• Mycobiology
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• v.46 no.4
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• pp.429-439
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• 2018

To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4'-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation in A. nidulans, as a new reporter gene. The functional organization of serially deleted promoter regions of the A. nidulans trpC gene and the Cryphonectria parasitica crp gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase npgA gene. Several promoter regions of the trpC and crp genes were fused to the npgA gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in A. nidulans to evaluate color recovery due to the transcriptional activity of the sequence elements. Serial deletion of the trpC and crp promoter regions in this PPTase reporter assay system reaffirmed results in previous reports by using the fungal transformation step without a laborious verification process. This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies.

• Polymer(Korea)
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• v.24 no.5
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• pp.589-598
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• 2000

In this study, various unsaturated polyester (UPE) resins were prepared from the condensation polymerization of mixtures of saturated (isophthalic acid : IPA) and unsaturated (maleic anhydride : MA) dibasic acids with propylene glycol (PG), neopentyl glycol (NPG). The critical surface tension (Υ$_{c}$) for the surface characteristics of a solid were estimated by Zisman plot, and the structure-property relationship was investigated by measuring the rheology of resins. The values of Υ$_{c}$ for glass of solid were 30.5 mNㆍm$^{-1}$ for UPE resin liquids. As the content of NPG in a PG/NPG glycol mixture increased, both the contact angle and the surface tension of the UPE resin liquids were found to decrease. The dynamic viscoelasticities of UPE resins with different glycol molar ratios were also measured. Shear rate dependence of viscosity and angular frequency dependence of storage, and loss modulus tended to decrease with increasing NPG content.

• Food Science of Animal Resources
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• v.33 no.1
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• pp.24-30
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• 2013

This study was conducted to compare the physicochemical and sensory properties of yogurt added with nanopowdered ginseng (NPG) and powdered ginseng (PG) of different concentrations (0.1, 0.3, 0.5, and 0.7%) (w/v) during the storage at $7^{\circ}C$ for 20 d. The pH and viscosity values of yogurt added NPG or PG decreased during the storage. The pH values of the yogurt samples were ranged from 4.0 to 4.6 as a reflective of the fresh state. Viscosity values of yogurt with NPG at lower concentrations 0.1 and 0.3% (w/v) showed higher values during increased storage time. DPPH radical scavenging activity was significantly higher in the NPG-added yogurt than in the PG during the storage period (p<0.05). The lactic acid bacteria (LAB) counts ranged from $3.0{\times}10^9$ to $1.3{\times}10^9$ and $2.2{\times}10^9$ to $1.1{\times}10^9CFU/mL$ in 0.3% NPG and PG-added yogurts, respectively. Increased storage period showed decrease in LAB counts irrespective of the type of ginseng powder and storage period. In sensory test, 0.1 and 0.3% NPG-added yogurt showed similar results to control in yellowness, viscosity, and bitterness. Based on the data obtained from the present study, it was concluded that the concentrations 0.1 and 0.3% (w/v) of NPG could be used to produce NPG-added yogurt without significant adverse effects on physicochemical and sensory properties, and enhance functional value of yogurt.

• Korean Journal of Materials Research
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• v.2 no.2
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• pp.101-109
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• 1992

Polyethylene terephthalate(PET) is a well known commercial polymer widely available as fibre and as a major polymer with diverse applications. In this experiment, for the modification of PET, the pentanediol isomers, 1,5-pentanediol(1,5-PD) and neopentyl glycol(NPG), were introduced as third monomers. Crystallization rate decreased by the added NPG of PET modified by NPG units. In the case of PET modified by 1,5-PD, crystallization rate had been slightly increased at all temperatures until the 1,5-PD was added about 10 mol%. The decreased crystallization rate is thought to be due to branched from methyl group of neopentyl, which greatly retards the crystallization by growing onto the crystal surface of the diffused polymer chain.

• The Korean Journal of Food And Nutrition
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• v.6 no.2
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• pp.109-114
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• 1993

This study was divised to observe an Inhibitory effect toward a lipolytic action of toxohormone-L from large root and small root Nepal pseudo ginseng (NPG ; Nepal products) components by water extract and ethanol precipitate in vitro. Toxohormone-L is known to be a lipolytic factor that was partially purified from the ascites fluid of Sarcoma 180-bearing mice and of patients with hepatoma. The inhibitory effect that inhibited the lipolytic action of toxohormone-L by ethanol Precipitate component of large root NPG (mean 55.5%) was higher (mean 1.37 times) than that of water extract component in final reaction concentration of 500 and 1, 000ug/ml, on the other side inhibitory effect of water extract component in small root NPG (mean 55.5%) was higer (mean 1.14 times) than that of ethanol precipitate component. In a way inhibitory effect of precipitate component In large root NPG(47.6%), when final reaction concentration of sample were 1, 000ug/ml, was about 40% lower than that of Korean red ginseng.

• Mass Spectrometry Letters
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• v.4 no.1
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• pp.21-23
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• 2013

The effects of trifluoroacetic acid (TFA) were evaluated on the generation of multiply charged ions of cytochrome c in a 2-nitrophloroglucinol (2-NPG) matrix in high-vacuum, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The presence of 1% TFA in the 2-NPG matrix solution was more effective in generating multiply charged protein ions than matrix solutions containing 0.1% or 0% TFA. Regarding the matrix itself, with 1% TFA, 2-NPG was significantly more effective in generating multiply charged ions than 2,5-dihydroxybenzoic acid (2,5-DHB). The maximum charge state of cytochrome c was +8 when using a 2-NPG matrix containing 1% TFA.

• The Korean Journal of Food And Nutrition
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• v.4 no.1
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• pp.75-80
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• 1991

This study was divised to observe an inhibitory toward a lipolytic action of toxohormone-L from large root and small root Nepal pseudo ginseng(NPG ; Nepal products) components by water extract and ethanol precipitate in vitro. Toxohormone-L Is known to be a lipolytic factor that was partially purified from the ascites fluid of sarcoma 180-hearing mice and of patients with hepatoma. The inhibitory effect that inhibited the lipolytic action of toxohormone-L by ethanol precipitate component of large root NPG(mean 46.8%) was higher (mean 1.8 times) than that of water extract component in final reaction concentration ,5001g1m1, on the other side inhibitory effect of water extract component in small root NPG(mean 43.9%) was higher(mean 1. 2 times) than that of ethano1 precipitate component, respectively. In a way inhibitory effect of ethanol precipitate component in large root NPG(47.6%), when final reaction concentration of sample were 1,000 U g/ml, was about 4095 lower than that of Korean red ginseng, respectively.

• Journal of Acupuncture Research
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• v.28 no.6
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• pp.149-157
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• 2011

Objectives : The purpose of this study is to investigate the effect of mixed pharmacopuncture of $Jungsongouhyul$ and $Cervi$ $Pantotrichum$ $Cornu$ on low back pain patients. Methods : 83 patients with Low back pain who were treated in Dept. of Acupuncture and Moxibusion, Dongshin University Hospital in Suncheon from Sep. 1st, 2010 to August 31th, 2011, were divided into two groups. One group (52 patients) was treated with conservative treatments (acupuncture, herb med, cupping therapy and physical therapy) and mixed pharmacopuncture of $Jungsongouhyul$ and $Cervi$ $Pantotrichum$ $Cornu$ theraphy (PG) and the other group (31 patients) was treated with conservative treatments alone(NPG). To estimate the effects of treatments, visual analog scale(VAS) and Oswestry disability index(ODI) were checked. Results : 1. In VAS, both of PG and NPG shows statistically significant improvement rate after 3rd treatment and 5th treatment. 2. In ODI, both of PG and NPG shows statistically significant improvement rate after 3rd treatment and 5th treatment. 3. In comparing VAS of PG and NPG, there was more significant improvement in PG than NPG after 3rd treatment and 5th treatment. 4. In comparing ODI of PG and NPG, there was more significant improvement in PG than NPG after 3rd treatment but no significant improvement between PG and NPG after 5th treatment. Conclusions : According to the study, it is thought that mixed pharmacopuncture of $Jungsongouhyul$ and $Cervi$ $Pantotrichum$ $Cornu$ theraphy is effective to low back pain patients, and further study is needed for the confirmation of the effect of mixed pharmacopuncture of $Jungsongouhyul$ and $Cervi$ $Pantotrichum$ $Cornu$.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.41-50
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• 2007

Data from a comparative slaughter experiment with two hundreds of Korean native (Hanwoo) beef steers were utilized to determine net energy and protein requirements for growth (NEg and NPg). Eight randomly selected steers were used in the initial slaughter group of 6 month of age and the remaining steers allocated to treatments within two groups. The restricted groups were fed 1.2 to 1.5% concentrate per kg body weight (BW) in the growing period (6～12 month of age), 1.7 to 1.8% concentrate per kg BW in the early fattening period (13～18 month of age), and concentrate feed ad libitum in the late fattening period (19～30 month of age). Ad libitum groups were fed concentrate ad libitum through the whole period. Rice straw was offered ad libitum for 24 month of two feeding systems. The steers were slaughtered when animals reached every 2 month from 8 to 30 month of age. For all body composition determinations, whole empty body components were weighed, taken each proportional subsample was ground for chemical analysis. Equations developed with the pooled data to predicted NEg and NPg were similar to the equations of Japanese Feeding Standard for Beef Cattle (2000). First equations were developed to predicted NEg; NEg = 0.05332×LBW0.75×DG for restricted treatment and NEg = 0.04912×LBW0.75×DG for ad libitum treatment. Second equations were developed to predicted NPg; NPg = DG × (224.7－0.251×LBW) for restricted treatment and NPg=DG×(210.1－0.214×LBW) for ad libitum treatment.

• Korean Journal of Microbiology
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• v.34 no.3
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• pp.120-125
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• 1998

A plasmid pNPG contains a genomic DNA complementing npgA1 which is located on the left arm of linkage group I. It transformed Aspergillus nidulans at a high frequency. No abortive transformants were observed and the $Trp^+$ transformants were all $Npg^+$. The 10.4 kb Psti fragment of the genomic DNA was subcloned into pILJ16, which increased the transformation efficiency by more than 200-folds. The transformants were mitotically unstable and yielded $Arg^-$ conidia at the frequency of more than 80%. An additional gene cloned into the plasmid containing the fragment was always lost with $argB^+$ marker. These characteristics strongly indicate the possibility that the plasmids autonomously replicate. The full activity of enhanced transformation was retained on the 4.9 kb EcoRI-HaeIII fragment. The DNA segment was similar to AMA1 rather than ANS1 in function and designated AMA2.