Browse > Article
http://dx.doi.org/10.7783/KJMCS.2013.21.3.191

Effect of Five Korean Native Taraxacum on Antioxidant Activity and Nitric Oxide Production Inhibitory Activity  

Choi, Kyeong Hee (Department of Crop Science & Biotechnology, Chonbuk National University)
Nam, Hyeon Hwa (Department of Crop Science & Biotechnology, Chonbuk National University)
Choo, Byung Kil (Department of Crop Science & Biotechnology, Chonbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.21, no.3, 2013 , pp. 191-196 More about this Journal
Abstract
The effect on the antioxidant activity and Nitric Oxide activity production inhibitory activity of Taraxacum has not been known. Therefore, phenolics and flavonoid contents were investigated from the ethanol extracts of five different Taraxacum species. The results showed that, among the five Taraxacum, T. hallaisanensis contains the highest total phenolic and flavonoid contents. When the antioxidant activity was measured by DPPH, $ABTS^+$ and reducing power activity, the free radical scavenging activity of T. hallaisanensis was also the highest among five Taraxacum species. However, measurement by CCK-8 assay in Raw264.7 cells indicated that the extracts of Taraxacum species have no effect on cell viability. Moreover, we also investigated the effect of Taraxacum species on NO scavenging activity in lipopolysaccharide (LPS)-stimulated Raw264.7 cells. The results clearly showed that Taraxacum species inhibited NO production, and the inhibitory effect of T. hallaisanensis was the strongest. The above results suggested that Taraxacum species affected the antioxidant and NO scavenging activity, and among the five species, antioxidant and NO scavenging activity assay of T. hallaisanensis was significantly higher than those of other four Taraxacum species. Therefore, T. hallaisanensis could be used as a potential drug with anti-oxidant and anti-inflammatory effect.
Keywords
Antioxidant; Nitric Oxide (NO); Korean Native Taraxacum;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Bak JP, Son JH, Kim YM, Jung JH, Leem KH, Lee EY and Kim EH. (2011). Suppression of the inflammatory response in macrophages by Paeoniae Radix Rubra. Korean Journal of Medicinal Crop Science. 19:373-379.   과학기술학회마을   DOI   ScienceOn
2 Bogdan C. (2001). Nitric oxide and the immune response. Nature Immunology. 2:907-916.   DOI   ScienceOn
3 Chae SK, Kang GS, Ma SJ, Bang KW, Oh MW and Oh SH. (2002). Standard food analysis. Paju, Korea. p.381-382.
4 Choi KH, Kim HK, Kim JH, Choi ES, Shin JA, Lee SO, Chintharlapalli S, Safe S, Abdelrahim M, Kong G, Choi HS, Jung JY, Cho HT, Cho NP and Cho SD. (2010). The p38 MAPK pathway is critical for 5,5'-dibromodiindolylmethaneinduced apoptosis to prevent oral squamous carcinoma cells. European Journal of Cancer Prevention. 19:153-159.   DOI   ScienceOn
5 Chon SU. (2012). Antioxidant activity and cytotoxicity of different Taraxacum species in Korea. Korean Journal of Crop Science. 57:51-59.   과학기술학회마을   DOI   ScienceOn
6 Dawson TM and Dawson VL. (1995). Nitric oxide: Actions and pathological roles. The Neuro Scientist. 1:7-18.
7 Dessy C and Feron O. (2004). Pathophysiological roles of nitiric oxide: In the heart and the coronary vasculature. Current Medicinal Chemistry-Anti-Inflammatory&Anti-Allergy Agents. 3:207-216.   DOI   ScienceOn
8 Gally JA, Montague PR, Reeke GN and Edelman GM. (1990). The no hypothesis: possible effects of a short-lived, rapidly diffusible signal in the development and function of the nervous system. Proceedings of the National Academy of Sciences. 87:3547-3551.   DOI   ScienceOn
9 Goodman DL, McDonnell JT, Nelson HS, Vaughan TR and Weber RW. (1990). Chronic urticaria exacerbated by the antioxidant food preservatives, butylated hydroxyanisole(BHA) and butylated hydroxytoluene(BHT). Journal of Allergy and Clinicla Immunology. 86:570-575.   DOI
10 Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS and Tannenbaum SR. (1982). Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Analytical Biochemistry. 126:131-138.   DOI   ScienceOn
11 Hertog MG, Hollman PC and Van de Putte B. (1993). Content of potentially anticarcinogenic flavonoids of tea infusions, wines and fruit juices. Journal of Agricultural and Food Chemistry. 41:1242-1246.   DOI   ScienceOn
12 Hertog MG, Hollman PC and Venema DP. (1992). Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits. Journal of Agricultural and Food Chemistry. 40:1591-1598.   DOI
13 Hierholzer C, Harbrecht B, Menezes JM, Kane J, MacMicking J, Nathan CF, Peitzman AB, Billiar TR and Tweardy DJ. (1998). Essential role of induced nitric oxide in the initiation of the inflammatory response after hemorrhagic shock. Journal of Experimental Medicine. 187:917-928.   DOI   ScienceOn
14 Huang D, Ou B and Prior RL. (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry. 53:1841-1856.   DOI   ScienceOn
15 Im DY, Kim SH and Hor JR. (2011). A comparative study on antioxidative of extracts from Taraxacum coreanum and Taraxacum officinale. Journal of The Korean Society of Cosmetology. 17:554-549.
16 Im DY and Lee KI. (2011). Nitric oxide production inhibitory and scavenging activity and tyrosinase inhibitory activity of extracts from Taraxacum officinale and Taraxacum coreanum. Korean Journal of Medicinal Crop Science. 19:362-367.   과학기술학회마을   DOI   ScienceOn
17 Lee HH and Lee SY. (2008). Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extract. Korean Journal of Medicinal Crop Science. 16:79-85.   과학기술학회마을
18 Kang MK, Shin SR and Kim KS. (2002). Antioxidantive and free radical scavenging activity of water extract from dandelion (Taraxacum officinale). Korean Journal of Food Preservation. 9:253-259.
19 Lee SH, Park HJ, Han GJ, Cho SM and Rhin SG. (2004a). A study of the nutritional composition of the dandelion by part(Taraxacum officinale). Korean Journal Community Living Science. 15:57-61.   과학기술학회마을
20 Lee SH, Park HJ, Kim YI and Rhin SG. (2004b). Comparison of the antioxidative and antimicrobial activity of different varieties and parts of dandelions. Korean Journal Community Living Science. 15:101-107.   과학기술학회마을
21 Leem DH, Choi KH, Han HS, Kim JH, Shin JA, Choi ES, Shim JH, Kong G, Min YK, Nam JS, Oh SH, Kim KA, Kwon KH, Cho NP and Cho SD. (2010). KO-202125, a sauristolactam derivate, induces apoptosis to prevent KB human oral squamous carcinoma cells through inhibition of cyclooxygenase-2 expression. European Journal of Cancer Prevention. 19:23-30.   DOI   ScienceOn
22 Lowenstein CJ, Dinerman JL and Snyder SH. (1994). Nitric oxide: A physiologic messenger. Annals of Internal Medicine. 120:227-237.   DOI   ScienceOn
23 Luss H, Nussler NC, Beger HG and Nussler AK. (1996). Expression and detection of inducible nitric oxide synthase in experimental models of inflammation. Methods. 10:51-60.   DOI   ScienceOn
24 Osanai T, Gujiwara N, Saitoh M, Sasaki S, Tomita H, Nakamura M, Osawa H, Yamabe H and Okumura K. (2002). Relationship between salt intake, nitric oxide and asymmetric dimethylarginine and its relevance to patients with end-stage renal disease. Blood Purification. 20:466-468.   DOI   ScienceOn
25 Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM and Pridham JB. (1995). The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research. 22:375-383.   DOI   ScienceOn
26 Osawa T. (1994). Novel natural antioxidants for utilization in food and biological systems. Post harvest biochemistry of plant foodmaterials in the tropics. Japan Scientific Societies Press. Tokyo, Japan. p.241-251.
27 Oyaizu M. (1986). Studies on products of browning reaction: Antioxidant activities of products of browning reaction prepared from glucosamine. Japanes Journal of Nutrition. 44:307-315.   DOI
28 Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237.   DOI   ScienceOn
29 Schutz K, Carle R and Schiebar A. (2006). Taraxacum-A review on its phytochemical and pharmacological profiles. Journal of Ethnopharmacology. 107:313-323.   DOI   ScienceOn
30 Velioglu Y, Mazza G, Gao L and Oomah B. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry. 46:4113-4117.   DOI   ScienceOn