Effects of Nitrifying Bacterial Communities with Different HRTs and Backwashing Periods in Modified BAF Process

Modified BAF 공정에서 HRT 및 역세주기가 질산화 미생물의 군집에 미치는 영향

  • Jung, Chul-Soo (Department of Environmental Engineering, Pusan National University) ;
  • Park, Jeung-Jin (Department of Environmental Engineering, Pusan National University) ;
  • Ju, Dong-Jin (Department of Environmental Engineering, Pusan National University) ;
  • Kwon, Soo-Youn (Department of Environmental Engineering, Pusan National University) ;
  • Choi, Won-Seok (Bioentech company, Ltd.) ;
  • Byun, Im-Gyu (Department of Environmental Engineering, Pusan National University) ;
  • Park, Tae-Joo (Department of Environmental Engineering, Pusan National University)
  • Received : 2007.10.12
  • Accepted : 2007.11.08
  • Published : 2007.11.30

Abstract

The upflow Biobead$^{(R)}$ process, one of biological aerated filters (BAF), which was used commercially, invented for removal of organic materials and nitrification. This process was modified to enhance the ability of denitrification through the induction of pre-anoxic tank. In this research, we investigated the effects of hydraulic retention time (HRT) and backwashing period in aerobic tank. The characteristics of nitrifying bacteria, which are composed of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), also investigated using fluorescence in situ hybridization (FISH). Even though the HRT was shortened, the efficiency of nitrification was not decreased when the organic loading rate and ammonium-nitrogen loading rate were $2.10kg/m^3/day$ and $0.25kg/m^3/day$, respectively. And then the distribution ratios of AOB and NOB showed the similar patterns. However, when the backwashing period was lengthened from 12 hours to 24 hours in aerobic 1 tank, the nitrification efficiency was decreased to 63.9% from 89.2%. The results of FISH explained that this decrease of nitrification efficiency was caused by the decrease of distribution ratio of AOB in aerobic 1 tank. The nitrification efficiencies of aerobic 1 and aerobic 2 tank were increased when the backwashing period was lengthened because of relative high distribution ratios of nitrifying bacteria.

Keywords

Acknowledgement

Supported by : 한국환경기술진흥원

References

  1. 강정구, 이충곤, 나용훈, 최의소, BAF를 이용한 영양소 제거, 한국수질보전학회 추계학술발표회 초록집, pp. 119-122 (1997)
  2. 건설신기술, 제289호, http://www.bioentech.com (accessed Jul. 2001.)
  3. 박정진, 정영록, 유재철, 허성호, 최원석, 변임규, 이태호, 박태주, Modified BAF 공정을 이용한 독립적인 무산소조에서 탈질미생물 군집의 특성, 대한환경공학회지, 28(7), pp. 752-756 (2006)
  4. 유재철, 박정진, 허성호, 김유진, 변임규, 이태호, 박태주, 호기성 생물막 반응기에서 Ammonia Oxidizing Bacteria에 대한 DO 농도의 영향, 대한환경공학회지, 29(1), pp. 106-112 (2007)
  5. 이창근, 김정숙, 강임석, 이병헌, 고정 생물막 공법에서 부착미생물의 역세에 관한 연구, 한국환경과학회지, 6(3), pp. 219-224 (1997)
  6. 이태근, 오중교, 이용택, 김동진, 이규현, 상향류식 Biological Aerated Filter의 제지폐수처리특성 및 최적 역세조건에 관한 연구, 대한환경공학회지, 18(12), pp. 1547-158 (1996)
  7. 환경부, 수질오염공정시험방법 (2004)
  8. Alfreider, A., Pernthhaler, J., Amman, R., Sattler, B., Glockner, F. O., Wille, A. and Psenner, R., Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization, Appl. Environ. Microbial., 62(6), pp. 2138-2144 (1996)
  9. APHA, Standard methods for the examination of water and wasetwater, American Public Health Association, Washington, D.C. (1995)
  10. Daims, H., Maixner, F., Lucker, S., Stoecker, K., Hace, K. and Wagner, M., Ecophysiology and niche differentiation of Nitrospira-like bacteria, the key nitrite oxidizers in wastewater treatment plants, Water Science and Technology, 54(1), pp. 21-27 (2006)
  11. Daims, H., Bruhl, A., Amann, R., Schleifer, K. and Wagner, M., The Domain-specific Probe EUB338 is Insufficient for the Detection of all Bacteria:Development and Evaluation of a more Comprehensive Probe Set, Systematic and Applied Microbiology, 22(3), pp. 434-444 (1999) https://doi.org/10.1016/S0723-2020(99)80053-8
  12. He, S. B., Xue, G. and Kong, H. N., The performance of BAF using natural zeolite as filter media under conditions of low temperature and ammonium shock load, J. of Hazardous Materials, 143, pp. 291-295 (2007) https://doi.org/10.1016/j.jhazmat.2006.09.024
  13. Hur, S. H., Park, J. J., Kim, Y. J., Yu, J. C., Byun, I. G., Lee, T. H. and Park, T. J., Fluorescence in situ hybridization and INT-dehydrogenase activity test to assess the effect of DO concentration in aerobic biofilm reactor, Korean J. of Chem. Eng., 24(1), pp. 93-98 (2007) https://doi.org/10.1007/s11814-007-5015-2
  14. Jang, Am., Characterization of nitrifying biofilm through the technique of microelectrodes and fluorescence in situ hybridization(FISH), Ph.D. thesis, Department of Environmental Science & Engineering, Kwangju Institute of Science & Technology (2002)
  15. Mobarry, B. K., Wagner, M., Urbain, V., Rittmann, B. E. and Stahl, D. A. Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria, Appl. Environ. Microbiol., 62(6), pp. 2156-2162 (1996)
  16. Sagberg, P, P., Dau thillie and Hamon, M., Bio film reactors; Acompact solution for the upgrading of wastewater treatment plant, Wat. Sci. Tech., 26(3-4), pp. 733-742 (1992)
  17. Tchobanoglous, G., Burton, F. and Stensel, H., Fundamentals of biological treatment, Wastewater Engineering, 4th Ed, Mc Graw Hill, Inc. New York (2003)
  18. Tschui, M., Tertiary nitrification in aerated piolt biofilters, Wat. Sci. Tech., 29(10-11), pp. 53-60 (1994)
  19. Wagner, M. and Loy, A., Bacterial community composition and function in sewage treatment system, Biotechnology, 13, pp. 218-227 (2002)
  20. Wagner, M., Rath, G. H., Flood, J. and Amann, R., In situ analysis of nitrifying bacteria in sewage treatment plants, Water Science and Technology, 34(1), pp. 237-244 (1996)