• Title/Summary/Keyword: Na-P1

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Regulation of Intracellular pH by SHC1 in Saccharomyces cerevisiae (효모에서 SHC1 유전자의 이온 농도 조절에 의한 세포내 pH 항상성 유지)

  • 하승길;전준철;최의열
    • Korean Journal of Microbiology
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    • v.38 no.3
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    • pp.168-172
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    • 2002
  • Budding yeasts maintain an effective system to regulate intracellular pH in response to environmental pH fluctuation. In a previous study we reported that SHC1 plays a role in cell growth at alkaline condition, not at acid pH. We constructed a null mutant deleted an entire open reading frame for SHC1. To test whether the retardation in cell growth was caused by the absence of intracellular pH buffering capacity, we measured intracellular pH with a pH-sensitive fluorescent dye, C.SNARE. The intracellular pH of the mutant cell was much higher than that of wild-type cells, indicating that the mutant cells lack some types of buffering capacity. We also investigated the level of $Na^+ and K^+$ content with atomic mass spectroscopy after alkali shock. Wild-type cell showed a higher level of intracellular K^+$ content, whereas there was no difference in $Na^+$ level. The result suggested that K^+$ is more important in the regulation of intracellular pH in yeasts.

Occurrence of Syzygites Megalocarpus on Wild Mushrooms and Its Cultural Characteristics (야생(野生)버섯의 기생균(寄生菌)인 이분지털곰팡이의 출현(出現)과 배양(培養) 특성(特性))

  • Ka, Kang-Hyeon;Park, Hyun;Yoon, Kab-Hee;Bak, Won-Chull
    • Journal of Korean Society of Forest Science
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    • v.89 no.5
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    • pp.691-696
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    • 2000
  • Syzygites megalocarpus occurred on the fruit bodies of Tricholoma matsutake, Leccinum rugosiceps, Pulveroboletus ravenelii, Russula emetica, and Amanita pseudoporphyria in the field. We could select Malt Extract Agar, Mueller Hinton Medium and Potato Dextrose Agar as optimal media among eight media. Syzygites megalocarpus showed an optimal temperature around $23^{\circ}C$ with optimal pH 6.0. Growth of S. megalocarpus on PDA was inhibited by 36.5% at 5% NaCl compared with without NaCl and did not grow at more than 8% NaCl in 2 days after inoculation. However, it grew 1.1 cm in 10% NaCl in 5 days after inoculation. Growth of Tricholoma matsutake on PDA was inhibited by increasing contration of NaCl and did not nearly grow at 2.5% NaCl in 60 days after inoculation. Because S. megalocarpus grew at high concentration of NaCl, we concluded that NaCl should not use for controlling S. megalocarpus on the fruit body of T. matsutake.

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Changes in Protein Extractability, Thiobarbituric Acid and Volatile Basic Nitrogen of Spent Layer Meat Treated with Sodium Chloride and Phosphates (소금과 인산염 첨가에 따른 산란노계육의 단백질 추출성, Thiobarbituric Acid 및 Volatile Basic Nitrogen의 변화)

  • 박구부;이정일;신택순;진상근;문점동
    • Korean Journal of Poultry Science
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    • v.21 no.4
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    • pp.249-256
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    • 1994
  • This study was conducted to investigate the effects of the addition of varied levels of NaCI and phosphates to the breast meat of spent layers(2 \pm 0.2 kg), which were stabilized for over 24 h before slaughter, on the protein extractability, thiobarbituric acid(TBA) and volatile basic nitrogen (VBN). Within 1 h after slaughter, breast meat was removed and treated with NaCI(0, 1, 2, 3%) and phosphates(0.25% and 0.5%) using a hot-salted method. The breast meat samples were stored at 4\pm$1^{\circ}C$ for 3 d. The results obtained were summarized as follows. 1. Soluble protein contents of salt-treated groups were significantly higher than that of control (P<0.05) and showed a positive relationship with the levels of salt. At a constant level of NaCI, the soluble protein content was proportionately elevated by the levels of phosphates (P<0.05). It decreased significantly in both control and salt-treated groups during storage (P<0.05). 2. TBA values of salt-treated groups were significantly higher than that of control(P<0.05) and showed a positive relationship with the levels of salt. At a constant level of NaCI, TBA values in 0.5% phosphates treatment groups were significantly lower than that in 0.25%(P<0.05). It increased significantly in both control and salt-treated groups during storage(P<0.05). 3. VBN values of salt-treated groups were significantly lower than that of control(P<0.05) They increased significantly by the salt treatment for the first day of storage(P<0.05), but not from the second day of storage. VBN values in both control and salt-treated groups were significantly increased during storage(P<0.05). After the first day of storage and at the same level of NaCI, no significant difference in VBN value was observed between the two levels of phosphates.

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The Effect of Denentia prevention nursing program on Urinary 17-KS, 17-OHCS, and Blood Na, Cl, K, Ca, P, cholesterol of the Demented elderly women (치매간호중재 프로그램이 경중 치매노인의 Na, K, Ca, Cl, P, Cholesterol의 혈중 농도 및 17-KS, 17-OHCS의 뇨배설량에 미치는 영향)

  • Na, Hyun-Ju;Cho, Myung-Sook;Im, Wook-Bin
    • Journal of Korean Biological Nursing Science
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    • v.4 no.2
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    • pp.151-166
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    • 2002
  • This study was conducted for the evaluation of the effect of Dementia prevention nursing program on Urinary 17-KS, 17-OHCS, and Blood Na, Cl, K, Ca, P, cholesterol. The sample for the present study was composed of 16 demented elderly in D care center for the elderly in K city. The Dementia prevention nursing program consisted of concept memory training, music therapy, and art therapy. The program was performed twice a week and about ninety minutes was consumed for one session. The program had been administered for 8 weeks. We checked Urinary 17-KS, 17-OHCS, and Blood Na, Cl, K, Ca, P, cholesterol before and after the program. Statistical analysis was performed by using descriptive statistics and Wlicoxon signed rank test of SAS system for window 6.12. The result were follows : 1. Urinary 17-KS 17-KS score increased significantly after intervention(p=0.005). 2. Urinary 17-OHCS 17-OHCS score increased significantly after intervention(p=0.001). 3. Blood Na, Cl, K, Ca, P, cholesterol Na(p=0.0002), Cl(p=0.0001), K(p=0.0001), Ca(p=0.0028), decreased significantly after intervention. The results show that Dementia prevention nursing program increases Urinary 17-KS, 17-OHCS, and decreases Blood Na, Cl, K, Ca, P, cholesterol. In conclusion, the Dementia prevention nursing program can be used for the effective measure to decrease stress of the Demented elderly.

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Effects of Dietary Addition of Bentonite on Manure Gas Emission, Health, Production, and Meat Characteristics of Hanwoo (Bos taurus coreanae) Steers

  • Lee, Sang-Moo;Kim, Young-Il;Kwak, Wan-Sup
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.12
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    • pp.1594-1600
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    • 2010
  • A study was conducted to determine the dietary effects of a clay mineral (sodium bentonite, NaB) on manure gas emission, health, production, and meat characteristics of Hanwoo steers. Two diets fed to steers included a control diet (concentrate mix and rice straw) and a treatment diet (control diet+1.0% clay mineral/concentrate mix). Dietary NaB addition considerably reduced concentrations of gases ($H_2S$, $SO_2$ and $NH_3$) in the manure of Hanwoo steers. Growing steers fed NaB had similar blood profiles with the exception of lower (p<0.05) concentrations of blood alkaline phosphatase and lactate dehydrogenase. Dietary NaB addition tended to increase (p = 0.10) live weight by 30 kg at slaughtering and did not affect carcass yield and quality traits. Dietary NaB addition increased concentrations of P (p<0.01), Mg (p<0.01), Na (p<0.01), Zn (p<0.005), K (p = 0.08), Fe (p = 0.08) and Cu (p = 0.07) in the longissimus muscle compared to the control but did not affect (p>0.05) fatty acid composition. The study demonstrated that the dietary addition of a clay mineral could be effective in improving mineral bioavailability to Hanwoo steers, which could be one of the reasons for their improved performance.

Effects of Co-P Catalysts on Hydrogen Generation Properties from Alkaline $NaBH_4$ Solution (알칼리 $NaBH_4$ 용액의 수소발생특성에 미치는 Co-P 촉매의 영향)

  • Cho, Keun-Woo;Kwon, Hyuk-Sang
    • Journal of Hydrogen and New Energy
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    • v.16 no.4
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    • pp.379-385
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    • 2005
  • Effects of Co and Co-P catalysts on the hydrolysis of alkaline $NaBH_4$ solution were investigated. Co and Co-P catalysts were prepared on Cu substrate by electroplating. Hydrogen generation rate of Co-P catalyst was much faster than that of Co catalyst, demonstrating that Co-P had higher intrinsic catalytic activity for the hydrolysis of $NaBH_4$ than Co. Hydrogen generation properties of Co-P catalysts largely depended on cathodic current density and electroplating time because they influenced on the P concentration of the Co-P catalysts. Maximum hydrogen generation rate of Co-P catalyst was 1066 ml/min.g-catalyst in 1 wt.% NaOH + 10 wt.% $NaBH_4$ solution at $20^{\circ}C$, which was obtained at cathodic current density of $0.01\;A/cm^2$ for 130 s.

Determination of the Effect of Feeding Different Sodium Chloride Regimes on Growth Performance, Viscera Organ Weights and Meat Quality Parameters of Broilers from Hatch to 35 Days of Age

  • Vinothsankar, Geerththana;Macelline, Shemil Priyan;Wickramasuriya, Samiru Sudharaka;Nawarathna, Shan Randima;Ang, Li;Tharangani, Himali;Jayasena, Dinesh Darshaka;Heo, Jung Min
    • Korean Journal of Poultry Science
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    • v.47 no.2
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    • pp.75-82
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    • 2020
  • This study intended to investigate the growth performance and meat quality traits in broilers fed different regimens of NaCl over a period of 35 days. Seven hundred and fifty 1-day-old chicks (Cobb 500) were randomly assigned to five NaCl feeding regimens to obtain three replicates for each regimen. Broilers in CON group were provided with concentrations of 0.35% NaCl for the duration of the experimental period. The other groups were provided with two concentrations of NaCl (0.45% and 0.55%) under two durations, namely: 1) 1~28 d: 0.45% or 0.55% NaCl for 28 days then CON; and 2) 1~35 d: 0.45% or 0.55% NaCl for 35 days. Broilers fed 0.55% NaCl for 1~35 d showed improved (P<0.05) growth performance compared with broilers fed the CON regimen. Moreover, higher water holding capacity of breast meat was shown by the broilers fed 0.55% NaCl for 1~28 d when compared with that in broilers fed the CON diet (P<0.05). Broilers fed 0.45% NaCl showed an increase (P<0.05) in the lightness of the meat over CON diet and the 0.55% NaCl diet. In addition, broilers offered CON had the highest redness value and highest crude ash content in the breast meat (P<0.05). Feeding 0.45% NaCl for 1~35 d and 0.55% NaCl for 1~28 d resulted in a higher (P<0.05) protein content in breast meat. In conclusion, broilers fed diets with 0.55% NaCl for 1~35 d showed improved growth performance as well as water holding capacity and protein content of the breast meat.

Synthetic Study of Zeolites from Some Glassy Rocks (II) : Dissolution Behavior of Perlite and Zeolite Synthesis in Alkaline Aqueous Solution (유리질 암석으로부터 제올라이트 합성에 관한 연구(Ⅱ) : 알칼리 용액에서 진주암의 용해 거동과 제올라이트의 합성)

  • Noh, Jin-Hwan
    • Journal of the Mineralogical Society of Korea
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    • v.5 no.2
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    • pp.61-71
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    • 1992
  • Through the low-temperature(60-150${\circ}C$) hydrothermal treatment of perlite with the alkaline solution at various NaOH concentrations, the mode of volcanic glass alteration and resultant zeolite formation were investigated in a closed system. At a temperature of 80${\circ}C$ and alkalinities of pH range 8 to 12, corresponding to the natural environments of diagenetic zeolite formation, only weak dissolution of perlitic glass occurs without zeolite formation despite the residence time of 100 days. Activities of Si and Al increase progressively, as a consequence of increasing pH, whereas activity ratios of Si/Al decrease. Zeolites were synthesized from perlite in the alkaline solution at above 0.1M NaOH concentrations. Below the temperature of 100${\circ}C$ Na-P was mainly formed, whereas analcime was the dominant zeolite at the temperature range of 100-150${\circ}C$. During Na-P synthesis chabazite and Na-X were also formed as by-products in case of lower proportion of solution/sample(<10ml/g) and higher NaOH concentraion (>3M), respectively. The alteration modes of perlite in the zeolite synthesis reflect that the formation of synthetic zeolites occurs as an incongruent dissolution likely with the diagenetic formation of natural zeolites from volcanic glass. Considering much difference in reaction kinetics between natural and synthetic systems, however, the evaluated synthetic conditions in these experiments were not directly applicable to the natural diagenetic system.

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Reduction of N-Arylpyridinium Compounds by Sodium Borohydride and Dithionite: Regioselectivity and Isomerization of Reduction Products

  • Koh, Park, Kwang-Hee;Han, Du-Hee;Shin, Dae-Hyun
    • Bulletin of the Korean Chemical Society
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    • v.7 no.3
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    • pp.201-204
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    • 1986
  • Reduction of N-arylpyridinium compounds by $NaBH_4$ gave mixtures of the corresponding 1,2-dihydropyridine(major) and 1,4-dihydropyridine(minor), whereas similar reduction by $Na_2S_2O_4$ produced 1,4-dihydropyridines regioselectively. The proportion of 1,4-isomer in the product by $NaBH_4$ reduction appeared to increase with the electron-donating ability of N-aryl groups. When the N-aryl group is p-methylphenyl, p-ethylphenyl or p-methoxyphenyl, the 1,2-dihydropyridines in ethanol-water (4:1) solutions isomerized to the corresponding 1,4-dihydropyridines. N-(p-methylphenyl)-1,2-dihydropyridine and N-(p-ethylphenyl)-1,2-dihydropyridine in solid state also isomerized to the corresponding 1,4-dihydropyridines. The different behaviors of reduction among N-arylpyridiniums and isomerization of the reduction products depending on the substituent in N-aryl group were explained in terms of difference in the electronic effects of the substituents.

A Study on the Treatment of Soil Flushing Effluent Using Electrofloatation : Effects of Electrolyte and pH (전기부상을 이용한 토양세정 유출수 처리에 관한 연구 : 전해질 및 pH의 영향)

  • 소정현;최상일;조장환
    • Journal of Soil and Groundwater Environment
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    • v.8 no.3
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    • pp.56-60
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    • 2003
  • The optimal operation conditions of electrofloatation for oil-water separation of soil flushing effluent including electrolyte and pH were investigated. The reactor (200 ${\times}$ 10 ${\times}$ 15 cm) for the experiment was constructed by using acrylic plate. Diesel concentration was 1,000 mg/L in the 1 % mixed surfactant solution ($POE_5$: $POE_{14}$ 1: 1). Titanium coated electrode was used as cathode and stainless steel electrode as anode. Reaction time was 62 minutes (reaction time: 60 min., flotation time: 2 min.) and voltage was 6 V. The separation efficiency of electrofloatation was improved to 40% by electrolyte addition. Furthermore, NaCl (1N) added as electrolyte was showed enhanced efficiency compared to NaOH (1N). While, the effect of both NaCl and NaOH was sequentially increased in the range of 0.2∼1.0% (0.02∼0.1 M). The equilibrium time was found as 20 min. in the range of 0.4∼1.0% (0.04∼0.1M) for both of them.