• 한국멀티미디어학회논문지
• /
• 제1권2호
• /
• pp.162-172
• /
• 1998

본 논문에서는 영문 텍스트 검색 엔진인 MG(Managing Gigabytes) 시스템과 한글 형태소 분석기 HAM (Hangul Analysis Module)을 이용하여 기가바이트 크기의 텍스트 데이타 처리가 가능한 한글 텍스트 검색 엔진 HMG(Hangul MG)를 구현하였다. 한글 처리를 위해 KSC 5601 완성형 코드를 사용하여 데이타베이스 구축 단계와 질의 처리 단계에서 사용하였다. HMG의 개발을 위해 MG 시스템의 렉시칼 분석기와 파서, 인텍스 구성 모률을 수정하였다. HMG 시스템의 유용성을 보이기 위해 웹에서 한글 소설을 검색할 수 있도록 하는 N NOD (Novel On Demand) 시스템올 구현하였다. HMG 시스템은 한글이 포함된 대규모 전문 검색 시스템의 구축에 활용될 수 있다.

• 농업과학연구
• /
• 제48권2호
• /
• pp.251-264
• /
• 2021

Bovine respiratory syncytial virus (BRSV) and bovine viral diarrhea virus (BVDV) are the main viral contributors to bovine respiratory disease (BRD) with high mortality and morbidity. BRD control measures include vaccination that modulates immunological profiles reflected in blood cells, serum, and body secretions, such as milk. This study evaluated the immune responses to an inactivated BRD vaccine in lactating cows reared in a natural environment on a dairy farm. The cows were intramuscularly inoculated with the vaccine, and serum, blood, and milk were collected pre-and post-vaccination. Our study revealed a prominent increase in BRSV-specific antibodies both in serum and milk, while the change in BVDV-specific antibodies was insignificant. Serum interleukin (IL)-1β and IL-6 levels significantly decreased, but this change was not reflected in milk. Evaluation of pattern recognition receptors (PRRs) via RT-qPCR revealed downregulation of nucleotide-binding oligomerization domain 2 (NOD2). The concentrations of BRSV antibodies, BVDV antibodies, IL-2, and IL-17A in serum and milk were strongly correlated, implying a concurrent influence on both body fluids. Thus, immunological factors modulated as a result of vaccination generally measured in serum were reflected in milk, demonstrating the suitability of milk evaluation as an alternative approach for immunological observations. Furthermore, the correlation between BRSV antibodies and NOD2 and that between BVDV antibodies and toll-like receptor (TLR) 2, TLR3, TLR4, and TLR5 imply the possible role of PRRs for the assessment of the immune response developed in immunized cows reared on the farm.

• 대한본초학회지
• /
• 제26권2호
• /
• pp.1-10
• /
• 2011

Objective ： Lonicera japonica contains anti complementary polysaccharides and polyphenolic compound. Among these polyphenolic substances, chlorogenic acid is the major active component of this plant. However, the immunological mechanisms for these activities, have not been elucidated, nor the active components. To clarify immunomodulatory effects of those we examined the relationship between the activity of CD8+ T cell-mediated lysis and the frequency of cytokine profiles in spleen, thymus (especially IFN-${\gamma}$, IL-4, GM-CSF etc.) expressing CD8+ T cells activated by IL-2. Methods : To study immunomodulatory effects ethyl acetate fraction from Lonicera japonica, chlorogenic acid on cytokine gene expression from spleen, thymus cells, RT-PCR was performed after quantitative normalization for each gene by a densitometry using ${\beta}$-actin gene expression. A modified standard $^{51}Cr$-release assay was used to measure cytotoxic activities of cytotoxic T cells. Spleen, thymus cells from NOD mice were stained with CD3, CD4, CD44, CD69 in staining buffer and analyzed by two color flow cytometry. Results : We showed that ethyl acetate fraction from Lonicera japonica in combination with IL-2 resulted in a significant enhancement of PCR products for IFN-${\gamma}$, IL-4, IL-10, GM-CSF, IL-6 and cytotoxtic CD8+ T cell proportion in spleen and thymus T cells in NOD mice. This suggests that IFN-${\gamma}$, IL-6 like IL-4 may be acting as a regulatory rather than proinflammatory cytokine. Conclusions : In conclusion, based on the results of the present study which showed that ethyl acetate fraction from Lonicera japonica and chlorogenic acid upregulating cytokine gene expression in spleen and thymus, we are tempted to speculate that some of the therapeutic efficacies such as anti-diabetic activity of Lonicera japonica are due to the immunomodulatory its ethylacetate fraction and chlorogenic acid.

• Journal of applied mathematics & informatics
• /
• 제32권1_2호
• /
• pp.161-169
• /
• 2014

We in this paper obtained the theoretical results for multivariate stochastic processes which help us to extended negatively orthant dependent(ENOD) structures among hitting times of the processes. In addition, some applications are given to illustrate these concepts.

• 대한본초학회지
• /
• 제30권2호
• /
• pp.1-9
• /
• 2015

Objectives : The present study was conducted to examine whether Toosendan Fructus has an ameliorative effect on diabetes-induced alterations such as oxidative stress and inflammation in the pancreas of non-obese diabetic (NOD) mice, a model of human type I diabetes. Methods : Extracts of Toosendan Fructus (ETF) were administered to NOD mice at three doses (50 mg/kg, 100 mg/kg and 200 mg/kg). Mice at 18 weeks of age were measured glucose tolerance using intraperitoneal glucose tolerance test. After 28 weeks of ETF treatment, glucose, total cholesterol (TC), triglyceride (TG), and proinflammatory cytokines in serum, western blot analyses and a histopathological examination in pancreas tissue, and on the onset of diabetes were investigated. Results : The results showed that levels of glucose, glucose tolerance, TC, TG, interferon-${\gamma}$, interleukin (IL)-1 ${\beta}$, IL-6, and IL-12 in serum were down-regulated, while IL-4, IL-10, SOD, and catalase significantly increased. In addition, ETF improved protein expression of proinflammatory mediaters (such as cyclooxygenase-2, and inducible nitric oxide synthase) and a proapoptotic protein (caspase-3) in the pancreatic tissue. Also, in the groups treated with ETF (100 mg/kg or 200 mg/kg), insulitis and infiltration of granulocytes were alleviated. Conclusions : Based on these results, the anti-diabetic effect of ETF may be due to its anti-inflammatory and antioxidant effect. Our findings support the therapeutic evidence for Toosendan Fructus ameliorating the development of diabetic pancreatic damage via regulating inflammation and apoptosis. Our future studies will be focused on the search for active compounds in these extracts.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
• /
• 제16권5호
• /
• pp.513-518
• /
• 2010

정보기술은 통신 및 멀티미디어 기술의 발전에 힘입어 빠르게 발전하고 있으며, 이에 따른 디지털 콘텐츠에 대한 수요가 증가하였다. 디지털 콘텐츠 중 비디오 콘텐츠는 VOD, NOD, 디지털 도서관, IPTV, UCC 등 다양한 응용 분야에서 점점 확산되고 있다. 비디오 데이터는 순차적인 특성이 있으며, 시간과 공간정보가 결합한 3차원 데이터로서 계산 비용이 많이 드는 작업이기 때문에 검색이나 브라우징이 어렵다. 본 논문에서는 비디오 데이터를 구조화하는 동영상 내용 편집 도구(ATVC: Authoring Tool for Video Contents)를 제안한다. 동영상 내용 편집 도구는 비주얼 리듬을 이용하여 비디오 데이터에서 대표 프레임을 추출한 다음 XML을 이용한 태그 및 키워드 정보를 대표 프레임에 삽입하는 도구(Tool)이다. 비주얼 리듬은 3차원의 시공간적인 정보를 2차원으로 매핑한 정보로 IDCT과정 없이 픽셀 정보를 얻을 수 있어 처리속도가 빠르며 컷, 와이프, 디졸브 등의 편집 효과를 효과적으로 구분할 수 있다. 그리고 XML 데이터에는 태그 및 키워드 정보와 함께 대표 프레임의 정보까지 저장되므로 효율적인 브라우징을 제공할 수 있다.

• Journal of Oral Medicine and Pain
• /
• 제45권4호
• /
• pp.89-96
• /
• 2020

Purpose: To investigate the expression of malondialdehyde (MDA), lipid peroxidation marker for oxidative stress (OS), in autoimmune Sjögren's syndrome (SjS) by utilizing the SjS-prone C57BL/6.NOD-Aec1Aec2 (B6DC) mouse and the SjS patient plasma samples. Methods: The MDA concentrations in the lysates of the submandibular gland, liver, and serum samples from the SjS-prone B6DC mouse model were compared with those from the C57BL/6J as a control. A thiobarbituric acid reactive substance (TBARS) assay kit was used to measure MDA. Plasma samples from five SjS patients and five control subjects were also evaluated. Results: The MDA concentrations in experimental animals and controls were not significantly different. There were no significant differences between the plasma of SjS patients and of controls. Conclusions: The expression of MDA was investigated in the organs from the SjS-prone B6DC mouse for the first time and in the plasma samples of SjS patients. No significant differences were observed between SjS and control samples when MDA was the target molecule with the TBARS assay. MDA may not be a reliable marker to measure OS contrary to the published studies involving OS of SjS.

• Journal of Ginseng Research
• /
• 제44권2호
• /
• pp.300-307
• /
• 2020

Background: Emerging evidence suggests that endothelial-to-mesenchymal transition (EndMT) in endothelial dysfunction due to persistent inflammation is a key component and emerging concept in the pathogenesis of vascular diseases. Ginsenoside Rg3 (Rg3), an active compound from red ginseng, has been known to be important for vascular homeostasis. However, the effect of Rg3 on inflammation-induced EndMT has never been reported. Here, we hypothesize that Rg3 might reverse the inflammation-induced EndMT and serve as a novel therapeutic strategy for vascular diseases. Methods: EndMT was examined under an inflammatory condition mediated by the NOD1 agonist, γ-d-glutamyl-meso-diaminopimelic acid (iE-DAP), treatment in human umbilical vein endothelial cells. The expression of EndMT markers was determined by Western blot analysis, real-time polymerase chain reaction, and immunocytochemistry. The underlying mechanisms of Rg3-mediated EndMT regulation were investigated by modulating the microRNA expression. Results: The NOD1 agonist, iE-DAP, led to a fibroblast-like morphology change with a decrease in the expression of endothelial markers and an increase in the expression of the mesenchymal marker, namely EndMT. On the other hand, Rg3 markedly attenuated the iE-DAP-induced EndMT and preserved the endothelial phenotype. Mechanically, miR-139 was downregulated in cells with iE-DAP-induced EndMT and partly reversed in response to Rg3 via the regulation of NF-κB signaling, suggesting that the Rg3-miR-139-5p-NF-κB axis is a key mediator in iE-DAP-induced EndMT. Conclusion: These results suggest, for the first time, that Rg3 can be used to inhibit inflammation-induced EndMT and may be a novel therapeutic option against EndMT-associated vascular diseases.

• 한국정보처리학회논문지
• /
• 제6권5호
• /
• pp.1410-1417
• /
• 1999

In this paper, we propose the method of extracting the caption regions from news video and the method of recognizing the captions that can be used mainly for content-based indexing and retrieving the MPEG-2 compressed news for NOD(News On Demand). The proposed method can reduce the searching time on detecting caption frames with minimum MPEG-2 decoding, and effectively eliminate the noise in caption regions by deliberately devised preprocessing. Because the kind of fonts that are used for captions is not various in the news video, an enhanced template matching method is used for recognizing characters. We could obtain good recognition result in the experiment of sports news video by the proposed methods.

• 한국토양비료학회지
• /
• 제27권2호
• /
• pp.136-146
• /
• 1994

대두 근류균 공생에서 뿌리분비물에 대한 근류균의 화학주성이 근류형성에 미치는 영향을 조사 연구한 결과는 다음과 같다. 2mM proline에 대한 표준균주와 변이주의 화학주성비는 KCTC 2422는 3.1, LPN-100은 1.3, LCR-101은 1.0이었으며 전형적인 Slow growing Rhizobia였다. 팔달 및 백운의 뿌리분비물 추출액에 대한 근류균의 화학주성비는 KCTC 2422는 3.5/3.2 이고 LPN-100은 1.3/1.4이며 LCR-101은 1.4/1.4정도였다. Dowex 칼럼으로부터 용출시킨 Fraction I, II, III에 대한 화학주성비는 Fraction I>II>III순이었고 중성분획인 Glucose와 Ribose, 양성분획인 Aspartic acid와 Glutamine, 음성분획인 Citric acid, Succinic acid에 대한 화학주성비는 공히 $10^{-3}M$일때 가장높은 화학주성비를 나타내었다. 근류형성의 정도는 KCTC 2422는 접종후 7일경부터 Nitrogenase 활성이 나타나기 시작하며 LCR-101은 15일 이후에 Nitrogenase 활성이 나타났으며 LPN-100은 전혀 근류를 형성하지 않아 근류균의 화학주성이 숙주인식의 초기과정에 영향을 미쳤음을 확인할 수 있었다.