• Title/Summary/Keyword: NaCl Stress

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Effect of Glucose on Listeria monocytogenes Survival under Sequential Sublethal Stresses of Gamma Irradiation and NaCl

  • Yoon, Yo-Han;Kim, Gyeong-Yeol;Nam, Min-Ji;Shim, Won-Bo;Seo, Eun-Kyoung;Kim, Jae-Hun;Lee, Ju-Woon;Byun, Myung-Woo;Chung, Duck-Hwa
    • Food Science and Biotechnology
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    • v.18 no.1
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    • pp.162-166
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    • 2009
  • This study evaluated glucose effect on Listeria monocytogenes survival under gamma irradiation and NaCl stress. L. monocytogenes in phosphate buffered saline (PBS) plus glucose (0-4%) was treated with gamma irradiation (0-0.5 kGy), and the samples were then exposed to NaCl (0-9%) in tryptic soy agar plus 0.6% yeast extract. $D_{10}$ and $t_{3D}$ values were determined, and a model for prediction of $D_{10}$ values was developed. Cell counts of L. monocytogenes reduced as irradiation dose increased, and L. monocytogenes in PBS (no glucose) was more sensitive to irradiation and NaCl compared to those in PBS (2 or 4% glucose). $D_{10}$ values were 0.07-0.1, 0.12-0.16, and 0.13-0.15 kGy for 0, 2, and 4% glucose, respectively. The $t_{3D}$ values were 0.22-0.3 (0% glucose), 0.35-0.48 (2% glucose), and 0.40-0.44 (4% glucose). A model performance was acceptable. These results indicate that glucose in foods would increase the resistance of L. monocytogenes to gamma irradiation and NaCl stress.

Response of Growth and photosynthesis to NaCl stress in Soybean(Glysine max L.) Seedlings (NaCl stress에 의한 몇가지 콩 품종들의 생육과 광합성 반응)

  • Cho, Jin-Woong;Kim, Choong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.19 no.2
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    • pp.166-170
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    • 2000
  • This study was conducted in to elucidate the changes of growth characteristics and photosynthesis in three soybean (Glycine max L. cv. Danwonkong, Hwangkeumkong and Kwangankong) 30 day old seedlings to 100mM NaCl concentration containing 1/2 Hoagland's nutrient solution in sand culture. The main stem height and number of main stem node were decreased. thus, leaf area and dry matter were decreased with 100mM NaCl. Growth reduction was less little in Hwangkeumkong than other cultivars. The stem growth rate was affected less than other parts as root or leaf, by NaCl treatment. The specific leaf area (SLA), shoot : root ratio and leaf : root ratio decreased with NaCl solution except for those of Hwangkeumkong. There is no general tendency in leaf thickness by leaf position of three cultivars. The chlorophyll content (SPAD) of the primary and 2nd leaf slightly decreased under NaCl solution but rapidly increased in non-NaCl solution at 15 days after treatments. The photosynthesis, stomatal conductance and transpiration of 2nd leaf positions reduced by NaCl and there were a sigificant correlation between photosynthesis and stomatal conductance or transpiration.

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Effect of Mulberry Charcoal Treatment on the Growth and Physiological Properties of Kentucky Bluegrass Grown under Salt Stress Condition (뽕나무 숯 처리가 Kentucky Bluegrass의 염해에 따른 생육과 생리적 반응에 미치는 영향)

  • Bae, Euu-Ji;Jeong, Sung-Woo;Park, Nam-Chang;Lee, Kwang-Su;Shin, Hyun-Cheol;Huh, Moo-Ryong
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.112-117
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    • 2011
  • This research was conducted to determine the effect of the pre-treatment of mulberry charcoal on the salt tolerance response in Kentucky bluegrass 'Perfection'. As this results, 400 mM NaCl treatment reduced turf growth such a fresh and dry weight, and induced physiological damages like decreased chlorophyll content and increased electrolyte leakage. The pre-treatment of mulberry charcoal, however, mitigated those growth and physiological responses associated with NaCl stress, regardless of the concentrations of charcoal solution. Furthermore, it was observed that higher K ion concentration by mulberry charcoal pre-treatment reduced Na ion in shoot. This research suggests to be a possibility of planting turf grasses in reclaimed land or higher salt accumulated area using a mulberry charcoal treatment.

The effects of 3.5% NaCl solution on the corrosion fatigue crack propagation characteristics of SS41 steel (SS41강의 부식피로 균열 전파특성에 미치는 3.5% NaCl수용액의 영향)

  • 오세욱;김재철;최영수
    • Journal of Ocean Engineering and Technology
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    • v.1 no.1
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    • pp.111-119
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    • 1987
  • The corrosion fatigue crack propagation characteristics of SS41 steel in 3.5% NaCl solution have been evaluated for loading frequencies of 1Hz and 0.2Hz. A sine wave loading profile was used for fatigue testing. Each test was carried out at a constant stress ratio, R(0.1). The main results are summarized as follows; 1. Fatigue crack propagation rate was higher in 3.5% NaCl solution than in air, higher in the base metal than in the weld metal, and higher at f =0.2Hz than at f =1Hz. 2. The crack closure level of the base metal was not influenced by cyclic frequencies, but that of the weld metal was much influenced. 3. When the crack closure effect was eliminated in the evaluation of crack propagation characteristics by using $\Delta K_{eff}$, the envirommental influence was distinctly observed. At the base metal, crack propagation rate was enhanced by the hydrogen embrittlement, and the weld metal was reduced by the crac closure. 4. There was clearly observed hydrogen embrittlement and severely corroded aspect at fracture surface of lower frequency than that of higher frequency, and at that of base metal than that of the weld metal.

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Effect of Salinity Stress on Dry Matter Yield and Oxalate Content in Napiergrass (Pennisetum purpureum Schumach)

  • Rahman, M.M.;Ishii, Y.;Niimi, M.;Kawamura, O.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.11
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    • pp.1599-1603
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    • 2008
  • Sodium is involved in elevation of oxalate content in some plant species and this element is abundant in saline soils. Oxalate causes precipitation of insoluble calcium oxalate in the rumen and kidneys. The intention of this study was to evaluate the effect of soil salinity stress on dry matter yield and oxalate content in pot-grown napiergrass (Pennisetum purpureum Schumach). Plants were cut three times at 56, 118 and 179 d after transplanting to the pots. Five salinity treatments were used containing various concentrations of NaCl solution as follows: 0, 100, 300, 600 and 900 mM. At 28, 42, 84, 98, 146 and 160 d after transplanting, plants were irrigated with one liter of the particular treatment for each application. Dry matter yield of napiergrass was not affected (p>0.05) by salinity treatments. Plants treated with 100 mM NaCl exhibited a higher soluble oxalate content compared to other treatments, but the differences were not statistically significant (p>0.05). Although salinity treatments had significant (p<0.05) effects on insoluble and total oxalate contents in plant tissue between the 100 and 900 mM NaCl treatments, the differences were too small to be considered biologically important. The present study indicates that where the soil is high in NaCl, napiergrass will tend to grow well and be low in oxalate.

Molecular Isolation and Characterization of the 2CysPrx Gene from Salicornia herbacea (퉁퉁마디로부터 2CysPrx 유전자 분리 및 특성 분석)

  • Kim, Suk-Kyu;Chung, Sang Ok;Na, Gil-Jong
    • Korean Journal of Environment and Ecology
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    • v.30 no.5
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    • pp.810-820
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    • 2016
  • This study is focused on the investigation of the genes which are induced by various stresses of the halophyte Salicornia herbacea. One of the factors influencing in the germination of Salicornia herbacea is salt stress. The highest germination rate was found in the condition without NaCl, and the upper limit of the NaCl concentration for the germination of Salicornia herbacea was 7%. The optimal temperature of $20^{\circ}C$showed a germination rate of 98%. Among genes induced by stress the 2CysPrx gene was cloned and analyzed for this study. The 2CysPrx gene has two cysteine conserved residues and is composed of 275 amino acids with molecular weight of 30.1kDa. The 2CysPrx gene appeared to be one copy in the genome and consists of 6 introns and 7 exons. Quantitative real-time PCR revealed that the highest transcription rate induced by NaCl and $H_2O_2$ appeared to be at the concentration of 3.5% NaCl and 40mM $H_2O_2$, respectively. The amount of transcript induced by high temperature($40^{\circ}C$) and $75{\mu}M$ of ABA was respectively highest. The gene at low temperature ($4^{\circ}C$) appeared not to be expressed. We are conducting to clone other peroxyredoxin genes induced by various environmental stresses.

Presoaking with $GA_3$ Improves Germination of Zoysiagrass (Zoysia japonica Steud.) Seed on Poor Germination Conditions (발아불량 환경조건에서의 $GA_3$ 침지처리에 의한 들잔디의 종자발아촉진)

  • 구자형;윤병한
    • Asian Journal of Turfgrass Science
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    • v.13 no.1
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    • pp.21-28
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    • 1999
  • The seeds of Zoysiagrass (Zoysia japonica Steud.) were soaked with GA3 50 ppm and primed with CaCl2 at -1.0 MPa for 4days at 23$^{\circ}C$ to identify presoaking and priming regimes that may improve germination in saline condition and with PEG 8000 at high temperature. Presoaked, primed, and untreated seeds were then germinated at 30 and $35^{\circ}C$. NaCl salinity stress consistently decreased the rate of germination of zoysiagrass seed. GA3 or CaCl2 alleviated the inhibitory effect of salinity on germination. However, total percent germination (G) and T50 of untreated control seeds significantly decreased and prolonged at $30^{\circ}C$ and $35^{\circ}C$ as NaCl salinity stress increased. Presoaked seeds with GA3 50 ppm for 4 days at $23^{\circ}C$ had significantly higher germination and lower T50 than untreated or primed with CaCl2 at -1.0 MPa for 4days at $23^{\circ}C$m and overcame the inhibitory effect of germination derived from PEG 8000. In addition, presoaked seed had higher, faster, and more uniform germination than untreated seeds after sowing in growing media in greenhouse.

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Molecular Cloning of Maltooligosyltrehalose Trehalohydrolase Gene from Nostoc flagelliforme and Trehalose-Related Response to Stresses

  • Wu, Shuangxiu;He, Liang;Shen, Rongrong;Zhang, Xiu;Wang, Quanxi
    • Journal of Microbiology and Biotechnology
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    • v.21 no.8
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    • pp.830-837
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    • 2011
  • A genomic DNA fragment encoding a putative maltooligosyltrehalose trehalohydrolase (NfMTH) for trehalose biosynthesis was cloned by the degenerate primer- PCR from cyanobacterium Nostoc flagelliforme. The ORF of NfMTH is 1,848 bp in length and encodes 615 amino acid residues, constituting a 70 kDa protein. The deduced amino acid sequence of NfMTH contains 4 regions highly conserved for MTHs. By expression of NfMTH in E. coli, the function of this protein was demonstrated, where the recombinant protein catalyzed the hydrolysis of maltooligosyl trehalose to trehalose. The expressions of MTH and maltooligosyltrehalose synthase in the filaments of N. flagelliforme were upregulated significantly under dehydration stress, NaCl stress, and high temperature-drought stress. The accumulations of both trehalose and sucrose in the filaments of N. flagelliforme were also improved significantly under the above stresses. Furthermore, trehalose accumulated in smaller quantities than sucrose did when under NaCl stress, but accumulated in higher quantities than sucrose did when under temperature-drought stress, indicating that both trehalose and sucrose were involved in N. flagelliforme adapted to stresses and different strategies conducted in response to various stress conditions.