• 대한한의학방제학회지
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• 제22권1호
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• pp.177-192
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• 2014

Objective : In order to investigate the effect of Curcumae Longae Rhizoma(CLR) extract on the hepatocellular carcinogenesis and acute liver damage induced by diethylnitrosamine(DENA) and $CCl_4$ in rats. Methods : Experimental groups were subdivided into four; normal group (Nor), acute liver damage and hepatocellular cancer inducing control group (Con), and CLR extract 200mg/kg/day (CAA) or 400mg/kg/day (CAB) administered groups to Con. Thereafter the changes of the body weight, the liver weight and the weight of liver/100g body weight, total cholesterol, HDL cholesterol, triglyceride, the activities of AST, ALT, ALP, LDH, AFP, SOD, catalase were measured. And we observed by optical and electron microscopy. Result : 1. The body weight was decreased in Con compared with Nor for 5 weeks, but increased in Con compared with Nor from 6 week to 9 week. During experimental period of total 9 weeks, CAA and CAB were increased compared with Con. 2. The liver weight was increased significantly (p<0.05) in Con compared with Nor. The weight of liver/100g body weight was increased significantly (p<0.05) in Con compared with Nor and decreased significantly (p<0.05) in CAB compared with Con. 3. The level of total cholesterol was increased in Con and CAA compared with Nor, but there was not statistically significant. The level of triglyceride was decreased in Con compared with Nor. But increased in CAA and CAB compared with Con. The level of HDL-cholesterol was significantly increased (p<0.05) in CAA and CAB compared with Con. 4. The activities of AST, ALT were increased in Con compared with Nor, but decreased in CAA compared with Con, significantly decreased (p<0.05) in CAB compared with Con. 5. The activities of ALP, LDH were increased in Con compared with Nor, but decreased in CAA and CAB compared with Con. 6. The activities of AFP was increased significantly (p<0.05) in Con compared with Nor, but decreased significantly (p<0.05) in CAA and CAB compared with Con. 7. The activities of SOD were increased in Con, CAA and CAB compared with Nor, but decreased in CAA and CAB compared with Con. The activities of Catalase was more increased in CAA and CAB compared than Con. 8. The results of light microscopical observation, a number of hepatocytes were damaged in Con compared with Nor and CAB. 9. According to the electron microscopical observation, irregular nuclear membrane, condensed nucleoplasm was observed in Con, the experimental group was observed in the nucleus of the well-preserved and evenly developed nucleoplasm. Conclusions : These results suggest that administration of CLR extract suppress or retard on the hepatocellular carcinogenesis and acute liver damage induced by DENA and $CCl_4$ in rats.

• 대한치과기공학회지
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• 제11권1호
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• pp.23-34
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• 1989

The great sudenn change of circle of dental laboratory technology brought mary discord between the new growing environmental group and the old group, In order to know the satisfactory degree of dental laboratory heads about all dental College, This Study was conducted for 49 detal technicians who graduated from junior laboratory heads in Seoul and Kyoung Gi area. The results are abtained as follow- 1. By age The degree of Satisfaction of items about all graduate dental technician were "41$\sim$45 age" group 26.53%), "46$\sim$50 age" group 22.45%, "36$\sim$40 age" group 18.36%, "31$\sim$35 age" group 12.24%, "51$\sim$55 age" group and "56$\sim$60 age" group 8.16% 60$\sim$age group 4.08% in order. 2. By Job Career. The Highest degree item out of Satisfaction about all graduated dental dental technician was 21$\sim$25 years group 28.5%, 16$\sim$20 years 20.4%, 11$\sim$15 years group 12.24%, 26$\sim$20%, 6$\sim$10 years group 4.08%, below 5 years group 2.04% in order. 3. By managing term of respondent's dental laboratory. The Satisfactory degree of items about all gradate technician were 7$\sim$10 years group 36.73% over 15 years group 22.45%, 11$\sim$14 years group 20.41%, 3$\sim$6 years group 14.29% below 2 years group 6.12% in order 4. By dental technician number of respondent's dental laboratory. The satisfactory degree intems about man were as follow; 6$\sim$9 persons group is 42.86 % The Satisfactory degree items about woman were one persons group in 34.69% 5. By born place The dental laboratory heads mean of inters about all dental technician were Seoul group 20.64 %, Kyoung Gi 17.20 %, Jen Nam group 14.99 % Chung Nam 9.5%in order 6. The satisfactory degree of items about all graduate dental technician were neither good nor bad group 32.65%, bad 6.12%, By ability of adaptaton, The satisfactory degree items about man were neither good nor bad group 42.86%, bad group 40.82%. The satisfactory degree of items about woman were neither good nor bad group 40.82% bad group 36.73%. 7. By sincerity. The satisfactory degree of items about man dental technician who graduated from Junior College were neither good nor bad group 52.02%, bad group 4.08, The satisfactory degree about woman dental technician graduated from Junior College were neither good nor bad group 42.86%, bad group 6.12%. 8. By ability of basical of items about man were bad group 40.82 %. The satisfactory degree about woman were 46.94%. 9. By cooperation relation ship. The satisfactory degree of items about man were neither good nor bad group 42.86%, bad group 10.20%. The satisfactory degree about woman were neither good nor bad group 42.86%, bad group 10.20 %. 10. By ability of work. The satisfactory degree of items about man were neither good nor bad group 48.98%, bad group 4.08%. The satisfactory degree about woman were neither good nor bad group 42.86%, bad group 6.12 %. 11. By the curriculum of the dental technician. The satisfactory degree of items about man were neither good nor bad group 67.35%, bad group 2.04%, The satisfactory degree about woman were neither good nor bad group 61.22%, bad group 6.12%. 12. By occupational satisfaction. The satisfactory degree of items about all graduate dental technician were fatisfied group 14.29%. By the out look of employment. The satisfactory degree of items about man greduate dental technician were neither good nor bad group 34.69 %, bad group 10.20%, about woman graduate dental technician were neither good nor bad group 44.90%, bad group 10.20%, Their employment prospect is not so bright or oprimistic due to the glut supply by graduates in the job-seeking market 13. The satisfactory rate on the artificial dental manufacturing world in terms of effects produced by juror College graduate dental technicians shared good group 40.82% bad group 2.04% 14. By cognition of the number of graduate dental technician. The satisfactory degree of dental laboratory heads about man graduate dental technician were too many group 38.78%, few group 6.12% about woman graduated dental technician were neither good nor bad group 30.6% few group 10,20%, The employment opportunities for the artifical dental technicians are getting decreased because of their excessive cumber in supply 15. The opinion regarding the initial salary. ranging from \160,000 to \200,000 for the junior college graduate dental technicians shared 57.14%. The top ratio, while the satisfaction reate on the academic study period of "3-year" group shared 22%, also the top ratio. 16. The improvement policy and prblematic issues presonted by the owner and operatore of atriticial dental manufacturing plants are as shown below First; The viewpoint that they contribute to the improvement of people's oral health in terms of their mission as artificial dental technicians. Second: The cultivation of basic technical ability to cope with clinical practice upon graduation Third; They require guidance in study and research in their filed of profession, that tray may beable to estaclish a from theory.

• Natural Product Sciences
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• 제26권4호
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• pp.283-288
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• 2020

Microbial transformation of a potent progestin, norethisterone (17��-hydroxy-19-nor-17α-pregn-4-en-20-yn-3-one) (1) was carried out by using two filamentous fungi Aspergillus niger and Penicillium citrinum. Biotransformation of 1 with A. niger yielded a hydroxylated transformed product 10��,17��-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (2) whereas 11��,17��-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (3) was obtained through microbial transformation of 1 by P. citrinum. It is the first report of their production from 1 by using A. niger and P. citrinum with complete 1H- and 13C-NMR assignment. The structures of both metabolites were characterized by various spectroscopic techniques and reported data.

• Journal of Animal Science and Technology
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• 제45권5호
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• pp.695-702
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• 2003

Nucleolus Organizer Regions(NORs)는 핵인을 형성하는 염색체의 특정 부위로서 rRNA 합성에 관여하는 리보좀 유전자를 함유하고 있으며 이의 활성화가 일어나는 곳이다. 본 연구에서는 한우의 NORs의 양상을 제시하기 위하여 AgNOR 염색법을 이용하여 한우의 NORs 수와 NORs 염색체 및 이의 분포 위치 등을 구명하고 한편으로 소의 품종별, 성별 및 근원이 다른 세포간의 NORs 양상을 비교 분석하였다. 본 분석에 이용된 공시축은 한우 및 홀스타인 암수 44두로서 이들의 혈액배양으로부터 염색체를 분리하였다. 조직간 세포의 NORs 비교를 위하여 귀 조직으로부터 배양된 섬유아세포의 염색체와 백혈구 배양으로부터 분리된 염색체를 분석하였다. 분리된 중기상에 AgNOR 염색 후 G-banding을 한 결과 한우의 NORs는 2번, 3번, 4번, 11번 및 28번 염색체에 존재하고, 이들의 분포 위치는 각 염색체의 말단부에 위치한다. 한우 NORs 수는 세포에 따라 최소 2개에서부터 최대 10개까지 나타나며 평균 5.6개였다. 이러한 다형적 양상은 개체 간뿐만 아니라 동일 개체 내 세포 간에서도 달리 나타나며 발현의 크기 또한 차이가 있다. 품종 간 NORs의 비교 분석에서 한우의 NORs 수가 Holstein (5.4개)에 비해 유의적으로 높게 나타났으며, 근원이 다른 세포간 비교에서는 fibroblasts (5.9개)에서의 NORs 수가 lymphocytes (5.5개)에 비해 높은 빈도를 보였고, 성 간에는 수컷 (5.7개)이 암컷 (5.4개)에 비하여 많은 NORs 수를 나타내었다. 그러나 이들의 염색체상 분포 양상에서는 공히 동일한 염색체에 출현되었으며 염색체 상 출현 빈도에서도 세포들 간에 거의 차이가 없었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8155-8161
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• 2016

The prognostic significance of AgNOR proteins in stage II-III rectal cancers treated with chemoradiotherapy was evaluated. Silver staining was applied to the $3{\mu}m$ sections of parafin blocked tissues from 30 rectal cancer patients who received 5-FU based chemoradiotherapy from May 2003 to June 2006. The microscopic displays of the cells were transferred into the computer via a video camera. AgNOR area (nucleolus organizer region area) and nucleus area values were determined as a nucleolus organizer regions area/total nucleus area (NORa/TNa). The mean NORa/TNa value was found to be $9.02{\pm}3.68$. The overall survival and disease free survival in the high NORa/TNa (>9.02) patients were 52.2 months and 39.4 months respectively, as compared to 100.7 months and 98.4 months in the low NORa/TNa (<9.02) cases. (p<0.001 and p<0.001 respectively). In addition, the prognosis in the high NORa/TNa patients was worse than low NORa/TNa patients (p<0.05). In terms of overall survival and disease-free survival, a statistically significant negative correlation was found with the value of NORa/TNa in the correlations tests. Cox regression analyses demostrated that overall survival and disease-free survival were associated with lymph node status (negative or positive) and the NORa/TNa value. We suggest that two-dimensional AgNOR evaluation may be a safe and usable parameter for prognosis and an indicator of cell proliferation instead of AgNOR dots.

• Biomolecules & Therapeutics
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• 제4권3호
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• pp.251-256
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• 1996

The metabolism of carbinoxamine, 2-[(4-chlorophenyl)-2-pyridinyl-methoxy]-N, N-dimethylethaneamine, was studied in adult male volunteers after an oral dose of 15 mg. Solvent extracts of urine obtained with or without enzyme hydrolysis were analyzed by gas chromatography-mass spectrometry after derivatization with MSTFA/TMSCl (N-methyl-N-trimethylsilyltrifluoroacetamide/trimethyl chlorosilane). The structures of metabolites were determined based on the electron impact (EI) and chemical ionization (CI) mass spectra. Nonconjugated metabolites identified in the urine were carbinoxamine, nor-carbinoxamine, and bits-nor-carbinoxamine. Parent drug, nor-carbinoxamine, and bits-nor-carbinoxamine were also detected as conjugated forms. These metabolites observed in human urine were different from those previously reported in the rat. Urinary excretions of carbinoxamine were reached to maxima in 4 hours after drug administration with 4.9%-8.1% and 2.5-4.2% of the dose excreted during 24 h as carbinoxamine and its glucuronide, respectively.

• Natural Product Sciences
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• 제29권1호
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• pp.17-23
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• 2023

A phytochemical investigation of the fruits extract of Melia toosendan afforded the isolation of two new nor-neolignans, meliasendanins E (1) and F (2), as well as twelve known compounds (3 - 14) using various separation technique such as Diaion HP20, silica, RP-18 gel column chromatography and semi-preparative HPLC. Their chemical structures were elucidated by extensive NMR spectroscopic data including 2D-NMR, and HR-ESI-MS as well as ECD data. Among the twelve known compounds, the absolute structures of 3 - 6 were determined first, and given the trivial names as meliasendanins G-J (3 - 6). Based on the evaluation of anti-inflammatory activity, compounds 7 - 8 exhibited inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages with IC₅₀ values of 34.6 and 39.5 µM, respectively.

• 생명과학회지
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• 제12권5호
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• pp.605-609
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• 2002

개의 염색체수는 2n=78이며 염색체의 형태를 보면 submetacentric chromosome metacentric chromosome인 X와 Y를 제외하고 모든 상염색체가 acrocentric chromosome이며 그 크기가 작고 크기의 차이가 나지 않는 염색체들이 많아 G-banding등에 의한 핵형의 표준화가 완전하게 이루어지고 있지 않다. 본 연구fl서는 G-banding high-resolution-banding NOR- banding등의 방법을 이용하여 개의 미소 염색체들에 대한 핵형 및 염색체 동정을 하고자 하였다. C-banding과 high-resolution banding에 의한 그 결과 아직 표준화가 되어 있지 않은 염색체 중 34번, 37번 등의 염색체에서 본 연구 결과가 차이가 있었으나 이는 해석상의 차이로 보이며 NOR-banding에 의한 분석결과 Y염색체와 3쌍의 상염색체에서 존재함을 확인 할 수 있었고 이는 개의 염색체 동정의 지표로 활용할 수 있으리라고 생각된다.

• Molecules and Cells
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• 제22권1호
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• pp.8-12
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• 2006

Neuron-derived orphan receptor (NOR-1) is a member of the thyroid/steroid receptor superfamily that was originally identified in forebrain neuronal cells undergoing apoptosis. In addition to apoptotic stimuli, activation of several signal transduction pathways including direct neuronal depolarization regulates the expression of NOR-1. In this study we tested whether the expression of NOR-1 is changed following transient ischemic injury in the adult rat brain. NOR-1 mRNA increased rapidly in the dentate gyrus of the hippocampal formation and piriform cortex 3 h after transient global ischemia and returned to basal level at 6 h. On the other hand, oxygen-glucose deprivation of cultured cerebral cortical neurons did not alter the expression of NOR-1. These results suggest that expression of NOR-1 is differentially regulated in different brain regions in response to globally applied brain ischemia, but that hypoxia is not sufficient to induce its expression.

• 한국산학기술학회논문지
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• 제9권3호
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• pp.710-716
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• 2008

본 논문은 플래시 메모리를 이용한 파일 저장 장치의 성능 분석을 다룬다. 특히 플래시 메모리의 대표적인 형태인 낸드(NAND) 플래시와 노어(NOR) 플래시에 대해 비교 분석한다. 성능 평가를 위해 마이크로소프트 PocketPC 기반의 실험 플랫폼에 두 플래시 타입을 위한 파일 저장 시스템을 각각 구성하였다. 이렇게 구성된 플랫폼을 이용하여, 버퍼 크기, 사용 용량, 커널 수준 쓰기 캐싱 등의 변수에 따른 입출력 처리량을 측정/비교하였다. 실험 결과에 따르면, 낸드 플래시 기반 저장 장치의 성능이 쓰기/읽기 처리량 관점에서 각각 4.8배, 5.7배까지 더 높은 것으로 나타났다. 본 실험 결과는 두 가지 플래시 메모리 저장 방식의 상대적인 장단점을 잘 보여주고 있으며 플래시 메모리 기반의 파일 저장장치의 설계에 유용하게 활용될 수 있다.