• Journal of Korean Society of Environmental Engineers
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• v.35 no.4
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• pp.294-300
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• 2013

In this study, major parameter of partial nitritation was investigated for the stable operation. In order to establish partial nitritation system, prevailing parameters such as temperature, BA (bicarbonate alkalinity) and pH were evaluated. As a result, it is inferred that appropriate bicarbonate alkalinity ratio (mg $NaHCO_3{\cdot}L^{-1}/mg$ Inf. $NH_4{^+}-N{\cdot}L^{-1}$) drives stable 50% partial nitritation at $32^{\circ}C$ and ambient temperature, respectively. Alkalinity ratio was proposed as new strategy for 50% partial nitritation without pH control in both temperature regimes. Because of the results, it was added amound of BA required only for 50% nitritation to inhibit nitratation. The effluent $NO_2{^-}-N/NH_4{^+}-N$ ratio reached almost 100% when initial bicarbonate alkalinity ratios (mg $NaHCO_3{\cdot}L^{-1}/mg$ Inf. $NH_4{^+}-N{\cdot}L^{-1}$) were 6.8 (R1) and 6.7 (R2), respectively. Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) results demonstrated that AOB was the dominant nitrifying bacteria and NOB was negligible after adopting process control.

• Journal of Korean Society for Atmospheric Environment
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• v.14 no.3
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• pp.229-236
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• 1998

Visibility is a good indicator of comprehensive alto quality. The prevailing visibility in Seoul is no better than the past although the average concentration of SO2 and TSP (total suspended particulate) has decreased quite significantly in the past decade, owing to the government efforts to reduce the emission. Then, a question arises why visibility has not been improved. In order to answer this question we have investigated which components of air pollutants are most responsible for the visibility degradation in Seoul. Analysis on the visibility vs the aerosol sixte distribution measured by an optical particle counter shows that the particles of the size interval between 0.5mm and 2.5mm are most responsible for the visibility degradation. Among the criteria air Pollutants, NOB concentration is found to be moot responsible, rather than PM10. ton analysis of the particulate collected by a high volume sampler shows that the nitrate component increases more significantly than other ions when visibility becomes very poor. Therefore, we conclude that the major causes of visibility degradation in Seoul are the increase of NOB, nitrates and the particles of the size range between 0.5mm and 2.5mm.

• Journal of Korean Society on Water Environment
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• v.38 no.4
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• pp.171-176
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• 2022

Recently, an autotrophic single-stage nitrogen removal (ASSNR) process based on the anaerobic ammonium oxidation (ANAMMOX) reaction has been proven as an economical ammonia treatment. It is highly evident that double-layered gel beads are a promising alternative to the natural biofilm for ASSNR because of the high mechanical strength of poly(vinyl alcohol) (PVA)/alginate structure and efficient protection of ANAMMOX bacteria from dissolved oxygen (DO) due to the thick outer layer. However, the thick outer layer results in severe mass transport limitation and consequent lowered bacterial activity. Therefore, the effects of the thickness of the outer layer on the overall reaction rate were tested in the biofilm model using AQUASIM for ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and ANAMMOX bacteria. A thickness of 0.5~1.0 mm is preferred for the maximum total nitrogen (TN) removal. In addition, a DO of 0.5 mg/L resulted in the best total nitrogen removal. A higher DO induces NOB activity and consequent lower TN removal efficiency. The optimal density of AO B and NO B density was 1~10% for a 10% ANAMMOX bacterial in the double-layered PVA/alginate gel beads. The real effects of operating parameters of the thickness of the outer layer, DO and concentrations of biomass balance should be intensively investigated in the controlled experiments in batch and continuous modes.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1995.11a
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• pp.190-193
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• 1995

MCFC has been investigated for the last half centry as an alternative energy source. The operating temperature of MCFC is 650$^{\circ}C$, so it is more efficient than ocher fuel cells, and it has needed no nob metal element for the electrodes. we investigated electrochemical characteristics of separator for MCFC.

• Korean Journal of Microbiology
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• v.44 no.1
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• pp.29-36
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• 2008

Nitrogen is one of the major pollutants that should be removed by wastewater treatment systems. Biological nitrogen removal (BNR) is a key technology in advanced wastewater treatment systems operated by bacterial populations. Nitrification is the first step of microbiological processes in BNR system. Ammonia is oxidized to nitrite by ammonia-oxidizing bacteria (AOB) and then nitrite is subsequently oxidized to nitrate by nitrite-oxidizing bacteria (NOB). The diversity of NOB in nitrification reactors of 3 BNR systems, Edited biological aerated filter system, Nutrient removal laboratory system, and the Rumination type sequencing batch reactor system, was investigated by terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes. Cluster analysis of T-RF profiles showed that communities of Nitrobacter group in each system were different depending upon the process of systems. However, the clusters of Nitrospira group were divided by the habitat of aqueous and solid samples.

• Journal of Microbiology and Biotechnology
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• v.28 no.7
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• pp.1168-1177
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• 2018

The aim of this study was to investigate the microbial community of three tannery wastewater treatment plants (WWTPs) involved in nitrification by Illumina MiSeq sequencing. The results showed that highly diverse communities were present in tannery wastewater. A total of six phyla, including Proteobacteria (37-41%), Bacteroidetes (6.04-16.80), Planctomycetes (3.65-16.55), Chloroflexi (2.51-11.48), Actinobacteria (1.91-9.21), and Acidobacteria (3.04-6.20), were identified as the main phyla, and Proteobacteria dominated in all the samples. Within Proteobacteria, Beta-proteobacteria was the most abundant class, with the sequence percentages ranging from 9.66% to 17.44%. Analysis of the community at the genus level suggested that Thauera, Gp4, Ignavibacterium, Phycisphaera, and Arenimonas were the core genera shared by at least two tannery WWTPs. A detailed analysis of the abundance of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) indicated that Nitrosospira, Nitrosomonas, and Nitrospira were the main AOB and NOB in tannery wastewater, respectively, which exhibited relatively high abundance in all samples. In addition, real-time quantitative PCR was conducted to validate the results by quantifying the abundance of the AOB and total bacteria, and similar results were obtained. Overall, the results presented in this study may provide new insights into our understanding of key microorganisms and the entire community of tannery wastewater and contribute to improving the nitrogen removal efficiency.

• Journal of Korean Society on Water Environment
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• v.23 no.6
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• pp.920-926
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• 2007

The upflow Biobead$^{(R)}$ process, one of biological aerated filters (BAF), which was used commercially, invented for removal of organic materials and nitrification. This process was modified to enhance the ability of denitrification through the induction of pre-anoxic tank. In this research, we investigated the effects of hydraulic retention time (HRT) and backwashing period in aerobic tank. The characteristics of nitrifying bacteria, which are composed of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), also investigated using fluorescence in situ hybridization (FISH). Even though the HRT was shortened, the efficiency of nitrification was not decreased when the organic loading rate and ammonium-nitrogen loading rate were $2.10kg/m^3/day$ and $0.25kg/m^3/day$, respectively. And then the distribution ratios of AOB and NOB showed the similar patterns. However, when the backwashing period was lengthened from 12 hours to 24 hours in aerobic 1 tank, the nitrification efficiency was decreased to 63.9% from 89.2%. The results of FISH explained that this decrease of nitrification efficiency was caused by the decrease of distribution ratio of AOB in aerobic 1 tank. The nitrification efficiencies of aerobic 1 and aerobic 2 tank were increased when the backwashing period was lengthened because of relative high distribution ratios of nitrifying bacteria.

• Animal cells and systems
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• v.2 no.1
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• pp.123-131
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• 1998

The unc-29 region on the chromosome I of Caenorhabditis elegans has been mutagenized in order to obtain lethal mutations. In this screen, the uncoordinated phenotype of unc-29 (e193) mutant was used to identify any lethal mutations closely linked to the unc-29 gene, which encodes a subunit of nicotinic acetylcholine receptors. We have isolated six independent mutations (jh1 to jh6) out of approximately 5,200 ethyl methanesulfonate(EMS) treated haploids. Four of the six mutations demonstrated embryonic lethal phenotypes, while the other two showed embryonic and larval lethal phenotypes. Terminal phenotypes observed in two mutations (jh1 and jh2) indicated developmental defects specific to posterior part of embryos which appeared similar to the phenotypes observed in nob (no back end) mutants. Another mutation (jh4) resulted in an interesting phenotype of body-wall muscle degeneration at larval stage. These mutations were mapped by using three-factor crosses and deficiency mutants in this region. Here we report genetic analysis and characterization of these lethal mutations.

• Archives of Pharmacal Research
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• v.24 no.5
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• pp.367-370
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• 2001

This study describes the synthesis and in vitro evaluation of noble 2-[3-(cyclopentyloxy)-4-methoxyphenyl]-1-isoindolinone Derivatives for the inhibition of TNF-$\alpa$ production. Among these compounds, 2- [3- (Cyclopentyloxy)- 4- methoxyphenyll- 3- methyl -1 - isoindolinone (5) was the most potent in inhibitory activity of TNF-$\alpa$ production in LPS-stimulated RAW264.7 cells.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.28 no.11
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• pp.1440-1448
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• 2004

Multiple solutions in natural convection of air (Pr=0.7) between two horizontal walls with mean temperature difference and the same periodic nob-uniformities are investigated. An analytical solution is found for small Rayleigh number, and the general solution is investigated by using a numerical method. In the conduction-dominated regime, two upright cells are formed between two walls over one wave length. When the wave number is small, the flow becomes unstable with increase of the Rayleigh number, and multicellular convection occurs above a critical Rayleigh number. The multicellular flows at high Rayleigh numbers consist of approximately square-shape cells. And several kinds of multiple flows classified by the number of cells are found.