days ending molt at feeding molting treatment, while after 24 days at fasting molting treatment. On the egg quality was improved at molting treatments (p<0.05) except egg yolk. Egg shell tissue was crowded at molting treatment to compare to control. Liver weights, heart weight, and oviduct weight of laying hens decreased at molting treatments (p<0.05). Finally, feeding molting might could be replaced fasting molting on the welfare and further studies were needed about molting program.