Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Identification of Nucleolus Organizer Regions of Korean Cattle Chromosomes by AgNOR Staining

AgNOR 염색법에 의한 한우 염색체의 Nucleolus Organizer Regions 양상 분석

  • Jung , W. (Department of Animal Science and Biotechnology & RAIRC， Jinju National University) ;
  • Sohn, S.H. (Department of Animal Science and Biotechnology & RAIRC， Jinju National University)
  • 정원 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터) ;
  • 손시환 (진주산업대학교 동물생명과학과 . 동물생명산업지역협력연구센터)
  • Published : 2003.10.31
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Abstract

Nucleolus Organizer Regions (NORs) are the specific chromosome sites where ribosomal genes are located and highly expressed. We have applied the AgNOR staining to identify the distribution of NORs in the chromosomes of Korean Cattle. We have also studied the NORs pattern on the cells originated from different breeds, tissues and sex. Peripheral blood from forty-four Korean Cattle and Holstein was cultured for chromosme preparation. The fibroblast culture from biopsied ear skins was also conducted for chromosome analysis. The distribution of NORs was analyzed by sequential Ag staining and G-banding on metaphases of the cells. In Korean Cattle, the NORs are localized on the telomeres of the five chromosome pairs number 2, 3, 4, 11 and 28. The number of NORs per metaphase ranged from 2 to 10 giving a mean value of 5.6. The number of NORs per cell varied among individuals and cells within same individual. The size of NORs also differed in NO-chromosomes. The number of NORs was significantly different between Korean Cattle and Holstein, fibroblasts and lymphocytes, and male and female. However, the distribution and frequency of NORs were similar among the cells regardless of breeds, tissues, and sex.

Nucleolus Organizer Regions(NORs)는 핵인을 형성하는 염색체의 특정 부위로서 rRNA 합성에 관여하는 리보좀 유전자를 함유하고 있으며 이의 활성화가 일어나는 곳이다. 본 연구에서는 한우의 NORs의 양상을 제시하기 위하여 AgNOR 염색법을 이용하여 한우의 NORs 수와 NORs 염색체 및 이의 분포 위치 등을 구명하고 한편으로 소의 품종별, 성별 및 근원이 다른 세포간의 NORs 양상을 비교 분석하였다. 본 분석에 이용된 공시축은 한우 및 홀스타인 암수 44두로서 이들의 혈액배양으로부터 염색체를 분리하였다. 조직간 세포의 NORs 비교를 위하여 귀 조직으로부터 배양된 섬유아세포의 염색체와 백혈구 배양으로부터 분리된 염색체를 분석하였다. 분리된 중기상에 AgNOR 염색 후 G-banding을 한 결과 한우의 NORs는 2번, 3번, 4번, 11번 및 28번 염색체에 존재하고, 이들의 분포 위치는 각 염색체의 말단부에 위치한다. 한우 NORs 수는 세포에 따라 최소 2개에서부터 최대 10개까지 나타나며 평균 5.6개였다. 이러한 다형적 양상은 개체 간뿐만 아니라 동일 개체 내 세포 간에서도 달리 나타나며 발현의 크기 또한 차이가 있다. 품종 간 NORs의 비교 분석에서 한우의 NORs 수가 Holstein (5.4개)에 비해 유의적으로 높게 나타났으며, 근원이 다른 세포간 비교에서는 fibroblasts (5.9개)에서의 NORs 수가 lymphocytes (5.5개)에 비해 높은 빈도를 보였고, 성 간에는 수컷 (5.7개)이 암컷 (5.4개)에 비하여 많은 NORs 수를 나타내었다. 그러나 이들의 염색체상 분포 양상에서는 공히 동일한 염색체에 출현되었으며 염색체 상 출현 빈도에서도 세포들 간에 거의 차이가 없었다.

Keywords

References

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