• Proceedings of the KSME Conference
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• 2007.05a
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• pp.706-711
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• 2007

Numerical studies were performed to examine the effects of notch location of impact specimens on the failure behavior of HAZ (heat affected zone) when Charpy V-notch impact test were made at a low temperature ($1^{\circ}C$). Carbon steel plate (SA-516 Gr. 70) with thickness of 25mm for pressure vessel was welded by SMAW (shielded metal-arc welding) and specimens were fabricated from the welded plate. Charpy tests were then performed with specimens having different notch positions of specimens varying from the fusion line through HAZ to base metal. A series of finite element analysis which simulates the Charpy test and crack propagation initiating at the tip of V-notch was carried out as well. The finite element analysis takes into account the irregular fusion line and non-homogenous material properties due to the notch location of the specimen in HAZ. Results reveals that the energies absorbed during impact test depend significantly on the notch location and direction of specimen. Finite element analysis also demonstrates that the notch location of specimens, to a great extent, influences the reliability and consistency of the test.

• Archives of Plastic Surgery
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• v.37 no.1
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• pp.7-11
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• 2010

Purpose: Vascular endothelial growth factor (VEGF) is known as a growth factor of endothelium and fibroblast. The purpose is to know the VEGF effects on fibroblast proliferation and fibroblast's notch receptor expression. Methods: CCD-986sk fibroblast was purchased from the Korean Cell Bank and was used in XTT assay for proliferation and wound healing assay for migration. Immunofluorescent (IF) staining and western blotting were used in testing notch expression of fibroblast. Semiquantitative RT-PCR was used in checking notch 1 mRNA production by fibroblast. Student-t test was used for analyzing results. Results: Cell proliferation assay using XTT showed significant higher proliferation in VEGF treated fibroblast, $2.324{\pm}0.0026$ vs. $2.463{\pm}0.017$ (p=0.002). Wound healing assay showed longer migration in VEGF treated fibroblast (p=0.062). The fluorescence was brighter in VEGF treated cells of notch 1 IF staining. Notch 1 expressions and mRNA productions increased more in VEGF treated cells. Conclusion: VEGF stimulates fibroblast to proliferate, migrate and to express Notch 1 simultaneously. Notch receptor could be related to VEGF mediated wound healing.

• International Journal of Railway
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• v.5 no.2
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• pp.89-92
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• 2012

Effective notch stress, as an approach to evaluate the local stress at a notch (weld toe or root), is defined as the total stress assuming linear-elastic material behavior. This method can be effectively used to evaluate the fatigue performance of welded joints. In this study, finite element analysis results using the effective notch stress method were correlated with fatigue test results of rib-to-deck welded joints in a steel orthotropic bridge deck. Effective notch stress approach provided a good correlation with the crack pattern observed in the full-scale fatigue test. A higher effective notch stress at the critical weld toe than at the weld root was consistent with the dominant crack pattern observed at the weld toe during testing. The effective notch stress at the toe on the deck plate was about 80% higher than that on the rib; no cracks at the weld toe on the rib in the testing were observed. Maximum effective notch stress at the weld root occurred on the upper side of the root notch, which indicates that cracks are more likely to propagate into the deck plate, not into the weld metal. This is also consistent with the observed crack pattern in which the crack from the root propagated upward into the deck plate. No such crack pattern, propagating into the weld metal, was observed in the testing.

• Journal of Sensor Science and Technology
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• v.25 no.1
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• pp.8-12
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• 2016

A planar, light-weight, and low-cost notch filter structure is required for the KSTAR ECEI (Electron Cyclotron Emission Imaging) system to protect the mixer arrays from spurious plasma heating power. Without protection, this heating power can significantly degrade or damage the performance of the mixer array. To protect mixer arrays, a frequency selective surface (FSS) structure is the suitable choice as a notch filter to reject the spurious heating power. The FSS notch filter should be located between the lenses of the ECEI system. This paper presents a 170 GHz FSS notch filter for the KSTAR ECEI sensor application. The design of such an FSS notch filter is based on the single-sided square loop geometry, because that makes it relatively insensitive to the incident angle of incoming wave. The FSS notch filter exhibits high notch rejection with low pass-band insertion loss over a wide range of incident angles. This paper also reviews the simulated and measured results. The proposed FSS notch filter might be implemented in other millimeter-wave plasma devices.

• Structural Engineering and Mechanics
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• v.69 no.1
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• pp.51-67
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• 2019

In this paper, the anisotropy of tensile behaviours of layered rocks consisting internal notch has been investigated using particle flow code. For this purpose, firstly calibration of PFC2D was performed using Brazilian tensile strength. Secondly Brazilian test models consisting bedding layer was simulated numerically. Thickness of layers was 10 mm and layered angularity was $90^{\circ}$, $75^{\circ}$, $60^{\circ}$, $45^{\circ}$, $30^{\circ}$, $15^{\circ}$ and $0^{\circ}$. The strength of bedding interface was too high. Each model was consisted of one internal notch. Notch length is 1 cm, 2 cm and 4 cm and notch angularities are $60^{\circ}$, $45^{\circ}$, $30^{\circ}$, $15^{\circ}$ and $0^{\circ}$. Totally, 90 model were tested. The results show that failure pattern was affected by notch orientation and notch length. It's to be noted that layer angle has not any effect on the failure pattern. Also, Brazilian tensile strength is affected by notch orientation and notch length.

• Structural Engineering and Mechanics
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• v.38 no.5
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• pp.619-631
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• 2011

This paper provides a complex variable BIE for solving the periodic notch problems in plane plasticity. There is no limitation for the configuration of notches. For the periodic notch problem, the remainder estimation technique is suggested. In the technique, the influences on the central notch from many neighboring notches are evaluated exactly. The influences on the central notch from many remote notches are approximated by one term with a multiplying factor. This technique provides an effective way to solve the problems of periodic structures. Several numerical examples are presented, and most of them have not been reported previously.

• International Journal of Precision Engineering and Manufacturing
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• v.3 no.3
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• pp.69-75
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• 2002

Measuring dynamic fracture toughness of brittle and small ceramic specimen is very difficult in a SHPB (Split Hopkinson Pressure Bar). As a countermeasure to this difficulty, a dynamic fracture toughness measuring method by the Chevron-notch ceramic specimen was proposed. Tested chevron specimens were of Chevron notch angles of 90$^{\circ}$, 100$^{\circ}$ and 110$^{\circ}$. Through finite element analysis, shape parameters of the Chevron-notch specimens according to notch angles were calculated. And the static fracture tough1ess of the Chevron-notch alumina specimen was measured as 3.8MPa√m similar to that of CT specimen with a precrack. Dynamic fracture toughness was 4.5MPa√m slightly higher than the static one. It was shown in this study that the proposed Chevron-notch specimens are valid to measure dynamic fracture toughness of extremely brittle materials such as ceramic.

• Molecules and Cells
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• v.27 no.1
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• pp.15-19
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• 2009

Notch signaling is controlled at multiple levels. In particular, stabilized Notch receptor activation directly affects the transcriptional activations of Notch target genes. Although some progress has been made in terms of defining the regulatory mechanism that alters Notch stability, it has not been determined whether Notch1/NICD stability is regulated by $GSK-3{\alpha}$. Here, we show that Notch1/NICD levels are significantly regulated by $GSK-3{\beta}$ and by $GSK-3{\alpha}$. Treatment with LiCl (a specific GSK-3 inhibitor) or the overexpression of the kinase-inactive forms of $GSK-3{\alpha}/{\beta}$ significantly increased Notch1/NICD levels. Endogenous NICD levels were also increased by either $GSK-3{\alpha}/{\beta}$- or $GSK-3{\alpha}$-specific siRNA. Furthermore, it was found that $GSK-3{\alpha}$ binds to Notch1. Deletion analysis showed that at least three Thr residues in Notch1 (Thr-1851, 2123, and 2125) are critical for its response to LiCl, which increased not only the transcriptional activity of endogenous NICD but also Hes1 mRNA levels. Taken together, our results indicate that $GSK-3{\alpha}$ is a negative regulator of Notch1/NICD.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.4
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• pp.291-297
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• 2013

Notch1 has been reported to be highly expressed in triple-negative and other subtypes of breast cancer. Mutant p53 (R280K) is overexpressed in MDA-MB-231 triple-negative human breast cancer cells. The present study aimed to determine whether the mutant p53 can be a potent transcriptional activator of the Notch1 in MDA-MB-231 cells, and explore the role of this mutant p53-Notch1 axis in curcumin-induced apoptosis. We found that curcumin treatment resulted in an induction of apoptosis in MDA-MB-231 cells, together with downregulation of Notch1 and its downstream target, Hes1. This reduction in Notch1 expression was determined to be due to the decreased activity of endogenous mutant p53. We confirmed the suppressive effect of curcumin on Notch1 transcription by performing a Notch1 promoter-driven reporter assay and identified a putative p53-binding site in the Notch1 promoter by EMSA and chromatin immunoprecipitation analysis. Overexpression of mutant p53 increased Notch1 promoter activity, whereas knockdown of mutant p53 by small interfering RNA suppressed Notch1 expression, leading to the induction of cellular apoptosis. Moreover, curcumin-induced apoptosis was further enhanced by the knockdown of Notch1 or mutant p53, but it was decreased by the overexpression of active Notch1. Taken together, our results demonstrate, for the first time, that Notch1 is a transcriptional target of mutant p53 in breast cancer cells and suggest that the targeting of mutant p53 and/or Notch1 may be combined with a chemotherapeutic strategy to improve the response of breast cancer cells to curcumin.

• Journal of Microbiology
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• v.44 no.2
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• pp.217-225
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• 2006

Kaposi's sarcoma-associated herpesvirus (KSHV) RTA transcription factor is recruited to its responsive elements through interaction with RBP-Jk that is a downstream transcription factor of the Notch signaling pathway that is important in development and cell fate determination. This suggests that KSHV RTA mimics cellular Notch signal transduction to activate viral lytic gene expression. Here, I demonstrated that unlike other B lymphoma cells, KSHV -infected primary effusion lymphoma BCBL1 cells displayed the constitutive activation of ligand-mediated Notch signal transduction, evidenced by the Jagged ligand expression and the complete proteolytic process of Notch receptor I. In order to investigate the effect of Notch signal transduction on KSHV gene expression, human Notch intracellular (hNIC) domain that constitutively activates RBP-Jk transcription factor activity was expressed in BCBL1 cells, TRExBCBL1-hNIC, in a tetracycline inducible manner. Gene expression profiling showed that like RTA, hNIC robustly induced expression of a number of viral genes including KS immune modulatory gene resulting in downregulation of MHC I and CD54 surface expression. Finally, the genetic analysis of KSHV genome demonstrated that the hNIC-mediated expression of KS during viral latency consequently conferred the downregulation of MHC I and CD54 surface expression. These results indicate that cellular. Notch signal transduction provides a novel expression profiling of KSHV immune deregulatory gene that consequently confers the escape of host immune surveillance during viral latency.