• Maxillofacial Plastic and Reconstructive Surgery
• /
• 제11권1호
• /
• pp.171-186
• /
• 1989

This study was undertaken to investigate the effects of indomethacin on 4-nitroquinoline 1-oxide (4NQO) induced palatal carcinoma of albino rats. Sixty albino rats about 100 gms of body weight, 6 weeks old-were used classifying as 1) six albino rats of normal group received no treatment, 2) six albino rats of control group treated with propane 1, 2-diol 3 times a week, 3) twenty four albino rats of experimental group I treated with 0.5% 4NQO in propane 1, 2-diol 3 times a week, 4) twenty four albino rats of experimental group II treated with 0.5% of 4NQO in propane 1, 2-diol 3 times a week and administrated 20${\mu}g/ml$ indomethacin in drinking water ad lib. The animals of normal and control groups were sacrificed 7th, 11th, 15th, 19th, 23rd and 27th week, while those of experimental group I and II were sacrificed 7th, 9th, 11th, 13th, 15th, 17th, 19th, 21st, 23rd, 25th, 27th and 29th week after the experiment. The palatal mucosa was excised and examined grossly, light-microscopically and electron-microscopically. Following results were obtained. 1. In control group, there was no specific difference from normal tissue histopathologically. 2. In group I, hyperkeratosis mild acantosis and dyskeratosis in in 7th week, dysplasia in 11th week and severe acantosis in 19th week were observed, but squamous cell carcinoma not observed until 29th week on light-microscope. 3. In group II, hyperkeratosis, mild acantosis in 9th week, dyskeratosis and dysplasia in 21st week, severe acantosis in 27th week and squamous cell carcinoma in 29th week were observed on light-microscope. 4. In group I, widening of intercellular space in 7th week, increasing of desmosome, giant desmosome and tonofilament in cytoplasm in 9th week, severe widening of intercellular space, increasing of mitochondria and vascular degeneration in 11th week, irregular pattern of cell feature and nucleus and prominent nucleoli in 19th week, and continuity of basal lamina in 29th week were observed on electron-microscope. 5. In group II, mild widening of intercellular space in 9th week, increasing of mitochondria, vascular degeneration and tonofilament in cytoplasm in 13th week, increasing of desmosome and giant desmosome in 15th week, irregular pattern of cell surface and nucleus and prominent nucleoli, and in 21st week continuity of basal lamina were observed on electron-microscope which phenomenon occurred little later than group I. After 21st week, however, severe widening of intercellular space, vascular degeneration and continuity of basal lamina were observed as in group I.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• 제11권1호
• /
• pp.187-202
• /
• 1989

This study was undertaken to investigate the effect of indomethacin on prostaglandins in 4-Nitroquinoline-N-Oxide (4-NQO) induced palatal carcinoma of albino rats. 128 Sprague-Dawley strain albino rats-about 100g in body weight-were used in this study, divided into as belows; 1. Normal group (16-albino rats) with no treatment, 2. Control group (16-albino rats) treated with prophylene application onto palatal mucosa 3 times a week. 3. Experimental group I (48-albino rats) treated with 0.5% 4-NQO in prophylene application onto palatal mucosa 3 times a week. 4. Experimental group II (48-albino rats) treated with 0.5% 4-NQO in prophylene application with administered $20{\mu}g/ml$ of indomethacin in drinking water ad. lib. Four animals were sacrificed 7th, 13th, 19th, and 25th week respectively in normal and control group, and 7th, 9th, 11th, 13th, 15th, 17th, 19th, 21st, 23rd, 25th, 27th and 29th week respectively in experimental group I and II at each time. The palatal and lingual tissues were excised and kept frozen at $-70^{\circ}C$. Densitometer scan and Beta-counting counter were used for the thin layer chromatography of the arachidonic acid metabolites. The obtained results were as belows; 1. In normal and control group, there was little change of the arachidonic acid metabolites during experiment period, and the tissue homogenates included prostaglandin $D_2$, 6-keto-prostaglandin $F_{1{\alpha}}$, prostaglandin $E_2$, thromboxane $B_2$, prostaglandin $F_{2{\alpha}}$ in that order of relative abundances. 2. In experimental group I, prostaglandin $D_2$, and prostaglandin $E_2$ were increased, while 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$ were decreased in relative abundances of arachidonic acid metabolites. And there was little change in prostaglandin $F_{1{\alpha}}$ 3. In experimental group II, prostaglandin $D_2$, and prostaglandin $E_2$ were increased, while 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$ were decreased in relative abundances of arachidonic acid metabolites. And there was little change in prostaglandin $F_{2{\alpha}}$ also. 4. In the range of increase in prostaglandin $D_2$, and prostaglandin $E_2$, and that of decrease in 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$, in relative abundances, there was wider in experimental group I than in group II. 5. In the range of increase in prostaglandin $D_2$, and prostaglandin $E_2$, and that of decrease in 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$, in relative abundances, there was wider in palatal mucosa than in lingual mucosa in experimental group I and II.

• Radiation Oncology Journal
• /
• 제31권2호
• /
• pp.57-65
• /
• 2013

Beta-lapachone (${\beta}$-Lap; 3,4-dihydro-2, 2-dimethyl-2H-naphthol[1, 2-b]pyran-5,6-dione) is a novel anti-cancer drug under phase I/II clinical trials. ${\beta}$-Lap has been demonstrated to cause apoptotic and necrotic death in a variety of human cancer cells in vitro and in vivo. The mechanisms underlying the ${\beta}$-Lap toxicity against cancer cells has been controversial. The most recent view is that ${\beta}$-Lap, which is a quinone compound, undergoes two-electron reduction to hydroquinone form utilizing NAD(P)H or NADH as electron source. This two-electron reduction of ${\beta}$-Lap is mediated by NAD(P)H:quinone oxidoreductase (NQO1), which is known to mediate the reduction of many quinone compounds. The hydroquinone forms of ${\beta}$-Lap then spontaneously oxidizes back to the original oxidized ${\beta}$-Lap, creating futile cycling between the oxidized and reduced forms of ${\beta}$-Lap. It is proposed that the futile recycling between oxidized and reduced forms of ${\beta}$-Lap leads to two distinct cell death pathways. First one is that the two-electron reduced ${\beta}$-Lap is converted first to one-electron reduced ${\beta}$-Lap, i.e., semiquinone ${\beta}$-Lap $(SQ)^{{\cdot}-}$ causing production of reactive oxygen species (ROS), which then causes apoptotic cell death. The second mechanism is that severe depletion of NAD(P)H and NADH as a result of futile cycling between the quinone and hydroquinone forms of ${\beta}$-Lap causes severe disturbance in cellular metabolism leading to apoptosis and necrosis. The relative importance of the aforementioned two mechanisms, i.e., generation of ROS or depletion of NAD(P)H/NADH, may vary depending on cell type and environment. Importantly, the NQO1 level in cancer cells has been found to be higher than that in normal cells indicating that ${\beta}$-Lap may be preferentially toxic to cancer cells relative to non-cancer cells. The cellular level of NQO1 has been found to be significantly increased by divergent physical and chemical stresses including ionizing radiation. Recent reports clearly demonstrated that ${\beta}$-Lap and ionizing radiation kill cancer cells in a synergistic manner. Indications are that irradiation of cancer cells causes long-lasting elevation of NQO1, thereby sensitizing the cells to ${\beta}$-Lap. In addition, ${\beta}$-Lap has been shown to inhibit the repair of sublethal radiation damage. Treating experimental tumors growing in the legs of mice with irradiation and intraperitoneal injection of ${\beta}$-Lap suppressed the growth of the tumors in a manner more than additive. Collectively, ${\beta}$-Lap is a potentially useful anti-cancer drug, particularly in combination with radiotherapy.

• 한국식품저장유통학회지
• /
• 제15권2호
• /
• pp.274-279
• /
• 2008

해양심층수 간장 및 일반간장을 제조하여 항돌연변이원성과 세포독성을 측정하였으며 sarcoma-180 cell을 이용하여 in vivo에서 항암효과를 살펴보았다. S. typhimurium TA98과TA100 균주를 이용한 실험에서 모든 시료에서 돌연변이원성이 없었으며, 항돌연변이원성 실험에서는 직접변이원인 MNNG($0.4{\mu}g$/plate)의 경우 TA100 균주에서 해양심층수간장의 시료농도 $200{\mu}g$/plate에서 90.9%의 높은 억제효과를 나타내었으며 4NQO($0.15{\mu}g$/plate)에 대해서는 같은 시료농도에서 62.0%의 억제효과를 나타내었다. 그리고 4NQO의 경우 TA98 균주에 대해서 해양심층수 간장은 61.7%의 억제효과를 나타내었으며 모든 시료는 농도 의존적으로 억제하는 것으로 나타났다. 세포독성 효과를 알아보기 위하여 HeLa, Hep3B, AGS, A549와 MCF-7을 사용하였다. 각 시료 추출물의 암세포 성장효과를 조사한 결과, 해양심층수 간장이 1 mg/mL의 농도에서 각각 69.4%, 70.5%, 55.6%, 82.1% 및 73.2%의 억제율을 나타내었다. 그리고 해양심층수 간장은 고형암 성장 억제 실험에서 대조군에 비해서 40.9%의 고형암 성장 억제효과를 나타내었다.

• 한국식품과학회지
• /
• 제32권6호
• /
• pp.1371-1378
• /
• 2000

S. typhimurium TA98과 TA100을 이용한 Ames test에서는 아가리쿠스버섯 메탄올 추출물 모두 시료자체의 돌연변이원성은 없는 것으로 나타났다. 아가리쿠스버섯 메탄올 추출물$(200\;{\mu}g/plate)$ 중 TA98 균주를 이용한 항돌연변이 효과를 확인한 결과 직접변이원인 4NQO에 대해 92.4% 그리고 간접변이원인 Trp-P-1과 $B({\alpha})P$에 대해 각각 81.9%와 83.4%의 높은 억제효과를 나타내었다. 또한 TA100 균주에서 4NQO는 94.7%의 가장 높은 억제효과를 나타내었다. MNNG, Trp-P-1 및 $B({\alpha})P$은 각각 87.3%, 89.9% 그리고 92.3%의 억제율을 보였다. 각각의 변이원 물질에 대한 아가리쿠스버섯 분획물 $(200\;{\mu}g/plate)$의 항돌연변이 효과에서는 MNNG와 $B({\alpha})P$에 대해서는 분획물 모두가 80% 이상의 높은 억제율을 보였고, 4NQO는 TA98, TA100 두 균주 모두 에틸 아세테이트 분획물에서 95% 이상의 가장 높은 억제율을 나타내었다. 그리고 Trp-P-1에서는 TA98 균주에서 물층을 제외한 분획물이 높은 억제율을 나타내었고, TA100 균주에 대해서도 분획물 모두가 80% 이상의 높은 억제율을 나타내었다. 각종 암세포에 대한 아가리쿠스버섯 메탄올 추출물의 저해효과는 시료 1 mg/mL 투여시 MCF7 82.9%, A549 86.5%, HT1080 65.5%, Hep3B 84.3%, HeLa 91.9%, KAROIII 88.7% 그리고 K562세포에서는 82.0%의 억제효과를 나타내었다. 인간 정상 간세포 WRL68에 대한 시료 농도에 따른 세포독성효과는 1 mg/mL의 시료를 첨가시 50% 이하의 생육억제율을 나타냄으로써 정상세포에 대해서는 낮은 독성효과를 나타낸다는 사실을 알 수 있었다. 아가리쿠스버섯 분획물들에 대한 억제효과에서는 유방암 세포인 MCF7이 시료를 1 mg/mL 첨가하였을때 물층과 부탄올층을 제외한 분획물에서 90% 이상의 높은 억제효과를 보였다. 또한 에틸 아세테이트 분획물이 다른 분획물에 비해 각각의 암세포에 대해서도 1 mg/mL 첨가시 80% 이상의 높은 억제효과를 보였다. 그러나 물 분획물은 암세포에 대해 상대적으로 낮은 억제효과를 나타내었다.

• 대한간호학회지
• /
• 제53권2호
• /
• pp.236-248
• /
• 2023

Purpose: Nursing informatics competency is used to manage and improve the delivery of safe, high-quality, and efficient healthcare services in accordance with best practices and professional and regulatory standards. This study examined the relationship between nursing informatics competency (NIC), nursing care left undone, and nurse reported quality of care (NQoC) and nursing productivity. A path model for their effects on nursing productivity among clinical nurses was also established. Methods: Data were collected using structured questionnaires answered by 192 nurses working in a tertiary hospital located in J city, Korea, and analyzed using SPSS/WIN 23.0 and AMOS 21.0 program. Results: The fit indices of the alternative path model satisfied recommended levels χ² = .11 (p = .741), normed χ² (χ² /df) = .11, SRMR = .01, RMSEA = .00, GFI = 1.00, NFI = 1.00, AIC = 18.11. Among the variables, NIC (β = .44, p < .001), NQoC (β = .35, p < .001) had a direct effect on nursing productivity. Due to the mediating effect of NQoC on the relationship between NIC and nursing productivity, the effect size was .14 (95% CI .08~.24). Meanwhile, nursing care left undone through NQoC in the relationship between NIC and nursing productivity, has a significant mediation effect (estimate .01, 95% CI .00~.03). The explanatory power of variables was 44.0%. Conclusion: Education and training for enhancing NIC should be provided to improve nursing productivity, quality of care and to reduce missed nursing care. Furthermore, monitoring the quality of nursing care and using it as a productivity index is essential.

• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
• /
• pp.176-176
• /
• 2002

In order to investigate whether the induction of micronucleus and aneuploidy in human lymphocytes by Hydroquinone (HQ) is associated with genetic polymorphisms of CYP1A1, GSTM1, GSTT1, NQO1 gene, the cytokinesis-block micronucleus (CBMN) assay in combination with fluorescence in situ hybridization (FISH) technique using specific centromeric probes for chromosome 7 and 8 and PCR-RFLP based genotyping for 30 healthy people were performed.(omitted)

• 대한한방내과학회지
• /
• 제25권1호
• /
• pp.106-118
• /
• 2004

Antigenotoxicity test (SOS chromotest), antimutagenecity test (Ames test), and antioxidant test (NBT method and xanthine-xanthine oxidase method) were carried out using water-soluble and methanolic extracts from Puerariae Flos. Against the mutagens MNNG and NQO, antigenotoxic activity of methanolic extracts were much more effective than that of water-soluble ones. When the methanolic extract was added to the certain concentration $(100{\mu}{\ell}/tube)$, antigenotoxic acivity against the mutagen MNNG was enhanced. Contrary to the water-soluble extract, the methanolic extract showed high antigenotoxicity against the mutagen NQO with increment of the extract. Against the mutagen MNNG with Ames test, antimutagenic activity of the methanolic extract at $300{\mu}{\ell}/tube$ was 96% as an inhibition ratio of revertant forming CFU/plate. The antioxidant activity of water-soluble extract was comparatively higher than that of the methanolic one.

• 대한임상약리학회:학술대회논문집
• /
• 대한임상약리학회 2001년도 제10차 추계학술대회
• /
• pp.51-52
• /
• 2001

• 대한통합의학회지
• /
• 제7권4호
• /
• pp.61-69
• /
• 2019

Purpose : Human gingival fibroblast cell is one of the the main cell types in periodontal tissue, which they can show anti-inflammatory activity through the production of numerous lines of inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and interleukins. Porphyromonas gingivalis, one of the oral pathogens, has reported to play a critical role in the development of periodontal diseases. This study aimed to investigate anti-inflammatory and antioxidative activities of Gracilaria textorii ethanol extract (GTEE) in P. gingivalis derived lipopolysaccharide (LPS-PG) stimulated human gingival fibroblast (HGF)-1 cell line. Methods : In order to analyze anti-inflammatory and antioxidative activities of GTEE in HGF-1 cell line, NOS enzyme activity, expression levels of iNOS, COX-2, NAD(P)H quinone dehydrogenase (NQO)1 and their transcription factors were estimated by Griess reaction and western hybridization. Results : LPS-PG induced overexpression of iNOS and COX-2, which was significantly attenuated by GTEE treatment in a dose-dependent manner without any cytotoxicity. In addition, intracellular NOS activity was in accordance with the result of iNOS expression. Due to important role in the regulation of inflammatory responses, phosphorylated status of p65 and c-jun, each subunit of nuclear factor (NF)-κB and activator protein (AP)-1, was also dose-dependently ameliorated by GTEE treatment. One of phase II enzymes, NQO1, and its transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2), were analyzed since elevated phase II enzyme expression inhibited inflammatory response, which was significantly elevated by GTEE treatment in HGF-1 cell line. Conclusion : In conclusion, GTEE mitigated LPS-PG-stimulated inflammatory responses by attenuating NF-κB and AP-1 activation as well as accelerating NQO1 and Nrf2 expression in HGF-1 cell line. These results indicate that GTEE might be utilized a promising strategy for potential anti-inflammatory agent in periodontal diseases.