Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Attenuation Effects of Herbal Mixture Extract on Stress and Sleep Disturbance in Rats

구속 스트레스 및 수면장애를 유도한 흰쥐에서 허브 복합추출물에 의한 개선 효과

  • Jung, An Na (Department of Pathophysiology, College of Pharmacy, Ajou University) ;
  • Lee, Bo Kyung (Department of Pathophysiology, College of Pharmacy, Ajou University) ;
  • Lee, Doo Yi (Farm Herbnara, Pyeongchang) ;
  • Lee, Ji In (Farm Herbnara, Pyeongchang) ;
  • Jung, Yi-Sook (Department of Pathophysiology, College of Pharmacy, Ajou University)
  • 정안나 (아주대학교 약학대학 병태생리학교실) ;
  • 이보경 (아주대학교 약학대학 병태생리학교실) ;
  • 이두이 (평창 허브나라 농원) ;
  • 이지인 (평창 허브나라 농원) ;
  • 정이숙 (아주대학교 약학대학 병태생리학교실)
  • Received : 2015.10.17
  • Accepted : 2016.01.04
  • Published : 2016.03.31
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Abstract

In the present study, we examined whether or not an herbal mixture extract (HME) has attenuation effects on immobilization stress and sleep disturbance in rats. Immobilization stress was induced by restricting movement using a constraint box for 2 h, and sleep disturbance was induced by exposure to 300 lx of constant light for 24 h and injection of caffeine (1 mg/kg, i.p.). Rats were orally treated with distilled water (vehicle) or HME for 3 weeks at different doses of 10, 30, and 100 mg/kg/d (HME I, II, and III, respectively). In the immobilization model, HME III treatment significantly decreased adrenal gland weight, whereas HME II and III treatment reduced plasma levels of cortisol. HME II and III also reduced the level of IL-6. In the sleep disturbance model, HME II and III meaningfully reduced the plasma level of cortisol, and the increased plasma level of melatonin. HME III significantly increased body weight. HME reduced immobilization stress and ameliorated disturbance in rats. These findings suggest that HME may have beneficial potential for attenuation of sleep disturbance and stress.

본 연구에서는 라벤더, 캐모마일, 레몬버베나, 타임, 바질의 복합 열수 추출물이 수면을 촉진하고 스트레스를 완화하는지 확인하기 위하여 농도에 따라 동물모델에 미치는 효과를 관찰하였다. 허브 복합추출물(HME)을 10, 30, 100 mg/kg의 농도로 3주간 구속 스트레스 모델과 수면장애 모델에 각각 경구투여 하였고, 실험 결과 구속 스트레스를 유도한 동물모델에서 HMEII군(30 mg/kg)과 HMEIII군(100 mg/kg)은 혈장 내 코티졸과 IL-6 함량을 감소시켰으며, HMEIII군은 체중 대비 부신 무게도 유의적으로 회복시킨 것을 확인하였다. 수면장애 모델에서는 HMEIII군이 대조군에 비해 체중이 증가하였고, HME II, III군은 혈장 내 멜라토닌 수치를 높였으며 코티졸 수치를 감소시켰다. 이러한 결과를 통해 본 실험에서 사용한 허브 복합추출물이 농도에 따라 스트레스 완화와 수면장애 개선에 효과가 있음을 확인하였다.

Keywords

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