• Korean Journal of Plant Resources
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• v.14 no.2
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• pp.157-162
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• 2001

Korean native Orostachys species collected from 22 regions investigated morphological characters.O. japonicus species(No.3∼No.9) were similar leaf morphology and characterized by lanceolate leaves, cuspidate leaf apex.O. malacophyllus species(No.12∼No. 17) were morphologically characterized by obovate leaves and acute leaf apex. This species could be differentiate from other species by no thorn and leaf outlines formed by densely populated red dots.O. iwarenge species(No.18∼No.22) were diversified, such as obovate and elliptical leaves with acute, obtuse and round leaf apices. However, this species could be differentiate from other species bl'no thorn and grey powdery green colored leaves. Species collected from Maemuldo(No. 10) and Pocheon(No. 11) was assumed that these 2 species could be the new species which were not named classified.

• Journal of Animal Science and Technology
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• v.48 no.5
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• pp.637-650
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• 2006

Genomic DNA isolated from two crucian carp species obtained from Yesan (Carassius auratus) and Dangjin (Carassius cuvieri) in Korea were amplified at several times by polymerase chain reaction (PCR). The amplified products were separated by agarose gel electrophoresis (AGE) with oligonucleotides decamer primer and stained with ethidium bromide. The seven arbitrarily selected primers OPC-11, OPC-14, OPC-18, OPD- 02, OPD-11, OPD-15 and OPD-20 generated the shared loci by each species, the polymorphic and specific loci. The seven primers generated the total 458 loci that can be scored from the crucian carp obtained in C. auratus species. 358 fragments were generated from the species obtained in C. cuvieri species. The size of DNA fragments varies from 150 to 1,600bp. The complexity of the banding patterns varies dramatically between the primers and two locations. In this study, 458 loci were identified in the crucian carp species from Yesan and 358 in the crucian carp species from Dangjin: 84 polymorphic loci (18.3%) in the C. auratus species and 48 (13.4%) in the C. cuvieri species. 154 shared loci by each species, the average 22 per primer, were observed in the C. auratus species and 187 loci, the average 26.7 per primer, in the Dangjin species. Based on the average bandsharing (BS) values of all samples, the similarity matrix ranged from 0.434 to 0.868 in the C. auratus species and from 0.449 to 0.924 in the C. cuvieri species. The average BS value was 0.641±0.013 within the C. auratus species and 0.684±0.013 within the C. cuvieri species. The average BS value between two crucian carp species 0.484 ± 0.007, ranged from 0.307 to 0.682. The BS value between the individual No. 09 and No. 16 was 0.682, which was the highest between two crucian carp species. Compared separately, the BS value of individuals within the C. cuvieri species was higher than the C. auratus species. The dendrogram obtained by the seven primers, indicates three genetic clusters: cluster 1 (AURATUS No. 01, 02, 03, 04, 05, 06, 07, 08, 09, 10 and 11), cluster 2 (CUVIERI No. 12, 13, 14, 15, 16, 17, 18, 19, 20 and 21) and cluster 3 (CUVIERI no. 22). The shortest genetic distance displaying significant molecular difference was between the individual AURATUS No. 09 and AURATUS No. 08 from Yesan (genetic distance=0.064). The longest genetic distance displaying significant molecular differences was between the individual CUVIERI No. 17 and AURATUS No. 11 between two crucian carp species (0.477). RAPD-PCR analysis has revealed the significant genetic distance between two crucian carp species pairs.(Key words: Carassius auratus, Carassius cuvieri, Crucian Carp, DNA Polymorphism, Genetic Distance)

• Korean Journal of Medicinal Crop Science
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• v.21 no.3
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• pp.191-196
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• 2013

The effect on the antioxidant activity and Nitric Oxide activity production inhibitory activity of Taraxacum has not been known. Therefore, phenolics and flavonoid contents were investigated from the ethanol extracts of five different Taraxacum species. The results showed that, among the five Taraxacum, T. hallaisanensis contains the highest total phenolic and flavonoid contents. When the antioxidant activity was measured by DPPH, $ABTS^+$ and reducing power activity, the free radical scavenging activity of T. hallaisanensis was also the highest among five Taraxacum species. However, measurement by CCK-8 assay in Raw264.7 cells indicated that the extracts of Taraxacum species have no effect on cell viability. Moreover, we also investigated the effect of Taraxacum species on NO scavenging activity in lipopolysaccharide (LPS)-stimulated Raw264.7 cells. The results clearly showed that Taraxacum species inhibited NO production, and the inhibitory effect of T. hallaisanensis was the strongest. The above results suggested that Taraxacum species affected the antioxidant and NO scavenging activity, and among the five species, antioxidant and NO scavenging activity assay of T. hallaisanensis was significantly higher than those of other four Taraxacum species. Therefore, T. hallaisanensis could be used as a potential drug with anti-oxidant and anti-inflammatory effect.

• Horticultural Science & Technology
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• v.19 no.2
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• pp.159-162
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• 2001

The genetic relationships of Korean native Orostachys species collected from various regions were analysed using random amplified polymorphic DNA (RAPD) method. Eighteen Orostachys species analysed with UPGMA were clustered into three groups A, B, and C. However, four species were not clustered into any group. O. iwarenge species in group A (No. 18-No. 22) showed low similarity with 66.3-73.9% according to the cluster analysis. O. malacophyllus species in group B (No. 12-No. 17) showed low similarity with 66.7-83.7% according to the cluster analysis. The similarity coefficient value of O. japonicus (No. 3-No. 8) except Anmyeondo collected variety (No. 9) showed higher level with 84.2-92.3% than O. iwarenge or O. malacophyllus. Therefore, O. japonicus is thought to be genetically stable, and have less regional variation.

• Journal of Ecology and Environment
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• v.38 no.4
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• pp.451-459
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• 2015

We investigated the pattern of floral diversity including naturalized plant species and three ecological factors (area, elevation and distance from mainland) of plant species on 53 uninhabited islands in Gyungsangnam-do, southeastern South Korea. A total of 206 taxa in 67 families were observed, and the species of Compositae was most common. Thirteen taxa in eight families of the naturalized plants were observed on 33 islands. The numbers of total plant species, area and elevation were significantly correlated, but no relationship with distance from the mainland was observed. In addition, no relationship was found among the numbers of naturalized plants, area and elevation. However, the average rate of naturalization on islands with different elevations differed significantly, indicating the smallest proportion of naturalized plant species was on high islands. Multiple regression of total species richness identified elevation as a significant factor, while no significant variables were correlated with naturalized plant species. Nonmetric multidimensional scaling (NMS) ordination identified three major variables, distance from mainland, number of naturalized plant species and elevation. These findings indicate that the geography of islands such as area and elevation affected the species richness of plants on uninhabited islands, while human disturbance had a greater effect than geography on the species richness of naturalized plants on islands in southeastern South Korea.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.1
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• pp.101-110
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• 2009

The vascular plants in the Mt. Hoemun were listed 541 taxa composed of 113 families, 354 genera, 473 species, 1 subspecies, 64 varieties and 3 forms. Divided into 541 taxa; woody plants were 151 taxa (27.9%) and herbaceous plants were 390 taxa (72.1%). Based on the list of rare plants by the Korea Forest Service, 10 taxa were recorded in the studied areas; Lilium distichum (Preservation priority order: No. 159), Lilium callosum (No. 191), Tricyrtis dilatata (No. 97), Iris ensata var. sponianea (No. 197), Gastrodia elata (No.9), Aristolochia contorta (No. 151), Paeonia obovata (No. 30: Legal protection species), Prunus yedoensis (No. 110 : Planted species), Viola albida (No. 202), Schpolia japonica (No. 208). Based on the list of Korean endemic plants, 15 taxa were recorded; Cephalotaxus koreana, Lilium amabile, Salix purpurea var. japonica, Pseudostellaria multiflora, Aconitum pseudolaeve var. erectum, Filipendula glaberrima, Weigela subsessilis, etc. Based on the list of approved for delivering overseas of plants, 10 taxa were recorded; Carex okamotoi, Lilium distichum, Clematis trichotoma, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha; etc. Specific plant species by floral region were total 4S taxa (8.3% of all 541 taxa of vascular plants); Gastrodia elata, Paeonia obovata in class V, Carex arenicola, Corydalis grandicalyx in class IV, 5 taxa (Spiraea salicifolia, Schpolia japonica, Asperula lasiantha, etc.) in class III, 7 taxa (Lilium distichum, Iris ensata var. spontanea, Caryopteris incana, etc.) in class II and 28 taxa (Camptosorus sibiricus, Orixa japonica, Lonicera praeflorens, etc.) in class I. The naturalized plants in the surveyed sites were 13 families, 38 genera, 43 species, 2 varieties, 45 taxa (Phleum pratense, Medicago sativa, Sonchus asper, etc.) and naturalization rate was 8.3% of all 541 taxa of vascular plants. Ambrosia artemisiifolia var. elatior an ecosystem disturbing wild plants have been increasing. Therefore, the counter plants for continuous control and conservation are needed on the ecosystem of Mt. Hoemun.

• ALGAE
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• v.18 no.3
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• pp.207-215
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• 2003

Dynamics of phytoplankton communities were investigated at five stations in Youngsan River from December 1995 to November 1996. Phytoplankton were identified to the total of 466 taxa, which were composed of 136 genera, 438 species, 27 varieties and 1 forma. The standing crops ranged 1,163-50,765 cells ${\cdot}ml^{-1}$ during the investigation periods. The variation of classes was in order to Chrysophyceae - Bascillariophyceae - Chlorophyceae at St. 1 and was only Bacillariophyceae at the other stations. The dominant species were 12 taxa including Aulacoseira ambigua, A. garnulata, Cyclotella meneghiniana, C. pseudostelligera, Chlorella vulgaris, Dinobryon sertularia, Flagilaria elliptica, Monoraphidium contortum, Micractinium pusillum, Nitzschia palea, Scenedesmus grahneisii and Stephanodiscus hantzschii f. tenuis. The standing crops of dominant species ranged from 10.6% to 94.7%. The genus Dinobryon in Chrysophyceae was dominant species at St. 1 in December 1995, but not recorded in the other months. The dominant species were composed with the planktonic diatoms from winter to spring and were the tychoplanktonic and the benthic species at St. 2-5 from summer to fall. The relationships between total standing crops and water temperature, pH, $NH_4$, $NO_2$, $NO_3$ and $PO_4$ showed low positive or negative coefficients. Stephanodiscus hantzschii f. tenuis had low positive or negative coefficients with water temperature, $NH_4$, $NO_2$, $NO_3$ and $PO_4$. The fluctuations of standing crops in the Youngsan river phytoplankton community were not caused by a single factor but controlled by the complex factors with interaction between phytoplankton community and envirowmental factors.

• Development and Reproduction
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• v.18 no.4
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• pp.287-292
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• 2014

In this study, seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously calculated. Euclidean genetic distances within- and between-species were also calculated by complete linkage method with the sustenance of the hierarchical dendrogram program Systat version 13. The genomic DNA isolated from herring (Clupea pallasii), Korean anchovy (Coilia nasus) and large-eyed herring (Harengula zunashi), respectively, in the Yellow Sea, were amplified several times by PCR reaction. The hierarchical dendrogram shows three chief branches: cluster 1 (PALLASII 01, 02, 03, 04, 06 and 07), cluster 2 (NASUS 08, 09, 10, 11, 12, 13 and 14), and cluster 3 (ZUNASHI 15, 16, 17, 18, 19, 20, 21 and PALLASII 05). In three clupeid species, the shortest genetic distance displaying significant molecular difference was between individual PALLASII no. 03 and PALLASII no. 02 (0.018). Individual no. 06 of PALLASII was most distantly related to NASUS no. 11 (genetic distance = 0.318). Individuals from herring (C. pallasii) species (0.920) exhibited higher bandsharing values than did individuals from Korean anchovy (C. nasus) species (0.872) (P<0.05). As a result, this PCR analysis generated on the genetic data displayed that the herring (C. pallasii) species was widely separated from Korean anchovy (C. nasus) species. Reversely, individuals of Korean anchovy (C. nasus) species were a little closely related to those of large-eyed herring (H. zunashi) species.

• ALGAE
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• v.19 no.4
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• pp.329-337
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• 2004

Monthly variations of phytoplankton communities were conducted to the investigation at five stations in mid and lower parts of the Nakdong River from December 1995 to November 1996. The phytoplankton communities were identified a total 456taxa which were composed of 136 genera, 427 species, 27 varieties and 2 forma. The standing crops of phytoplankton communities ranged from 2.7 to 52.8 (${\times}$10³)cells·ml$^{-1}$ during the investigation periods. Bacillariophyceae was the dominant classes at all stations. The dominant species were 7taxa and the standing crops of these ranged from 18.3 to 95.1% of the standing crops of phytoplankton communities. The dominant species in the mid parts (st. 1, 2) of this river system were benthic species such as Navicula gregaria, N. viridula var. rostellata and Nitzschia palea, however it was planktonic species (Stephan discus hantzschii, Golenkinia radiata) and meroplanktonic species (Aulacoseira ambigua, A. granulata) in the lower parts (st. 3, 4, 5). The seasonal variations of the dominant species in the lower parts were appeared to the planktonic species (S. hantzschii) from winter to spring, and were the meroplanktonic species (A. ambigua and A. granulata) in from summer to fall. The lower parts of the Nakdong river were entropic states because the dominant species were composed A. ambigua, A. granulate and S. hantzschii which were indicators of entropic water quality. The interrelationships between total standing crops and environmental factors(water temperature, pH, NH$_4$, NO$_2$, NO$_3$ and PO$_4$) were low positive or negative coefficients. S. hantzschii had low positive or negative relations with water temperature, NH$_4$, NO$_2$, NO$_3$ and PO$_4$. As the result, the variations of standing crops of phytoplankton were not caused by a single factor but controlled by the complex factors.

• Journal of Korean Society of Forest Science
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• v.85 no.2
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• pp.271-279
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• 1996

This study was conducted to find out the appropriate rates of mixed-seeding of native woody and herb species. It was carried out in greenhouse from 1994 to 1995. The early days germination individuals were $60no./m^2$ in case of woody species for mixed seeding treatment and the treatments were fertilization, soil surface treatment, seeding amount. The investigation factors are the number of individuals of woody and herb species, biomass, height, number of tillers, etc. The mean individuals of Lespedeza bicolor and Amorpha frutzcosa were investigated $1.14no./m^2$, $0.496no./m^2$ in 1995, and Spiraea prunifolia var. simpliciflora and Alnus hirsuta were withering to death. In 1995, the Individuals of Oenothera odorata, Themeda triandra var. japonica, Cymbopogon tortilis var. goeringii, Miscanthus sinensis, Artemisia princeps var. orientalis, Lespedeza cuneata, and Arundinella hirta were investigated $5.06no./m^2$, $1,072no./m^2$, $0no./m^2$, $412.53no./m^2$, $88.6no./m^2$, $8.9no./m^2$, $57.46no./m^2$, respectively, in case of herb species. The height and biomass of Lespedeza bicolor and Amorpha fruticosa showed very fast growth, and those of Themeda triandra var. japonica, Miscanthus sinensis had a similar tendency. For the changes in woody species, according to the seeding amount, there was no significant relationship between species, but It was highly significant in 1995. In case of the herb species, the relationship between the number of individuals and seeding amount was significant for both 1994 and 1995. The most number of tiller was Miscanthus sinensis(21), and showed Themeda triandra var. japonica, Arundinella hirta in descending order. For the mixed seeding of wood and herb species, the number of individuals, height, biomass, tiller of herb species diminished as the seeding amount of herb species increased. It can be concluded that using Lespedeza bicolor and Amorpha fruticosa for woody species and like Arundinella hirta in herb species will be efficient for revegetation measures. It seems, therefore, that the plants of revegetation methods will be used to Lespedeza bicolor, Amorpha fruticosa and Arundinella hirta, and further study is needed on the seeding amount.