• Title/Summary/Keyword: NO%24_2%24

Search Result 8,853, Processing Time 0.034 seconds

Effect of Ethanol Extracts from Defatted Perilla frutescens on LPS-induced Inflammation in Mouse BV2 Microglial Cells

  • Lee, Sung-Gyu;Kang, Hyun
    • Biomedical Science Letters
    • /
    • v.24 no.4
    • /
    • pp.398-404
    • /
    • 2018
  • To evaluate the antioxidant and anti-neuroinflammatory effects of defatted Perilla frutescens extract (DPE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Cell viabilities were estimated by MTT assay. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators such as nitric oxide (NO), inducible NO synthase (iNOS), Cyclooxygenase-2 (COX-2), and prostaglandin $E_2$ ($PGE_2$). Pretreatment with DPE prior to LPS treatment significantly inhibited excessive production of NO (10, 25, 50, 75, and $100{\mu}g/mL$) in a dose-dependent manner, and was associated with down regulation of expression of iNOS and COX-2. DPE also suppressed the LPS-induced increase in $PGE_2$ level (10, 25, 50, 75, and $100{\mu}g/mL$) in BV-2 cells. Therefore, DPE can be considered as a useful therapeutic and preventive approach for the treatment of several neurodegenerative diseases.

Geochemical Characteristics and Assesment of Nitrate Nitrogen in Groundwater in Yanggu-Gun, Gangwon-Do in Korea

  • Choi, Won Gyu
    • Journal of Soil and Groundwater Environment
    • /
    • v.24 no.6
    • /
    • pp.26-32
    • /
    • 2019
  • An analysis of groundwater quality is significant for monitoring and managing water contamination and groundwater system. For the purpose of those, the geochemical characteristics of groundwater were studied over the concern for water quality, water type and origin of nitrate nitrogen. Total colony counts were detected in 11 out of 20 samples, and the average value was 31.73 CFU/ml. Range and average of NO3-N concentrations were 0.9~24.0 mg/L and 8.3 mg/L. All groundwater types were found to be Ca2+-HCO3-. The range and average of NO3-N were 0.2~17.4 mg/L and 8.7 mg/L, and those of δ15N were 1.7~8.9‰, and 5.0‰. Careful consideration is required for evaluating the origin of nitrogen when NO3-N concentration is low. In general, noticeable difference between rockbed and alluvial water was not found. The ranges of nitrate origins by chemical fertilizer, livestock manure and domestic sewage, and natural soil were 29.6~76.4%, 14.2~58.9% and 2.6~7.0%, and the average values of those were 57.4%, 37.4%, and 5.3%, respectively. Origin of nitrate was affected by more chemical fertilizer than the other parameters. Rockbed water was more affected by chemical fertilizer than alluvial water.

NO2 gas sensor using an AlGaN/GaN Heterostructure FET with SnO2 catalyst deposited by ALD technique (원자막증착법(ALD) SnO2 촉매를 적용한 AlGaN/GaN 이종접합 트랜지스터 NO2 가스센서)

  • Yang, Suhyuk;Kim, Hyungtak
    • Journal of IKEEE
    • /
    • v.24 no.4
    • /
    • pp.1117-1121
    • /
    • 2020
  • In this work, it was confirmed that a SnO2 catalyst deposited by an atomic layer deposition(ALD) process can be employed in AlGaN/GaN heterostructure FET to detect NO2 gas. The fabricated HFET sensors on AlGaN/GaN-on-Si platform demonstrated that the devices with or without n-situ SiN have sensitivity of 5.5 % and 38 % at 200 ℃, respectively with response to 100 ppm-NO2.

Study on Inhibition of Microorganism Derived from Bovine Frozen Semen in In Vitro Culture of Bovine Follicular Oocytes (소 난포란의 체외배양에 있어서 소 동결정액유래 미생물 억제에 관한 연구)

  • 이성학;정구민;이종호;가학현;임경순
    • Korean Journal of Animal Reproduction
    • /
    • v.19 no.1
    • /
    • pp.1-7
    • /
    • 1995
  • This study was accomplished to illuminate factors of contamination of microbes in the culture medium and effect of antibiotics on prevention of contamination in the medium when bovine follicular oocyte was matured, fertilized and developed in vitro. 1. When washed or unwashed semen diluted with TCM 199 was incubated for 24∼72hr, contamination was come out. 2. When diluted semen with TCM 199 which has penicillin, streptomycin, gentamycin, kanamycin or nystatin was incubated for 24∼72hr, contamination was not come out only in kanamycin. 3. When imported semen which was diluted in TCM 199 with penicillin, streptomycin, gentamycin, kanamycin or nystatin was incubated for 24∼72hr, contamination was not come out in all treatments. 4. When semen which was diluted in BO, CZB, Ham's F10 or TCM 199 was incubated for 24∼72hr, kanamycin showed no contamination in all treatments, but gentamycin showed contamination in CZB, Ham's F10 and TCM 199. 5. When the semen diluted in BO was moved at 24hr after incubation into BO and incubated for 72hr, contamination was not come out, but when it was moved into the TCM 199 and incubated for 72hr, contamination was come out at 48 to 72hr of incubation. 6. When the semen diluted in BO, BO+BSA or BO+FBS containing gentamycin, kanamycin or nystatin was incubated for 24∼72hr, the diluted semen in BO or BO+BSA showed no contamination in all antibiotics but the diluted semen in BO+FBS showed no contamination only in kanamycin. 7.The Pseudomonas cepacia, Serratia liquefaciens, Klebsiella pneumaniae was respectively isolated in the semen of A, B, and C bull and the microbes are highly affected by amikacin, tobramycin and kanamycin. 8. When bovine folicular oocyte was in vitro matured, fertilized and developed in the simple medium with kanamycin, 26.6% was developed to over 32cell stage embryo.

  • PDF

Changes In Concentrations of Urea-N, NH4-N and NO3-N in Percolating Water During Rice Growing Season (수도재배포장에서 침투수의 Urea-N, NH4-N 및 NO3-N의 농도변화)

  • Lee, Sang-Mo;Yoo, Sun-Ho;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.28 no.2
    • /
    • pp.160-164
    • /
    • 1995
  • This study was conducted to obtain quantitative data on the behavior of surface-applied urea to a paddy field which would help to protect against environmental pollution as well as to increase the efficiency of nitrogen fertilizer. The percolating water samples were collected with porous ceramic cups installed at 25, 50 and 75cm depths in a paddy field during the rice growing season(June 1992-September 1992) and analyzed for urea-N. NHAN and $NO_3-N$. In the paddy field to which urea fertilizer was applied at the rates of 12 and 24kg N/10a, the surface-applied urea was detected even at 75cm depth as the form of urea-N upto 12days after application. The maximum concentrations of urea-N in the percolating water sampled at 25, 50 and 75cm depths were the same irrespective of soil depth and the values were 0.06 and $0.12{\mu}g/m{\ell}$ for the application rates of 12 and 24kg N/10a respectively. The concentrations of $NH_4-N$ gradually decreased with time during the vegetative growth period : thereafter. the concentrations remained nearly constant. The maximum concentrations of $NH_4-N$ at 25cm depth were 1.2 and $5.6{\mu}g/m{\ell}$ for 12 and 24kg N/10a rate respectively. The $NO_3-N$ concentrations of percolating water ranged 0.1~0.5 and $0.2{\sim}0.5{\mu}g/m{\ell}$ for urea application rates of 12 and 24kg N/10a respectively. The nitrate concentration data suggest that nitrification process occurred continuously in paddy field during the rice growing season.

  • PDF

Comparison between 24 hr pH Monitoring and Esophageal Scintigrapy in Children Presenting with Gastroesophageal Reflux Symptoms (식도 동위 원소 검사와 24시간 식도 pH 검사와의 비교 연구)

  • Wi, Ho Sung;Kim, Yong Joo;Seol, In Joon;Choi, Yun Young
    • Pediatric Gastroenterology, Hepatology & Nutrition
    • /
    • v.7 no.2
    • /
    • pp.163-169
    • /
    • 2004
  • Purpose: Many diagnostic modalities have been used for diagnosis of gastroesophageal reflux disease (GERD). Feeding materials during esophageal pH monitoring may interfere the result of examination and esophageal pH monitoring can not diagnose GER in case of alkaline reflux. The purpose of our study is to evaluate whether scintigraphy can substitiute 24 hr pH monitoring in children with GERD. Methods: From March 2002 to June 2003, 23 patients (12 boys and 11 girls, mean age 27 months) who have been admitted to Hanyang University Hospital presented with GER symptoms (recurrent vomiting, cough, chest pain, irritability) were included in the study. Scintigraphy and 24 hr pH monitoring were performed in all patients. Results: Six out of the 23 patients (26.1%) had evidence of GER on 24 hr pH monitoring, whereas nine of 23 patients (39.2%) exhibited GER by scintigraphy. Two out of the 23 patients could not be tested because of irritability. Three (14.3%) patients had evidence of GER on both 24 hr pH monitoring and scintigraphy, three (14.3%) patients on only 24 hr pH monitoring, six (28.6%) patients on only scintigraphy, and nine (42.9%) patients had no evidence of GER on both methods. No correlation was observed between 24 hr pH monitoring and scintigraphic results by Fisher's exact test (p=0.523) or Kendal's tau (t=0.678). Conclusion: The results of this study demonstrated that there was no correlation between 24 hr pH monitoring and scintigraphy. Therefore these modalities could be used as complementary tests to diagnose GERD.

  • PDF

AN EXPERIMENTAL STUDY OF BOND STRENGTH OF GLASS IONOMER LUTING CEMENT UNDER THE FUNCTIONAL LOADS ON THE CROWN (기능 하중 하에서 접착용 Glass Ionomer 시멘트의 결합강도에 대한 비교연구)

  • Jun Joong-Hyun;Kang Woo-Jin;Lee Keun-Woo
    • The Journal of Korean Academy of Prosthodontics
    • /
    • v.30 no.2
    • /
    • pp.225-232
    • /
    • 1992
  • The purpose of this study was to compare the bond strength of the glass ionomer luting cements after the functional loads were applied to the crowns respectively 10 min, 30 min, 1 hr, 24 hrs later after mixing of cements. The conventional (GC Fuji I; GC Int. Co., Japan), water hardening type (Ketac Cem; ESPE, W. Germany) glass ionomer luting cement and C-dent ZPC (Confi-Dental Products Co., U.S.A.) as a control were used. The results of this study were obtained as follows: 1. In the bond strength of GC Fuji I, 24 hr-group was highest and there were no statistically significant differences among 10 min-, 30 min- and 1 hr- groups. 2. l3 the bond strength of Ketac Cem and C-dent ZPC, there was no statistically significant differences among 10 min-, 30 min-, 1 hr- and 24 hr-groups respectively. 3. Comparing the bond strength among cements in 10 min-, 30 min-groups respectively, GC Fuji I was lowest and there was no statistically significant difference between Ketac Cem and C-dent ZPC. 4. The bond strengths between GC Fuji I and Ketac Cem were not significantly different in 1 hr-, 24 hr-group. 5. The bond strengths among 3 types of cements were not significantly different in 24 hr-groups.

  • PDF

Nonlinear analysis of cardiotonic effect of acupuncture treatment on heart rate variability assessed by 24-hour Holter monitoring (침처치의 24시간 심박변이도 영향에 대한 비선형 분석)

  • Oh, Dal-Seok;Lee, Jeon;Kim, Jong-Yeol;Choi, Sun-Mi
    • Korean Journal of Oriental Medicine
    • /
    • v.14 no.1
    • /
    • pp.85-89
    • /
    • 2008
  • This study is to investigate cardiotonic effect of acupuncture on heart rate variability(HRV) analyzed by a nonlinear way(DFA, Detrended Fluctuation Analysis). It was designed as a randomized, single-blind, waiting list-controlled, cross-over study. We assessed heart rate and R-R intervals in Circadian electrocardiography with a Holter monitoring device for twelve hospitalized participants. The compatible analytical program, Zymed, was used for generating the signals of R-R intervals from 24 hour-ECG. In DFA analysis, we produced DFA alpha 1, alpha 2 parameters according to the process of Cygwin module of Linux server. We tested if there was any difference between HRV parameters using SPSS, a statistical package. There was no difference between acupuncture and no treatment group in DFA alpha 2 parameter {95% Confidence Interval (-)0.058 - 0.037, P = .565}. Two group all showed large intra-individual variations. Consequently, acupuncture treatment did not modulate the complexity of HRV in a DFA analysis. This study can be a rationale for acupuncture's properties on cardiovascular and autonomic systems.

  • PDF